scholarly journals Developmental changes of <i>GHR</i> and <i>IGF-I</i> mRNA expression in lamb rumen

2012 ◽  
Vol 55 (1) ◽  
pp. 72-77
Author(s):  
S. Cheng ◽  
F. Li ◽  
J. Guo ◽  
J. Pei ◽  
Y. Ma ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Growth Hormone Receptor ◽  
Developmental Changes ◽  
Expression Levels ◽  
Real Time Quantitative Pcr ◽  
Igf I ◽  
Pcr Method ◽  
Dorsal Sac ◽  
Change Tendency ◽  
Gene Expression Levels

Abstract. Lambs from birth (0-day-old) to 56-day-old were selected in present study to investigate developmental changes of growth hormone receptor (GHR) and insulin-like growth factor I (IGF-I) mRNA expression in their rumen tissue. Forty-five lambs (5 lambs per group) were slaughtered at 0, 7, 14, 21, 28, 35, 42, 49, 56 days of age respectively for sampling the tissue of the rumen dorsal sac. The abundance of GHR and IGF-I mRNA were detected through real-time quantitative PCR method. The results indicated that the expression levels of GHR and IGF-I mRNA had similar change tendency in rumen tissue that the GHR and IGF-I mRNA abundance decreased with age from birth to 56-day-old. There was significant positive correlation between the two gene mRNA expression levels. The results suggested that GHR and IGF-I gene expression levels had the specific developmental pattern in rumen tissue.

Correlation of messenger RNA expression patterns of ERCC1, TS, EGFR, and VEGFR2 with KRAS and BRAF mutational status in advanced colorectal cancer: Implications for targeted therapies.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 383-383
Author(s):  
Martin K. H. Maus ◽  
Craig Stephens ◽  
Stephanie H. Astrow ◽  
Peter Philipp Grimminger ◽  
Dongyun Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Mrna Expression ◽  
Advanced Colorectal Cancer ◽  
Expression Patterns ◽  
Mrna Levels ◽  
Expression Levels ◽  
Mutational Status ◽  
Mrna Expression Levels ◽  
Gene Expression Levels

383 Background: Gene expression levels of ERCC1, TS, EGFR and VEGFR2 may have predictive value for the personalized use of standard chemotherapeutics as well as agents targeting the EGFR and VEGF pathways and the efficacy of EGFR directed monoclonal antibodies like panitumumab and cetuximab has been confirmed to be dependent on wt KRAS and wt BRAF in patients with advanced colorectal cancer. We investigated the correlations between KRAS/BRAF mutational status and the mRNA expression levels of these genes. Methods: Formalin-fixed paraffin-embedded tumor specimens from 600 patients with advanced colorectal adenocarcinoma were microdissected and DNA and RNA was extracted. Specifically designed primers and probes were used to detect 7 different base substitutions in codon 12 and 13 of KRAS, V600E mutations in BRAF and the expression levels of ERCC1, TS, EGFR and VEGFR2 by RT-PCR. Results: Mt KRAS tumors had significantly lower TS and EGFR gene expression levels compared with wt KRAS (p<0,001), whereas mt BRAF tumors showed significantly increased TS and EGFR mRNA levels compared to wt BRAF (p<0,001). Mt BRAF tumors showed significantly higher mRNA levels than mt KRAS tumors (p<0,001). ERCC1 and VEGFR2 mRNA levels were significantly down-regulated in mt KRAS specimen (p<0,001), but showed no significant correlation with BRAF mutational status. Conclusions: KRAS and BRAF mutations are associated with opposite mRNA expression levels for TS and EGFR. Recently, resistance to BRAF inhibition in mt BRAF colorectal tumors has been shown in preclinical models to be associated with up-regulation of EGFR. Our data suggests that BRAF mutants are associated with high EGFR levels at the time of diagnosis, and not necessarily part of an acquired mechanism of resistance. Significantly lower mRNA expression levels of VEGFR2 in mt KRAS tumors may explain lower response to angiogenesis inhibition seen in the TML study.

scholarly journals Central and peripheral effects of chronic food restriction and weight restoration in the rat

