scholarly journals Cytotoxic Effect of Capsicum annum L. extract on T47D Cells: In vitro Study

2021 ◽  
pp. 3389-3392
Author(s):  
Rochmah Kurnijasanti ◽  
Amaq Fadholly
Keyword(s):  
Cytotoxic Effect ◽  
Caspase 3 ◽  
Anticancer Agent ◽  
In Vitro Study ◽  
Pharmacological Effects ◽  
Apoptosis Induction ◽  
T47d Cells ◽  
Capsicum Annum ◽  
Ic50 Value

Capsicum annum L. is a potential natural plant that have a lot of various pharmacological effects, including as anticancer agent. This study Aim to analyze Capsicum annum extract (CAE) on T47D cells. CAE (10, 20, 40, 60, 80µg/mL) treated on T47D cells to determined IC50 value by MTT assay. Apoptosis induction is also investigated through caspase-3 expressions (IC50, 2IC50). The present study showed that CAE suppress T47D cells proliferation with IC50 value of 75.81µg/mL. The caspase-3 expression on 2IC50 is higher (67.16%) than IC50 (52.16%). This result indicate that CAE has ability as anticancer agent by inhibiting cell growth and induce apoptosis through caspase-3 expression on T47D cells. Further study of CAE holds potential for novel therapies of cancer prevention and treatment.

scholarly journals Effects of Silver Nanoparticles on Proliferation and Apoptosis in Granulosa Cells of Chicken Preovulatory Follicles: An In Vitro Study

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1652
Author(s):  
Dorota Katarzyńska-Banasik ◽  
Anna Kozubek ◽  
Małgorzata Grzesiak ◽  
Andrzej Sechman
Keyword(s):  
Silver Nanoparticles ◽  
Granulosa Cells ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Poultry Production ◽  
Cell Death Pathways ◽  
Preovulatory Follicles ◽  
Proliferation And Apoptosis ◽  
Apoptosis Process

The continuous development of poultry production related to the growing demand for eggs and chicken meat makes it necessary to use modern technologies. An answer to this demand may be the use of nanotechnology in poultry farming. One of the promising nanomaterials in this field are silver nanoparticles (AgNPs), which are used as disinfectants, reducing microbial pollution and the amounts of greenhouse gases released. This study aimed to evaluate the effect of AgNPs on the proliferation and apoptosis process in the granulosa cells of chicken preovulatory follicles. The in vitro culture experiment revealed that both 13 nm and 50 nm AgNPs inhibited the proliferation of the granulosa cells. However, a faster action was observed in 50 nm AgNPs than in 13 nm ones. A size-dependent effect of AgNP was also demonstrated for the caspase-3 activity. AgNPs 13 nm in size increased the caspase-3 activity in granulosa cells, while 50 nm AgNPs did not exert an effect, which may indicate the induction of distinct cell death pathways by AgNPs. In conclusion, our study reveals that AgNPs in vitro inhibit granulosa cell proliferation and stimulate their apoptosis. These results suggest that AgNPs may disrupt the final stage of preovulatory follicle maturation and ovulation.

scholarly journals Investigation of characterization and cytotoxic effect of PEGylated nanoliposomal containing Melphalan on ovarian cancer: an in vitro study

2021 ◽  
Vol 16 (1) ◽  
pp. 102-116
Author(s):  
Seyedeh Masoomeh Sadat Mirnezami ◽  
Amir Heidarinasab ◽  
Azim Akbarzadeh ◽  
Mehdi Arjmand
Keyword(s):  
Ovarian Cancer ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Vitro Study

Diallyl Disulfide Attenuates Methotrexate-Induced Hepatic Oxidative Injury, Inflammation and Apoptosis and Enhances its Anti-tumor Activity

