scholarly journals Evaluation of sperm motility and velocity of different genetic groups of bucks using Computer-Assisted Sperm Analyzer

2020 ◽  
Vol 48 (2) ◽  
pp. 67-74
Author(s):  
MR Islam ◽  
MKU Talukder ◽  
MS Rahman ◽  
SS Husain
Keyword(s):  
Semen Quality ◽  
Computer Assisted ◽  
Scrotal Circumference ◽  
Future Performance ◽  
Genetic Groups ◽  
Straight Line ◽  
Semen Volume ◽  
Path Distance ◽  
Positive Correlations ◽  
Better Than

Semen quality assessment is a prime prerequisite for any breeding programme. Computer-Assisted Sperm Analyzer (CASA) can measure the semen quality very accurately and sharply. The research was conducted at American Dairy Limited (ADL) at Vangnahati, Sreepur, Gazipur. A total of 120 ejaculates were collected from three genetic groups of bucks namely Black Bengal (BB), Boer and Jamunapari (JP) with a view to characterize and compare the semen motility and velocity parameters using Computer- Assisted Sperm Analyser. Significantly (p<0.05) higher ejaculate volume was found in Boer buck (1.73±0.16 ml) with the lower in BB buck (0.98±0.14), respectively. But significantly (p<0.05) higher total, forward progressive and fast motility was found in BB (55.47±3.17, 47.23±2.74 and 37.62±2.40%) and lower in JP (40.67±3.37, 30.97±2.91 and 21.81±2.55%), respectively. Significantly (p<0.01) fastest straight line and average path distance was observed in Boer bucks (12.29±1.63 and 15.89±1.95 μm) than Black Bengal and Jamunapari bucks (4.38±1.19 and 7.26±1.57; and 3.14±1.27 and 8.42±1.95 μm), respectively. The distance curved line (DCL) and distance straight line (DSL) was significantly (p<0.05) higher in Boer (29.13±4.87, 12.29±1.63 and 15.89±1.95 _m/sec) and lower in BB (22.07±4.36 and 7.26±1.57 _m/sec), respectively. In case of curvilinear velocity, straight line velocity and average path velocity value was significantly (p<0.01) higher in Boer (127.08±13.88, 50.01±7.68 and 68.13±7.25 _m/sec) with lower in JP (62.01±12.77, 15.75±7.35 and 22.39±9.42) where as linearity and straightness was higher in Boer and lower in BB (p>0.05), respectively. The scrotal circumference had positive correlations with semen volume and curvilinear velocity for all the genotypes. It was concluded that semen of Boer bucks found consistently better than other two genetic groups of bucks. Further, competitive studies with large sample may be performed to evaluate the breed specific differences in available goat breeds. I think this study also disclosed the some rare parameters of semen quality by using Computer-Assisted Sperm Analyzer which was impossible in manually. These results of the present study could be an authentic guideline in future performance tests of different genetic groups of bucks. Bang. J. Anim. Sci. 2019. 48 (2): 67-74

scholarly journals Frozen semen quality and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls in Bangladesh

2020 ◽  
Vol 5 (1) ◽  
pp. 33-41
Author(s):  
Md Mehedi Hasan ◽  
Md Rafikul Islam ◽  
Syed Sakhawat Husain ◽  
Auvijit Saha Apu
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Computer Assisted ◽  
Holstein Friesian ◽  
Frozen Semen ◽  
Semen Volume ◽  
Before And After ◽  
Immotile Spermatozoa ◽  
Better Than

