scholarly journals RESEARCH NOTE PREVALENCE OF BOVINE TRYPANOSOME INFECTIONS IN DAMBOA LOCAL GOVERNMENT AREA BORNO STATE

2021 ◽  
Vol 21 ◽  
pp. 186-187
Author(s):  
M. I. Ahmed ◽  
T. I. O. Osiyemi ◽  
M. B. Ardo
Keyword(s):  
Local Government ◽  
Domestic Animals ◽  
Buffy Coat ◽  
Local Government Area ◽  
Tsetse Fly ◽  
Blood Collection ◽  
Limiting Factor ◽  
Blood Samples ◽  
Animal Trypanosomiasis ◽  
Dark Ground

African animal trypanosomiasis is a disease complex caused by pathogenic trypanosomes which are cyclically transmitted by tsetse-fly or mechanically by other biting flies to domestic animals. The disease has for long been a limiting factor to livestock production in tsetse-fly  infested regions of Africa (Anosa, 1983; Trail et al., 1985; Dwinger et al., 1986). It is now assuming significance in the tsetse-free vegetational zones (Nawathe et al., 1988) probably due to transhumance and ecological upset in favour of increasing insect population and activity in the area. The study was undertaken to ascertain the prevalence of bovine trypansome infection in the area and hoped that it will provide information on the epidemiology of the disease. A total of 151 blood samples were collected from 11 sedentary herds of cattle in Damboa Local Government Area between February to April, 1992. About 3ml of blood was collected from each animal inbijou bottle containing anticoagulant (Ethylene diamine tetra-acetic acid). The sex, age and breed of each animal were noted during blood collection. The blood samples were transported to the Laboratory on ice and examined using wet-film, stained thin smears, haematocrit centrifuge technique (Woo, 1969) and dark ground buffy coat. The packed cell volume was also recorded for each animal using Hawksley's haematocrit reader.

Intensity profile of lymphatic filariasis among community members of Yewa South Local Government Area, Ogun State, Nigeria

2021 ◽  
Vol 42 (1) ◽  
pp. 94-98
Author(s):  
F.F. Adekola ◽  
S. O Sam-Wobo ◽  
U.F. Ekpo ◽  
F. Oluwafemi ◽  
O.A. Surakat ◽  
...  
Keyword(s):  
Local Government ◽  
Lymphatic Filariasis ◽  
Age Groups ◽  
Local Government Area ◽  
Community Members ◽  
Chi Square ◽  
Blood Samples ◽  
Ogun State ◽  
Consistent Treatment ◽  
Study Participants

An epidemiological study was conducted in four communities of Yewa-South Local Government Area of Ogun State to determine the prevalence of lymphatic filariasis. Also investigated was the intensity among the study participants. Three hundred and sixty-two participants of both sexes and age ranging from 5 to 85 years were examined. Blood samples for parasitological examination were obtained from consenting participants between 21.00 and 02.00 a.m. The samples were prepared using thick blood smear technique. The data obtained were collected and analyzed using descriptive statistics and chi-square. The result showed that, from the 362 blood samples examined, 186 were positive with an overall of 51.4%. Microfilaraemic prevalence was not significantly related (p>0.05) by sex category with male having 78 (21.5%) and female 108 (29.3%). However, there was significant (p<0.05) relationship in the prevalence by age groups as Community members within the age group =50 had the highest prevalence of 52(14.4%) while the 20-29 years recorded the least prevalence of 18(5.0%). In terms of microfilaraemic load, all females and males who tested positive for lymphatic filariasis with 50.8% having light intensity of < 50mf/ml. There was no significant relationship between prevalence of lymphatic filariasis and communities studied (P>0.05). The high prevalence observed in these communities could be due to bushy vegetation. There is the important need for increased and consistent treatment with Albendazole and health education in all affected communitiesto reduce prevalence and possible elimination of the parasite. Keywords: Intensity, Lymphatic filariasis, prevalence, Ogun State, Nigeria

scholarly journals Species Composition of Sand-fly and Occurrence of Cutaneous Leishmaniasis in Owerri North Local Government Area, Imo State

2020 ◽  
pp. 1-5
Author(s):  
Ajero C M Ugoji ◽  
◽  
P N Amaechi A A ◽  
Chigbo U N ◽  
◽  
...  
Keyword(s):  
Public Health ◽  
Local Government ◽  
Species Composition ◽  
Cutaneous Leishmaniasis ◽  
Local Government Area ◽  
Laboratory Analysis ◽  
Sand Fly ◽  
Blood Samples ◽  
Imo State ◽  
Chi Square Analysis

