HAEMATO-BIOCHEMICAL ALTERATIONS AS BIOMARKERS OF LEAD INDUCED TOXICITY IN MALE WISTAR RATS

The present work is devoted to access some of the biomarkers due to lead toxicity in experimental Wistar male rats orally exposed to graded doses of lead (200, 300 and 400 ppm of lead as lead acetate) over a period of four, eight and twelve weeks. A total of sixty Wistar male rats were equally divided into four groups A, B, C and D. Group A served as control. Groups B, C and D were exposed to 200, 300 and 400 ppm of lead as lead acetate respectively. At the end of four, eight and twelve weeks, five animals each were removed from each group; blood sample was obtained via the ocular median canthus for haematological and biochemical studies. The results indicated accumulation of blood lead which was both dose and time dependent, while there was significant decrease (P < 0.05) in packed cell volume and haemoglobin concentration. The erythrocyte indices (mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) also revealed a significant decrease, revealing microcytic hypochromic anaemia. There was significant decrease (P < 0.05) in both the total plasma protein and albumin while blood urea nitrogen and creatinine revealed a significant increase, giving an indication of compromised liver and kidney functions. It was concluded that oral exposure to lead at vary doses over a period of four, eight and twelve weeks results in alterations of various haematological and biochemical parameters and thus can serve as a sensitive biomarkers in lead toxicity.

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Epidemiological aspects, haematological and biochemical alterations in some gastrointestinal parasitic diseases of carnivores

Cluj Veterinary Journal ◽

10.52331/cvj.v26i2.25 ◽

2021 ◽

Vol 26 (2) ◽

pp. 9-16

Author(s):

Marius Stelian Ilie ◽

Roxana Gabriela Oanea ◽

Mirela Imre ◽

Iasmina Luca ◽

Tiana Florea ◽

...

Keyword(s):

Haemoglobin Concentration ◽

Maximum Level ◽

Gastrointestinal Parasites ◽

Corpuscular Haemoglobin ◽

Biochemical Alterations ◽

Physiological Limits ◽

Mean Corpuscular Haemoglobin ◽

Physiological Values ◽

Zoonotic Parasites ◽

Pathogenic Agents

Gastrointestinal parasites are widespread pathogenic agents and one of the main causes for mortality in young dogs and cats. Many of these zoonotic parasites are relevant in terms of public health. The presence of parasites in the animal organism causes local and general modifications in the various organs they parasitize or transit throughout their life cycle. The present study aimed to identify the most frequent gastrointestinal parasites of dogs and cats and to monitor the alterations that occur in terms of haematological and biochemical parameters. The studied animals, 25 dogs and three cats from Timiș and Caraș Severin counties, were brought to the On-call room of the University Clinics of the Faculty of Veterinary Medicine Timișoara. The laboratory methods that were used were the Willis flotation method, the Baerman larvoscopic method and the Lugol method. The haematological methods, namely flow cytometry, cytochemistry and spectrophotometry, were performed at Bioclinica Laboratories, on whole blood samples that were collected in EDTA or simple tubes. The studied animals were positive for Giardia, Cystoisospora, Dipylidium, Ancylostoma, Toxocara and Trichocephalus. The positivity rate was 57.14%, with prevalence rates according to the parasitic species ranging from 3.57% to 21.42%, with multiparasitism in 32.14%, and monoparasitism in 17.85%. The values recorded for red blood cells, haemoglobin and hematocrit followed the same trendmost of the animals being situated within physiological values, except for three dogs, that recorded values below the minimal level. In the case of MCH (mean corpuscular haemoglobin) and MCHC (mean corpuscular haemoglobin concentration) the values recorded for most dogs were within physiological limits, except for three dogs which overpassed the maximum level. Eosinophils were high in all dogs, which is a characteristic feature of parasitism. The serum urea concentrations revealed the fact that all for dogs that were taken into study had values above the maximum limit.

