Mitigation of sterigmatocystin exposure in cattle by difructose anhydride III feed supplementation and detection of urinary sterigmatocystin and serum  amyloid A concentrations

Abstract. We evaluated the effects of supplementing cattle feed with difructose anhydride III (DFA III) by measuring urinary sterigmatocystin (STC) concentrations using 20 Japanese Black cattle aged 9–10 months from one herd. DFA III was supplemented for 2 weeks for 10 animals, and non-treated animals served as controls. The natural STC concentration in the dietary feed was 0.06 mg kg−1 (mixture of roughage and concentrate) at the beginning of the study (Day 0). The urine STC concentration was measured using liquid chromatography with tandem mass spectrometry 1 d prior to DFA III administration, 9 and 14 d thereafter, and 9 d following supplementation cessation, concomitant with the measurement of serum amyloid A (SAA). The number of heifers in which STC was detected in the urine was low (10 %) in the DFA III group compared to that (60 %) in the control group on Day 9. After 9 d following supplementation cessation (Day 23), STC concentrations were significantly lower (P=0.032) in the DFA III group than in the control group, although there was no difference in the number of heifers in which urinary STC was detected or in SAA concentrations between the two groups. Our findings demonstrate the effect of DFA III on reducing the urinary concentration of STC in Japanese Black cattle.

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Physiotherapy protocol during initial postoperative period of arthroscopy in horses

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5655 ◽

2018 ◽

Vol 38 (12) ◽

pp. 2201-2206

Author(s):

Fernanda C. Stievani ◽

Thais S.L. Machado ◽

Kaio B. Bezerra ◽

Marilene M. Silva ◽

Raquel Y.A. Baccarin ◽

...

Keyword(s):

Synovial Fluid ◽

Physical Examination ◽

Postoperative Period ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Control Group ◽

Physical Analysis ◽

Amyloid A ◽

Significant Difference ◽

Significant Change

ABSTRACT: This study evaluated the effects of a physiotherapy protocol applied in joints with osteochondritis dissecans submitted to arthroscopy. Twelve horses totaling twenty joints were used and divided into two uniform groups, according to articular lesion grade. Treated Group (TG) received the physiotherapy protocol (cryotherapy, passive rage motion and controlled exercise) that initiate just after anesthetic recovery and extended for five days. Control Group (CG) remained resting in stall during the same period. Physical examination and synovial fluid analysis were used to evaluate the treatment. The synovial fluid examination consisted of physical analysis (color, aspect, and viscosity), mucin clot evaluation, Serum Amyloid A, Prostaglandin E2 and urea concentration. Synovial samples were collected by arthrocentesis at the beginning of the surgical procedure (D1), 48 hours (D3) and 96 hours (D5) after surgery. Before arthroscopy and daily during the postoperative period joints were evaluated by physical exam: superficial temperature (°C), range of motion (degrees) and circumference (centimeters). The joint physical examination showed no significant difference between groups and neither along the days for the same group. The parameters of synovial fluid showed difference over the moments in each group but didn’t have difference between groups. Color and aspect had the same patterns across moments, in CG fluid had significant change when compared D1 with D3 (color and aspect: p<0.001) and D5 (color: p<0.001; aspect: p<0.05) becoming mostly bloody and cloudy in D3 and D5. However in TG the difference was significant just between D1 and D3 (color and aspect: p<0.05), showing an improvement of synovial fluid in D5 (color and aspect: p>0.05). Viscosity and mucin clot evaluation showed significant change in CG between D1 and D3 (viscosity: p<0.01; mucin clot: p<0.05) and between D1 and D5 (viscosity: p<0.01;mucin clot: p<0.01). In TG no significant difference of viscosity and mucin clot was observed over the moments, showing an early improvement of synovial fluid quality. The Serum Amyloid A concentration showed an extremely significant increase in CG (p<0.001) when compared D1 (1217.13±664.47μg/mL) and D3 (42423.80±52309.31μg/mL). The comparison between D1 and D5 in CG, and across moments in TG, had no statistical difference. The PGE2 eicosanoid remained statistically unchanged all over the time. Urea showed significant increase in D3 when compared to D1 (p<0.001) in CG, and had no variation in TG. The physiotherapy protocol minimized the inflammatory mediators and provided minor alterations in synovial fluid after arthroscopy.

