Optimization of the parameters of extraction of root cultures

Biologically Active ◽

Scutellaria Baicalensis ◽

Extraction Temperature ◽

Root Cultures ◽

Optimal Parameters ◽

Настоящая работа затрагивает тему оптимизации процесса экстракции биологически активных веществ из биомассы корневых культур in vitro лекарственных растений Сибирского федерального округа, а именно шлемника байкальского, шлемника обыкновенного и лапчатки белой. Параметры, которые влияли на ход процесса, были выбраны следующие: продолжительность процесса, температура экстракции и гидромодуль. В ходе эксперимента выяснилось, что для максимального выхода экстракта шлемника байкальского необходимо соблюдать следующие параметры: соотношение объема растворителя, наиболее эффективного в данном случае 70 %-ного этанола, к массе исходного сырья 1:10, продолжительность процесса 60 мин, температура 50 °С. Максимальный выход биологически активных веществ из шлемника обыкновенного будет достигаться при температуре экстракции 60 °С в течение 60 мин с использованием 70 %-ного этанола в качестве органического растворителя в соотношении к высушенному образцу 1:10. Оптимальные параметры извлечения активных веществ из лапчатки белой: соотношение экстрагента, в качестве которого выступает диэтиловый эфир, к исходному сырью 1:20, продолжительность 60 мин при температуре экстракции 40 °С. Извлечение экстрактов из культур in vitro позволит сохранить популяцию растений, произрастающих в естественных условиях. This work touches on the topic of optimization of the process of extraction of biologically active substances from the biomass of root cultures in vitro of medicinal plants of the Siberian Federal District, namely Scutellaria galericulata, Scutellaria Baicalensis, Potentilla alba . The parameters that influenced the course of the process were chosen as follows: process duration, extraction temperature and hydronic module. In the course of the experiment, it turned out that for the maximum yield of the Scutellaria Baicalensis extract, the following parameters must be observed: the ratio of the volume of the solvent, the most effective in this case 70% ethanol, to the weight of the feedstock 1:10, the duration of the process is 60 min, the temperature is 50 °C. The maximum yield of biologically active substances from Scutellaria galericulata will be achieved at an extraction temperature of 60 °C for 60 minutes using 70 % ethanol as an organic solvent in a ratio of 1:10 to the dried sample. The optimal parameters for the extraction of active substances from Potentilla alba are: the ratio of the extractant, which is diethyl ether, to the feedstock 1:20, lasting 60 minutes at an extraction temperature of 40 °C. The extraction of extracts from in vitro cultures will allow preserving the population of plants growing in natural conditions.

Determination of the Qualitative Composition of Biologically Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhaponticum carthamoides

Applied Sciences ◽

10.3390/app11062555 ◽

2021 ◽

Vol 11 (6) ◽

pp. 2555

Author(s):

Lyudmila Asyakina ◽

Svetlana Ivanova ◽

Alexander Prosekov ◽

Lyubov Dyshlyuk ◽

Evgeny Chupakhin ◽

...

Keyword(s):

Medicinal Plant ◽

Cell Suspension ◽

Biologically Active ◽

Root Cultures ◽

Qualitative Composition ◽

Callus Cell ◽

Rhaponticum Carthamoides ◽

This work aims to study the qualitative composition of biologically active substance (BAS) extracts in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides. The research methodology is based on high-performance liquid chromatography, and 1H nuclear magnetic resonance (NMR) spectra, to study the qualitative and quantitative analysis of BAS. The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension and root cultures showed that the main biologically active substances in the medicinal plant Rhaponticum carthamoides are 2-deoxy-5,20,26-trihydroxyecdyson (7 mg, yield 0.12%), 5,20,26-trihydroxyecdyson 20,22-acetonide (15 mg, yield 0.25%), 2-deoxy-5,20,26-trihydroxyecdyson 20,22-acetonide (6 mg, yield 0.10%), 20,26-dihydroxyecdyson 20,22-acetonidecdyson 20,22-acetonide (5 mg, yield 0.09%), and ecdyson 20,22-acetonide (6 mg, yield 0.10%). In the future, it is planned to study the antimicrobial, antioxidant, and antitumor activity of BAS of extracts of in vitro callus, cell suspension, and root cultures of the medicinal plant Rhaponticum carthamoides, for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial and antioxidant effects.