Cutaneous leishmaniasis (CL) is a neglected tropical disease of public health value vectored by sand-fly in tropical and vegetative regions of the world. This study was carried out between March and September, 2019 to determine species composition of Sand fly and occurrence of cutaneous leishmaniasis in Owerri North Local Government Area of Imo State. Light and paper oil trap were used to trap 204 Sandflies from 3 villages and identified with standard entomological technique based on morphological characteristics. Assessment of the skin for cutaneous leishmaniasis manifestation was carried out on available respondents above 15 years. Blood samples were collected from 42 head of households and emptied into EDTA containers. Laboratory analysis of blood samples was carried out using standard method of staining and microscopy. Result was represented in percentages and subjected to chi-square analysis. The result revealed two sand-fly genera; Phlebotomus (135) and Lutzomyia (69) with abundant rates of 66.17% and 33.82% respectively. The different species were present in the villages sampled and female flies accounted for 45.59% of the collection. There was no typical CL skin manifestation observed among villagers, however, laboratory analysis of the household respondents showed 3(7.14) persons had amastigote form of leis hymenia in their blood. The study reveals occurrence of cutaneous leishmaniasis and its possible vectors in the study area. There is need for public health awareness, health education and application of effective control measures for sand-fly in this area

Xenomonitoring of trypanosome infectivity in Tse tse flies from Kagarko Local Government Area, Kaduna State Nigeria

2021 ◽  
Vol 42 (2) ◽  
pp. 213-219
Author(s):  
S.A. Mohammed ◽  
I.K. Auta ◽  
S.U. Adamu ◽  
N.J. Dadi-Mamud ◽  
B.A. Kugu
Keyword(s):  
Local Government ◽  
Local Government Area ◽  
Tsetse Fly ◽  
Tsetse Flies ◽  
Neglected Tropical Disease ◽  
Molecular Tools ◽  
Medical Importance ◽  
Antigen 5 ◽  
Biconical Traps ◽  
Trypanosoma Species

Trypanosomiasis is a re-emerging neglected tropical disease, afflicting domestic, wild animals and humans. In this study, the tsetse fly infectivity and species composition of Trypanosoma species were determined in Kagarko Local Government Area, Kaduna State Nigeria using entomological protocol. The tsetse flies were sampled using standard biconical traps and the flies collected were dissected for trypanosome using microscopy as well as expression of tsetse antigen 5 (TAg 5) using molecular tools. Forty seven (47) tsetse flies were collected over a period of four months, of which 13 (34.21%) were infected with Trypanosoma spp. The species of trypanosome identified include T. vivax 7(53.85%), T. congolense 5(38.46%) and T. brucei 1(7.69%), while expression of TAg 5 was observed in four of the examined flies. The presence of the flies with trypanosome of veterinary and medical importance  demonstrate the risk of transmission of trypanosomiasis in the study area.

scholarly journals Molecular identification of Trypanosoma brucei gambiense in naturally infected pigs, dogs and small ruminants confirms domestic animals as potential reservoirs for sleeping sickness in Chad

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 63
Author(s):  
Joël Vourchakbé ◽  
Zebaze Arnol Auvaker Tiofack ◽  
Tagueu Sartrien Kante ◽  
Mbida Mpoame ◽  
Gustave Simo
Keyword(s):  
Trypanosoma Brucei ◽  
Animal Species ◽  
Capillary Tube ◽  
Control Strategies ◽  
Small Ruminants ◽  
Domestic Animals ◽  
Buffy Coat ◽  
Blood Samples ◽  
Trypanosoma Brucei Gambiense ◽  
Potential Reservoir

Human African trypanosomiasis (HAT) has been targeted for zero transmission to humans by 2030. Animal reservoirs of gambiense-HAT could jeopardize these elimination goals. This study was undertaken to identify potential host reservoirs for Trypanosoma brucei gambiense by detecting its natural infections in domestic animals of Chadian HAT foci. Blood samples were collected from 267 goats, 181 sheep, 154 dogs, and 67 pigs. Rapid diagnostic test (RDT) and capillary tube centrifugation (CTC) were performed to search for trypanosomes. DNA was extracted from the buffy coat, and trypanosomes of the subgenus Trypanozoon as well as T. b. gambiense were identified by PCR. Of 669 blood samples, 19.4% were positive by RDT and 9.0% by CTC. PCR revealed 150 animals (22.4%) with trypanosomes belonging to Trypanozoon, including 18 (12%) T. b. gambiense. This trypanosome was found in all investigated animal species and all HAT foci. Between animal species or villages, no significant differences were observed in the number of animals harboring T. b. gambiense DNA. Pigs, dogs, sheep and goats appeared to be potential reservoir hosts for T. b. gambiense in Chad. The identification of T. b. gambiense in all animal species of all HAT foci suggests that these animals should be considered when designing new control strategies for sustainable elimination of HAT. Investigations aiming to decrypt their specific role in each epidemiological setting are important to achieve zero transmission of HAT.