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Detection of serum amyloid-A concentration in the calf clinically diagnosed with pneumonia, enteritis and pneumoenteritis

Ciência Rural ◽

10.1590/0103-8478cr20150571 ◽

2016 ◽

Vol 46 (2) ◽

pp. 293-299 ◽

Cited By ~ 1

Author(s):

Mustafa Kabu ◽

Bulent Elitok ◽

Ismail Kucukkurt

Keyword(s):

Veterinary Medicine ◽

Blood Cell ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Control Group ◽

Amyloid A ◽

Tnf Α ◽

Concentration Measurements ◽

Severity Of The Disease ◽

Commercial Kits

ABSTRACT: The aim of this study is to determine serum amyloid-A (SAA) concentration in the cases of pneumonia, pneumoenteritis, and enteritis which are frequently encountered in calves in veterinary medicine. Although a great deal of experimental studies has been conducted in this field, studies on naturally infected calves are quite few. Eighty calves at the age of 0-6 months were used in the study and the calves were divided into four groups. Due to the clinical examination, the calves diagnosed with pneumonia (Group P; n=20), with pneumoenteritis (Group PE; n=20) and with enteritis (Group E; n=20) formed the disease group as the healthy ones formed the control (Group C; n=20) group. After the body temperatures of all calves were taken, blood samples were obtained from Jugular vein for haematological and biochemical measurements. As haematological, white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) measurements were performed in Veterinary Hematology Analyzer. Serum amyloid-A (SAA), interleukin 1 (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) concentration measurements were carried out with ELISA reader by using commercial kits. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin (ALB), total bilirubin (T. Bil), total protein (TP), gamma glutamyltransferase (GGT), blood urea nitrogen (BUN) concentration measurements were conducted in autoanalyzer by using commercial kits. In all disease groups (P, PE, and E) body temperature, haematologic parameters (WBC, RBC, Hb and Hct), serum biochemical parameters (AST, ALT, ALB, T. Bil, TP, GGT and BUN), SAA concentration and serum concentrations of cytokines (IL-1β, IL-6 and TNF-α) were determined to be higher in comparison to the control group (P<0.005). According to these findings, routine measurement of serum SAA concentration in veterinary medicine is considered to be beneficial in determining the severity of the disease, in selecting the proper treatment, in monitoring the applied treatment, and detecting subclinical diseases. In the light of these findings we acknowledge that routine measurements of serum SAA concentration from the moment the calves are diagnosed with pneumonia, enteritis and pneumoenteritis in veterinary medicine until the actual cause is determined (bacteria, virus, parasites, etc.) would avail the clinician to, identify the severity of the disease, select the appropriate treatment and monitor the effectiveness of the treatment.

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Serum Amyloid A Level in Women with Unexplained Infertility

QJM ◽

10.1093/qjmed/hcab115.039 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Mostafa Ibrahim ◽

Hayam Fathy Mohammad ◽

Nashwa Nagy el-Khazragy ◽

Zeinab Saad Abd El wahab

Keyword(s):

Research Group ◽

Inflammatory Process ◽

Serum Amyloid A ◽

Research Study ◽

Maternity Hospital ◽

Unexplained Infertility ◽

Serum Amyloid ◽

Control Group ◽

Chronic Inflammatory Process ◽

Amyloid A

Abstract Background By various molecular and cellular research efforts it was displayed that folliculogenesis, ovulation, and corpus luteum formation is considered as an inflammatory processes that hav raised the research interest to investigate that serum amyloid A as an inflammatory mediator to reveal its possible role in infertility. Objective This study aims to assess the association between SAA and unexplained infertility in women. Methods The current research study have been conducted at Ain Shams University Maternity Hospital during the period from September 2018 to April 2019. Women approached were recruited from outpatient clinic of Ain Shams University Maternity Hospital. The recruited 90 research study subjects have been divided into two research groups Results Serum amyloid A was statistically significantly higher among the unexplained research group versus control group (SAA had excellent discriminative value with an area under the ROC curve (AUC) of 0.992 (95% CI = 0.945 to 1.000, Pvalue <0.0001). A best cutoff criterion is SAA of 28.7 mg/l (sensitivity = 98%, specificity = 96%, J-index = 0.93)) denoting that amyloid A as an acute phase inflammatory modulator is higher among the unexplained research group reflecting a form of chronic inflammatory process in those category of cases. Analytical research results of multivariable binary logistic regression analysis as regards the correlation between SAA and unexplained infertility after adjustment for age and BMI, it was revealed that serum amyloid A an independent predictor for unexplained infertility. Conclusion s: Serum amyloid A as a biomarker for could be implemented as a predictability tool for unexplained infertility since it is a reflector of an chronic inflammatory process that is ongoing that could affect the process of implantation and ovulation however the clear molecular and cellular processes linked to the pathophysiological process that reflects the fertility hindering process should be implemented in future research efforts taking in consideration to have multicentric fashion of research with larger sample sizes to verify the value of serum amyloid A as biomarker that reflects the fertility potential besides the routine infertility work up.