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Zinc ameliorates lead toxicity by reducing body Pb burden and restoring Pb-induced haematological and biochemical derangements

Toxicology Research and Application ◽

10.1177/2397847320956562 ◽

2020 ◽

Vol 4 ◽

pp. 239784732095656

Author(s):

Emmanuel Ike Ugwuja ◽

Nweze Vincent ◽

Ikechukwu C Ikaraoha ◽

Samuel R Ohayi

Keyword(s):

Blood Cell ◽

Cell Count ◽

Haemoglobin Concentration ◽

Lead Toxicity ◽

Oral Route ◽

Albino Rats ◽

Blood Cell Count ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin ◽

Biochemical Analyses

Background: Studies on nutritional factors, including zinc in ameliorating the deleterious effects of potentially toxic elements have been scarce and the findings have been inconsistent. Aim: The aim of the present study was to evaluate the ameliorative potential of zinc against lead-induced toxicity in rats. Materials and methods: Male albino rats (n = 24) assigned into four groups of six/group: normal control (NC), zinc control (ZnC; 20 mg/kg ZnCl2), lead control (PbC; 50 mg/kg PbCl2) and lead plus zinc (Pb + Zn; 50 mg/kg PbCl2 plus 20 mg/kg ZnCl2), respectively were investigated. All administrations were through oral route and lasted for 42 days after which blood samples were collected for haematological and biochemical analyses using standard techniques. Results: Results showed that packed cell volume (PCV), haemoglobin concentration (HBC), red blood cell count (RBC) were significant (p < 0.05) reduced while total white blood cell count (TWBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets were significantly elevated in PbC group in comparison with NC and ZnC. Also liver and renal function parameters as well as the liver and kidney malondialdehyde (MDA) were elevated while antioxidant enzymes; catalase (CAT) and superoxide dismutase (SOD) were significantly (p < 0.05) reduced in PbC group in comparison with NC and ZnC. Concentrations of lead were in the order: blood > liver > kidney in the PbC group. In addition to restoration of the altered parameters, administration of zinc in Pb + Zn group significantly reduced the raised lead concentrations in the plasma and organs. Conclusion: Reduction in body Pb burden and restoration of Pb-induced derangements in haematological and biochemical parameters by Zn strongly support the ameliorative property of Zn against Pb-induced toxicity.

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Alterations in Some Haematological Parameters of The African Snakehead: Parachanna africans Exposed to Cadmium

Notulae Scientia Biologicae ◽

10.15835/nsb346299 ◽

2011 ◽

Vol 3 (4) ◽

pp. 29-34 ◽

Cited By ~ 4

Author(s):

Ovie KORI-SAKPERE ◽

Urowoli IKOMI

Keyword(s):

Blood Cell ◽

Haemoglobin Concentration ◽

Sublethal Concentration ◽

Haematological Parameters ◽

Corpuscular Haemoglobin ◽

Total Plasma Protein ◽

Mean Corpuscular Haemoglobin ◽

The Mean ◽

Wbc Count ◽

Hb Concentration

The haematological alterations produced on exposure of the African snakehead, Parachanna africans to the sublethal concentration (0.0, 0.1, 1.0 and 10.0 mg/L) of cadmium (Cd2+) for 21 days have been studied. Red blood cell (RBC) count, haemoglobin (Hb) concentration, haematocrit (Hct), the mean corpuscular haemoglobin (MCH) and the mean corpuscular volume (MCV) levels were decreased with an increase in exposure concentration, but the level of the mean corpuscular haemoglobin concentration (MCHC) was increased. These alterations could be attributed to haemolysis and impairment of haemoglobin synthesis, resulting in a hypochromic microcytic anaemia, induced by exposure to cadmium. Cadmium exposure also caused significant decreases in white blood cell (WBC) count. The primary consequence of the observed changes in total and differential leucocyte counts in stressed fish was attributed to suppression of the immune system and increased susceptibility to disease. Plasma glucose and total plasma protein concentrations were significantly decreased; showing that cadmium affects the fish energy metabolism. The present study thus confirmed that haematological parameters are very sensitive indicators of fish organism response to chemicals in this case cadmium.

