Optimization of phenolics and antioxidants extraction from Centaurium erythraea using response surface methodology

2021 ◽  
pp. 6455-6462
Author(s):  
Wissam Zam ◽  
Ali Ali ◽  
Dimah Saleem ◽  
Sahar Alali
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Volume Fraction ◽  
Quadratic Model ◽  
Natural Food ◽  
Solvent Ratio ◽  
Research Attention ◽  
Experimental Values ◽  
Centaurium Erythraea

In recent years, Centaurium erythraea extracts have attracted much research attention in the context of prevention or treatment of many diseases due to its bioactive compounds content and antioxidant activity. The antioxidants of C. erythraea are very effective as they possess excellent antioxidant activity. Thus, it can be used as a safe and natural food preservative. The aim of this study is to make extracts more effective by optimizing the extraction conditions of the phenolics and antioxidants from C. erythraea using response surface methodology (RSM) based on a central composite design (CCD). Two process variables (Methanol volume fraction and solid - solvent ratio) were evaluated at five levels (13 experimental designs). Multiple regression analyses were performed to obtain quadratic polynomial equations using RSM; each response was fitted by a quadratic model. The adequacy of the models was proven using the analysis of variance (ANOVA). The significant effects of the factors and their interactions on the extraction efficiency were investigated at 95% confidence interval. RSM indicated that the optimal extraction conditions were 71% methanol volume fraction and 2.2:10 solid:solvent ratio. Predicted values thus obtained were close to the experimental values indicating suitability of the model.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

2016 ◽  
Vol 34 (No. 4) ◽  
pp. 341-349 ◽  
Author(s):  
Q. He ◽  
Y. Li ◽  
P. Zhang ◽  
A. Zhang ◽  
H. Wu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Apium Graveolens ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Inhibition Rate ◽  
Microwave Assisted ◽  
Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

scholarly journals Effects of Time, Temperature, and Solvent Ratio on the Extraction of Non-Extractable Polyphenols with Anticancer Activity of Barhi Date Palm Kernels Extracts Using Response Surface Methodology

2019 ◽  
Author(s):  
Israa Mahmud ◽  
Mohamed E. S. Mirghani ◽  
Faridah Yusof ◽  
Ma'an Al-khatib
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Date Palm ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Dietary Polyphenols ◽  
Experimental Values

Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro.

scholarly journals Effect of nutrient sources on bench scale vinegar production using response surface methodology

2005 ◽  
Vol 9 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Joelma M. Ferreira ◽  
Ramdayal Swarnakar ◽  
Flávio L. H. da Silva
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Nitrogen Sources ◽  
Quadratic Model ◽  
Small Scale ◽  
Nutrient Sources ◽  
Bench Scale ◽  
Model Equations ◽  
Experimental Values ◽  
Star Configuration

The present work aims to evaluate on a bench scale, the effects of nitrogen and phosphorous nutrient source concentrations in vinegar production, a process that is used by small scale industries in the State of Paraiba. The response surface methodology has been utilized for modeling and optimization of the fermentation process. Initially a 2³ complete factorial design was used, where the effects of initial concentrations of ethyl alcohol, phosphorous and nitrogen sources were observed. After this analysis the concentration range of the nutrient variables were extended and a two level plus a star configuration factorial experimental design was performed. The experimental values are well represented by the linear and quadratic model equations obtained. The optimum concentration of ethanol was 4% in which the yield and the productivity of the acetic acid were maximized to the values of 70% and 0.87 g L-1 h-1 respectively, for a 24 hours fermentation period. The evaluation of the quadratic models showed that the yield of vinegar is maximized from 28.1 to 51.04% and the productivity from 0.69 to 1.29 g L-1 h-1 when the concentration of the nitrogen nutrient in the medium is increased from 0.2 to 2.3 g mL-1. Thus, at the optimized nitrogen nutrient concentration both the yield and the productivity of the vinegar are increased by 1.85 times.

