scholarly journals Prolactin reeeptor gene polymorphism and its association with litter size in Polish Landrace

2001 ◽  
Vol 44 (5) ◽  
pp. 547-552 ◽  
Author(s):  
M. Kmiec ◽  
A. Dybus ◽  
A. Terman
Keyword(s):  
Gene Locus ◽  
Prolactin Receptor ◽  
Reproductive Traits ◽  
Gene Marker ◽  
Chromosome 16 ◽  
Specific Primers ◽  
Dna Mutations ◽  
Pcr Rflp ◽  
Left And Right ◽  
Discrete Scale

Abstract. Prolactin reeeptor gene (PRLR), which is localized on chromosome 16 in pigs, is a candidate gene marker for reproductive traits. The experiment was aimed to detect the DNA mutations in that gene and to find probable relations between the genotype and some reproductive traits in Polish Landrace pigs 207 sows and 8 boars from pedigree herd were studied. The polymorphism in PRLR gene locus was detected using PCR-RFLP method, with specific primers and restriction enzyme Alul. Two different alleles were identified: PRLRA; PRLRB. The frequency of PRLRA was estimated at 0.44 and PRLRB – 0,56. In the studied population of pigs frequencies of genotypes AA, AB and BB were 0.247, 0.386 and 0.367, respectively. The relations between PRLR genotypes and teats number on the left and right body side, total number of teats, number of piglets in the litter and the age at the first parity were analyzed. All data were transformed from the discrete scale into the quantitative one. The analysis showed the significant dependencies (P ≤ 0,01) between the number of piglets in the first litter and genotype of prolactin receptor. Significantly greater number of piglets characterized the sows carrying AA genotype.

scholarly journals PROLACTIN RECEPTOR GENE (PRLR) ROLE IN SWINE REPRODUCTION

2019 ◽  
Vol 17 (1) ◽  
pp. 75-80
Author(s):  
Zh. Sabev
Keyword(s):  
Receptor Gene ◽  
Prolactin Receptor ◽  
Reproductive Traits ◽  
Biological Functions ◽  
Chromosome 16 ◽  
Porcine Chromosome ◽  
Genotype Frequencies ◽  
Litter Traits ◽  
Pcr Rflp ◽  
Prolactin Receptor Gene

PRLR gene has been studied as a candidate gene for litter size in swine because of prolactin biological functions and their association with reproduction. Prolactin receptor gene is mapped on porcine chromosome 16. The established PCR-RFLP polymorphism at PRLR locus demonstrated the presence of two alleles, А and В and three genotypes АА, АВ and ВВ. There were found different allele and genotype frequencies in different pig populations with variation of the effects of PRLR genotypes on litter traits. Additional studies should be done in specific breeds and populations to evaluate the association of existing PRLR locus polymorphism with reproductive traits before application of marker-assisted selection in these populations.

scholarly journals Effects of BF Gene Polymorphism on the Reproductive Performance of Hybrid Polish Landrace X Polish Large White Sows

2016 ◽  
Vol 66 (1) ◽  
pp. 125-131
Author(s):  
Dorota Napierała ◽  
Maria Kawęcka ◽  
Eugenia Jacyno ◽  
Beata Matysiak ◽  
Anita Kołodziej-Skalska
Keyword(s):  
Gene Polymorphism ◽  
Litter Size ◽  
Reproductive Performance ◽  
Reproductive Traits ◽  
Specific Primers ◽  
Large White ◽  
Potential Relationship ◽  
Pcr Rflp

AbstractThe aim of this study was to determine the potential relationship between variants of the BF gene and litter size in Polish Landrace x Polish Large White sows. To identify polymorphism within the BF gene, the PCR-RFLP method was applied, using specific primers and the SmaI enzyme. The researchers identified the presence of two alleles, T and C, with frequencies: 0.68 and 0.32, giving three genotypes with frequencies: 0.49, 0.37 and 0.14, respectively for TT, TC and CC. Analysis of relationships between the various genotypes of the BF gene and selected reproductive traits showed significant differences. Sows with TT genotype in the BF locus, gave birth to significantly more live piglets in the first litter (10.24) compared with sows with the CC genotype (7.13).

scholarly journals Porcine prolactin receptor genotypes and production and reproduction traits in Hungarian Large White and Landrace sows (Brief Report)

2010 ◽  
Vol 53 (4) ◽  
pp. 497-499
Author(s):  
K. Kovacs ◽  
L. Fesus ◽  
A. Zsolnai ◽  
A. Nyiri ◽  
I. Anton
Keyword(s):  
Litter Size ◽  
Receptor Gene ◽  
Mammalian Species ◽  
Prolactin Receptor ◽  
Reproductive Traits ◽  
Pituitary Hormone ◽  
Chromosome 16 ◽  
Large White ◽  
Anterior Pituitary Hormone ◽  
Reproduction Traits

