scholarly journals Vaginal health in women and the possibility of predicting preterm birth through microbiome analysis

2021 ◽  
Vol 64 (12) ◽  
pp. 833-840
Author(s):  
Young Min Hur ◽  
Mi Na Kang ◽  
Young Ju Kim
Keyword(s):  
Preterm Birth ◽  
Lactic Acid Production ◽  
Premenopausal Women ◽  
The Body ◽  
Lactobacillus Species ◽  
Next Generation Sequencing Technology ◽  
Microbiome Composition ◽  
Microbiome Analysis ◽  
Prediction And Prevention ◽  
Vaginal Health

Background: With the recent development of next-generation sequencing technology, the microbiome in the body is being revealed in detail. It is also possible to describe the normal vaginal microenvironment and, more specifically, any changes in pregnancy. Moreover, we present the hypothesis that the microbiome is a contributing factor to preterm birth (PTB).Current Concepts: High estrogen status stimulates the maturation and proliferation of vaginal epithelial cells and the accumulation of glycogen, which promotes lactic acid production and maintains the vaginal environment at an acidic pH. The vaginas of most premenopausal women are predominantly colonized by Lactobacillus which plays an important role in local defense. Recently, it has also been reported that there are several specific types of Lactobacillus species, while other anaerobes, including Gardnerella and Atopobium also coexist in the vagina. Vaginal dysbiosis is defined as various expressions of microorganisms, secretion of specific metabolites, and changes in pH. During pregnancy, a multitude of microbiome changes occur in the oral cavity, gut, vagina, and placenta. The risk of PTB increases if the microbiome changes to one of dysbiosis. It is possible to analyze the characteristic microbiome composition related to PTB and to develop biomarkers predicting PTB. It is necessary to educate patients based on these findings.Discussion and Conclusion: Microbiome analysis has contributed significantly to understanding the association between women’s vaginal health and PTB. Continued research will also contribute to public health by assisting in the prediction and prevention of PTB.

scholarly journals The role of lactic acid production by probiotic Lactobacillus species in vaginal health

2017 ◽  
Vol 168 (9-10) ◽  
pp. 782-792 ◽  
Author(s):  
Gilda Tachedjian ◽  
Muriel Aldunate ◽  
Catronia S. Bradshaw ◽  
Richard A. Cone
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactobacillus Species ◽  
Probiotic Lactobacillus ◽  
Vaginal Health

scholarly journals Microbiota of the Tongue and Systemic Connections: The Examination of the Tongue as an Integrated Approach in Oral Medicine

Hygiene ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 56-68
Author(s):  
Cinzia Casu ◽  
Giovanna Mosaico ◽  
Valentino Natoli ◽  
Antonio Scarano ◽  
Felice Lorusso ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Survival Rates ◽  
Integrated Approach ◽  
Potential Method ◽  
The Body ◽  
Oral Medicine ◽  
Next Generation Sequencing Technology ◽  
Advanced Phase ◽  
Holistic Concept ◽  
Unique Method

The tongue is able to quickly reflect the state of health or disease of the human body. Tongue inspection is an important diagnostic approach. It is a unique method that allows to explore the pathogenesis of diseases based on the guiding principles of the holistic concept that involves the observation of changes in the lining of the tongue in order to understand the physiological functions and pathological changes of the body. It is a potential method of screening and early detection of cancer. However, the subjective inspection of the tongue has a low reliability index, and therefore computerized systems of acquisition of diagnostic bioinformation have been developed to analyze the lining of the tongue. Next-generation sequencing technology is used to determine the V2–V4 hypervariable regions of 16S rRNA to study the microbiota. A lot of neoplasms are identified only at an advanced phase, while in the early stages, many subjects remain in an asymptomatic form. On the contrary, the early diagnosis is able to increase the prognosis of cancer and improve the survival rates of subjects. Evidently, it is necessary to develop new strategies in oral medicine for the early diagnosis of diseases, and the diagnosis of the tongue as a minimally invasive method is certainly one of them.

scholarly journals Seasonal migrations, body temperature fluctuations, and infection dynamics in adult amphibians