Panax ginseng callus, suspension, and root cultures: extraction and qualitative analysis

Foods and raw materials ◽

10.21603/2308-4057-2020-2-369-376 ◽

2020 ◽

Vol 8 (2) ◽

pp. 369-376

Author(s):

Lyubov Dyshlyuk ◽

Anastasia Dmitrieva ◽

Svetlana Ivanova ◽

Yuliya Golubtsova ◽

Lev Ostroumov

Keyword(s):

Qualitative Analysis ◽

Organic Compounds ◽

Panax Ginseng ◽

Callus Cultures ◽

Biologically Active ◽

Root Cultures ◽

Optimal Parameters ◽

Promising Source ◽

Introduction. In recent years, scientists have been actively searching for medicinal plants containing biologically active substances with geroprotective properties to treat diseases of old age, in particular cancer, diabetes, cardiovascular diseases, and others. Ginseng (Panax ginseng L.) is a promising source of geroprotective compounds. We aimed to select optimal parameters for extracting organic compounds from ginseng callus, suspension, and root cultures and analyze their qualitative composition. Study objects and methods. We studied ginseng callus, suspension, and root cultures, as well as their extracts. Biologically active substances were extracted with 30 to 70% ethanol. Organic compounds were determined by thin-layer chromatography. The results for each plant were archived and analyzed for the presence of quercetin, mangiferin, luteolin, rutin, quercetin-2-D-glucoside, malvidin, as well as caffeic, cinnamic, ferulic, and sinapinic acids. Results and discussion. We developed a procedure for screening solvents and performed a fractional qualitative analysis of biologically active substances extracted from ginseng. As a result, we established the optimal parameters for extracting biologically active substances from the dried biomass of ginseng cultures. In all cases, temperature and the ratio of solvent to biomass were the same (50°C, 1:5). However, the extraction time and ethanol concentration differed, amounting to 60 min and 50% for callus cultures, 30 min and 60% for suspension cultures, and 60 min and 70% for root cultures. The qualitative analysis of organic compounds showed the presence of rutin (0.25), quercetin (0.75), and mangiferin (0.57), as well as caffeic and sinapinic acids in the extracts. Conclusion. Our set of experiments to isolate biologically active substances from ginseng callus, suspension, and root cultures resulted in selecting the optimal extraction parameters and analyzing the extracts for the presence of organic compounds.

Optimization of extraction parameters of biologically active substances from dried biomass of callus, suspension cells and root cultures in vitro

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/613/1/012008 ◽

2020 ◽

Vol 613 ◽

pp. 012008

Author(s):

L K Asyakina ◽

O O Babich ◽

A V Pungin ◽

A Yu Prosekov ◽

A D Popov ◽

...

Keyword(s):

Biologically Active ◽

Root Cultures ◽

Suspension Cells ◽

Extraction Parameters ◽

Determination of the Qualitative Composition of Biologically-Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhodiola rosea

Biomolecules ◽

10.3390/biom11030365 ◽

2021 ◽

Vol 11 (3) ◽

pp. 365 ◽

Cited By ~ 1

Author(s):

Lyudmila Asyakina ◽

Stanislav Sukhikh ◽

Svetlana Ivanova ◽

Alexander Prosekov ◽

Elena Ulrikh ◽

...

Keyword(s):

Medicinal Plant ◽

Cell Suspension ◽

Biologically Active ◽

Rhodiola Rosea ◽

Root Cultures ◽

Qualitative Composition ◽

Callus Cell ◽

The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures show that the main biologically active substances (BAS) in the medicinal plant, Rhodiola rosea, are 6-C-(1-(4-hydroxyphenyl)ethyl)aromadendrin (25 mg, yield 0.21%), 2-(3,7-dihydroxy-2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-yl)-6,7-dihydroxychroman-4-one (23 mg, yield 0.2%), 2-(3,4-dimethoxyphenyl)-5,7-dimethoxychroman-4-one (175 mg, yield 1.5%), 5,7-dihydroxy-2-(4-hydroxy-3-(2-(4-hydroxyphenyl)-4-oxo-4H-chromen-6-yl)phenyl)-4H-chromen-4-one (45 mg, yield 0.5%), 5,6,7,8-tetrahydroxy-4-methoxyflavone (0.35 mg, 0.5%). BAS from the dried biomass extracts of in vitro callus, cell suspension, and root cultures of Rhodiola rosea will be used for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial, and antioxidant effects.

Developmental Variations in the Content of Active Substances and Nutrients in Echinacea purpurea

HortScience ◽

10.21273/hortsci.33.3.518d ◽

1998 ◽

Vol 33 (3) ◽

pp. 518d-518

Author(s):

W. Letchamo ◽

V.S. Krutilina

Keyword(s):

Essential Oil ◽

Plant Development ◽

Maximum Yield ◽

Echinacea Purpurea ◽

The United States ◽

Biologically Active ◽

General Distribution ◽

Cichoric Acid ◽

Echinace from the Asteracea family has been among the top 10 marketed herbs in North America and Europe. So far, only a limited amount of Echinace originate from commercial cultivation. Echinacea purpurea has been commercially cultivated in the United States since the mid 1970s, in Russia since 1935, while in Europe since the 1960s. However, there has been no published information in the English language on methods of cultivation, expected yields, limiting factors, nutrient requirements, and the optimum plant development stage to obtain maximum yield and concentration of the biologically active substances for pharmaceutical and cosmetic processing. The objective of this investigation was, therefore, to study the general distribution of major nutrients and biologically active substances and the relationship between the two groups, and the pattern of the accumulation of cichoric acid, echnacoside, isobutylamides (tetraenes) and essential oil in commercially cultivated Echinacea purpurea. There was a significant variation in the content of essential oil, cichoric acid, and isobutylamides (tetraenes) in different organs and stages of the plant development. Similarly, the difference in the distribution of N, P, K, Ca, Mg, and Fe in different organs and developmental stages is demonstrated. The correlation between the content of the active substances and nutrients is demonstrated, while recommendations for an optimum harvesting stage and date are made.