Abstract. Prolactin is an anterior pituitary hormone involved in many endocrine activities and plays and essential role in reproduction. Its receptor (PRLR) was detected in various tissues including brain, ovary, placenta, an uterus in several mammalian species (BOLE-FEYSOT et al. 1998). Reproductive performance of sows is a crutial point in pig production with significant economic importance and may be estimated with the help of markers. Porcine prolactin receptor gene is said to be a candidate genetic marker for reproductive traits. It has been mapped to porcine chromosome 16 (Vincent et al. 1997). There is a C/G SNP in PRLR gene (KMIEC et al. 2001) at the position of 203 (GAN: U96306) which eliminates an AluI cleavage site. The effect of this polymorphism on litter size in various breeds has been estimated (VINCENT et al. 1998, ROTHSCHILD et al. 1998, VAN RENS et al. 2002, KMIEC and TERMAN, 2004, DRÖGEMÜLLER et al. 2001, KORWIN-KOSSAKOWSKA et al. 2003). The influence of the bovine hormone variant was also estimated (RATNA-KUMARI et al. 2008). The aim of the study was to estimate PRLR AluI polymorphism effects on litter size in Hungarian Large White (HLW) and Hungarian Landrace (HL) breeds.

scholarly journals The Association of an SNP in the EXOC4 Gene and Reproductive Traits Suggests Its Use as a Breeding Marker in Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Yingting He ◽  
Xiaofeng Zhou ◽  
Rongrong Zheng ◽  
Yao Jiang ◽  
Zhixiang Yao ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Fragment Length Polymorphism ◽  
Regulatory Element ◽  
Reproductive Traits ◽  
Specific Sequence ◽  
Litter Weight ◽  
Potential Binding ◽  
Pcr Rflp ◽  
Teat Number ◽  
Myeloid Zinc Finger

In mammals, the exocyst complex component 4 (EXOC4) gene has often been reported to be involved in vesicle transport. The SNP rs81471943 (C/T) is located in the intron of porcine EXOC4, while six quantitative trait loci (QTL) within 5–10 Mb around EXOC4 are associated with ovary weight, teat number, total offspring born alive, and corpus luteum number. However, the molecular mechanisms between EXOC4 and the reproductive performance of pigs remains to be elucidated. In this study, rs81471943 was genotyped from a total of 994 Duroc sows, and the genotype and allele frequency of SNP rs81471943 (C/T) were statistically analyzed. Then, the associations between SNP rs81471943 and four reproductive traits, including number of piglets born alive (NBA), litter weight at birth (LWB), number of piglets weaned (NW), and litter weight at weaning (LWW), were determined. Sanger sequencing and PCR restriction fragment length polymorphism (PCR-RFLP) were utilized to identify the rs81471943 genotype. We found that the genotype frequency of CC was significantly higher than that of CT and TT, and CC was the most frequent genotype for NBA, LWB, NW, and LWW. Moreover, 5′-deletion and luciferase assays identified a positive transcription regulatory element in the EXOC4 promoter. After exploring the EXOC4 promoter, SNP −1781G/A linked with SNP rs81471943 (C/T) were identified by analysis of the transcription activity of the haplotypes, and SNP −1781 G/A may influence the potential binding of P53, E26 transformation specific sequence -like 1 transcription factor (ELK1), and myeloid zinc finger 1 (MZF1). These findings provide useful information for identifying a molecular marker of EXOC4-assisted selection in pig breeding.

scholarly journals OPN gene locus is associated with the risk of knee osteoarthritis: a case–control study

2019 ◽  
Vol 39 (2) ◽  
Author(s):  
Houlai Shang ◽  
Yuedong Hao ◽  
Wenhao Hu ◽  
Xiaohui Hu ◽  
Qing Jin
Keyword(s):  
Knee Osteoarthritis ◽  
Chinese Population ◽  
Gene Locus ◽  
Case Control Study ◽  
Fragment Length Polymorphism ◽  
Case Control ◽  
Chinese Han ◽  
Knee Oa ◽  
Pcr Rflp ◽  
Control Study

AbstractBackground/aims: Studies have demonstrated that osteopontin (OPN) was associated with the severity and development of knee osteoarthritis (OA). Methods: The purpose of this case–control study was to investigate the association between OPN gene rs11730582 polymorphism and knee OA risk in a Chinese population. Genotyping was analyzed using standard PCR and restriction fragment length polymorphism (PCR-RFLP). Results: The present study found that C allele or CC genotype of OPN gene rs11730582 polymorphism was related to decreased risk for knee OA. Furthermore, positive associations were obtained amongst the females, and body mass index (BMI) < 25 kg/m2 groups. Conclusions: To sum up, the present study reveals that OPN gene rs11730582 polymorphism decreases the risk of knee OA in Chinese Han population.

scholarly journals Association of RBP4 Genotype with Phenotypic Reproductive Traits of Sows

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
A. Marantidis ◽  
G. P. Laliotis ◽  
M. Avdi
Keyword(s):  
Litter Size ◽  
Negative Impact ◽  
Reproductive Traits ◽  
Retinol Binding Protein ◽  
Rapid Improvement ◽  
Retinol Binding Protein 4 ◽  
Reproductive Characteristics ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Rbp4 Gene

