scholarly journals Growth of Juveniles of the Freshwater Prawn Macrobrachium nipponense in the Laboratory, with Special Reference to the Estimation of Longevity for Wild Individuals

1990 ◽  
pp. 7-10 ◽  
Author(s):  
KAZUO MASHIKO
Keyword(s):  
Special Reference ◽  
Freshwater Prawn ◽  
Macrobrachium Nipponense

Pond culture of the freshwater prawn Macrobrachium nipponense in China.

2020 ◽  
Author(s):  
Yang Yi ◽  
Zong-wen Wu
Keyword(s):  
Crude Protein ◽  
Freshwater Prawn ◽  
Animal Origin ◽  
Macrobrachium Nipponense ◽  
The North ◽  
Food Of Animal Origin ◽  
Fresh Food ◽  
Earthen Ponds ◽  
One Year ◽  
Commercial Feeds

Abstract In China, conditioning of freshwater prawn (Macrobrachium nipponense) broodstock is conducted in earthen ponds at a density of 45-60 g m-2, at a ratio of 3-4 females to 1 male (Liu, 2004). Mixed feed and fresh food of animal origin are used for broodstock. Mating occurs when temperature rises above 18°C. Gravid prawn are stocked in cages at a density of 0.5 kg cage-1. After 20-25 days, prawn larvae hatch. Nursing is done in earthen ponds at a density of 2,000 prawns m-2. When the larvae grow to 0.8-1.0 cm long, the density should be reduced to 1,000 prawns m-2 or lower. After nursing for 20-30 days, when the prawns reach more than 1.0 cm, they can be stocked in grow-out systems. Two crops are possible in one year in south China and one crop in the north. Commercial feeds containing 35-40% crude protein are used to feed prawns.

Changes of protein-bound and free amino acids in the muscle of the freshwater prawn Macrobrachium nipponense in different salinities

Aquaculture ◽  
2004 ◽  
Vol 233 (1-4) ◽  
pp. 561-571 ◽  
Author(s):  
Wei-Na Wang ◽  
An-Li Wang ◽  
Lai Bao ◽  
Jian–Ping Wang ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Freshwater Prawn ◽  
Macrobrachium Nipponense

scholarly journals A newly identified Hippo homologue from the oriental river prawn Macrobrachium nipponense is involved in the antimicrobial immune response

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Ying Huang ◽  
Qian Ren
Keyword(s):  
Catalytic Domain ◽  
Control Cell ◽  
Size Control ◽  
Vital Role ◽  
Amino Acid Sequences ◽  
Freshwater Prawn ◽  
Macrobrachium Nipponense ◽  
Kinase Catalytic Domain ◽  
Oriental River Prawn ◽  
First Time

AbstractThe Hippo signalling pathway plays a vital role in organ size control, cell proliferation, apoptosis, and immune regulation. In this study, a Hippo homologue with three isoforms (named MnHippo-a, MnHippo-b, and MnHippo-c) was isolated and characterized for the first time from the freshwater prawn Macrobrachium nipponense. The deduced amino acid sequences of MnHippo-a (698 aa), MnHippo-b (688 aa), and MnHippo-c (656 aa) were highly similar, and they all contained an N-terminal S_TKc (serine/threonine protein kinase catalytic) domain and a C-terminal Mst1_SARAH (Sav/Rassf/Hpo) domain. MnHippo-a and MnHippo-c were derived from alternative splicing. Phylogenetic analysis was performed, and the results revealed that MnHippo was a member of the clade containing STPK4 and Hippo of Penaeus vannamei. The expression distribution showed that MnHippo was constitutively expressed in various tissues of uninfected prawns and highly expressed in the hepatopancreas and intestine. In prawns challenged with Vibrio parahaemolyticus and Staphylococcus aureus, the expression of MnHippo in haemocytes was significantly upregulated. Furthermore, in MnHippo-knockdown prawns injected with V. parahaemolyticus or S. aureus, the transcription levels of five antimicrobial peptides were downregulated. MnHippo silencing weakened the clearance of V. parahaemolyticus and S. aureus in prawns. The survival rate of the MnHippo-dsRNA group was obviously decreased from 2 to 6 days post-injection with V. parahaemolyticus or S. aureus. Hence, MnHippo might be involved in the antibacterial immune defence of M. nipponense.

