scholarly journals Epidemiological aspects, haematological and biochemical alterations in some gastrointestinal parasitic diseases of carnivores

2021 ◽  
Vol 26 (2) ◽  
pp. 9-16
Author(s):  
Marius Stelian Ilie ◽  
Roxana Gabriela Oanea ◽  
Mirela Imre ◽  
Iasmina Luca ◽  
Tiana Florea ◽  
...  
Keyword(s):  
Haemoglobin Concentration ◽  
Maximum Level ◽  
Gastrointestinal Parasites ◽  
Corpuscular Haemoglobin ◽  
Biochemical Alterations ◽  
Physiological Limits ◽  
Mean Corpuscular Haemoglobin ◽  
Physiological Values ◽  
Zoonotic Parasites ◽  
Pathogenic Agents

Gastrointestinal parasites are widespread pathogenic agents and one of the main causes for mortality in young dogs and cats. Many of these zoonotic parasites are relevant in terms of public health. The presence of parasites in the animal organism causes local and general modifications in the various organs they parasitize or transit throughout their life cycle. The present study aimed to identify the most frequent gastrointestinal parasites of dogs and cats and to monitor the alterations that occur in terms of haematological and biochemical parameters. The studied animals, 25 dogs and three cats from Timiș and Caraș Severin counties, were brought to the On-call room of the University Clinics of the Faculty of Veterinary Medicine Timișoara. The laboratory methods that were used were the Willis flotation method, the Baerman larvoscopic method and the Lugol method. The haematological methods, namely flow cytometry, cytochemistry and spectrophotometry, were performed at Bioclinica Laboratories, on whole blood samples that were collected in EDTA or simple tubes. The studied animals were positive for Giardia, Cystoisospora, Dipylidium, Ancylostoma, Toxocara and Trichocephalus. The positivity rate was 57.14%, with prevalence rates according to the parasitic species ranging from 3.57% to 21.42%, with multiparasitism in 32.14%, and monoparasitism in 17.85%. The values recorded for red blood cells, haemoglobin and hematocrit followed the same trendmost of the animals being situated within physiological values, except for three dogs, that recorded values below the minimal level. In the case of MCH (mean corpuscular haemoglobin) and MCHC (mean corpuscular haemoglobin concentration) the values recorded for most dogs were within physiological limits, except for three dogs which overpassed the maximum level. Eosinophils were high in all dogs, which is a characteristic feature of parasitism. The serum urea concentrations revealed the fact that all for dogs that were taken into study had values above the maximum limit.

scholarly journals HAEMATO-BIOCHEMICAL ALTERATIONS AS BIOMARKERS OF LEAD INDUCED TOXICITY IN MALE WISTAR RATS

2017 ◽  
Vol 14 (2) ◽  
pp. 227-232 ◽  
Author(s):  
B. S. Okediran ◽  
O. B. Kasali ◽  
S. O. Omotainse ◽  
Q. A. Akinloye
Keyword(s):  
Lead Acetate ◽  
Haemoglobin Concentration ◽  
Lead Toxicity ◽  
Male Rats ◽  
Oral Exposure ◽  
Corpuscular Haemoglobin ◽  
Biochemical Alterations ◽  
Total Plasma Protein ◽  
Mean Corpuscular Haemoglobin ◽  
Wistar Male Rats

The present work is devoted to access some of the biomarkers due to lead toxicity in experimental Wistar male rats orally exposed to graded doses of lead (200, 300 and 400 ppm of lead as lead acetate) over a period of four, eight and twelve weeks. A total of sixty Wistar male rats were equally divided into four groups A, B, C and D. Group A served as control. Groups B, C and D were exposed to 200, 300 and 400 ppm of lead as lead acetate respectively. At the end of four, eight and twelve weeks, five animals each were removed from each group; blood sample was obtained via the ocular median canthus for haematological and biochemical studies. The results indicated accumulation of blood lead which was both dose and time dependent, while there was significant decrease (P < 0.05) in packed cell volume and haemoglobin concentration. The erythrocyte indices (mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) also revealed a significant decrease, revealing microcytic hypochromic anaemia. There was significant decrease (P < 0.05) in both the total plasma protein and albumin while blood urea nitrogen and creatinine revealed a significant increase, giving an indication of compromised liver and kidney functions. It was concluded that oral exposure to lead at vary doses over a period of four, eight and twelve weeks results in alterations of various haematological and biochemical parameters and thus can serve as a sensitive biomarkers in lead toxicity.

scholarly journals Physiological values of some blood indicators in selected dwarf rabbit breeds

2017 ◽  
Vol 25 (1) ◽  
pp. 27 ◽  
Author(s):  
V. Šimek ◽  
D. Zapletal ◽  
E. Straková ◽  
A. Pavlík ◽  
P. Suchý
Keyword(s):  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Live Weight ◽  
Inorganic Phosphorus ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin Concentration ◽  
Red Blood Cell Count ◽  
Mean Corpuscular Haemoglobin ◽  
Physiological Values ◽  
Corpuscular Haemoglobin Concentration