The present study was conducted to evaluate the frozen semen quality in terms of different motility patterns and fertility of imported pure Holstein Friesian and Sahiwal breeding bulls maintained under farming condition of Bangladesh. 100% pure Holstein Friesian and Sahiwal breeding bulls were imported and thereafter reared in the American dairy limited (ADL). Semen samples were collected once a week, processed and frozen in the laboratory of ADL. On the day of collection, semen volume and sperm concentration measured immediately after collection. Different motility patterns of spermatozoa (progressive, fast, slow, local, immotile) were measured by Computer Assisted Semen Analyzer (CASA). Effect of breed was found significant (p<0.05) on semen volume and sperm concentration. The semen volume (ml/ejaculate) and sperm concentration (×106) were found to be 6.13±0.28 and 1664.28±62.41 in Holstein Friesian breeding bulls whereas 5.26±0.17 and 2036.00±43.99 in Sahiwal bulls, respectively. The progressive motility, fast motility, slow motility and local motility were significantly (p<0.05) higher in Holstein Friesian bulls than that of Sahiwal bulls both before and after freezing. Different motility patterns such as progressive, fast, slow, local and immotile motility were found to be 89.22±0.45%, 75.97±0.67%, 9.10±0.36%, 4.25±0.15% and 10.05±0.41% in Holstein Friesian bulls whereas 84.98±0.89%, 65.99±0.92%, 11.88±0.37%, 5.65±0.19% and 15.69±0.54% in Sahiwal breeding bulls, respectively before freezing. On the other hand, after freezing, the progressive, fast, slow, local and immotile spermatozoa were found to be 68.19±0.46%, 53.39±2.01%, 9.21±0.46%, 8.47±0.72% and 26.76±2.01% in Holstein Friesian bulls whereas 56.54±0.25%, 42.52±0.67%, 11.11±0.55%, 13.04±1.24% and 30.45±1.58% in Sahiwal bulls, respectively. Non-return rate was found insignificant between the breeds and it was 64.19% in Holstein Friesian and 64.71% in Sahiwal bulls. In conclusion, frozen semen quality of Holstein Friesian pure breed is better than that of Sahiwal breeding bulls but fertility of both breeds are similar. Therefore, frozen semen of both breeds can be used in the field level for the genetic improvement of cattle. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 33-41

149 The second isoform of gonadotrophin-releasing hormone and its receptor affect boar semen quality

2020 ◽  
Vol 32 (2) ◽  
pp. 201
Author(s):  
C. E. Ross ◽  
F. H. Choat ◽  
K. N. Plager ◽  
A. T. Desaulniers ◽  
R. A. Cederberg ◽  
...  
Keyword(s):  
Semen Quality ◽  
Computer Assisted ◽  
Functional Protein ◽  
Littermate Control ◽  
Normal Morphology ◽  
Releasing Hormone ◽  
Sperm Analysis ◽  
Sperm Characteristics ◽  
Straight Line ◽  
Gonadotrophin Releasing Hormone

Pigs are the only livestock species encoding a functional protein for both the second isoform of gonadotrophin-releasing hormone (GnRH-II) and its cognate receptor (GnRHR-II). Unlike the classical GnRH system (GnRH-I and GnRHR-I), GnRH-II and GnRHR-II are abundantly produced in porcine testes. Moreover, GnRH-II binding its receptor on Leydig cells stimulates luteinizing hormone-independent testosterone secretion. Interestingly, GnRHR-II is also localised to the connecting piece of mature, ejaculated spermatozoa, whereas GnRH-II is detected in seminal plasma, an interaction possibly influencing the function of sperm. To examine the role of GnRH-II and its receptor in the testis, we produced a swine line with reduced endogenous GnRHR-II levels (GnRHR-II KD). The objectives of this study were to (1) compare sperm characteristics between mature GnRHR-II KD and littermate control boars on the day of collection and following semen extension and (2) determine whether a GnRHR-I and GnRHR-II antagonist alters sperm characteristics after storage of extended semen. In Experiment 1, GnRHR-II KD (n=3) and littermate control (n=3) ejaculates were collected (Day 1) and computer-assisted sperm analysis (CASA) was performed (IVOS II Animal; Hamilton Thorne) to determine measures of sperm motion (motility, progressive motility, slow, and static), morphology (normal morphology, bent tail, coiled tail, distal droplet, proximal droplet (PD), distal midpiece reflex, elongation, and area), and kinematics (length of average path (DAP), length of straight line path (DSL), length of curvilinear path (DCL), average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), beat-cross frequency, and wobble (WOB)). Next, 3 billion sperm were extended with Androstar Plus (80-mL doses; Minitube) and stored at 17°C until Day 7 CASA. Data were analysed with the MIXED procedure of SAS (SAS Institute Inc.). On Day 1, semen doses from GnRHR-II KD boars had reduced DSL, VSL, STR, LIN, and WOB (P&lt;0.05), whereas sperm from control boars possessed more PD (P&lt;0.01). Day 7 CASA revealed that transgenic sperm had reduced DAP, DCL, VAP, and VCL, although sperm from control boars were slower (P&lt;0.05). In Experiment 2, control ejaculates (n=3) were extended as above, treated with increasing concentrations (0, 0.0001, 0.001, 0.01, 0.1, 1, and 10μM) of a GnRH antagonist inhibiting both GnRHR-I and GnRHR-II (SB-75, cetrorelix), and stored at 17°C until Day 7 and 9 CASA. On Day 7, only sperm characteristics in doses treated with 10μM SB-75 were significantly lower (normal morphology, DAP, DCL, VAP, VCL, and ALH) or higher (PD, WOB, and area) than controls. Similar differences (except ALH; P&lt;0.10) for the 10μM SB-75 treatment were detected on Day 9; however, motility, slow, static, STR, and LIN were also reduced (P&lt;0.05). Thus, these data suggest that GnRH-II and its receptor are important to sperm function, representing a potential avenue to improve semen preservation. This research was funded by USDA/NIFA AFRI (2017-67015-26508; BRW).

