scholarly journals Analysis and selection of an effective method for growing meristemic potato plants (SolanumTuberosum L.)

2021 ◽  
Vol 7 (Special) ◽  
pp. 14-14
Author(s):  
Boris Zherebtsov ◽  
◽  
Alexander Kozlov ◽  
Sergey Netesov
Keyword(s):  
Nutrient Medium ◽  
Traditional Methods ◽  
Potato Plants ◽  
Cultivation Methods ◽  
Selection Of

In this article, methods of growing meristemic plants are considered and the basics of hydroponics techniques are studied. Studies of the main characteristics of growing potato plants in a nutrient medium on the basis of a designed automated hydroponic installation are presented and the indicators are compared with traditional methods. Keywords: HYDROPONICS, GROUNDLESS CULTIVATION METHODS, HYDROPONIC AGRICULTURE, HYDROPONIC TECHNOLOGIES

scholarly journals Development of polyvalent toxoid Clostridium perfringens against anaerobic enterotoxaemia in young cattle and small ruminants

2021 ◽  
Vol 282 ◽  
pp. 04009
Author(s):  
F.Kh. Pulotov ◽  
O.D. Nazarova ◽  
N.A. Akhmadov ◽  
A.I. Karimzoda
Keyword(s):  
Nutrient Medium ◽  
Small Ruminants ◽  
Farm Animals ◽  
Drug Preparation ◽  
Type D ◽  
Industrial Strains ◽  
Type C ◽  
Young Cattle ◽  
C 30 ◽  
Selection Of

The authors of the article presents the results of the development of a polyvalent toxoid from C.perfringens strains against anaerobic enterotoxaemia of young cattle and small ruminants and an assessment of its effectiveness. It is presented the data on the selection of the recipe composition of the nutrient medium, the cultivation of industrial strains, the process of drug preparation, the selection of the optimal ratio of components and the method of controlling the effectiveness. Sequential cultivation of industrial strains of C.perfringens was carried out by cultivating them in flasks, bottles and bioreactors in the nutrient medium developed by the authors, consisting of: 12.0 g/l - liver extract; 25.0 g/l - casein-peptone; 3.2g/l –K2HPO4; 1.8 g/l – KH2PO4; 0.5 g/l – MgSO4. The development of the drug included the selection of optimal and balanced ratios of C. perfringens type A toxoids - 15 IU/ml, C.perfringens type B - 20 IU/ml, C.perfringens type C - 30 IU/ml, C. perfringens type D - 30 IU/ml, which ensured 100% protection of the immunized animals. Studies on laboratory and farm animals revealed the harmlessness, areactogenicity of the drug, which stimulated the development of immunity and resistance to the toxic effects of all strains of C.perfringens.

scholarly journals Analysis of Yield of Eleutherosides B and E inAcanthopanax divaricatusandA. koreanumGrown with Varying Cultivation Methods

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Jeong Min Lee ◽  
Myoung-Hee Lee ◽  
Suk-Bok Pae ◽  
Ki-Won Oh ◽  
Chan-Sik Jung ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Total Content ◽  
Planting Time ◽  
Material Development ◽  
Plant Section ◽  
Cultivation Methods ◽  
Selection Of

An analysis of the yield of eleutherosides B and E inAcanthopanax divaricatusandA. koreanumwas performed using high performance liquid chromatography to evaluate production by different cultivation methods. InA. divaricatusandA. koreanum, the total content of eleutherosides B and E was 2.466–7.360 mg/g varying by plant section, 3.886–11.506 mg/g by pinching site, 3.655–10.083 mg/g by planting time, and 3.652–10.108 mg/g by fertilizer ratio. Thus the total content of eleutherosides B and E inA. divaricatusandA. koreanumdiffered depending on cultivation methods. These results present useful information for high eleutheroside content applications inA. divaricatusandA. koreanum. This information can affect selection of plant section and cultivation methods for nutraceutical, pharmaceutical, and cosmeceutical material development.