Effect of some ions and biologically active substances on the acetylcholine-binding capacity of the blood in vitro

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00784811 ◽

1973 ◽

Vol 76 (1) ◽

pp. 809-811

Author(s):

R. A. Sokolinskaya

Keyword(s):

Binding Capacity ◽

Biologically Active ◽

Optimization of parameters of the extraction process of biologically active substances of grass Adonis vernalis

Technology audit and production reserves ◽

10.15587/2706-5448.2021.235471 ◽

2021 ◽

Vol 3 (3(59)) ◽

pp. 14-18

Author(s):

Anna Krvavych ◽

Roksolana Konechna

Keyword(s):

Phenolic Compounds ◽

Extraction Method ◽

Cardiac Glycosides ◽

Maximum Yield ◽

Particle Diameter ◽

Cardiac Activity ◽

Value Added ◽

Biologically Active ◽

The object of this research is the herb Adonis vernalis and hydroalcoholic extracts obtained on its basis. By the nature of the action, the drugs Adonis vernalis belong to the group of cardiac glycosides and occupy an intermediate place between strophanthin and digitalis. In official medicine, biologically active compounds of Adonis vernalis are included in drugs such as Cardiovalen, Adonis-bromine, Caridiolin and Cardiophyte. In the extemporal formulation, the extract is part of the Bechterew’s medicine. Until recently, scientists have made significant progress in studying the phytochemical and pharmacological action of the herb Adonis vernalis. However, one should not be limited to the use of Adonis vernalis only for stimulating cardiac activity, one should also consider the promising antioxidant effect of flavonoids and phenolic compounds that are contained in this medicinal plant. Also, studies of the extraction conditions of the herb Adonis vernalis are not described in the scientific literature. The study carried out the extraction of the grass Adonis vernalis by various methods (static and dynamic). The type of extract was chosen as the most rational, which provides the maximum yield of extractives (phenolic compounds and cardiac glycosides). For this, the optimal extraction conditions were selected, namely, the particle size, the type of extractant, the value of the hydromodule and the extraction method. Accordingly, the optimal particle diameter for maximum extraction of biologically active substances of the herb Adonis vernalis is 2.5 mm, the optimal extractant is 70 % ethyl alcohol, the ratio of raw materials: extractant is 1:10, the optimal extraction method is maceration with constant stirring. Phytochemical studies of the quantitative composition of the main groups of biologically active substances were carried out using calorimetric and spectrophotometric methods of analysis. As a result of the optimization of the process and its introduction into production on an industrial scale, the value added effect will be achieved. And also a high-quality product has been created that will compete with broad-spectrum drugs already existing on the market.

Biotechnological aspects of obtaining biologically active substances of Adonis vernalis

Chemistry, Technology and Application of Substances ◽

10.23939/ctas2021.01.126 ◽

2021 ◽

Vol 4 (1) ◽

pp. 126-130

Author(s):

V. R. Hamada ◽

Keyword(s):

Antioxidant Activity ◽

Biological Activity ◽

Raw Materials ◽

Callus Cultures ◽

Biologically Active ◽

In Vitro Cultivation ◽

Plant Raw Materials ◽

This article shows the possibility of using a biotechnological method to obtain biologically active compounds based on in vitro cultivation of callus cultures. The callus biomass of Adonis vernalis was obtained in vitro by using the biotechnological method. The extracts based on callus biomass of Adonis vernalis were obtained. The content of flavonoids and phenolic compounds was determined. The antioxidant activity of callus biomass of Adonis vernalis has also been studied. The research results show that the content of biologically active substances and biological activity in callus biomass does not differ from plant raw materials.

Optimization of biotechnological processes in the production of apple juice

BIO Web of Conferences ◽

10.1051/bioconf/20213406003 ◽

2021 ◽

Vol 34 ◽

pp. 06003

Author(s):

Natalia Droficheva

Keyword(s):

Technological Process ◽

Enzyme Preparation ◽

Apple Juice ◽

Raw Materials ◽

Biologically Active ◽

Optimal Parameters ◽

Enzyme Preparations ◽

Biotechnological Processes ◽

When obtaining apple juice, the issue of optimizing biotechnological processes is acute. The use of enzyme preparations of different specifics of the action allows you to choose the optimal parameters of the technological process and select the drug taking into account the purpose ofuse. The influence of enzyme preparations: Trinoline 4000, Trinolin DF, Fructocyme P6-L, Lafaze on the yield of juice and biologically active substances in the processing of fruit raw materials was studied. An enzyme preparation that maximizes the yield of apple juice has been isolated. Their effect on the extraction of biologically active substances has been determined.