Performance Optimization of Trapezium Rib Parameters Using Response Surface Methodology

2017 ◽  
Author(s):  
Naveen Sharma ◽  
Vaibhav Sharma ◽  
Andallib Tariq
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Hydraulic Diameter ◽  
Quadratic Model ◽  
Design Parameters ◽  
Geometrical Parameters ◽  
Performance Parameters ◽  
Rectangular Duct ◽  
Height Ratio ◽  
Experimental Values

In the present work, transient liquid crystal thermography (LCT) has been used for capturing the temperature field as well as the local heat transfer distribution inside a rectangular duct. Experiments have been carried out in an open loop airflow system at a Reynolds number (based on the channel hydraulic diameter) of 58850 and for rib height to channel hydraulic diameter ratio of 0.125. This investigation emphases headed for assessing the potential impact of design parameters such as chamfering angle and rib pitch to height ratio of the trapezium ribbed rectangular duct on the thermo-hydraulic performances, which forms the basis of analysis while using response surface methodology (RSM). The chamfering angle has been varied from 0 to 20° in a step of 5°, while the rib pitch to height ratio is varied from 8 to 12 in a step of 2. The quadratic model generated by RSM is used to predict the optimal performance parameters. The results show that different geometrical parameters have to be considered simultaneously in order to improve the performance of ribbed-duct. Eventually, based on this analysis, the optimum levels of design parameters for trapezium rib corresponding to the highest augmentation Nusselt number, the lowest friction factor, and the highest thermo-hydraulic performance have been determined. Finally, the desired correlations for all performance parameters have been developed using RSM. The comparison of predicted values with the experimental values has been carried out, which is found to be in harmony with the experimental values in the uncertainty range of ±5%., which are found to predict the performance parameters with reasonably good accuracy.

scholarly journals Optimization of reflux extraction for cat’s whiskers leaves extract using response surface methodology

2020 ◽  
Vol 26 (1) ◽  
pp. 49-57
Author(s):  
Mohd Ariff ◽  
Norfazilah Abdullah
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Solvent Ratio ◽  
Operation Conditions ◽  
Central Composite Experimental Design ◽  
Cat’S Whiskers

Cat?s whiskers (Orthosiphon stamineus) is a medicinal plant which comprises several dynamic pharmacological properties such as anti-inflammatory, antioxidant and antibacterial. In small and medium-scale industries, conventional reflux extraction method is favored as compared to other non-conventional extraction methods due to cost effectiveness and simple operating procedures. In this study, response surface methodology (RSM) was applied to optimize the reflux conditions for extraction of cat?s whiskers leaves in order to achieve a high content of antioxidant activity in the extracts. Central composite experimental design (CCD) with three factors and three levels was employed to consider the effects of the operation conditions. Antioxidant activity of the extracts were based on free radical scavenging activity (DPPH assay) and were analyzed using a UV-Vis spectrophotometer. Based on RSM, the antioxidant activity could be maximized when the operation conditions were 125 ?m for particle size, 1.5:20 for sample-to-solvent ratio, and 2 h for extraction time. Under these optimal conditions, the predicted value of the antioxidant activity was compared with the actual, and the mean error was 0.46%. This indicates the suitability of the model for optimizing the conditions for the reflux extraction of cat?s whiskers leaves.

scholarly journals Optimization of subcritical water extraction of phenolic compounds from Ziziphus jujuba using response surface methodology: evaluation of thermal stability and antioxidant activity

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Razieh Niazmand ◽  
Mostafa Shahidi Noghabi ◽  
Azam Niazmand
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Subcritical Water ◽  
Solvent Ratio ◽  
Bulk Oil ◽  
Emulsion Model ◽  
Ziziphus Jujuba

Abstract Background The jujube is mainly grown in the subtropical and tropical regions of Asia. Due to owning bioactive compounds such as polyphenols, it was considered as medicinal and nutritional plant in traditional medicine. This study aimed to extract phenolic compounds from Ziziphus jujuba using subcritical water (SCW) process. The possible combinations of temperature, time, and fruit-to-solvent ratio were investigated using response surface methodology. Results The total phenolic compounds (TPC) and radical scavenging capacity (RSC) of 975.94 mg/g and 53.98%, respectively, were recovered at optimum extraction conditions (170 °C, 74.49 min, and fruit-to-solvent ratio of 1:5.29). The extract obtained in SCW optimum conditions was put under thermal treatments including low temperature long time, high temperature short time, cooking, baking, and sterilization. The results showed after baking, the amount of TPC, RSC, and absorbance at 420 nm increased. The quantity of gallic acid, chlorogenic acid, p-coumaric acid, ferulic acid, and rutin increased significantly. The efficiency of SCW extract in retarding lipid oxidation in bulk oil and O/W emulsion model was investigated at 50 °C for 10 days. After 10 days, the peroxide value of bulk oil (1.1 meq O2/kg) containing SCW extract was lower than O/W emulsion model (2.2 meq O2/kg). Conclusion The subcritical extracted phenolic compounds seem to have good antioxidant activity as well as thermal stability for using in food or drug industries.