The retinoid-X receptor gene from the freshwater prawn Macrobrachium nipponense: cloning, expression pattern and different responses of two splice variants during the molting cycle

Crustaceana ◽  
2013 ◽  
Vol 86 (13-14) ◽  
pp. 1586-1604 ◽  
Author(s):  
Huaishun Shen ◽  
Xin Zhou ◽  
Aixu Bai ◽  
Xiufang Ren
Keyword(s):  
Amino Acid ◽  
Developmental Stages ◽  
Splice Variants ◽  
Retinoid X Receptor ◽  
Full Length ◽  
Freshwater Prawn ◽  
Molting Cycle ◽  
Macrobrachium Nipponense ◽  
Acid Protein ◽  
Amino Acid Protein

The retinoid-X receptor (RXR) is among the most conserved members of the nuclear receptor superfamily and is widely studied in vertebrate and invertebrate families. RXR plays an important role in regulation of molting and/or metamorphosis, development and reproduction. We cloned the full-length cDNA sequence of the RXR from the freshwater prawn Macrobrachium nipponense (De Haan, 1849) (MnRXR) and investigated the expression profile of MnRXR in different developmental stages of embryos, in different tissues and in the molting cycle. Two MnRXR splice variants were identified: One, MnRXR-L, the full length of which was 2472 bp, encoded a 449-amino-acid protein; the second, MnRXR-S, the full length of which was 1832 bp, encoded a 420-amino-acid protein, in which the first 29 amino-acid residues of MnRXR-L were absent. MnRXR was observed in all developmental stages of embryos and had the highest expression level in the embryonised-zoea stage, it was highly expressed in hepatopancreas, gill and intestine among the ten tissues examined. The expression of MnRXR was rapidly up-regulated in the premolt stage and rapidly down-regulated after molting. Moreover, of the two MnRXR splice variants, only MnRXR-S was induced during the molting cycle, suggesting that the two splice variants play different roles in the molting cycle.

Artificial infection of the native Korean freshwater prawn Macrobrachium nipponense (De Haan, 1849) (Decapoda, Palaemonidae) with white spot syndrome virus (WSSV)

Crustaceana ◽  
2014 ◽  
Vol 87 (7) ◽  
pp. 866-880 ◽  
Author(s):  
J. M. Yun ◽  
B. S. Kim ◽  
S. M. Hwang ◽  
Y. B. Kim ◽  
W. B. Choi ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Freshwater Prawn ◽  
Post Inoculation ◽  
Macrobrachium Nipponense ◽  
Antennal Glands ◽  
Polymerase Chain ◽  
White Spot Syndrome ◽  
Dose Dependent

White spot syndrome virus (WSSV), an important shrimp pathogen that can affect both salt- and freshwater shrimp, has a high mortality rate that can reach up to 100% in 3-10 days. Therefore, we assessed the susceptibility of Macrobrachium nipponense (De Haan, 1849), a freshwater prawn native to Korea, to artificial WSSV infection. Although rearing in water containing WSSV-infected carcasses did not result in infection, M. nipponense was infected with WSSV after being fed a WSSV-containing diet, per os infection, or injection with the purified virus. In the feeding test, the first mortality was observed on the 7th day and cumulative mortality reached 30% by 3 weeks post-infection. After per os infection, experimental animals started dying on the second day and within 3 weeks mortality had reached 90%. In the injection test, the mortality was found to be dose-dependent. Prawn injected with 2.5 × 104, 900 and 80 copies/g body weight exhibited the first mortality at 1, 2 and 4 days post inoculation, with 100, 100 and 50% final mortality at 2, 10 and 12 days post inoculation, respectively. Histological observation revealed inclusion bodies in the gills, antennal glands and other tissues of ectodermal origin. Real-time polymerase chain reaction revealed the increase of viral titers, indicating viral replication in the inoculated host. This forms the first report of the experimental susceptibility of M. nipponense to WSSV.

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