Colonization Frequency, Endophytic Infection Rate and Bioactivities of Microbes of Desert Medicinal Plants

2019 ◽  
Vol 41 (3) ◽  
pp. 501-501
Author(s):  
Suriya Rehman Suriya Rehman ◽  
Mohammad Azam Ansari Mohammad Azam Ansari ◽  
Abdullah Buhaimed Abdullah Buhaimed ◽  
Fatemah Ibrahim and Adil Gani Fatemah Ibrahim and Adil Gani
Keyword(s):  
Medicinal Plants ◽  
Infection Rate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Gram Positive ◽  
Gram Negative ◽  
Colonization Frequency

The present study, provides an information on the colonization of endophytic fungi in three desert medicinal plants, i.e. Artemesia sieberii, Citrillus colocynthis and Moringa peregrine, along with their biological activities. The highest colonization frequency (CF) and endophytic infection rate (EIR), was observed in C colocynthis (87.5% andamp; 81.25%), respectively, followed by A. sieberii (62.5 andamp; 56.25%) and M. peregrine (37.5 andamp; 31.25%), respectively. The colonization frequency was highest in leaves, when compared to the stem parts. Among the screened isolates, three selected endophytes, (ART 7) from A. sieberii, (CTC11) from C colocynthis and (MOP5) from M. peregrine, with the zone of inhibition greater than 12 mm, were further evaluated for minimum inhibitory concentration (MIC). MIC against gram positive was ranging from 125 to 1000 and#181;g/ml and 250 to 2000 and#181;g/ml against gram negative. Thus, gram-positive strains, were found to be more susceptible. CTC11 from C. colocynthis showed fairly better MIC, against both gram positive and gram-negative organisms, ranging from 125 to 500 and#181;g/ml and 250 to 1000 and#181;g/ml, respectively. The total phenolic content was determined, (ART 7) from A. sieberii, was found to possess a high phenolic content (12.6 and#177; 0.16). Therefore, the significant antimicrobial activity, reducing power and total phenolic content of the isolates, suggests the use of endophytes of desert medicinal plants, as a rich and reliable source of industrially important compounds.

scholarly journals An Evaluation of the Antioxidant Status and Antimicrobial Activity of the Methanol Extract of Ocimum Gratissimum

2016 ◽  
Vol 5 (1) ◽  
pp. 40
Author(s):  
Sajini Souda ◽  
George Saramma ◽  
Irene Goercke ◽  
Kelvin Chabaesele ◽  
Naledi Mannathoko
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Antioxidant Status ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Gram Positive ◽  
Gram Negative ◽  
Ocimum Gratissimum

<div><p><em>Herbal drug therapy is regarded as an important alternative for the treatment of chronic and infectious disease. Ocimum gratissimum has been used extensively in traditional medicine in many countries. The aim of this study was to evaluate antioxidant status and antimicrobial activity of the methanol extract of Ocimum gratissimum (MEOG) and to establish that the antimicrobial activity is due to its antioxidant potentials. The total antioxidant status was evaluated using DPPH and ABTS assays and the Folin-Ciocalteu reagent method to determine total phenolic content of the MEOG. The antimicrobial effect was determined by agar diffusion and broth dilution method against different ATCC strains of pathogenic bacteria. Zones of inhibition, minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) were determined.  The results indicate that the antioxidant potential and antimicrobial activity of MEOG is mainly due to its total phenolic content and bioactive ingredients like  tannins, flavonoids, saponins, phenols, quinones and terpenoids. The MEOG was found microbicidal against all tested gram positive and negative organisms and against the fungus, Candida albicans. The MIC for gram positive organisms ranged between 0.117 – 7.5mg/ml and for gram negative organisms the range were between 0.469 – 3.75 mg/ml. The MBC for the gram positive bacteria ranged from 0.117mg/ml – 15 mg/ml and for gram negative bacteria from 3.75 mg/ml- 15 mg/ml. In conclusion, MEOG can be used in the treatment of skin infections due to bacteria and fungus and also in the respiratory and gastro intestinal infections due to its antioxidant properties.<strong></strong></em></p></div>

scholarly journals Comparison of biochemical and antimicrobial activities of different honey samples

2021 ◽  
Vol 39 (No. 4) ◽  
pp. 273-280
Author(s):  
Saban Keskin ◽  
Semiramis Karlidag ◽  
Nazlı Mayda ◽  
Asli Ozkok
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Biological Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Total Phenolic ◽  
Honey Sample ◽  
Gram Positive ◽  
Gram Positive Bacteria

Honey, a natural healing agent and a sweet food, has been used since ancient times. A honey sample could possess many biological activities depending on its chemical composition. The amount and the diversity of these minor components of honey mainly depend on the floral sources. That is why the biological activity of the honey sample obtained in a region should be determined. In this study, total phenolic and flavonoid content, antioxidant activity, melissopalynological analyses and antimicrobial activity of twenty honey samples obtained from Doganyol, Malatya, Turkey were examined. In order to determine the in vitro antibacterial activity of honey samples, the agar well diffusion (AWD) method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were used. For this purpose, ten Gram-positive bacteria and eight Gram-negative bacteria were used. Total phenolic content was found in the range from 9.68 ± 0.72 to 29.40 ± 1.03 mg GAE g<sup>–1</sup> sample. Antioxidant activity of honey samples varied from 2.21 ± 0.46 to 6.03 ± 1.11 µmol FeSO<sub>4</sub> 7H<sub>2</sub>O g<sup>–1</sup>. Honey samples showed moderate antimicrobial activity against tested microorganisms, especially against Gram-positive bacteria. It could be concluded from our findings that there is a good correlation between total phenolic content and the biological activity of honey samples.

