scholarly journals Nitrogen fixation and the diazotroph community in the temperate coastal region of the northwestern North Pacific

2015 ◽  
Vol 12 (15) ◽  
pp. 4751-4764 ◽  
Author(s):  
T. Shiozaki ◽  
T. Nagata ◽  
M. Ijichi ◽  
K. Furuya
Keyword(s):  
Nitrogen Fixation ◽  
North Pacific ◽  
Nifh Gene ◽  
Anaerobic Bacteria ◽  
Coastal Region ◽  
Late Summer ◽  
Euphotic Zone ◽  
Early Summer ◽  
Pcr Analysis ◽  
Subsurface Layers

Abstract. Nitrogen fixation in temperate oceans is a potentially important, but poorly understood process that may influence the marine nitrogen budget. This study determined seasonal variations in nitrogen fixation and the diazotroph community within the euphotic zone in the temperate coastal region of the northwestern North Pacific. Nitrogen fixation as high as 13.6 nmol N L−1 d−1 was measured from early summer to fall when the surface temperature exceeded 14.2 °C (but was lower than 24.3 °C) and the surface nitrate concentration was low (≤ 0.30 μM), although we also detected nitrogen fixation in subsurface layers (42–62 m) where nitrate concentrations were high (> 1 μM). Clone library analysis results indicated that nifH gene sequences were omnipresent throughout the investigation period. During the period when nitrogen fixation was detected (early summer to fall), the genes affiliated with UCYN-A, Trichodesmium, and γ-proteobacterial phylotype γ-24774A11 were frequently recovered. In contrast, when nitrogen fixation was undetectable (winter to spring), many sequences affiliated with Cluster III diazotrophs (putative anaerobic bacteria) were recovered. Quantitative PCR analysis revealed that UCYN-A was relatively abundant from early to late summer compared with Trichodesmium and γ-24774A11, whereas Trichodesmium abundance was the highest among the three groups during fall.

scholarly journals Seasonal dynamics of nitrogen fixation and the diazotroph community in the temperate coastal region of the northwestern North Pacific

2015 ◽  
Vol 12 (1) ◽  
pp. 865-889
Author(s):  
T. Shiozaki ◽  
T. Nagata ◽  
M. Ijichi ◽  
K. Furuya
Keyword(s):  
Nitrogen Fixation ◽  
North Pacific ◽  
Gene Diversity ◽  
Nifh Gene ◽  
Anaerobic Bacteria ◽  
Coastal Region ◽  
Euphotic Zone ◽  
Early Summer ◽  
Subsurface Layers ◽  
Nifh Genes

Abstract. Nitrogen fixation in temperate oceans is a potentially important, but poorly understood process that may influence the marine nitrogen budget. This study determined seasonal variations in nitrogen fixation and nifH gene diversity within the euphotic zone in the temperate coastal region of the northwestern North Pacific. Nitrogen fixation as high as 13.6 nmolN L−1 d−1 was measured from early summer to fall when the surface temperature exceeded 14.2 °C and the surface nitrate concentration was low (≤ 0.30 μM), although we also detected nitrogen fixation in subsurface layers (42–62 m) where nitrate concentrations were high (> 1 μM). During periods with high nitrogen fixation, the nifH sequences of UCYN-A were recovered, suggesting that these groups played a key role in nitrogen fixation. The nifH genes were also recovered in spring and winter when nitrogen fixation was undetectable. These genes consisted of many sequences affiliated with Cluster III diazotrophs (putative anaerobic bacteria), which hitherto have rarely been reported to be abundant in surface diazotroph communities in marine environments.

Effects of Dried Distillers’ Grains Cube Supplementation for Steers Grazing Introduced Pastures on Animal Performance and Forage Production

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 12-13
Author(s):  
Jordan Adams ◽  
Rodney Farris ◽  
Scott Clawson ◽  
Earl Ward ◽  
Paul Beck
Keyword(s):  
Festuca Arundinacea ◽  
Statistical Power ◽  
Cynodon Dactylon ◽  
Animal Health ◽  
Late Summer ◽  
Early Summer ◽  
Distillers Grains ◽  
Forage Production ◽  
Trenbolone Acetate ◽  
Dried Distillers Grains