2009 ◽  
Vol 296 (2) ◽  
pp. E282-E290 ◽  
Author(s):  
Kimberly P. Kinzig ◽  
Sara L. Hargrave ◽  
Erin E. Tao
Keyword(s):  
Mrna Expression ◽  
Food Restriction ◽  
Human Subjects ◽  
Dorsomedial Hypothalamus ◽  
Expression Levels ◽  
Weight Restoration ◽  
Agouti Related Protein ◽  
Chronic Food Restriction ◽  
Gene Expression Levels

Previous studies have demonstrated that some endocrine consequences of long-term caloric restriction persist after weight restoration in human subjects. Here we evaluate effects of chronic food restriction in rats that were restricted to 70% of control kcal for 4 wk and subsequently weight restored. Measures were taken from rats at 80% (chronically restricted; CR), 90% (partially weight restored; PR), 100% (fully weight restored; FR), and after 4 wk at 100% body weight of controls (extended weight restored; ER). Plasma insulin and leptin were decreased, and ghrelin was increased in CR compared with controls. Leptin and ghrelin normalized with weight restoration at PR, FR, and ER; however, baseline insulin was not normalized until the ER state. Hypothalamic mRNA expression levels for proopiomelanocortin (POMC), agouti-related protein (AgRP), and neuropeptide Y (NPY) revealed significantly less POMC mRNA expression in CR and PR rats, and significantly less arcuate NPY mRNA in PR and FR. In the dorsomedial hypothalamus, CR, PR, and FR rats had significantly increased NPY expression that was not normalized until the ER state. In response to a test meal, insulin and ghrelin release patterns were altered through the FR stage, and ghrelin remained affected at ER. Collectively, these data demonstrate that mere weight restoration is not sufficient to normalize hypothalamic gene expression levels and endocrine responses to a meal, and that meal-related ghrelin responses persist despite weight restoration for up to 4 wk.

Epidermal growth factor receptor (EGFR) mRNA expression levels are strongly correlated between primary colorectal cancer and corresponding liver metastases

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 4106-4106
Author(s):  
H. Kuramochi ◽  
K. Hayashi ◽  
K. Uchida ◽  
M. Yamamoto ◽  
K. D. Danenberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Metastases ◽  
Mrna Expression ◽  
Growth Factor Receptor ◽  
Primary Cancer ◽  
Egfr Gene ◽  
Expression Levels ◽  
Epidermal Growth ◽  
Mrna Expression Levels ◽  
Gene Expression Levels

4106 Background: As it has been well known that epidermal growth factor receptor (EGFR) is strongly related to a tumor proliferation and a metastasis, new EGFR-inhibitory small molecules, such as gefitinib and erlotinib, and EGFR antibodies have been developed recently. It is reported that the high expression levels of EGFR mRNA are associated with high response probability and longer progression-free survival in gefitinib-treated lung cancer patients. Thus, since liver metastases are the main cause of death for most of colorectal cancer (CRC) patients, it is reasonable to expect that measurement of EGFR gene expression levels in liver metastases would provide the best prediction of therapy benefit, but in most cases, only biopsies of the patient’s primary tumor are readily available for analysis. Our aim was to determine how EGFR gene expression levels in primary CRC were related to those in liver metastases. Methods: Thirty-one pairs of primary CRC and corresponding liver metastases were analyzed. (18 males and 13 females: Median age 66 (range 45–85)). Formalin-fixed, paraffin-embedded tumor specimens were dissected by using laser-captured microdissection. RNA was extracted and cDNA was prepared by reverse-transcription. Quantitation of target gene and internal reference gene was performed using real-time PCR (Taqman PCR). Results: No significant difference was seen between median mRNA expression levels of EGFR in primary cancer and those in corresponding liver metastases (Median value: 1.35 vs 1.24, p=0.99 , Wilcoxon signed rank test), although the median value of EGFR mRNA from normal liver tissue is significantly higher than those from normal colon mucosa (Median value: 5.06 vs 1.39, p<0.0001). When matched tissue sets were compared on an individual basis, there was a significant correlation for EGFR mRNA expression between primary cancer and corresponding liver metastases (rs=0.78, p<0.0001, Spearman rank correlation coefficient). Conclusions: A good prediction of EGFR mRNA levels in liver metastases can be obtained by measuring those of primary CRC. No significant financial relationships to disclose.

scholarly journals chHDAC11 mRNA Expression During Prenatal and Postnatal Chicken (Gallus gallus) Brain Development