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4698 ◽  
Author(s):  
David R. Daversa ◽  
Camino Monsalve-Carcaño ◽  
Luis M. Carrascal ◽  
Jaime Bosch
Keyword(s):  
Seasonal Variation ◽  
Body Temperature ◽  
Annual Cycle ◽  
Batrachochytrium Dendrobatidis ◽  
Effective Means ◽  
The Body ◽  
Infection Prevalence ◽  
Body Temperatures ◽  
Annual Cycles ◽  
Prediction And Prevention

Risks of parasitism vary over time, with infection prevalence often fluctuating with seasonal changes in the annual cycle. Identifying the biological mechanisms underlying seasonality in infection can enable better prediction and prevention of future infection peaks. Obtaining longitudinal data on individual infections and traits across seasons throughout the annual cycle is perhaps the most effective means of achieving this aim, yet few studies have obtained such information for wildlife. Here, we tracked spiny common toads (Bufo spinosus) within and across annual cycles to assess seasonal variation in movement, body temperatures and infection from the fungal parasite, Batrachochytrium dendrobatidis (Bd). Across annual cycles, toads did not consistently sustain infections but instead gained and lost infections from year to year. Radio-tracking showed that infected toads lose infections during post-breeding migrations, and no toads contracted infection following migration, which may be one explanation for the inter-annual variability in Bd infections. We also found pronounced seasonal variation in toad body temperatures. Body temperatures approached 0 °C during winter hibernation but remained largely within the thermal tolerance range of Bd. These findings provide direct documentation of migratory recovery (i.e., loss of infection during migration) and escape in a wild population. The body temperature reductions that we observed during hibernation warrant further consideration into the role that this period plays in seasonal Bd dynamics.

scholarly journals Identification and Sensitivity of Vaginal and Probiotic Lactobacillus species to Urinary Antibiotics

2020 ◽  
Vol 12 (02) ◽  
pp. 111-114
Author(s):  
Lipika Singhal ◽  
Varsha Gupta ◽  
Menal Gupta ◽  
Poonam Goel ◽  
Jagdish Chander
Keyword(s):  
Vaginal Microbiota ◽  
Lactobacillus Species ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Urogenital Infections ◽  
Probiotic Lactobacillus ◽  
Probiotic Strains ◽  
Vaginal Health ◽  
Uropathogenic Bacteria ◽  
Lactobacillus Spp

Abstract Objectives Healthy vaginal microbiota is mainly dominated by Lactobacillus species namely L. crispatus, L. gasseri, L. jensenii, and L. iners. Lactobacilli are thought to play an important role in the prevention of urogenital infections, and Lactobacillus probiotics to restore and/or maintain vaginal health has been advocated. These can interfere with the adherence, growth, and colonization by uropathogenic bacteria, thus reducing the risk of urinary tract infection (UTI). This study aims to isolate and evaluate the susceptibility of healthy vaginal and probiotic Lactobacillus spp. to urinary antibiotics. Materials and methods A total of 50 premenopausal, nonmenstruating women with no symptoms of vaginal infection or UTI or antimicrobial use in the past 2 weeks were enrolled. Two high vaginal swabs were collected for Nugent’s scoring and anaerobic culture. Colonies yielding gram-positive rods were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Lactobacillus strains in a vaginal probiotic tablet were also isolated, and the sensitivity of both sets to urinary antibiotics was determined. Results A total of 29 Lactobacillus spp. were isolated, including L. crispatus (15), L. gasseri (5), L. vaginalis (4), L. paracasei (2), L. curvatus (1), L. fermentum (1), and L. paraplantarum (1). All strains were susceptible to nitrofurantoin and resistant to norfloxacin, gentamicin, and cotrimoxazole (except L. paracasei). The probiotic strains were pan-sensitive. Conclusions Prophylactic antibiotics are capable of eliminating the normal vaginal inhabitants, which may increase the probability of UTI. The administration of vaginal probiotics as an alternate or multidrug therapy can restore vaginal microbiota and help prevent recurrent UTI.

scholarly journals Menopause Delays the Typical Recovery of Pre-Exercise Hepcidin Levels after High-Intensity Interval Running Exercise in Endurance-Trained Women