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The serum protein electrophoretic pattern and acute phase protein concentrations in calves with chronic respiratory diseases

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0052 ◽

2013 ◽

Vol 16 (2) ◽

pp. 385-386 ◽

Cited By ~ 2

Author(s):

Cs. Tóthová ◽

O. Nagy ◽

G. Kováč

Keyword(s):

Serum Protein ◽

Acute Phase ◽

Respiratory Diseases ◽

Serum Amyloid A ◽

Electrophoretic Pattern ◽

Serum Amyloid ◽

Control Group ◽

Chronic Respiratory Diseases ◽

Amyloid A ◽

Protein Electrophoretic Pattern

Abstract The aim of this study was to evaluate the serum protein electrophoretic pattern and the concentrations of acute phase proteins (haptoglobin, serum amyloid A, and fibrinogen) in 28 calves with clinical signs of chronic respiratory diseases and 36 healthy calves as a control group. In sick calves we found significantly higher serum concentrations of total proteins (P<0.001), lower concentrations of albumin (P<0.001) and marked shift in the concentrations of the most of protein fractions with significantly higher values of α1-, β1-, β2-, and γ-globulins (P<0.001 and P<0.01). The affected calves had significantly higher values of haptoglobin, serum amyloid A, and fibrinogen as well (P<0.05, P<0.001 and P<0.001, respectively).

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Circulating Serum Amyloid A, hs-CRP and Vitamin D Levels in Postmenopausal Osteoporosis

10.31661/gmj.v8i0.1548 ◽

2019 ◽

Vol 8 ◽

Author(s):

Anahid Safari ◽

Afshin Borhani-Haghighi ◽

Mehdi Dianatpour ◽

Seyed Taghi Heydari ◽

Farzaneh Foroughinia ◽

...

Keyword(s):

Vitamin D ◽

Postmenopausal Osteoporosis ◽

Serum Amyloid A ◽

Serum Levels ◽

Serum Amyloid ◽

Control Group ◽

Mineral Density ◽

Amyloid A ◽

Vitamin D Levels ◽

Hs Crp

Background: Both vitamin D and inflammation were investigated as important players in the pathogenesis of postmenopausal osteoporosis. This study compared vitamin D, inflammatory the biomarkers serum levels and their association with bone mineral density (BMD) in case and control groups to evaluate the possible immune-regulatory effect of vitamin D in this population. Materials and Methods: Participants in post-menopausal age, were categorized to 44 osteoporotic vs. 44 healthy aged-matched women according to WHO criteria. Total BMD, T- scores, Z-scores as well as fracture risk were measured in both groups, using Hologic system Dual-energy X-ray absorptiometry (DEXA). Serum 25-OH vitamin D, high sensitive CRP (hs-CRP) and serum amyloid A (SAA) were compared between groups. The association between serum biomarkers level and BMD were also investigated. The same evaluations were performed for vitamin D deficient (<20 ng/mL) and non-deficient (≥20 ng/mL) subgroups. Results: Vitamin D deficiency was higher in the osteoporotic group (32.6%) in comparison with the control group (25.6%), but the differences were not significant (P=0.47). There were no significant differences in serum levels of hs-CRP and SAA (P=0.83 and P=0.39) as well. No significant association between serum inflammatory biomarkers, vitamin D, and BMD were detected (P≥0.05). The results were the same for vitamin D deficient and non-deficient subgroups (P≥0.05). Conclusion: In the current study, the beneficial effects of vitamin D as a result of its immune-regulatory mechanisms was not reached. Larger scale studies might pave the way to define vitamin D benefits in postmenopausal osteoporosis. [GMJ.2019;8:e1548]

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Serum amyloid A protein levels as a possible aid in the diagnosis of acute appendicitis in children

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2005.007 ◽

2005 ◽

Vol 43 (1) ◽

Cited By ~ 15

Author(s):

Lilia Lycopoulou ◽

Charalambos Mamoulakis ◽

Eugenia Hantzi ◽

Dimitrios Demetriadis ◽

Spyros Antypas ◽

...

Keyword(s):

Acute Appendicitis ◽

Sensitivity And Specificity ◽

Serum Amyloid A ◽

Area Under The Curve ◽

Serum Amyloid ◽

Control Group ◽

Biochemical Tests ◽

Acute Phase Reactants ◽

Amyloid A ◽

Serum Amyloid A Protein

AbstractHematological and biochemical tests, including white blood cell count (WBC), C-reactive protein (CRP) and other acute-phase reactants, have been used in the diagnosis of acute appendicitis. However, there is controversy among physicians about the value of this practice in children. The objective of our study was to evaluate serum amyloid A protein (SAA) levels in children with confirmed acute appendicitis and to compare the sensitivity and specificity of this marker of inflammation with those for WBC and CRP. A prospective cohort study of 60 children admitted with abdominal pain to rule out appendicitis was used in the study. Of these, 42 underwent surgery, while 18 children who had spontaneous amelioration within 24h of admission were not operated on and served as controls. WBC and serum SAA and CRP levels were obtained preoperatively. Serum concentrations of the analytes were determined with particle-enhanced immunonephelometric methods. Patients with acute appendicitis had WBC, SAA and CRP levels higher than those of the control group (p<0.001). There was no appendicitis patient with a normal SAA value, while 21.4% of the patients had CRP values within the normal range. The performance of each test was measured by receiver-operating characteristic curves. Area under the curve (AUC) values were 0.849 for WBC, 0.868 for CRP and 0.964 for SAA. The sensitivity and specificity of these methods were 76% and 75% for WBC>10.0×10

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