Optimization of Phenolic Compound Recovery and Antioxidant Activity of Bay Leaves Using Sequential and Response Surface Methodologies

2021 ◽  
Vol 17 ◽  
Author(s):  
Yazid Khaled Khodja ◽  
Mostapha Bachir-Bey ◽  
Rachid Ladjouzi ◽  
Bachra Khettal
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Laurus Nobilis ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Experimental Values

Background: The laurel is a shrub native to the Mediterranean basin. It is too often wrongly relegated to the kitchen as a simple condiment while it possesses many therapeutic properties and virtues. The valorization of this plant in terms of bioactive compounds seems to be important. Objective: The aim of the present study is to optimize total phenolic content (TPC) extraction and antioxidant activity from dried bay leaves using conventional process and response surface methodology (RSM). Method: The study of optimization was firstly tested three extraction methods (maceration, ultrasonic-assisted extraction (UAE), and Microwave-assisted extraction (MAE)) then the best one was considered for RSM. Results : The results for the sequential procedure indicated that MAE was the best method for TPC extraction and antioxidant activity of Laurus nobilis leaves. The mathematical modeling of MEA using response surface methodology showed that optimal conditions for phenolic compounds extraction were 57% methanol with microwaves power of 602W during 3.48 minutes. These conditions allowed TPC extraction of 83.53 mg/g and manifested an antioxidant activity of 32.51 mg EAG/g. Conclusion: The validation of models indicated that experimental values were in accordance with predicted ones, demonstrating the suitability of developed models and the success of RSM in the optimization of antioxidants extraction from Laurus nobilis leaves. Therefore, the bay leaves could be considered as an interesting source of phenolic antioxidants.

Application of Response Surface Methodology and Central Composite Rotatable Design for Modeling and Optimization of Catalyst Compositions in Ethanol Synthesis via CO Hydrogenation

2014 ◽  
Vol 12 (1) ◽  
pp. 245-255
Author(s):  
Fang Li ◽  
Hongfang Ma ◽  
Weiyong Ying
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Co Hydrogenation ◽  
Quadratic Model ◽  
Central Composite Rotatable Design ◽  
Co Conversion ◽  
Experimental Values ◽  
Central Composite ◽  
Ethanol Synthesis ◽  
Rotatable Design

Abstract A statistical analysis about the effect of catalyst compositions on ethanol synthesis from CO hydrogenation was studied. The effect of Rh loading (0–3 wt.%), Fe loading (2–10 wt.%) and Mn loading (0.5–2.5 wt.%) of RhMnFe/γ-Al2O3 was studied through response surface methodology (RSM) combined with a central composite rotatable design (CCRD). A linear and a quadratic model were proposed to correlate the three variables to the two responses: CO conversion and ethanol selectivity. The predicted values for ethanol selectivity were in a good agreement with the experimental values, with R2 of 0.9779. The optimum conditions for achieving the maximum ethanol selectivity (27.8%) while limiting CO conversion at a moderate level (>20%) were as follows: Rh loading of 2.5 wt.%, Mn loading of 2.5 wt.% and Fe loading of 4 wt.%. Two representing catalysts were characterized by XRD, TPR and DRIFTS.

Optimisation of the Oil Extraction from Nigella sativa Seeds Using Response Surface Methodology

2017 ◽  
Vol 68 (2) ◽  
pp. 331-336
Author(s):  
Gabriela Isopencu ◽  
Mirela Marfa ◽  
Iuliana Jipa ◽  
Marta Stroescu ◽  
Anicuta Stoica Guzun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Nigella Sativa ◽  
Polynomial Equation ◽  
Herbaceous Plant ◽  
Box Behnken Design ◽  
Pareto Analysis ◽  
Black Cumin ◽  
Mediterranean Countries ◽  
Experimental Values

Nigella sativa, also known as black cumin, an annual herbaceous plant growing especially in Mediterranean countries, has recently gained considerable interest not only for its use as spice and condiment but also for its healthy properties of the fixed and essential oil and its potential as a biofuel. Nigella sativa seeds fixed oil, due to its high content in linoleic acid followed by oleic and palmitic acid, could be beneficial to human health. The objective of this study is to determine the optimum conditions for the solvent extraction of Nigella sativa seeds fixed oil using a three-level, three-factor Box-Behnken design (BBD) under response surface methodology (RSM). The obtained experimental data, fitted by a second-order polynomial equation were analysed by Pareto analysis of variance (ANOVA). From a total of 10 coefficients of the statistical model only 5 are important. The obtained experimental values agreed with the predicted ones.

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