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3866
Author(s):  
Víctor Alfaro-Magallanes ◽  
Pedro Benito ◽  
Beatriz Rael ◽  
Laura Barba-Moreno ◽  
Nuria Romero-Parra ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
High Intensity ◽  
Iron Homeostasis ◽  
Premenopausal Women ◽  
The Body ◽  
Peak Oxygen Consumption ◽  
Post Exercise ◽  
Running Exercise ◽  
Maximal Aerobic Speed ◽  
High Intensity Interval

Menopause commonly presents the gradual accumulation of iron in the body over the years, which is a risk factor for diseases such as cancer, osteoporosis, or cardiovascular diseases. Running exercise is known to acutely increase hepcidin levels, which reduces iron absorption and recycling. As this fact has not been studied in postmenopausal women, this study investigated the hepcidin response to running exercise in this population. Thirteen endurance-trained postmenopausal women (age: 51.5 ± 3.89 years; height: 161.8 ± 4.9 cm; body mass: 55.9 ± 3.6 kg; body fat: 24.7 ± 4.2%; peak oxygen consumption: 42.4 ± 4.0 mL·min−1·kg−1) performed a high-intensity interval running protocol, which consisted of 8 × 3 min bouts at 85% of the maximal aerobic speed with 90-second recovery. Blood samples were collected pre-exercise, 0, 3, and 24 hours post-exercise. As expected, hepcidin exhibited higher values at 3 hours post-exercise (3.69 ± 3.38 nmol/L), but also at 24 hours post-exercise (3.25 ± 3.61 nmol/L), in comparison with pre-exercise (1.77 ± 1.74 nmol/L; p = 0.023 and p = 0.020, respectively) and 0 hour post-exercise (2.05 ± 2.00 nmol/L; p = 0.021 and p = 0.032, respectively) concentrations. These differences were preceded by a significant increment of interleukin-6 at 0 hour post-exercise (3.41 ± 1.60 pg/mL) compared to pre-exercise (1.65 ± 0.48 pg/m, p = 0.003), 3 hours (1.50 ± 0.00 pg/mL, p = 0.002) and 24 hours post-exercise (1.52 ± 0.07 pg/mL, p = 0.001). Hepcidin peaked at 3 hours post-exercise as the literature described for premenopausal women but does not seem to be fully recovered to pre-exercise levels within 24 hours post-exercise, as it would be expected. This suggests a slower recovery of basal hepcidin levels in postmenopausal women, suggesting interesting applications in order to modify iron homeostasis as appropriate, such as the prevention of iron accumulation or proper timing of iron supplementation.

scholarly journals A novel sequencing-based vaginal health assay combining self-sampling, HPV detection and genotyping, STI detection, and vaginal microbiome analysis

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0215945 ◽  
Author(s):  
Elisabeth M. Bik ◽  
Sara W. Bird ◽  
Juan P. Bustamante ◽  
Luis E. Leon ◽  
Pamela A. Nieto ◽  
...  
Keyword(s):  
Vaginal Microbiome ◽  
Microbiome Analysis ◽  
Vaginal Health

scholarly journals Lactic acid production from cane molasses

2017 ◽  
Vol 2 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Aladár Vidra ◽  
András József Tóth ◽  
Áron Németh
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Acid Production ◽  
Animal Feed ◽  
Sugar Content ◽  
Lactic Acid Production ◽  
Raw Material ◽  
Lactobacillus Species ◽  
Alcohol Production ◽  
Study Results

Abstract Molasses, a by-product of the sugar manufacturing process, generally comprises approximately 50% (w/w) of total sugars, but it is currently used primarily [1] as an animal feed and as a raw material in alcohol production. Currently, the sugar production is more than 160 million tones worldwide. Its byproduct molasses contain heavy metals which have growthinhibitory effect. The main sugar content in molasses is sucrose which often need to be hydrolyzed to glucose and fructose especially for utilization by Lactobacillus species. Lactobacillus species can convert sugar content to lactic acid with great efficiency, which is a valuable chemical. Lactic acid production from sugar molasses using batch fermentations with Lactobacillus casei and Lactobacillus sp. MKT878 were investigated in this study. Results showed, that both examined Lactobacillus species could grow on molasses despite the heavy metals inhibitory effects. The conversion of sugar content to lactic acid was successful with yield between 55-80 g/g.

Sign in / Sign up

Export Citation Format

Share Document