scholarly journals Problems of Managing Potato Plant Growth Under Micropropagation for Primary Seeds

2021 ◽  
Author(s):  
Sergey Kornatskiy

This study shows the possibility of controlling the growth of potato microplants when adding the Murashige-Skoog (MS) nutrient medium chlorocholine chloride (CCC) to the composition at a concentration of 0.05-0.25 mg ⋅ L −1. Varietal specificity of the reaction of potato plants to the preparation was revealed. At optimal concentrations of CCC, plants with a stem length of 7-10 cm in 250 ml flasks were grown from single-node microcuttings for 1.5 months. Microplants were removed from the flasks using 20 cm scissors and unsterilized tweezers. The roots were shortened to 2-3 mm and the basal part of the stems was treated with a chalk-based paste containing 0.04% indolylbutyric acid (IBA). Plants were planted on 36 mm diameter Jiffy 7 peat pellets directly in the laboratory. The trays with the material were transferred to the greenhouse at the end of April at a temperature of 20-25 °C and placed in the conditions of the upper fine irrigation. After 3-4 days, mass root formation began in the plants, and another week later the seedlings were ready for use. By then the height of the plants was 10-15 cm, and the roots were clearly visible on the surface of the peat pellets. Planting these seedlings in a technological greenhouse to obtain minitubers was convenient and inexpensive. Keywords: potato, microcuttings, microtubers, minitubers, chlorocholine chloride, peat pellets

Author(s):  
E. A. Mukhamatdinova ◽  
I. S. Kovtun ◽  
M. V. Efimova

The microclones of potato (variety Lugovskoy) were grown on the modified Murashige-Skoog (MS) agar medium in the absence (control) or presence of JA at concentrations of 0.001, 0.1, and 10 µM. We evaluated plant growth parameters such as the length of the axial organs, the number of stolons, leaves, the area of the assimilating surface, and the wet and dry mass of aboveground and underground organs. For the first time, has been demonstrated, that jasmonic acid (0.1 and 10 µM) was showed a pronounced growth-stimulating effect on potato plants.


2003 ◽  
Vol 81 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Humberto Fabio Causin ◽  
Renata D Wulff

Morphological shade-avoidance responses have been hypothesized to be a form of adaptive plasticity to improve competition for light; however, little is known about their intraspecific variability and their effect on reproductive fitness. To compare plant responses either at a common age or at a common phenological stage, two experiments were conducted with early- and late-flowering Chenopodium album plants exposed to different red (660 nm) to far red (730 nm) ratios. In the first experiment, plant height and number of leaves were recorded at several times during the vegetative stage, and at the onset of flowering, each plant was harvested and other growth traits were measured. In the second experiment, three destructive harvests were performed across the whole plant cycle. Plant growth and development markedly differed between early- and late-flowering plants in all of the conditions tested. Light treatments significantly affected stem length, total leaf number, total leaf area, and relative allocation to leaf biomass. In all families, the response of stem elongation to light treatments decreased later in the development, while changes in the other plastic responses were mostly due to variations in plant growth. No significant treatment effect was found on relative biomass allocation to reproductive structures. However, individual seed mass significantly differed between certain groups, indicating that light quality can affect reproductive fitness through changes in traits other than fruit or seed set.Key words: Chenopodium album, fitness, intraspecific variability, phenotypic plasticity, red to far red ratio, shade-avoidance responses.


2020 ◽  
Vol 14 (2) ◽  
pp. 240-248
Author(s):  
Oscar Iván Monsalve ◽  
Eduardo María Espitia ◽  
Martha Marina Bolaños-Benavides

In potato crops in Colombia, fertilization has low efficiency in terms of absorption of nutrients by the plant due to fixing, leaching or volatilization processes. To counter this phenomenon, we evaluated the effect of the split application of fertilizers on potato plants and soil. Five treatments were evaluated: Control - fertilization used by farmers locally; As - fertilization recommended by the lab; AsSplit - monthly split of lab recommendation; AsSplit25 - monthly split of lab recommendation, reduced globally by 25%; AsSplit50 - monthly split of the lab recommendation, reduced globally by 50%. AsSplit treatment generated the highest yield (34.13 t ha-1), while treatments that reduced the amount of fertilizer by 25% and 50% obtained the lowest yield (30.94 and 29.57 t ha-1, respectively). However, they generated the lowest amount of NO3- in the leachate measurements at 30 and 90 cm deep. Our results suggest that designing the fertilization formula and applying it according to the requirements of the potato plant and soil fertility generates a positive effect on yield crop and environmental.


2008 ◽  
Vol 2 (1) ◽  
pp. 24-32
Author(s):  
Sh. H. AL-Obaidi ◽  
L. K. AL-Ani ◽  
W. A. Hussain

A field study were conducted to evaluate the efficacy of the entomopathogenic fungi Beauveria bassiana as abiocontrol agent for the cotton leaf worm Spodoptera littoralis infesting potato plants. Results indicated that soil treatment of the entomopathogenic fungi significantly reduced the percentage of plant infestation to 64.3, 60.7 % after 14, 21 days. Results of the infection intensity also significantly reduced in treatment of entomopathogenic fungi in soil to 18.7, 9.9 pore/leaf after 14, 21 days of treatment.The results revealed the higher efficiency of entomopathogenic fungi on plant yield, it become 633.3 gm in entomopathogenic fungi in soil treatment.


2017 ◽  
Vol 69 (1) ◽  
pp. 93-101
Author(s):  
Zexiong Chen ◽  
Juan Lou

Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.


Author(s):  
V. Semenchuk

Shows the results of research on the use of a biological product of Agat- 25K in potato crops. It has been established that spraying with the preparation of potato plants during the period of vegetation positively influenced their productivity. The increase in yield from the application of the preparation was 4.2 t/ha, which is 12%, the number of tubers from the 1st bush was 4.1 pieces. more than control, where spraying was not carried out.


Author(s):  
N. B. Kravets ◽  
M. Z. Mosula ◽  
N. M. Drobyk ◽  
N. V. Tulaidan ◽  
M. B. Cheterbok

Aim. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treat-ment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentra-tions of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators. Keywords: Carlina àcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, sprouts rooting.


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