scholarly journals Influence of age on the spermiogramic parameters of native sheep

1970 ◽  
Vol 7 (2) ◽  
pp. 301-304 ◽  
Author(s):  
MR Hassan ◽  
S Pervage ◽  
M Ershaduzzaman ◽  
MAI Talukder
Keyword(s):  
Semen Quality ◽  
Age Groups ◽  
Sperm Concentration ◽  
Live Weight ◽  
Scrotal Circumference ◽  
Semen Volume ◽  
Native Sheep

The study was conducted to evaluate the qualities of ram semen in different age groups (one to four years) were evaluated. The parameters included testes length (cm), testes breadth (cm), scrotal circumference (cm), live weight (kg), semen volume (ml), PH, sperm concentration (x109), motility (%), percentage of live, dead and normal, abnormal spermatozoa of native sheep. The average semen volume (ml) found to be 0.60, 0.91, 0.85 and 0.87; sperm concentration (109/ml) 1.03, 3.27, 4.45 and 4.17 and finally motility (%) were found to be 68.07, 75.03, 75.01 and 76.25 respectively for the 1st, 2nd, 3rd and 4th year age of native ram. The effects of age on all parameters (testes length, semen volume, semen pH, sperm concentration, motility, viability, normal spermatozoa) were significant except testes breadth and scrotal circumference. The value of all of these parameters increased linearly up to three years with the increase of age except PH. The result of the present study indicated that scrotal diameter, serving capacity; normal and live spermatozoa were comparatively better during the 3rd year than the 1st and 2nd year of age while the values were almost similar at 3rd and 4th year of age. With the increasing of age, the semen quality improved and stabilized up to at the age of 3 years. Keywords: Native sheep; Spermiogramic parameter; Age DOI: 10.3329/jbau.v7i2.4738 J. Bangladesh Agril. Univ. 7(2): 301-304, 2009

scholarly journals Seasonal variations in quantitative and qualitative sperm characteristics in fertile and subfertile stallions

2020 ◽  
Vol 63 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Yara Suliman ◽  
Frank Becker ◽  
Armin Tuchscherer ◽  
Klaus Wimmers
Keyword(s):  
Breeding Season ◽  
Seasonal Variations ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Computer Assisted ◽  
Normal Sperm ◽  
Straight Line ◽  
Significant Seasonal Variation

Abstract. Horses are seasonal breeders with a natural breeding season beginning in spring and extending through midsummer. In this study, quantitative and qualitative parameters of chilled stallion semen were compared between fertile and subfertile stallions and between the breeding and the non-breeding season. Semen quality parameters compared included ejaculate volume, sperm concentration, total sperm number, sperm morphology, and computer-assisted semen analysis (CASA)-derived sperm movement characteristics obtained from two groups of warmblood stallions (n=8; four fertile stallions and four subfertile stallions), which differ in the seasonal pregnancy rate 80 %–90 % (fertile) vs. 40 %–60 % (subfertile). A total of 64 ejaculates were collected from the stallions (n=8; four in the breeding season and four in the non-breeding season of each stallion). No significant differences in the semen quality parameters between the fertile and the subfertile stallions in the non-breeding season were observed. However, in the breeding season the proportion of morphologically normal sperm, total motility, progressive motility, average path velocity (VAP), and curvilinear velocity (VCL) were significantly higher in the fertile group (P<0.05) when compared with the subfertile group. In addition, a significant seasonal variation in the proportion of morphological normal sperm was found in the fertile group between the breeding and the non-breeding season (P<0.05). Moreover, significant seasonal variations (P<0.05) in CASA parameters of mean VAP, straight line velocity (VSL), and beat-cross frequency (BCF) were observed in the fertile and the subfertile stallions, which tended to be lower in the non-breeding season. In conclusion, differences between the fertile and the subfertile stallions were observed only in the breeding season, and a few of CASA-derived parameters seemed to be significantly lower during the non-breeding season in both the fertile and the subfertile stallions.