Abstract We evaluated the effects of supplementing dried distillers’ grains cubes (DDGS) and re-implantation of steers (n = 149; BW = 238 ± 13.8 kg) grazing tall fescue (Festuca arundinacea)/bermudagrass (Cynodon dactylon) pastures (n = 9 pastures, 7.2 ± 2.90 ha) from 14 April to 17 September 2020 (n = 155 d) in a split-plot design on steer performance and forage production. Main plot supplemental treatments (n = 3 pastures/treatment) included 1) Fertilized Control (FC), no supplementation on fertilized pastures (112 kg N/ha); 2) Fertilized Supplement (FS), supplemental DDGS fed at 2.9 kg 3-d/wk on fertilized pastures; and 3) Supplement (S), supplemented DDGS at 0.75% BW/d on unfertilized pastures prorated for 5-d/wk feeding. Steers were previously implanted during receiving with 40 mg trenbolone acetate and 8 mg estradiol (REV-G; Revalor G, Merck Animal Health). On July 7, steers in each pasture were randomly assigned to one of three re-implant treatments: 1) no re-implant; 2) REV-G; or 3) 200 mg progesterone and 20 mg estradiol (Synovex S, Zoetis Animal Health). Steers in FS and S gained more (P < 0.01) than FC throughout the trial and final BW was greater (P < 0.01) for FS and S compared with FC. Unexpectedly, re-implanting had no effect on ADG (P = 0.57) or BW (P = 0.34), but statistical power may be lacking. Supplemental efficiency was greater in the late summer for FS (P = 0.05) compared to S. Fertilizing pastures in FS and FC did not affect biomass (P = 0.39), however, CP was increased (P = 0.01) and acid and neutral detergent fibers tended to decrease (P = 0.06) relative to S in the early summer (April, May, June, and July), but did not differ in late summer (August and September). Based upon our analysis, DDGS is a suitable supplement and can replace N fertilizer for steers grazing introduced pastures.

scholarly journals 179 Using a 100% Dried Distillers Grains Cube as a Supplement for Steers Grazing Mixed Grass Prairie in Oklahoma

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 46-46
Author(s):  
Zane N Grigsby ◽  
Paul A Beck ◽  
Stacey A Gunter
Keyword(s):  
Animal Health ◽  
Late Summer ◽  
Estradiol Benzoate ◽  
Positive Control ◽  
Negative Control ◽  
Early Summer ◽  
Trenbolone Acetate ◽  
Mixed Grass Prairie ◽  
Mixed Grass ◽  
Main Plot

Abstract This research was conducted to determine effects of supplementation and implanting on BW gain by steers grazing mixed grass prairie (n = 12 pastures, 19.9 ± 0.7 ha) in northwest Oklahoma. Three main plot treatments were: 1) Negative Control (NC), no supplementation, 2) Positive Control (PC), supplemented with DDGS cubes, 1.8 kg/steer on alternate days in late summer, 3) High Supplement (HS), 1/3 increase in stocking rate with 0.75% BW supplemental DDGS cubes all season. Steers (n = 125, BW = 223.1 ± 23.2 kg) were stocked at 2.2 ha/steer for PC and NC, 1.3 ha/steer for HS. Grazing was from May 17 – September 27 (132 d). All steers were implanted with 200 mg progesterone and 20 mg estradiol benzoate (SYN, Synonvex S, Zoetis Animal Health) on May 17. On July 18 three reimplant treatments were applied: 1) no reimplant; 2) SYN; or 3) 40 mg trenbolone acetate and 8 mg estradiol (Revalor G, Merck Animal Health). Data were analyzed using the PROC MIXED in SAS as a split-plot experimental design. In early summer HS had 0.26 kg greater (P < 0.01) ADG than NC and PC. Late summer gains of PC were 0.33 kg/d more (P ≤ 0.01) than NC; and HS gained 0.49 and 0.16 kg/day more (P ≤ 0.04) than NC and PC, respectively. Gain per hectare for PC (46 kg/ha) were greater (P < 0.01) than NC (35 kg/ha) and more than doubled (P < 0.01) with HS (89 kg/ha). Reimplanting had no effect on ADG (P ≥ 0.28). Late season supplementation with PC resulted in supplemental efficiency of 2.7 kg supplement/kg added gain compared with NC. Increased stocking rates with season long supplementation in HS resulted in supplemental efficiency of 3.8 kg supplement/kg added gain per hectare. Based on these data, a 100% DDGS cube is an effective supplement option to increase BW gain during the late summer or increase carrying capacity and gain during the summer grazing period in northwestern Oklahoma.