<p>The aim of the present study was to evaluate the effect of breed on haematological and biochemical indicators in 3 dwarf rabbit breeds. In the experiment, 30 sexually intact dwarf rabbit females aged 6 mo were used. With the sole exception of white blood cells and haematocrit value, breed had the most significant effect on the majority of haematological indicators monitored. The red blood cell count was higher in the Dwarf Lop compared to the Netherland Dwarf (+1.91×10<sup>12</sup> cells/L; P&lt;0.05) and also the Teddy Dwarf (+1.32×10<sup>12</sup> cells/L; P&lt;0.05). For haemoglobin concentration, a higher value was found in the Netherland Dwarf than in the Teddy Dwarf (+39.29 g/L; P&lt;0.05) and the Dwarf Lop (+26.36 g/L; P&lt;0.05). For erythrocytic indicators, the highest values of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were found in the Netherland Dwarf. The breed had a significant effect on the urea and potassium values. A higher value of urea was recorded in the Dwarf Lop compared to the Teddy Dwarf (+1.56 mmol/L; P&lt;0.05). For potassium, a higher value was found in the Netherland Dwarf compared to the Teddy Dwarf (+0.85 mmol/L; P&lt;0.05). In addition, a significantly positive correlation (P&lt;0.05) was found between the live weight of dwarf females and values of haematocrit (0.49), albumin (0.54), alanine aminotransferase (0.51), and aspartate aminotransferase (0.41), while a significantly negative correlation (P&lt;0.05) was found between their live weight and values of triacylglycerols (–0.44), alkaline phosphatase (–0.38) and inorganic phosphorus (–0.52). </p>

scholarly journals Nitrite Intoxication of Common Carp (Cyprinus carpio L.) at Different Water Temperatures

2006 ◽  
Vol 75 (4) ◽  
pp. 561-569 ◽  
Author(s):  
H. Kroupová ◽  
J. Máchová ◽  
V. Piačková ◽  
M. Flajšhans ◽  
Z. Svobodová ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Haemoglobin Concentration ◽  
Nitrite Accumulation ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Higher Temperature ◽  
Lower Temperature ◽  
Plasma Nitrite

Common carp (Cyprinus carpio L.) were exposed to nitrite (1.45 mmol l-1 NO2-) for 48 hours at 14 °C and 20 °C, in order to investigate the mechanism of nitrite poisoning at these water temperatures. The effect of nitrite exposure on fish was assessed on selected haematological and biochemical indicators of the blood. Moreover, nitrite accumulation in the blood, liver and muscle was measured. Nitrite exposure produced high levels of methaemoglobin (88.2 ± 3.3% and 92.9 ± 6.1%) at both water temperatures compared with controls (0.3 ± 0.6% and 2.6 ± 3.0%). High fish mortality occurred in experimental groups (30% and 51%) compared with controls (0%). Nitrite exposure also resulted in an accumulation of nitrite in the fish body. The highest nitrite levels developed in the blood plasma, followed by the liver and muscle, respectively. Carp concentrated nitrite in the blood plasma and tissues to markedly higher levels at higher temperature (20 °C). The plasma nitrite concentrations (10.5 ± 1.9 mmol l-1) were in this case more than 7 times higher than the environmental one. At lower temperature (14 °C), plasma nitrite concentration reached 5.0 ± 1.5 mmol l-1. In either event, plasma K+ levels increased and Cl- levels and osmolality remained unchanged. Plasma Na+ levels slightly decreased at the higher temperature. Nitriteexposed fish showed lower haematocrit values (PCV) at both experimental temperatures compared with controls. At 20 °C, the blood haematocrit decrease (0.20 ± 0.02 l l-1) was accompanied by a low erythrocyte count (1.05 ± 0.12 1012 l-1) and by a low haemoglobin level (51 ± 11 g l-1). At the lower temperature (14 °C), the haematocrit decrease (0.25 ± 0.02 l l-1) was caused by a low mean corpuscular volume (167 ± 27 fl). No significant changes were observed in the mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), or selected erythrocyte dimensions (major axis, minor axis and aspect ratio).

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

Haematological Profiles of Pigs on Different Farms Reflect Their Health Status

2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj
Keyword(s):  
Health Status ◽  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell ◽  
Haemoglobin Concentration ◽  
Haematological Parameters ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.

IN VITRO EFFECT OF PHARMACEUTICAL EFFLUENT ON HAEMATOLOGY AND BIOCHEMICAL RESPONSES IN AFRICAN CATFISH (CLARIAS GARIEPINUS)

2021 ◽  
Vol 4 (4) ◽  
pp. 391-400
Author(s):  
Shafiu Nafiu Abdullahi ◽  
M. Zakariyya ◽  
Z. Y. Hassan ◽  
M. K. Ahmad
Keyword(s):  
Heavy Metals ◽  
Negative Impact ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Corpuscular Haemoglobin ◽  
Biochemical Alterations ◽  
Experimental Fish ◽  
Mean Corpuscular Haemoglobin ◽  
Vitro Effect