PCR-RFLP was applied to a commercial crossbred pig population in order to investigate the association between polymorphism (SNP) of Retinol-binding protein 4 (RBP4) gene and reproductive performance. 400 sows were genotyped and 2000 records of reproductive traits were used in order to retrieve information about the allele frequencies and the association of the RBP4 gene with main reproductive characteristics of the population. A deviation from the Hardy-Weinberg equilibrium was observed as a result of the AB genotype excess. In addition, the AA genotype saw statistically significant higher values of (i) the total number of born piglets (p<0.05), (ii) the number of piglets born alive (p<0.01), and (iii) the number of weaned piglets (p<0.01). The number of the mummified piglets and the number of the piglets born dead did not differ between the various RBP4 genotypes. Interestingly, the AA genotype had a negative impact (p<0.05) on the number of piglets born dead, resulting indirectly in a larger litter size. In conclusion, the AA genotype and in extension the A allele of RBP4 gene are in favor of producing larger litter size, suggesting that the RBP4 gene may be used in Marker-Assisted Selection (MAS) programs for a rapid improvement of the reproductive characteristics in pigs.

scholarly journals Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs

2015 ◽  
Vol 58 (2) ◽  
pp. 317-323 ◽  
Author(s):  
T. Kumchoo ◽  
S. Mekchay
Keyword(s):  
Litter Size ◽  
Reproductive Traits ◽  
Snp Markers ◽  
Strong Linkage Disequilibrium ◽  
Nucleotide Polymorphisms ◽  
Large White ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Acid Exchange

Abstract. Osteopontin (OPN) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in commercial Thai Large White pigs. A total of 320 Thai Large White sows were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A, c.573T> C and c.881C> T revealed amino acid exchange rates of p.110Ala> Thr, p.159Val> Ala and p.262Pro> Ser, respectively, and were then segregated. These three SNPs were significantly associated with total number born (TNB) and number born alive (NBA) traits. No polymorphisms of the two SNP markers (c.278A> G and c.452T> G) were observed in this study. Moreover, the SNPs at c.425G> A and c.573T> C were found to be in strong linkage disequilibrium. The association of OPN with litter size emphasizes the importance of porcine OPN as a candidate gene for reproductive traits in pig breeding.

Prolactin receptor maps to pig Chromosome 16

1997 ◽  
Vol 8 (10) ◽  
pp. 793-794 ◽  
Author(s):  
A.L. Vincent ◽  
L. Wang ◽  
C.K. Tuggle ◽  
A. Robic ◽  
M.F. Rothschild
Keyword(s):  
Prolactin Receptor ◽  
Chromosome 16

PCR-based molecular discrimination betweenMaritrema eroliaeandProbolocoryphe uca(Digenea: Microphallidae) in Kuwait Bay

2013 ◽  
Vol 88 (2) ◽  
pp. 177-182
Author(s):  
W.Y. Al-Kandari ◽  
S.A. Al-Bustan ◽  
M. Alnaqeeb ◽  
A.M. Isaac
Keyword(s):  
Fragment Length Polymorphism ◽  
Pcr Amplification ◽  
Direct Pcr ◽  
Specific Primers ◽  
Kuwait Bay ◽  
Future Studies ◽  
Larval Stages ◽  
Marine Snails ◽  
Pcr Rflp ◽  
Species Specific

AbstractMicrophallid trematodes are common parasites in marine snails and crustacean hosts at Kuwait Bay. The larval stages of two microphallids,Maritrema eroliaeandProbolocoryphe uca, are difficult to differentiate morphologically. In this study, two PCR-based techniques were established for quick and accurate discrimination between the larval stages of the two microphallid species, employing restriction fragment length polymorphism (PCR-RFLP) and species-specific primers. Both techniques utilized nucleotide differences in the second internal transcribed region (ITS2) of the ribosomal DNA (rDNA) in the two species. For the PCR-RFLP technique, restriction enzymeAvaII was selected and it generated different restriction profiles among the two microphallids. In addition, species-specific primers were prepared for each microphallid species that amplified distinctive fragments. Both techniques showed that the larval stages of the two microphallid species can be identified accurately. However, direct PCR amplification using species-specific primers was more advantageous than the PCR-RFLP technique since it allowed rapid and specific discrimination between the two species. This technique provides a useful tool that can be used in future studies for the study of the distribution of microphallid species and their definitive hosts at different localities of Kuwait Bay.

Identification of Medicinal Ganoderma Species Based on PCR with Specific Primers and PCR-RFLP

Planta Medica ◽  
2008 ◽  
Vol 74 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Zhou Xuanwei ◽  
Li Qizhang ◽  
Yin Yizhou ◽  
Chen Yiyuan ◽  
Lin Juan
Keyword(s):  
Specific Primers ◽  
Pcr Rflp
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