scholarly journals Mutation in the ntrR Gene, a Member of the vap Gene Family, Increases the Symbiotic Efficiency of Sinorhizobium meliloti

2001 ◽  
Vol 14 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Boglárka Oláh ◽  
Erno Kiss ◽  
Zoltán Györgypál ◽  
Judit Borzi ◽  
Gyöngyi Cinege ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pathogenic Bacteria ◽  
Sinorhizobium Meliloti ◽  
Root Nodules ◽  
Nifh Gene ◽  
Dry Weight ◽  
Nodule Occupancy ◽  
Gene Encoding ◽  
Symbiotic Efficiency ◽  
Host Interactions

In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by the symbiotic partner Sinorhizobium meliloti supports the growth of the host plant in nitrogen-depleted environment. Here, we report that a derivative of S. meliloti carrying a mutation in the chromosomal ntrR gene induced nodules with enhanced nitrogen fixation capacity, resulting in an increased dry weight and nitrogen content of alfalfa. The efficient nitrogen fixation is a result of the higher expression level of the nifH gene, encoding one of the subunits of the nitrogenase enzyme, and nifA, the transcriptional regulator of the nif operon. The ntrR gene, controlled negatively by its own product and positively by the symbiotic regulator syrM, is expressed in the same zone of nodules as the nif genes. As a result of the nitrogen-tolerant phenotype of the strain, the beneficial effect of the mutation on efficiency is not abolished in the presence of the exogenous nitrogen source. The ntrR mutant is highly competitive in nodule occupancy compared with the wild-type strain. Sequence analysis of the mutant region revealed a new cluster of genes, termed the “ntrPR operon,” which is highly homologous to a group of vap-related genes of various pathogenic bacteria that are presumably implicated in bacterium-host interactions. On the basis of its favorable properties, the strain is a good candidate for future agricultural utilization.

scholarly journals Euphotic zone metabolism in the North Pacific Subtropical Gyre based on oxygen dynamics

2020 ◽  
Author(s):  
Sara Ferron ◽  
Benedetto Barone ◽  
Matthew J Church ◽  
Angelicque E. White ◽  
David M. Karl
Keyword(s):  
North Pacific ◽  
Euphotic Zone ◽  
Subtropical Gyre ◽  
North Pacific Subtropical Gyre ◽  
The North ◽  
Oxygen Dynamics ◽  
The North Pacific

Replacing synthetic N with clovers or alfalfa in bermudagrass pastures. 1. Herbage mass and pasture carrying capacity

2017 ◽  
Vol 57 (3) ◽  
pp. 539 ◽  
Author(s):  
P. Beck ◽  
T. Hess ◽  
D. Hubbell ◽  
M. S. Gadberry ◽  
J. Jennings ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Trifolium Repens ◽  
Carrying Capacity ◽  
Nutritive Value ◽  
Cynodon Dactylon ◽  
Late Summer ◽  
Early Summer ◽  
Fertilisation Rate ◽  
Medicago Sativa L ◽  
Trifolium Repens L

The objective of this study was to evaluate the effects of including alfalfa (ALF, Medicago sativa L.) or a combination of white (Trifolium repens L.) and red (Trifolium pretense L.) clovers (CLVR) inter-seeded into bermudagrass (Cynodon dactylon L. Pers.) on herbage nutritive value compared with monocultures of bermudagrass fertilised with 0 (0N), 56 (56N), or 112 (112N) kg nitrogen (N)/ha over four grazing seasons. In autumn, at the end of the fourth year and in the spring before the fifth grazing season, alfalfa and clover plants were killed and the carryover N benefit of CLVR or ALF was compared with N fertilisation rates during the fifth year. Across years, N fertilisation rate increased herbage mass and carrying capacity linearly; whereas herbage production from CLVR and ALF swards was equivalent to 56N, were greater than 0N and less than 112N. Herbage mass in CLVR and ALF swards was greater than fertilised bermudagrass swards in the spring and did not differ from fertilised bermudagrass in the early summer. In late summer herbage accumulation of CLVR and ALF swards appeared to decrease, limiting the herbage mass in the legume pastures compared with 56N and 112N. Carrying capacity of CLVR and ALF swards was greater than fertilised bermudagrass in the spring and early summer, but did not differ from fertilised swards in the late summer. The N benefit of including legumes in bermudagrass swards can alleviate the reliance on synthetic N fertilisation with little overall effect on pasture carrying capacity.