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Nitrite Intoxication of Common Carp (Cyprinus carpio L.) at Different Water Temperatures

Acta Veterinaria Brno ◽

10.2754/avb200675040561 ◽

2006 ◽

Vol 75 (4) ◽

pp. 561-569 ◽

Cited By ~ 6

Author(s):

H. Kroupová ◽

J. Máchová ◽

V. Piačková ◽

M. Flajšhans ◽

Z. Svobodová ◽

...

Keyword(s):

Blood Plasma ◽

Common Carp ◽

Cyprinus Carpio ◽

Haemoglobin Concentration ◽

Nitrite Accumulation ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin ◽

Higher Temperature ◽

Lower Temperature ◽

Plasma Nitrite

Common carp (Cyprinus carpio L.) were exposed to nitrite (1.45 mmol l-1 NO2-) for 48 hours at 14 °C and 20 °C, in order to investigate the mechanism of nitrite poisoning at these water temperatures. The effect of nitrite exposure on fish was assessed on selected haematological and biochemical indicators of the blood. Moreover, nitrite accumulation in the blood, liver and muscle was measured. Nitrite exposure produced high levels of methaemoglobin (88.2 ± 3.3% and 92.9 ± 6.1%) at both water temperatures compared with controls (0.3 ± 0.6% and 2.6 ± 3.0%). High fish mortality occurred in experimental groups (30% and 51%) compared with controls (0%). Nitrite exposure also resulted in an accumulation of nitrite in the fish body. The highest nitrite levels developed in the blood plasma, followed by the liver and muscle, respectively. Carp concentrated nitrite in the blood plasma and tissues to markedly higher levels at higher temperature (20 °C). The plasma nitrite concentrations (10.5 ± 1.9 mmol l-1) were in this case more than 7 times higher than the environmental one. At lower temperature (14 °C), plasma nitrite concentration reached 5.0 ± 1.5 mmol l-1. In either event, plasma K+ levels increased and Cl- levels and osmolality remained unchanged. Plasma Na+ levels slightly decreased at the higher temperature. Nitriteexposed fish showed lower haematocrit values (PCV) at both experimental temperatures compared with controls. At 20 °C, the blood haematocrit decrease (0.20 ± 0.02 l l-1) was accompanied by a low erythrocyte count (1.05 ± 0.12 1012 l-1) and by a low haemoglobin level (51 ± 11 g l-1). At the lower temperature (14 °C), the haematocrit decrease (0.25 ± 0.02 l l-1) was caused by a low mean corpuscular volume (167 ± 27 fl). No significant changes were observed in the mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), or selected erythrocyte dimensions (major axis, minor axis and aspect ratio).

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Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2020.2.5.133 ◽

2020 ◽

Vol 2 (5) ◽

Author(s):

Y. Garba ◽

E. A. Adeola

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Serum Biochemical Parameters ◽

Corpuscular Haemoglobin ◽

Randomized Design ◽

Serum Biochemical ◽

Mean Corpuscular Haemoglobin ◽

Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

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Haematological Profiles of Pigs on Different Farms Reflect Their Health Status

10.21203/rs.3.rs-644732/v1 ◽

2021 ◽

Author(s):

Irena Golinar Oven ◽

Alenka Nemec Svete ◽

Melita Hajdinjak ◽

Jan Plut ◽

Marina Stukelj

Keyword(s):

Health Status ◽

Blood Cell ◽

Cell Count ◽

White Blood Cell ◽

Haemoglobin Concentration ◽

Haematological Parameters ◽

Blood Cell Count ◽

Blood Samples ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.