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

Evaluation of Antioxidant Properties, Total Phenolic Content, and Biological Activities of New Tomato Hybrids of Industrial Interest

2012 ◽  
Vol 15 (5) ◽  
pp. 483-489 ◽  
Author(s):  
Giuseppina Tommonaro ◽  
Rocco de Prisco ◽  
Gennaro Roberto Abbamondi ◽  
Stefania Marzocco ◽  
Carmela Saturnino ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Total Phenolic

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

scholarly journals Evaluation of Total Phenolic Content in Relation to Antioxidant Activity of Brown Algae Cystoseira barbata from Black Sea

2019 ◽  
Vol 70 (7) ◽  
pp. 2684-2689
Author(s):  
Emin Cadar ◽  
Rodica Sirbu ◽  
Alef Ibram ◽  
Ana Maria Ionescu
Keyword(s):  
Antioxidant Activity ◽  
Benzoic Acid ◽  
Black Sea ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Reducing Power ◽  
The Black Sea ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Cystoseira Barbata

Seaweeds are widely used in the life science as source of compounds with diverse structural forms and biological activities, therefore, potential source of new antioxidant activity. The algae of the Black Sea have not been adequately explored for their potential as a source of bioactive substances. In this context, Brown algae Cystoseira barbata isolated from Black Sea coastal waters of Romania, were evaluated for their bioactivity potential. The results from Cystoseira barbata were obtained: for total phenolic content obtained in ethanolic extracts (TPC): 385.6 � 1.85 mg GAE/100 g f.w. Phenolic Acids quantified by HPLC-DAD from hydroethanolic extract of Cystoseira barbata from Black Sea was 80.14% from total phenolic content identified. We showed that Cystoseira barbata from the Romanian shore of the Black Sea contains vanillic acid in highest quantity (99.5�0.08 mg/100 g f.w.), followed by benzoic acid (65.7�0.06 mg/100 g f.w.) and feluric acid (54.5�0.01 mg/100 g f.w.). The smallest quantities were for gallic acid (3.5�0.03 mg/100 g f.w.), pyrogallol acid (4.2�0.05 mg/100 g f.w.) and 4-amino-benzoic acid (5.2�0.09 mg/100 g f.w.). Cystoseira barbata has a total content of large phenols and also has strong antioxidant and antimicrobial activity. Antioxidant activity (ACL) was studied using DPPH radical scavenging activity and reducing power. The extracts containing high levels of total phenolic content were also potent DPPH radical scavengers. The IC50 (�g/mL) for Cystoseira barbata in methanolic extract was 942.92� 2.56 �g/mL. High absorbance obtained from reducing power assay indicates high reducing power, measured values of absorbance varied from 0.0901 at 0.9223.

scholarly journals Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Weihuan Huang ◽  
Joo-Shin Kim ◽  
Hau Yin Chung
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Shiitake Mushroom ◽  
Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

Chemical components and biological activities of two freshwater green algae from Ordu, Turkey / Türkiye’nin Ordu ilinden iki tatlı su yeşil alglerinin kimyasal bileşenleri ve biyolojik aktiviteleri

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Beyhan Taş ◽  
Ömer Ertürk ◽  
Özlem Yılmaz ◽  
Melek Çol Ayvaz ◽  
Emine Yurdakul Ertürk
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Algal Species ◽  
Acetone Extract ◽  
Diethyl Ester ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Green Algal

AbstractObjective: Scientists are looking for new resources which have biological activities. The present study was planned to evaluate the antioxidant and antimicrobial activity of ethanol and acetone extracts, as well as the volatile compounds of two freshwater green algal species Spirogyra spp. and Zygnema stellinum (Vaucher) C. Agardh belonging to Zygnemaphyceae (Conjugatophyceae) obtained from Ordu University Campus wetlands.Methods: The extracts were tested in vitro for their antimicrobial effects using disc diffusion method. Total phenolic content and the antioxidative activity according to FRAP and DPPH methods of the extracts were also determined. The secondary metabolites from the investigated extracts were identified using GC-MS.Results: The extracts dramatically inhibited almost all tested microorganisms. The antimicrobial activity of the acetone extract of the Zygnema against C. albicans was found as more higher than positive control Nystatin. In accordance with antimicrobial activity, the highest total phenolic content was also determined in the presence of the acetone extract of the Zygnema. Furthermore the highest FRAP value and the lowest EC50 (mg/mL) value were calculated for the same extract. The main components of the all consisted of dimethyl and diethyl ester of 1,2-benzenedicarboxylic acid. However, the abundance of these metabolites in the extracts was not associated with antimicrobial or antioxidant activity. Biological activities of these algal species could be attributed to chemicals such as 1-Pentadecene and 1-Tetradecene which were present in smaller amounts.Conclusion: Investigated algal species can be evaluated to use in biotechnological applications such as food industry and medicine.

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