2021 ◽  
Vol 14 ◽  
Author(s):  
Marwa M. Khalaf ◽  
Emad H.M. Hassanein ◽  
Abdel-Gawad S. Shalkami ◽  
Ramadan A.M. Hemeida ◽  
Wafaa R. Mohamed
Keyword(s):  
Cytotoxic Activity ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Diallyl Disulfide ◽  
Sod Activity ◽  
Cytotoxic Effects ◽  
Wide Range ◽  
Anti Tumor Activity ◽  
Mcf 7

Background: Methotrexate (MTX) is used potently for a wide range of diseases. However, hepatic intoxication by MTX hinders its clinical use. Objectives: The present study was conducted to investigate the diallyl disulfide (DADS) ability to ameliorate MTX-induced hepatotoxicity. Methods: Thirty-two rats were randomly divided into four groups: normal control, DADS (50 mg/kg/day, orally), MTX (single i.p. injection of 20 mg/kg) and DADS+MTX. Liver function biomarkers, histopathological examinations, oxidative stress, inflammation, and apoptosis biomarkers were investigated. Besides, an in vitro cytotoxic activity study was conducted to explore the modulatory effects of DADS on MTX cytotoxic activity using Caco-2, MCF-7, and HepG2 cells. Results: DADS significantly reduced the increased serum activities of ALT, AST, ALP, and LDH. These results were confirmed by the alleviation of liver histopathological changes. It restored the decreased GSH content and SOD activity, while significantly decreased MTX-induced elevations in both MDA and NO2- contents. The hepatoprotective effects were mechanistically mediated through the up-regulation of hepatic Nrf-2 and the down-regulation of Keap-1, P38MAPK, and NF-κB expression levels. In addition, an increase in Bcl-2 level with a decrease in the expression of both Bax and caspase-3 was observed. The in vitro study showed that DADS increased MTX anti-tumor efficacy. Conclusions: DADS potently alleviated MTX-induced hepatotoxicity through the modulation of Keap-1/Nrf-2, P38MAPK/NF-κB and apoptosis signaling pathways and effectively enhanced the MTX cytotoxic effects, which could be promising for further clinical trials.

The Cytotoxic Effect of Combined Hyperthermia and Taxane Chemotherapy on Ovarian Cancer Cells: Results of an in vitro Study

2012 ◽  
Vol 48 (2) ◽  
pp. 55-63 ◽  
Author(s):  
M. Muller ◽  
M. Chérel ◽  
P.-F. Dupré ◽  
S. Gouard ◽  
M. Collet ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ovarian Cancer Cells ◽  
Taxane Chemotherapy

Cytotoxic Effect of Allium cepa L. Extract on Human colon Cancer (WiDr) Cells: In Vitro Study

2019 ◽  
Vol 12 (7) ◽  
pp. 3483
Author(s):  
Amaq Fadholly ◽  
Arif N. M. Ansori ◽  
Shara Jayanti ◽  
Annise Proboningrat ◽  
Muhammad K. J. Kusala ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Allium Cepa ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Human Colon ◽  
Vitro Study ◽  
Human Colon Cancer ◽  
Allium Cepa L

scholarly journals Effects of griseofulvin on apoptosis through caspase-3- and caspase-9-dependent pathways in K562 leukemia cells: An in vitro study

2010 ◽  
Vol 71 (6) ◽  
pp. 384-397 ◽  
Author(s):  
Ning Zhong ◽  
Hankui Chen ◽  
Quanlin Zhao ◽  
Hongwei Wang ◽  
Xin Yu ◽  
...  
Keyword(s):  
Caspase 3 ◽  
In Vitro Study ◽  
Vitro Study ◽  
Leukemia Cells ◽  
Caspase 9

Lipid emulsion attenuates apoptosis induced by a toxic dose of bupivacaine in H9c2 rat cardiomyoblast cells

2016 ◽  
Vol 35 (9) ◽  
pp. 929-937 ◽  
Author(s):  
S-H Ok ◽  
J Yu ◽  
Y Lee ◽  
H Cho ◽  
I-W Shin ◽  
...  
Keyword(s):  
Cell Viability ◽  
Lipid Emulsion ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Caspase 8 ◽  
Lipid Solubility ◽  
H9c2 Cells ◽  
Toxic Dose