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Kaji Banding Kualitas Semen Segar Empat Genetik Ayam Lokal Indonesia (COMPARATIVE STUDY ON THE QUALITY OF FRESH SEMEN OF FOUR GENETIC LOCAL CHICKEN IN INDONESIA)

2019 ◽  
Vol 20 (3) ◽  
pp. 397
Author(s):  
Junaedi Junaedi ◽  
Husnaeni Husnaeni
Keyword(s):  
Comparative Study ◽  
Spermatozoa Motility ◽  
Genetic Groups ◽  
Randomized Design ◽  
Semen Volume ◽  
Completely Randomized Design ◽  
Local Chicken ◽  
Fresh Semen ◽  
Better Than

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.

SCROTAL CIRCUMFERENCE, SEMEN CHARACTERISTICS, GROWTH PARAMETERS AND THEIR RELATIONSHIPS IN YOUNG BEEF BULLS

1985 ◽  
Vol 65 (4) ◽  
pp. 789-798 ◽  
Author(s):  
M. MAKARECHIAN ◽  
A. FARID ◽  
R. T. BERG
Keyword(s):  
Semen Quality ◽  
Growth Parameters ◽  
Scrotal Circumference ◽  
Three Samples ◽  
Semen Volume ◽  
Semen Characteristics ◽  
The Mean ◽  
Beef Bulls ◽  
The Relationship ◽  
Daily Gain

The records of sixty-six yearling and 2-yr-old beef bulls selected for breeding in 1980 and 1981 were used to study the relationships between body weight (BW) and gain measurements, scrotal circumference (SC) and semen characteristics in young beef bulls. The bulls belonged to four breed groups; Hereford, Beef Synthetic, Dairy Synthetic and Pee Wee. A week before the start of breeding season, the bulls were weighed and measured for scrotal circumference. A single prebreeding semen sample was collected by electro-ejaculator from each bull in 1980, while three samples (pre-, mid- and postbreeding) were collected by artificial vagina in 1981. The mean SC ranged from 31.3 cm in the Pee Wee to 38.6 cm in the Beef Synthetic, while SC per 100 kg BW was maximum in the Pee Wee (8.36%) and minimum in the Dairy Synthetic group (6.28%, P < 0.05). The relationship between BW and SC was linear within yearling and 2-yr-old bulls. Age did not have any effect on SC over and above BW within each age group. Preweaning daily gain accounted for a higher fraction of variation in SC compared to BW in all the breed groups except Dairy Synthetic. The relationships between BW, SC and feedlot daily gain with semen quality and quantity were positive but generally small (r < 0.30, P > 0.11). Semen volume and sperm motility had lower values and percent secondary abnormalities were higher in prebreeding compared with mid- or postbreeding samples. Repeatability estimates of the semen characteristics were generally low, ranging from 0 to 0.49. Key words: Beef bulls, scrotal circumference, semen characteristics, growth rate

scholarly journals Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1506
Author(s):  
Hongtao Wang ◽  
Ping Lu ◽  
Chongshan Yuan ◽  
Jing Zhao ◽  
Hongyu Liu ◽  
...  
Keyword(s):  
Semen Analysis ◽  
Computer Assisted ◽  
Astragalus Polysaccharides ◽  
Bovine Semen ◽  
Bull Semen ◽  
Straight Line ◽  
Frozen Semen ◽  
Path Distance ◽  
Straight Line Distance

The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis (CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (p < 0.05), while reducing the ROS and MDA levels (p < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.

9 Single Layer Centrifugation of Bull Semen Through Percoll Plus® Before Cryopreservation

2018 ◽  
Vol 30 (1) ◽  
pp. 144
Author(s):  
A. Martins ◽  
F. N. Marqui ◽  
T. E. Cruz ◽  
T. I. H. Berton ◽  
D. G. Souza ◽  
...  
Keyword(s):  
Semen Quality ◽  
Single Layer ◽  
Computer Assisted ◽  
Treatment Groups ◽  
Progressive Motility ◽  
Bull Semen ◽  
Head Displacement ◽  
Straight Line ◽  
Artificial Vagina ◽  
Lateral Head