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IN VITRO EFFECT OF PHARMACEUTICAL EFFLUENT ON HAEMATOLOGY AND BIOCHEMICAL RESPONSES IN AFRICAN CATFISH (CLARIAS GARIEPINUS)

FUDMA Journal of Sciences ◽

10.33003/fjs-2020-0404-495 ◽

2021 ◽

Vol 4 (4) ◽

pp. 391-400

Author(s):

Shafiu Nafiu Abdullahi ◽

M. Zakariyya ◽

Z. Y. Hassan ◽

M. K. Ahmad

Keyword(s):

Heavy Metals ◽

Negative Impact ◽

Clarias Gariepinus ◽

African Catfish ◽

Corpuscular Haemoglobin ◽

Biochemical Alterations ◽

Experimental Fish ◽

Mean Corpuscular Haemoglobin ◽

Vitro Effect

Pharmaceutical effluents collected from Bompai industrial area, Kano State were assessed for their physiochemical parameters, heavy metals and effect on haematology and biochemical changes in Clarias gariepinus. Laboratory analyses were performed using standard methods in a Completely Randomized Design (CRD). Five test solutions of the effluents (0, 25, 50, 75, and 100% v/v) were prepared for LC50 96hr acute toxicity test. The LC50 for 96hr was 48.7% concentration of effluents by volume. Experimental fish were exposed to sublethal concentrations of 0.00% 2.43% (5/100 LC50), 12.17% (25/100 LC50), 24.35% (50/100 LC50) and 36.52% (75/100 LC50) for 28days. Physicochemical parameters recorded were higher than WHO recommended Standard with the exception of water temperature. Heavy metals concentrations decreased in the order of Cr > Cu > Pd > Cd. Red blood Cells count, haemoglobin concentrations, packed cell volume, lymphocytes and monocytes of the experimental fish decline significantly (p<0.05) when exposed with 2.43, 12.17, 24.35, 36.52% effluents compared to the control. White blood cell count, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentrations, neutrophils and eosinophils were significantly higher (P<0.05) than the control. However, a significant increase (P<0.05) in the activities of serum aspartate aminotransferase (AST), Alkaline Phosphatase (ALP) and alanine aminotransferase (ALT) were recorded as the exposure period continued when compared with control. It is concluded that the effluents induces haematological and biochemical alterations. It is therefore recommended that regulatory bodies should adopt holistic approach on the aquatic pollution abatement, bearing in mind the negative impact to non-target organisms.

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Ecotoxicological assessment of commercial glyphosate-based di-ammonium salt formulation on haematological indices of Clarias gariepinus

Journal of Aquatic Sciences ◽

10.4314/jas.v36i1.2 ◽

2021 ◽

Vol 36 (1) ◽

pp. 13-21

Author(s):

A.B. Ella ◽

E.T. Azua ◽

C.U. Aguoru ◽

A.A. Onekutu ◽

F.A. Ella

Keyword(s):

Blood Cell ◽

Clarias Gariepinus ◽

Haemoglobin Concentration ◽

Negative Consequences ◽

Ecotoxicological Assessment ◽

Corpuscular Haemoglobin ◽

Physico Chemical ◽

Haematological Profile ◽

Mean Corpuscular Haemoglobin ◽

Total Dissolved Solid

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.

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The Haematology and Serum Biochemistry Profile of Adult Japanese Quail (Coturnix coturnix japonica)

Notulae Scientia Biologicae ◽

10.15835/nsb919928 ◽

2017 ◽

Vol 9 (1) ◽

pp. 67-72 ◽

Cited By ~ 2

Author(s):

Onyinyechukwu A. AGINA ◽

Wilfred S. EZEMA ◽

Ezinwa M. IWUOHA

Keyword(s):

Uric Acid ◽

Total Cholesterol ◽

Haemoglobin Concentration ◽

Sampling Technique ◽

Simple Random Sampling ◽

Serum Biochemistry ◽

Total Proteins ◽

Corpuscular Haemoglobin ◽

Coturnix Coturnix ◽

Mean Corpuscular Haemoglobin

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.

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