Parasitological and molecular detection of Trypanosoma spp. in cattle, goats and sheep in Somalia

Parasitology ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. 1786-1791
Author(s):  
Ahmed A. Hassan-Kadle ◽  
Abdalla M. Ibrahim ◽  
Hamisi S. Nyingilili ◽  
Abdulkarim A. Yusuf ◽  
Rafael F. C. Vieira
Keyword(s):  
Internal Transcribed Spacer ◽  
Molecular Detection ◽  
Trypanosoma Evansi ◽  
Buffy Coat ◽  
Control Measures ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Animal Trypanosomiasis ◽  
And Control

AbstractAfrican animal trypanosomiasis (AAT) affects the livestock of 12.3 million Somalis and constrains their development and wellbeing. There is missing data on AAT in the country after the civil war of the 1990s. Therefore, this study has aimed to assess the prevalence of Trypanosoma spp. in 614 blood samples from cattle (n = 202), goats (n = 206) and sheep (n = 206) in Afgoye and Jowhar districts, Somalia using parasitological and molecular methods. Twenty-one out of 614 (3.4%; 95% CI: 2.1–5.2%) and 101/614 (16.4%; 95% CI: 13.6–19.6%) ruminants were positive for Trypanosoma spp. by buffy coat technique (BCT) and internal transcribed spacer 1 (ITS1)-polymerase chain reaction (PCR), respectively. Using ITS1-PCR, the highest prevalence was observed in cattle (23.8%; 95% CI: 18.4–30.1%) followed by goats (17.5%; 95% CI: 12.9–23.3%) and sheep (8.3%; 95% CI: 5.1–12.9%). A total of 74/101 (73.3%; 95% CI: 63.5–81.6%) ruminants were shown coinfection with at least two Trypanosome species. The four T. brucei-positive samples have tested negative for T. b. rhodesiense, by the human-serum-resistance-associated-PCR. Trypanosoma evansi, T. godfreyi, T. vivax, T. brucei, T. simiae and T. congolense were the Trypanosoma species found in this study. This is the first study on the molecular detection of Trypanosoma sp. in ruminants in Somalia. Further investigations and control measures are needed to manage Trypanosomiasis spreading in the country. Studies should also focus on the detection of T. b. rhodesiense in the country.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

1-Desamino-8-D-Arginine Vasopressin (DDAVP) Infusion in Type IIB von Willebrand’s Disease: Shortening of Bleeding Time and Induction of a Variable Pseudothrombocytopenia

1990 ◽  
Vol 64 (01) ◽  
pp. 117-120 ◽  
Author(s):  
Alessandra Casonato ◽  
M Teresa Sartori ◽  
Luigi de Marco ◽  
Antonio Girolami
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Count ◽  
Arginine Vasopressin ◽  
Calcium Concentration ◽  
Sodium Citrate ◽  
Bleeding Time ◽  
Blood Collection ◽  
Von Willebrand's Disease ◽  
Blood Samples ◽  
Von Willebrand’S Disease

SummaryWe have investigated the effects of 1-desamino-8-D-arginine vasopressin (DDAVP) infusion on platelet count and bleeding time in 4 patients with type IIB von Willebrand’s disease (vWd). Three of four patients showed a normalization of the bleeding time within 1 h after the infusion, while bleeding time was not modified in the fourth. In accordance with the literature, thrombocytopenia was observed after DDAVP infusion, but this thrombocytopenia was due to the anticoagulants used for blood collection. In two patients (F. I., G. F.) no thrombocytopenia was observed when platelets were counted by fingerstick method but there was a 20% platelet decrease in blood samples collected in sodium citrate and a 50% decrease in samples collected in EDTA. Dramatic falls in platelet counts (70–95%) were observed in the additional two patients (C. A., D.Z.) after DDAVP infusion, when both sodium citrate or EDTA were used as anticoagulants. In the latter two patients there was also a 50% decrease in platelet count when the fingerstick method was used. The decrease in the patient’s platelet count in EDTA samples after DDAVP infusion could be prevented, in part, by the previous additions of an anti GPIb monoclonal antibody and an anti GPIIb-IIIa monoclonal antibody.Thus, the thrombocytopenia observed in the four IIB vWd patients studied after DDAVP infusion seems to be, at least partially, a pseudothrombocytopenia depending on the calcium concentration in the blood samples and the availability of GPIb and GPIIb-IIIa receptors. These findings and the normalization of the bleeding time observed in three of the four patients has led us to reconsider the possible use of DDAVP in the treatment of our IIB vWd patients.

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