scholarly journals Biological nitrogen fixation in the oxygen-minimum region of the eastern tropical North Pacific ocean

2017 ◽  
Vol 11 (10) ◽  
pp. 2356-2367 ◽  
Author(s):  
Amal Jayakumar ◽  
Bonnie X Chang ◽  
Brittany Widner ◽  
Peter Bernhardt ◽  
Margaret R Mulholland ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pacific Ocean ◽  
North Pacific ◽  
Biological Nitrogen Fixation ◽  
North Pacific Ocean ◽  
Biological Nitrogen ◽  
Oxygen Minimum

scholarly journals Effects of Environmental Temperature onCapnodis tenebrionisAdult Phenology

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Carmelo Peter Bonsignore
Keyword(s):  
Late Summer ◽  
Early Summer ◽  
Adult Density ◽  
Degree Days ◽  
Continuous Increase ◽  
Climate Conditions ◽  
Average Annual Temperature ◽  
Activity Temperature ◽  
The Difference ◽  
New Generation

The phenology ofCapnodis tenebrionisadults was presented with reference to two different climate conditions. In a temperate moderate-warm climate, adult density showed two separate peaks during the year: one in early summer of the overwintering generation and one with beetles emerging in the late summer. In a warmer semiarid climate, the overwintering adults and the new generation overlapped during summer with a continuous increase of adult density. The difference in the average annual temperature between areas during the study period was almost3∘C, and, in the warmer area, the new generation ofC. tenebrionisemerged at least one month earlier. To make a prediction of adult presence, a model utilizing degree-days was developed from data collected over a five-year period. Models obtained from equations (Logistic 4-parameter,y(x)=yo+a/(1+(x/xo)b)) of each year were developed to describe the relationship between degree-day accumulation (with a minimal threshold activity temperature of14.21∘Ccalculated in the laboratory) and the cumulative percentage of adult presence. According to the overall model, the 50% of overwintering beetles occurred at 726 degree-days (Biofix: 1st March) and the emerging beetles occurred at 801 degree-days (Biofix: 1st July). The results show that a change in temperature is an important aspect that highlights the adaptability of this species.

Impact of extrafloral nectar availability and plant genotype on ant (Hymenoptera: Formicidae) visitation to quaking aspen (Salicaceae)

2015 ◽  
Vol 148 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Jonathon R. Newman ◽  
Diane Wagner ◽  
Patricia Doak
Keyword(s):  
Populus Tremuloides ◽  
Late Summer ◽  
Early Summer ◽  
Quaking Aspen ◽  
Plant Genotype ◽  
Free Living ◽  
Natural Induction ◽  
Formica Fusca ◽  
Time Of Year ◽  
Nectar Availability

AbstractFor quaking aspen (Populus tremuloides Michaux; Salicaceae) the rate of extrafloral (EF) sugar secretion is increased by defoliation and decreased by drought. Although wholesale blocking of EF nectar has been shown to reduce ant (Hymenoptera: Formicidae) visitation to aspen, the effect of more subtle and realistic variations in nectar availability on ant recruitment is unknown. Working in Alaskan boreal forest (United States of America), we reduced and supplemented EF nectar availability on potted aspen ramets of three genotypes and surveyed visitation by free-living Formica fusca (Linnaeus) (Hymenoptera: Formicidae). Ants were more responsive to a subtle increase in sugar availability than to a decrease. While nectar reduction had no effect on ant visitation, nectar supplementation increased ant visitation to one aspen genotype by 70% during an early summer trial. Average ant visitation to different aspen genotypes varied during the late summer, indicating that aspen genotype can influence attractiveness to ants. We conclude that natural induction of EF secretion in response to herbivory may benefit aspen through improved ant recruitment, though the response is dependent on aspen genotype and time of year. Differences among aspen genets in attractiveness to ants could influence the relative success of genotypes, especially in settings in which aspen regenerates from seed.

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