Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

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In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽

10.51248/.v39i2.191 ◽

2020 ◽

Vol 39 (2) ◽

pp. 258-262

Author(s):

M. Narmatha ◽

S. Maneemegalai

Keyword(s):

Antioxidant Activity ◽

Ethanol Extract ◽

Total Phenol Content ◽

Scavenging Activity ◽

Syzygium Cumini ◽

Total Alkaloids ◽

Dpph Scavenging Activity ◽

Inhibitory Activities ◽

Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.

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Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i830406 ◽

2021 ◽

pp. 1-9

Author(s):

Gerard Bessan Dossou-Agoin ◽

Habib Ganfon ◽

Fidèle Assogba ◽

Adam Gbankoto ◽

Joachim Gbenou ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Aqueous Extracts ◽

Fruit Extract ◽

Stem Extract ◽

Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽

10.35799/pha.9.2020.29268 ◽

2020 ◽

Vol 9 (2) ◽

pp. 178

Author(s):

Julia Elsa Lakoro ◽

Max R. J. Runtuwene ◽

Paulina V. Y. Yamlean

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Compounds ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of 53,34 mg / L. Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

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The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

Jurnal Teknologi ◽

10.11113/jt.v68.3030 ◽

2014 ◽

Vol 68 (5) ◽

Cited By ~ 1

Author(s):

Hafizah Mohd Hadzri ◽

Mohd Azizi Che Yunus ◽

Salman Zhari ◽

Fahim Rithwan

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Soxhlet Extraction ◽

Solvent Polarity ◽

Extraction Methods ◽

The Other ◽

Natural Antioxidant ◽

Total Phenolic

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.

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Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity

Antioxidants ◽

10.3390/antiox8100425 ◽

2019 ◽

Vol 8 (10) ◽

pp. 425 ◽

Cited By ~ 4

Author(s):

Mengyang Hou ◽

Wenzhong Hu ◽

Aosheng Wang ◽

Zhilong Xiu ◽

Yusheng Shi ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Pteris Cretica ◽

Nitric Oxide Radical ◽

Ultrasonic Assisted ◽

Ic50 Values

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from Pteris cretica L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50 values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from P. cretica.

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Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽

10.3390/antiox9060535 ◽

2020 ◽

Vol 9 (6) ◽

pp. 535 ◽

Cited By ~ 1

Author(s):

Francesco Gai ◽

Magdalena Karamać ◽

Michał A. Janiak ◽

Ryszard Amarowicz ◽

Pier Giorgio Peiretti

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Total Phenolic ◽

Growth Stages ◽

Scavenging Activity ◽

Flowering Stage ◽

Phenolic Profile ◽

Aerial Parts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

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Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽

10.3390/antiox8070200 ◽

2019 ◽

Vol 8 (7) ◽

pp. 200 ◽

Cited By ~ 5

Author(s):

Yue Zhou ◽

Xiao-Yu Xu ◽

Ren-You Gan ◽

Jie Zheng ◽

Ya Li ◽

...

Keyword(s):

Extraction Method ◽

Natural Antioxidants ◽

Extraction Time ◽

Antioxidant Compounds ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

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PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i6.31505 ◽

2020 ◽

pp. 55-58 ◽

Cited By ~ 1

Author(s):

YOHANES TANDORO ◽

PAINI SRI WIDYAWATI ◽

TARSISIUS DWI WIBAWA BUDIANTA ◽

GRACE SUMARGO

Keyword(s):

Antioxidant Activity ◽

Cardiac Glycosides ◽

Reducing Power ◽

Total Phenol ◽

Total Flavonoids ◽

Scavenging Activity ◽

Phytochemical Compounds ◽

Dpph Scavenging Activity ◽

Passiflora Foetida ◽

Dpph Scavenging

Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract. Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm. Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base. Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.

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Supercritical Fluid Extraction of Polyphenols from Vietnamese Callisia fragrans Leaves and Antioxidant Activity of the Extract

Journal of Chemistry ◽

10.1155/2020/9548401 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Le Thao My Phan ◽

Kim Thien Phuc Nguyen ◽

Hoai Thanh Vuong ◽

Do Dat Tran ◽

Tran Xuan Phuong Nguyen ◽

...

Keyword(s):

Antioxidant Activity ◽

Flow Rate ◽

Ethanol Concentration ◽

Soxhlet Extraction ◽

Extraction Temperature ◽

Total Polyphenol ◽

High Antioxidant Activity ◽

Medicinal Properties ◽

Temperature And Pressure ◽

Single Factor Experiment

Vietnamese Callisia fragrans (C. fragrans) has been considered as a valuable traditional plant with various medicinal properties. In this study, polyphenols were extracted from Vietnamese C. fragrans leaves by supercritical carbon dioxide (SC-CO2) extraction method using ethanol as a cosolvent. The investigation of four factors influencing the total polyphenol content (TPC) and antioxidant activity of the extracts obtained from each single-factor experiment was conducted including ethanol concentration, CO2 flow rate, extraction temperature, and pressure. Besides, the extraction efficiency of the SC-CO2 method under the best extraction conditions, namely ethanol concentration of 14%, CO2 flow rate of 20 g/min, extraction temperature of 45°C, and pressure of 200 bar was compared to that of the Soxhlet extraction (SE) method in terms of the TPC and antioxidant activity of the extracts. The results showed that using SC-CO2 method, the TPC and the half-maximal inhibitory concentration value obtained were of 87.42 ± 1.33 mg/g and 243.83 ± 5.30 μM TE/g, respectively, with much less time and solvent amount required while that obtained using SE method were of 85.34 ± 4.27 mg/g and 236.33 ± 7.66 μM TE/g, respectively. This indicated that SC-CO2 would be suitable for the industrial production of polyphenols with high antioxidant activity of the extracts obtained due to the restrictions of using the SE method and advantages of applying SC-CO2 method. Therefore, SC-CO2 method could be regarded as a potentially upcoming extraction technique which might be employed to replace the conventional SE method.

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Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein

BioMed Research International ◽

10.1155/2017/6837285 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 6

Author(s):

Xueqin Wang ◽

Huahua Yu ◽

Ronge Xing ◽

Xiaolin Chen ◽

Song Liu ◽

...

Keyword(s):

Antioxidant Activity ◽

Protein Hydrolysate ◽

Scientific Basis ◽

Enzyme Concentration ◽

Scavenging Activity ◽

Preparation Conditions ◽

Low Concentrations ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

The Stability

This study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85 U/g, pH of 7.00, temperature of 39.55°C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+and Fe3+were 5 mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+at a 5 mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+and Mg2+had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs.

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