We previously reported that single layer centrifugation (SLC) with Percoll® (GE Healthcare, Uppsala, Sweden) of fresh bovine semen resulted in improved sperm progressive motility and movement, as evidenced by computer-assisted sperm analyzer (CASA) after freezing-thawing. However, no report has been found in the literature on the use of Percoll Plus® (PP; GE Healthcare), a nontoxic colloid, for the same purpose. Thus, this study aimed to verify the effects of SLC-PP before bull sperm freezing on sperm kinematics after cryopreservation. Ejaculates were collected from 3 Nellore bulls (6 from each) using an artificial vagina. After collection, the semen was assessed and pooled, and then 1 billion spermatozoa either diluted [D; 1:2 (v/v)] in freezing extender (FE, without glycerol) or undiluted (UD) was layered on top of a 9-mL column of PP (in 15-mL centrifuge tubes) at concentrations of 70% or 90% to form the 70D, 70UD, 90D, and 90UD treatment groups. Following centrifugation for 13 min at 839 × g [except for the control (C) group], the supernatant was removed and the sperm pellet diluted to 50 × 106 sperm mL−1 in FE medium plus glycerol. Then, frozen–thawed sperm samples were analysed by CASA (MMC Sperm, St. Petersburg, Russia) for the following parameters: total motility (TM, %), progressive motility (PM, %), curvilinear velocity (VCL, µm−1), straight line velocity (VSL, µm s−1), average path velocity (VAP, µm s−1), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz), linearity (LIN, %), and straightness (STR, %). For statistical analyses, ANOVA and Student-Newman-Keuls test were used. Data are presented as mean ± SEM with P < 0.05 taken as significant. No difference was found among the groups for TM, VSL, BCF, and STR. However, the percentage of PM was higher (P < 0.05) in the SLC-selected sperm samples (values ranging from 42.0 ± 7.0 to 47.4 ± 11.4) than in C (28.8 ± 5.0), and ALH was lower in 70UD (1.6 ± 0.12) and 70D (1.7 ± 0.10) than in C (1.9 ± 0.2). Moreover, 70UD (49.0 ± 1.0), 90UD (50.0 ± 3.0), and 90D (50.0 ± 4.0) displayed higher percentage of LIN (P < 0.05) compared with C (45.0 ± 2.0) and 70D (48.0 ± 3.0). On the other hand, similar results were obtained for VCL (from 126.3 ± 8.0 to 130.0 ± 20.5) and VAP (from 82.7 ± 14.5 to 85.1 ± 6.9) in C, 70UD, and 70D, but these values differed (P < 0.05) from those for VCL in 90UD (104.6 ± 10.3) and 90D (97.2 ± 22.0) as well as for VAP in 90UD (72.2 ± 11.0) and 90D (71.8 ± 9.6). These are the first data demonstrating favourable influences of SLC with 70% Percoll Plus® to select distinct sperm subpopulations as evidenced by enhanced PM, LIN, and ALH. Thus, SLC-PP could optimize the production of frozen bull semen by decreasing the number of sperm per insemination dose, and help to circumvent limitations associated with the poor semen quality sometimes found in bulls of high genetic merit. This research was funded by FAPESP # 2015/20986-3, MasterFertility and Tairana Artificial Insemination Station, Brazil.

Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls 

2018 ◽  
Author(s):  
K.B. Iyer ◽  
M.V. Ingawale ◽  
S.V. Kuralkar ◽  
P.S. Bankar ◽  
S. Sajid Ali ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Semen Quality ◽  
Receptor Gene ◽  
Gnrh Receptor ◽  
Integrity Test ◽  
Osmotic Swelling ◽  
Scrotal Circumference ◽  
Receptor Gene Polymorphism ◽  
Abnormal Sperm ◽  
Semen Volume

The present research was conducted to study the association of GnRH receptor gene polymorphism on testicular parameters and semen quality in fortyfour HF crossbred bulls. Two different genotypes GG and GA having amplification size 170, 118, 96 and 214, 170, 118 were identified, respectively. Genotypic frequencies were 0.82 (GG) and 0.18(GA) and gene frequency of G allele was 0.91 and for A allele was 0.09. The testicular parameters like scrotal circumference (SC), testicular width (TW), testicular thickness (TT) were non significant while testicular length (TL) was significant (P less than 0.05) among two genotypes. Seminal attributes like semen volume (Vol) concentration (Conc.) initial motility (IM), post thaw motility (PTM), abnormal sperm rate (ASR) and acrosome integrity test (AIT) were non significant while hypo osmotic swelling test (HOST) was significant (P less than 0.05) among two genotypes. 

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