The goal of this in vitro study was to investigate the effect of lipid emulsion on apoptosis induced by a toxic dose of bupivacaine (BPV) in H9c2 rat cardiomyoblast cell lines. The effect of lipid emulsion on the decreased cell viability and count induced by BPV or mepivacaine (MPV) in the H9c2 cells was assessed using an 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay or a cell count assay. The effect of BPV or lipid emulsion combined with BPV on cleaved caspase 3, caspase 8, and Bax in H9c2 cells was investigated using Western blotting. A terminal deoxynucleotidyl transferase dUTP2′-deoxyuridine 5′-triphosphate nick end-labeling (TUNEL) assay was performed to detect apoptosis of H9c2 cells treated with BPV alone or lipid emulsion combined with BPV. The magnitude of lipid emulsion-mediated attenuation of decreased cell viability induced by BPV was higher than that of lipid emulsion-mediated attenuation of decreased cell viability induced by MPV. Lipid emulsion attenuated the increases in cleaved caspase 3, caspase 8 and Bax induced by BPV. Lipid emulsion attenuated the increases in TUNEL-positive cells induced by BPV. These results suggest that lipid emulsion attenuates a toxic dose of BPV-induced apoptosis via inhibition of the extrinsic and intrinsic apoptotic pathways. The protective effect of lipid emulsion may be partially associated with the relatively high lipid solubility of BPV.

Cytotoxic effect of neuromyelitis optica antibody (NMO-IgG) to astrocytes: An in vitro study

2009 ◽  
Vol 215 (1-2) ◽  
pp. 31-35 ◽  
Author(s):  
Lidia Sabater ◽  
Albert Giralt ◽  
Anna Boronat ◽  
Karolina Hankiewicz ◽  
Yolanda Blanco ◽  
...  
Keyword(s):  
Neuromyelitis Optica ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Antimicrobial Activity and Cytotoxicity of Mouthwash Prepared from Azadirachta indica and Stevia rebaudiana Extract– An In vitro Study

2021 ◽  
pp. 96-107
Author(s):  
Bipin Maheshwaran ◽  
R. Priyadharshini ◽  
S. Rajesh Kumar ◽  
Palati Sinduja
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Plant Extract ◽  
Azadirachta Indica ◽  
Cytotoxic Effect ◽  
Stevia Rebaudiana ◽  
In Vitro Study ◽  
Plaque Development ◽  
Mouth Wash

Introduction: Mouth wash are generally utilized as subordinates to oral cleanliness and in the conveyance of dynamic specialists to the teeth and gums. These flushes can impact plaque development and adjust the course of gingival irritation. Azadirachta indica (Neem) was utilized to treat different skin illnesses, as a disinfectant substance and as a natural mouthwash. Stevia rebaudiana can inhibit the growth of microorganisms that are responsible for dental caries. Aim: This study aimed to assess the antimicrobial activity and cytotoxicity from Neem and Stevia based Mouthwash. Materials and Methods: Plant extract was prepared and an antimicrobial and cytotoxic effect was done by considering various parameters. The antimicrobial activity of nanoparticles prepared using plant extract was investigated and the results of the test were described as the standard deviation and analyzed. For the cytotoxic activity, an ELISA plate was used, wherein the mortality rate of the nauplii was estimated with the plant extract mediated nanoparticles at different concentrations. Data’s were statistically analyzed by Spearman correlation through SPSS version 23. Results: Anti-microbial activity showed positive correlation with increase in concentration (r=1). The cytotoxic activity showed negative correlation with the number of live nauplii decreased in the second day when compared to the first day suggesting that the extract has potent cytotoxic activity (r=-1). Conclusion: Neem and Stevia extract helped us to detect the antimicrobial activity and cytotoxic effect on the various species in different concentration levels. The study needs to be evaluated further for isolating the possible compounds to test the effectiveness of antimicrobial activity in the oral cavity of the human body to prevent various diseases.

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