Pharmaceutical effluents collected from Bompai industrial area, Kano State were assessed for their physiochemical parameters, heavy metals and effect on haematology and biochemical changes in Clarias gariepinus. Laboratory analyses were performed using standard methods in a Completely Randomized Design (CRD). Five test solutions of the effluents (0, 25, 50, 75, and 100% v/v) were prepared for LC50 96hr acute toxicity test. The LC50 for 96hr was 48.7% concentration of effluents by volume. Experimental fish were exposed to sublethal concentrations of 0.00% 2.43% (5/100 LC50), 12.17% (25/100 LC50), 24.35% (50/100 LC50) and 36.52% (75/100 LC50) for 28days.  Physicochemical parameters recorded were higher than WHO recommended Standard with the exception of water temperature. Heavy metals concentrations decreased in the order of Cr > Cu > Pd > Cd. Red blood Cells count, haemoglobin concentrations, packed cell volume, lymphocytes and monocytes of the experimental fish decline significantly (p<0.05) when exposed with 2.43, 12.17, 24.35, 36.52% effluents  compared to the control. White blood cell count, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentrations, neutrophils and eosinophils were significantly higher (P<0.05) than the control. However, a significant increase (P<0.05) in the activities of serum aspartate aminotransferase (AST), Alkaline Phosphatase (ALP) and alanine aminotransferase (ALT) were recorded as the exposure period continued when compared with control. It is concluded that the effluents induces haematological and biochemical alterations. It is therefore recommended that regulatory bodies should adopt holistic approach on the aquatic pollution abatement, bearing in mind the negative impact to non-target organisms.

Ecotoxicological assessment of commercial glyphosate-based di-ammonium salt formulation on haematological indices of Clarias gariepinus

2021 ◽  
Vol 36 (1) ◽  
pp. 13-21
Author(s):  
A.B. Ella ◽  
E.T. Azua ◽  
C.U. Aguoru ◽  
A.A. Onekutu ◽  
F.A. Ella
Keyword(s):  
Blood Cell ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
Negative Consequences ◽  
Ecotoxicological Assessment ◽  
Corpuscular Haemoglobin ◽  
Physico Chemical ◽  
Haematological Profile ◽  
Mean Corpuscular Haemoglobin ◽  
Total Dissolved Solid

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different  concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.

scholarly journals The Haematology and Serum Biochemistry Profile of Adult Japanese Quail (Coturnix coturnix japonica)

2017 ◽  
Vol 9 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Onyinyechukwu A. AGINA ◽  
Wilfred S. EZEMA ◽  
Ezinwa M. IWUOHA
Keyword(s):  
Uric Acid ◽  
Total Cholesterol ◽  
Haemoglobin Concentration ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Serum Biochemistry ◽  
Total Proteins ◽  
Corpuscular Haemoglobin ◽  
Coturnix Coturnix ◽  
Mean Corpuscular Haemoglobin

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.

scholarly journals Values of Selected Haematological Indicators in Six Species of Feathered Game

2010 ◽  
Vol 79 (9) ◽  
pp. S3-S8 ◽  
Author(s):  
Eva Straková ◽  
Pavel Suchý ◽  
Romana Kábelová ◽  
František Vitula ◽  
Ivan Herzig
Keyword(s):  
Blood Cell ◽  
Total Concentration ◽  
Haemoglobin Concentration ◽  
Individual Species ◽  
Neutrophil Granulocytes ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin Concentration ◽  
Mean Corpuscular Haemoglobin ◽  
The Mean ◽  
Corpuscular Haemoglobin Concentration

The present paper extends the knowledge of selected blood indicators in six species of feathered game such as helmeted guineafowl (Numida meleagris), common pheasant (Phasianus colchicus), Japanese quail (Coturnix coturnix japonica), chukar (Alectoris chucar), grey partridge (Perdix perdix) and wild turkey (Meleagris gallopavo), reared in the Czech Republic and Europe. The red blood cell (RBC) values in individual species of feathered game ranged as follows: the mean RBC counts varied in a range of 2.10 - 2.58 T/l; haematocrit values were in a range of 0.33 - 0.39 1/1; haemoglobin concentration varied in a range of 101.20 - 129.13 g/l; mean corpuscular haemoglobin concentration (MCHC) ranged from 28.63 to 35.44%; mean corpuscular haemoglobin (MCH) varied in a range of 46.15 - 62.07 pg and the mean corpuscular volume (MCV) was 147.66 - 178.20 fl. Except for the total concentration of haemoglobin and the mean corpuscular haemoglobin concentration, no significant differences were found between the mean values of RBC values in individual species of feathered game. However, some differences between species were found in white blood cell (WBC) values, ranging as follows: the total WBC count ranged from 13.55 to 26.90 G/l, basophil granulocytes from 0.011 to 0.110 G/l, eosinophil granulocytes from 0.011 to 0.593 G/l, neutrophil granulocytes from 1.868 to 6.661 G/l, lymphocytes from 10.937 to 20.922 G/l and monocytes from 0.034 to 0.199 G/l. Most values showed significant (P ⪬ 0.05) to highly significant (P ⪬ 0.01) interspecies differences. The main goal of the study was to investigate selected haematological indicators in six major species of feathered game.

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