Selection of a sterilization regime for the nutrient medium in lysine biosynthesis

1985 ◽  
Vol 19 (1) ◽  
pp. 71-74
Author(s):  
V. E. Matveev ◽  
V. E. Aksenovskaya ◽  
L. E. Petrosyan ◽  
A. �. Bulutyan ◽  
F. E. Nazaryan ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Lysine Biosynthesis ◽  
Selection Of ◽  
Sterilization Regime

scholarly journals Development of a probiotic for animals and aquaculture based on Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains

2021 ◽  
Vol 11 (3) ◽  
pp. 393-402
Author(s):  
A. V. Malkova ◽  
I. Yu. Evdokimov ◽  
M. V. Shirmanov ◽  
A. N. Irkitova ◽  
D. E. Dudnik
Keyword(s):  
No Value ◽  
Nutrient Medium ◽  
Bacillus Pumilus ◽  
Ph Value ◽  
Expiry Date ◽  
Maximum Value ◽  
Bacillus Genus ◽  
Number Of Bacteria ◽  
Bacillus Toyonensis ◽  
Selection Of

Abstract: This article aims to develop a probiotic for animals and aquaculture based on the Bacillus toyonensis B-13249 and Bacillus pumilus B-13250 strains. The selection of a nutrient medium was conducted for cultivating the inoculum of these microorganisms. Several bacteria fermentations of the Bacillus genus were performed in biological reactors with a capacity of 15 and 250 l. A technology for obtaining a finished probiotic for animals and aquaculture was developed. The results indicate that L-broth is the most optimal nutrient medium for cultivating the studied strains. The cultivation of B. toyonensis B-13249 and B. pumilus B-13250 strains in fermenters revealed that sporulation begins after 4–8 hours of fermentation. In contrast to the vegetative medium, the fermentative medium helped the bacilli develop a higher optical density (the maximum value in the B. pumilus strain – 2.400±0.149), pH value (maximum value in the B. toyonensis strain – 8.483±0.609) and titer (at least 1010 CFU/g). After 20–24 hours of incubation, both strains of bacilli in the fermenter, almost completely pass into endospores, which serve as a signal for the start of biomass centrifugation. This was indicated by the following: from a 15 l fermenter – 83.3±6.1 g of concentrate, from a 250 l fermenter – 499.8±51.4 g. The number of bacilli in a concentrated state was at least 1·1011 CFU/g for both strains. Obtaining a finished preparation required mixing bacterial concentrates with maltodextrin to a titer of at least 1·1010 CFU/g. The number of bacteria in the preparation checked every month during the year, recorded no value less than 1·1010 CFU/g. Thus, L-broth is most favorable for growing the mother culture of the B. toyonensis B-13249 and B. pumilus B-13250 strains, and fermentative nutrient medium – for the cultivation in fermenters. The expiry date of the bacilli-based biological preparation is at least 12 months, during which the drug’s polycomponence, color and consistency are preserved, in addition to the bacteria titer (at least 1·1010 CFU/g) and their viability.

scholarly journals Effect of pirimicarb application on field ppulations of Myzus persicae (Sulz.) (Homoptera: Aphididae) with different esterase levels

2000 ◽  
Vol 29 (4) ◽  
pp. 739-747
Author(s):  
Rui S. Furiatti ◽  
Sonia M. N. Lázzari
Keyword(s):  
Myzus Persicae ◽  
High Frequency ◽  
The Other ◽  
Biochemical Tests ◽  
Crop Season ◽  
High Frequencies ◽  
Potato Plants ◽  
Before And After ◽  
Potato Crops ◽  
Selection Of

The frequency and intensity of Myzus persicae (Sulz.) resistance were studied using bioassays and biochemical tests in two potato crops in Ibicoara, Bahia. Both areas received several insecticide applications which failed to control M. persicae. By the end of the crop season, one of the areas received two applications of pirimicarb and the other was not sprayed. Six samples of 700 aphids each were randomly collected before and after the pirimicarb applications, with an interval of four days. Specimens of M. persicae were also collected from isolated potato plants. At the laboratory, the samples were characterized by the CL50 based on insecticide bioassays and by total esterase activity using colorimetric assays. After the pirimicarb applications, the susceptible (S), the moderate resistant (R1) and mixed S/R1populations presented decreasing resistance frequencies (from 36.6 to 9.9; 12,0 to 7.5 and 11.4 to 5.9%, respectively). On the other hand, high resistant individuals (R2), extremely resistant (R3), and mixed populations of R1/R2 had increasing frequencies (from 17.7 to 36.7%; 2.3 to 9.1%, and 20.0 to 30.8%, respectively). The survival of S individuals was probably due to their wandering behavior on the plants to avoid sprayed areas. All changes in frequency were reflected in the LC50 and in the resistance ratio (rr). In the pirimicarb untreated area, high frequencies of R2, R3 and R1/R2 were observed. High frequency and resistance intensity of M. persicae in areas under intensive insecticide applications can be related to the selection of resistant populations and due to the entrance of winged migrants from spontaneous plants, where the frequency of R2+R3 was 81.4%.

