scholarly journals PHYTOCHEMICAL INVESTIGATION AND PHARMACOLOGICAL ACTIVITIES OF HELIOTROPIUM CURASSAVICUM LINN

2019 ◽  
Vol 49 (2) ◽  
pp. 105-109
Author(s):  
W. WASIULLAH ◽  
Saleem JAN ◽  
A. A. SHAD ◽  
A. BASIT ◽  
F. ULLAH
Keyword(s):  
Antioxidant Activity ◽  
Positive Control ◽  
Negative Control ◽  
Total Phenolic ◽  
Pharmacological Activities ◽  
Phenolic Contents ◽  
Phytochemical Investigation ◽  
In Vitro Antibacterial Activity ◽  
Pure Dmso

The current study was focused on the phytochemical investigation and pharmacological activities of the various fractions (methanolic crude, defatted methanolic, n-hexane, dichloromethane, ethylacetate and water) of H. curassavicum. The various plant extracts were examined for the total phenolic contents by taking Gallic acid as a standard, antioxidant scavenging assay by using DPPH (2, 2diphenyl-1-picrylhydrazy) & ABTS [2, 2-azinobis (3ethylbenzthiazoline-6-sulphonic acid)] free radicals, antifungal assay by using Terbinafine solution in DMSO as positive control and pure DMSO as negative control and in-vitro antibacterial activity. Methanolic crude extract showed high phenolic contents and significant antioxidant activity (both DPPH & ABTS). All other fractions were also found to have significant potential.

scholarly journals Phenolic Contents and Antioxidant Potential ofCrataegusFruits Grown in Tunisia as Determined by DPPH, FRAP, andβ-Carotene/Linoleic Acid Assay

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Farouk Mraihi ◽  
Mohamed Journi ◽  
Jamila Kalthoum Chérif ◽  
Munevver Sokmen ◽  
Atalay Sokmen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Frap Assay ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Total Anthocyanins ◽  
Linoleic Acid Assay

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

scholarly journals Actinidia macrospermaC. F. Liang (a Wild Kiwi): Preliminary Study of Its Antioxidant and Cytotoxic Activities

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Yin Lu ◽  
Xiangtao Du ◽  
Lidan Lai ◽  
Hao Jin
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Positive Control ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Etoac Extract ◽  
Total Antioxidant

The antioxidant potential ofActinidia macrospermaC. F. Liang (Actinidiaceae) was investigated in vitro for total phenolic content, along with total antioxidant activity (TAA), 1,1-diphenyl 2-picryl hydrazyl (DPPH), and lipid peroxidation (LP). The results indicated that different polarity extracts ofA. macrospermaexhibit different biological activities, which depends mainly on the presence of phenolic compounds. The antioxidant activity was in the following decreasing order: MeOH extract > EtOAc extract > aqueous extract > CHCl3extract > Hexane extract. Moreover, the cytotoxic activity of this plant by MTT dye assay using SMMC-7721 has been determined also. The hexane, EtOAc, and CHCl3extracts showed cytotoxicity in a dose-dependent manner. Methanol and aqueous extracts, however, showed weak activities in this test. And a very significant cytotoxic activity, not significantly different from the positive control of quercetin, was observed in CHCl3extract.

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

The Study of Flavonoids and Polysaccharide Content and Antioxidant Capacity In Vitro of Compound Chinese Herbal Medicine in Changbai Mountain

2014 ◽  
Vol 926-930 ◽  
pp. 1036-1039
Author(s):  
Yang Ting Zhang ◽  
Yan Li Xi ◽  
Min Liu ◽  
Hong Bo Ma
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Chemical Method ◽  
Positive Control ◽  
Negative Control ◽  
Equisetum Arvense ◽  
Chinese Herbal ◽  
Crataegus Pinnatifida

The compounds, which extracted from Equisetum arvense, Crataegus pinnatifida Bunge, Rosa maximowicziana Regel and Physalis alkekengi, were selected to determine the content of flavonoids and polysaccharides by a visible light spectrophotometry, and the antioxidant activity was determined by a chemical method. The results showed that the content of flavonoids in our compounds was 0.204 mg/g and the polysaccharides was 2.498 mg/g. In scavenging experiments, the levels of hydroxyl radicals and superoxide anion were lower compared with negative control, but the levels were still higher than positive control. The ability to scavenge superoxide anion was more powerful than hydroxyl radicals. This determined that our compound preparation extract have the ability to scavenge hydroxyl radicals and superoxide anion.