2022 ◽  
Vol 8 (1) ◽  
pp. 17-25
Author(s):  
Seyedeh Rezvaneh Moadabpour ◽  
◽  
Afsaneh Shokri ◽  
Farhad Mashayekhi ◽  
Mohammad Mehdi Sohani ◽  
...  
Keyword(s):  
Brain Development ◽  
Mrna Expression ◽  
Cell Cycle Progression ◽  
Human Subjects ◽  
Statistical Significance ◽  
Histone Deacetylation ◽  
Real Time Quantitative Pcr ◽  
Pcr Method ◽  
Conserved Gene ◽  
Regulation Of Cell Cycle

Background: Histone deacetylation plays an essential role in transcriptional regulation of cell cycle progression and other evolutionary processes. Several results confirm the importance of the latest found HDAC11 gene to deacetylate histone core in neurons and their supportive cells in developing the vertebrate Central Nervous System (CNS). Objectives: This study investigates the HDAC11 potential role in early chicken CNS development by studying its mRNA expression profile which may have unique means in studying human subjects. Materials & Methods: Chicken HDAC11 RNAs were reverse transcribed to cDNAs, and the amount of chHDAC11 transcripts was measured by ΔCT mean calculation using the real-time quantitative PCR method. One-way ANOVA and Duncan’s analysis (SigmaStat software version 4.0) were used to test the statistical significance of the results. The levels of significance were set at P≤0.05. Quantitative data are presented as Mean±SD. Results: The amount of HDAC11 mRNAs gradually increases, at least 2-3 times, from as early as day 14 (E14/HH40) of prenatal cortex formation to day P0 (E20=HH45) and continue to increase to day 40 in both cortical and hippocampal regions of the postnatal chicken brain during development (*P≤0.05). HDAC11 mRNA is not only expressed in the postnatal cortex and hippocampi regions but also—for the first time—in the developing brain during the prenatal period. Conclusion: Our results show a possibly important role for the latest found HDAC11 conserved gene in the development of vertebrates’ embryonic brain, which in turn may have a significant impact on understanding human brain development.

scholarly journals JNK Signaling Pathway Suppresses LPS-Mediated Apoptosis of HK-2 Cells by Upregulating NGAL

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Mei Han ◽  
Yuxia Pan ◽  
Mengying Gao ◽  
Junli Zhang ◽  
Fan Wang
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Signaling Pathway ◽  
Caspase 3 ◽  
Cell Injury ◽  
Jnk Signaling ◽  
Expression Levels ◽  
Jnk Signaling Pathway ◽  
Renal Tubular ◽  
Gene Expression Levels

Objective. To explore the role of the c-Jun N-terminal kinase (JNK) signaling pathway in upregulated NGAL expression and its antiapoptotic mechanism in lipopolysaccharide (LPS)-mediated renal tubular epithelial cell injury. Methods. In vitro, HK-2 cells were divided into five groups (Con, LPS 1 h, LPS 3 h, LPS 6 h, and LPS 12 h groups) based on the time of LPS (10 μM) treatment. NGAL and caspase-3 gene expression levels were detected by RT-PCR to assess dynamic changes. HK-2 cells were pretreated with SP600125 (20 μM) for 2 hours, followed by LPS (10 μM) stimulation for 3 hours. NGAL and caspase-3 gene expression levels were then determined. Results. NGAL mRNA was increased significantly within 6 hours, and caspase-3 mRNA was increased within 3 hours after treatment (P<0.05). Correlation analysis showed a high correlation between their expression (r = 0.448, P<0.05). After pretreatment with SP600125, mRNA expression of NGAL in the LPS group was inhibited, while that of caspase-3 was increased significantly. The NGAL mRNA expression level in the SB + LPS group was decreased significantly compared with that in the LPS group, but it was slightly higher than that in the SP group (∼1.5 times of that in the Con group). However, caspase-3 mRNA expression was increased significantly in the SB + LPS group (P<0.001) (3.5 times of that in the Con group). It also showed a significant increase compared with SP and LPS groups (P<0.001 vs. SB group; P<0.05 vs. LPS group). We also found that NGAL and caspase 3 proteins were increased significantly in LPS and SP + LPS groups, but SP600125 decreased the NGAL level by almost 35% and increased the caspase 3 level by 50% in the SP + LPS group compared with the LPS group (P<0.05). Conclusions. The JNK signaling pathway inhibits LPS-mediated apoptosis of renal tubular epithelial cells by upregulating NGAL.

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