scholarly journals Pedagogical heritage of the white émigré in the works of E. G. Osovsky

2020 ◽  
pp. 75-85
Author(s):  
Vera Petrovna Kirzhaeva ◽  
Alla Ivanovna Marinichenko ◽  
Alina Andreevna Piskunova
Keyword(s):  
Scientific Discourse ◽  
Comprehensive Analysis ◽  
Research Trends ◽  
Traditional Methods ◽  
New Methods ◽  
Methods Of Analysis ◽  
Extensive Coverage ◽  
The Subject ◽  
Selection Of ◽  
Pedagogical Science

The subject of this research is the works of the prominent Russian historian of education, corresponding member of the Russian Academy of Education E. G. Osovsky (1930-2004), dedicated to the analysis of schools, education and pedagogical thought of the white émigré. The article specifies the reasons for scholar’s selection of a new pedagogical topic at the turn of the 1980s – 1990s, outlines the implemented research approaches and problem fields, as well as offers the analysis of his central works. The presented in his works comprehensive analysis of the phenomenon of pedagogical emigration gave the opportunity for upcoming generations of researchers to create a holistic picture of establishment and development of schools, education and pedagogical thought of white émigré. The authors describe the key research trends of the scholar, characterize their impact upon the development of modern historical-pedagogical science and its prospects, which defines the relevance of this article. The extensive coverage of sources, introduction into the scientific discourse of the materials from personal archive of the scholar, rare publications comprise the scientific novelty of this study. The implementation of the approach based on the combination of traditional methods of historical and pedagogical science, as well as new methods of analysis (hermeneutical, contextual, microhistorical), provides the essential cross-disciplinarity and underlines the effectiveness of its application in studying the heritage of the prominent Russian pedagogues.

scholarly journals Problems of Managing Potato Plant Growth Under Micropropagation for Primary Seeds

2021 ◽  
Author(s):  
Sergey Kornatskiy
Keyword(s):  
Plant Growth ◽  
Nutrient Medium ◽  
Potato Plant ◽  
Root Formation ◽  
Basal Part ◽  
Stem Length ◽  
Chlorocholine Chloride ◽  
Single Node ◽  
Potato Plants ◽  
Murashige Skoog

This study shows the possibility of controlling the growth of potato microplants when adding the Murashige-Skoog (MS) nutrient medium chlorocholine chloride (CCC) to the composition at a concentration of 0.05-0.25 mg ⋅ L −1. Varietal specificity of the reaction of potato plants to the preparation was revealed. At optimal concentrations of CCC, plants with a stem length of 7-10 cm in 250 ml flasks were grown from single-node microcuttings for 1.5 months. Microplants were removed from the flasks using 20 cm scissors and unsterilized tweezers. The roots were shortened to 2-3 mm and the basal part of the stems was treated with a chalk-based paste containing 0.04% indolylbutyric acid (IBA). Plants were planted on 36 mm diameter Jiffy 7 peat pellets directly in the laboratory. The trays with the material were transferred to the greenhouse at the end of April at a temperature of 20-25 °C and placed in the conditions of the upper fine irrigation. After 3-4 days, mass root formation began in the plants, and another week later the seedlings were ready for use. By then the height of the plants was 10-15 cm, and the roots were clearly visible on the surface of the peat pellets. Planting these seedlings in a technological greenhouse to obtain minitubers was convenient and inexpensive. Keywords: potato, microcuttings, microtubers, minitubers, chlorocholine chloride, peat pellets

scholarly journals Virus Strain Discrimination Using Recombinant Antibodies

2000 ◽  
Vol 16 (1-2) ◽  
pp. 95-97 ◽  
Author(s):  
N. Boonham ◽  
I. Barker
Keyword(s):  
Monoclonal Antibodies ◽  
Polyclonal Antibodies ◽  
Recombinant Antibody ◽  
Plant Viruses ◽  
Antibody Fragments ◽  
Single Chain ◽  
Traditional Methods ◽  
Assay Sensitivity ◽  
Single Chain Fv ◽  
Selection Of