scholarly journals Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

2021 ◽  
Vol 33 (9) ◽  
pp. 2237-2243
Author(s):  
Pooja N. Akhand ◽  
Veena Sharma ◽  
Anupam K. Pathak
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Central India ◽  
Total Phenolic ◽  
Climatic Zones ◽  
Phenolic Contents ◽  
Total Antioxidant ◽  
Sphaeranthus Indicus

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

In vitro antioxidant and enzyme inhibitory properties and phenolic contents of crude extracts and fractions from different organs of the halophyte Lycium europaeum L.

2021 ◽  
Vol 17 ◽  
Author(s):  
Houaria Bendjedou ◽  
Malika Bennaceur ◽  
Houari Benamar ◽  
Maria João Rodrigues ◽  
Catarina Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Properties ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Reducing Activity ◽  
Ethanol Extracts ◽  
High Level ◽  
Β Carotene

Background: Lycium europaeum L. is a medicinal and edible Mediterranean halophyte spiny shrub. However, studies regarding its biological properties focused mainly on its aerial organs. Objective: The objective of the present work was to make a comparative evaluation of the in vitro antioxidant and inhibitory enzyme activities of ethanol extracts and fractions (chloroform, ethyl acetate, n-butanol and aqueous) from roots and leaves of L. europaeum, along with its total phenolic, flavonoid and tannin contents. Methods: The antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), superoxide radical, β-carotene bleaching, cupric reducing, and the ferric reducing activity methods. Results: The n-butanol fraction from roots had the highest antioxidant activity in all the assays. It was also the most active against acetylcholinesterase, butyrylcholinesterase, and urease (IC50 values 92.63, 118.26, and 135.60 µgmL-1, respectively). This fraction showed a high level of total phenolic, flavonoid, and tannin contents. Conclusion: The results suggest L. europaeum, especially its roots, as a candidate to be further explored as a source of bioactive products.

scholarly journals A Simple and a Reliable Method to Quantify Antioxidant Activity In Vivo

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 142 ◽  
Author(s):  
María Pilar de Torre ◽  
Rita Yolanda Cavero ◽  
María Isabel Calvo ◽  
José Luis Vizmanos
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Reliable Method ◽  
Positive Control ◽  
Negative Control ◽  
In Vivo Model ◽  
In Vivo Experiments ◽  
Age Related

The characterization of compounds with antioxidant activity is of great interest due to their ability to reduce reactive oxygen species production and, therefore, prevent some age-related diseases. Its antioxidant capacity can be analyzed by different methods both in vitro and in vivo. Caenorhabditis elegans is an in vivo model widely used in ageing research. Until now, available tests analyze functional effects in the worms, so the antioxidant activity of the compound is indirectly monitored. We have developed a simple and a reliable method to quantify internal antioxidant activity in vivo. To validate this method, we analyzed an aqueous green tea extract and two other compounds with a well-known antioxidant activity and without this activity. The results obtained (EC50 green tea = 21.76 ± 1.28 µg/mL; EC50 positive control = 8.50 ± 0.33 µg/mL; negative control EC50 > 500 µg/mL) can help in the design of further in vivo experiments. Thus, our method can be used as a previous screening capable of reducing the gap between in vitro and in vivo assays.

scholarly journals Evaluation of Total Flavonoid, Total Phenolic Contents, and Antioxidant Activity of Strychnobiflavone

2020 ◽  
Vol 20 (3) ◽  
pp. 716 ◽  
Author(s):  
Sana Ullah ◽  
Chang-Gu Hyun
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Antioxidant Agent ◽  
Increasing Demand ◽  
Phenolic And Flavonoid

This work evaluates the antioxidant activity of strychnobiflavone due to the increasing demand of the antioxidant agents day by day. Various in vitro antioxidants assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to investigate the antioxidant activity of strychnobiflavone. The results of both DPPH and ABTS show that strychnobiflavone increase the scavenging activity in a concentration-dependent manner due to the phenolic and flavonoid contents. This study revealed that strychnobiflavone is one of the promising and an effective compound for the antioxidant agent.

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