Most routine testing for plant viruses is currently carried out using monoclonal and polyclonal antibodies. Traditional methods of antibody production however can be time consuming and require the use of expensive cell culture facilities. Recombinant antibody technology however is starting to make an impact in this area, enabling the selection of antibody fragments in a few weeks compared with the many months associated with traditional methods and requires only basic microbiological facilities. Single chain Fv antibody fragments (scFv) have been selected from a synthetic phage-antibody library by affinity selection with purifiedPotato virus Y, ordinary strain (PVYO). The scFv selected was specific for PVY and detected 7 out of 9 isolates of PVYOwhilst it did not detect 15 isolates from the closely related necrotic strains PVYNand PVYNTN. In ELISA the scFv could be used to detect virus at concentrations of 50 ng/ml in plant sap and was shown to have similar limits of detection as commercially available PVY monoclonal antibodies. These results highlight the potential of the technology for the selection of strain specific antibodies with an affinity and assay sensitivity similar to traditional monoclonal antibodies and their use in viral diagnostics.

scholarly journals INTRODUCTION OF EXPLANTS AND REPRODUCTION ON NUTRIENT MEDIUM OF DONOR MATERIAL IN VITRO VARIETIES OF CALLISTEPHUS CHINENSIS (L.) NESS. FOR ITS FURTHER USE IN LANDSCAPING

2020 ◽  
Vol 1 (383) ◽  
pp. 89-96
Author(s):  
V. Polishchuk ◽  
S. Turchina ◽  
A. Balabak ◽  
I. Kozachenko ◽  
V. Mamchur ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Vegetative Propagation ◽  
Nutrient Medium ◽  
Cell Biology ◽  
Plant Biotechnology ◽  
Forest Steppe ◽  
Physical Parameters ◽  
Adaptation Period ◽  
Selection Of

The relevance of the research topic. On the recent methods of biotechnology are increasingly used in plant breeding and seed production. Herbaceous plants such as strawberries, potatoes, a vegetable, some medicinal and others are capable of vegetative propagation the traditional methods of culture, successfully introduced in both in vitro and can achieve a high rate of reproduction. Modern plant biotechnology – the sum of the technologies developed in molecular and cell biology of plants – a new stage in the development of the technology of plant breeding. With these improved characteristics may occur at the level of individual genes and individual genes that determine a specific trait, can be identified. They may be the final selection, they can be isolated, insert, delete, or modify the genotype or variety. Goal. Identify the features of the manifestation of economically valuable features and decorative properties of Callistephus chinensis and the inclusion of the best varieties in the biotech link, their adaptation to the conditions of the Forest-Steppe of Ukraine and their further use in landscaping. Methods. Laboratory – determination of seed germination; mathematical and statistical - for processing the reliability of the obtained research results. Results. The nutrient medium for growing plant tissues and cells, by analogy with the medium for culturing animal tissues, should contain all that the tissues in the plant organism receive from xylem and phloem currents of substances. However, in practice it has been found that vegetable juices cannot serve as a complete nutrient medium for growing isolated tissues and cells. This manifests the specificity of the receipt, transportation and especially the redistribution of nutrients in the plant. Based on the analysis, research was conducted to study the possibility of mass off-season vegetative propagation of plants of Callistephus chinensis in vitro. Practical recommendations on the selection of sterilizer, sterilization, nutrient medium and for the adaptation period of the best genotypes of this culture have been developed. As a result of the conducted researches the methods of selection of the initial plant material of Callistephus chinensis (Callistephus Chinensis (L.) NEES) and its surface sterilization, modification of existing aseptic culture methods have been studied and mastered. The morphogenetic potential of explants from different plant organs was investigated and selection of nutrient medium and study of the influence of plant growth regulators and physical parameters on the process of morphogenesis was carried out. The features of regeneration of isolated explants depending on the composition of the nutrient medium and selection of conditions for obtaining self-clones of Callistephus chinensis (Callistephus Chinensis (L.) NEES) were studied. Key words: in vitro, plant biotechnology, Callistephus Chinensis, nutrient medium, rhizogenesis.

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