scholarly journals GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

2018 ◽  
Vol 5 (4) ◽  
pp. 79 ◽  
Author(s):  
Pilar A. Soledispa ◽  
José González ◽  
Armando Cuéllar ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Mass Spectra ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
Main Components ◽  
First Time

A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of Smilax domingensis Wid. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

scholarly journals Characterization by GC-MS of the Main Ethanolic Extract’S Components from the Bark of Talipariti elatum Sw. (Fryxell) Malvaceae

2018 ◽  
Vol 6 (2) ◽  
pp. 1
Author(s):  
José González ◽  
Armando Cuéllar ◽  
Enrique Gómez ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Quality Control ◽  
Crude Extract ◽  
Mass Spectra ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
First Time

Talipariti elatum (Sw.), commonly named Blue Mahoe, is used as antasthmatic, antitussive, against catarrh and expectorant in phytotherapy in Cuba, although the plants’ composition has not been determined in detail so far. An ethanolic extract from the bark of Talipariti elatum Sw. (Fryxell) Malvaceae that grows in Cuba was prepared by Soxhlet extraction with ethanol at 95% during 20 hours. The crude extract without purification was analyzed using a GCMS-QP2010 Ultra Shimadzu, and the components were identified by comparing their retention times and their mass spectra with those of NIST21Library. About 45 different chemical compounds were detected in the sample and from them, 31 chemical components were characterized and reported for the first time from Talipariti elatum. Our results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Talipariti samples.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

scholarly journals LC-ESI-HRMS-Based Chemical Characterization of Lupinus bogotensis Roots

2016 ◽  
Vol 12 (2) ◽  
pp. 200-211
Author(s):  
Lorena Vargas-Medina ◽  
Lydia F. Yamaguchi ◽  
Ericsson Coy-Barrera
Keyword(s):  
Chemical Composition ◽  
Native Species ◽  
Mass Spectra ◽  
Chemical Nature ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Industrial Applications ◽  
Tentative Identification ◽  
Resolution Mass

Plants of the genus Lupinus (Fabaceae) have been studied due to the occurrence of different compounds, especially those possessing quinolizidine and isoflavone-like structures. These kinds of compounds are characterized by both medical and industrial applications, providing various benefits to human being. However, organs such as roots have not been equally studied and there is a lack of such records for native species. Therefore, in the present study, the chemical composition of nodulated roots from greenhouse-established L. bogotensis plants was determined. The resulting crude ethanolic extract was then analyzed by LC/HRMS and chemical nature of most compounds was determined by analyzing the high resolution mass spectra. Recorded profile showed adequate separation allowing tentative identification of detected compounds. 47 secondary metabolites (mainly isoflavones and quinolizidine-type compounds) were thus identified. Most phenolic compounds were found to be conjugated flavonoids (e.g., genistin and genistein malonylglucoside) and lupanine, sparteine and hydroxylupanine were noticed as the main alkaloids. Among alkaloid-like compounds, dehydromitomycin C, a compound produced by Streptomyces caespitosus was identified. Lupadienediol (a lupane-type triterpene recognized for being involved in rhizobacteria:legumes symbiosis) was the only terpene-related component identified in the extract. The present work corresponds to the first report on the chemical composition of L. bogotensis root and constitutes an adequate basis for phytoconstituents finding from nature to support the use of native species.

scholarly journals Chemical Characterization of Metabolites from the Husk of Theobroma cacao by GC-MS in Cuba

2018 ◽  
Vol 6 (2) ◽  
pp. 39
Author(s):  
Danae Perez ◽  
José González ◽  
Enrique Gómez ◽  
Julio Perez ◽  
José L. Rodríguez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Phenolic Compounds ◽  
Theobroma Cacao ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Phytochemical Screening

n ethanolic extract at 90% made by Soxhlet was analyzed using a Shimadzu spectrometer coupled to gas chromatography (GCMS-QP2010 Ultra) after derivatization with BSTFA from the husk of Theobroma cacao. The drug powdered was evaluated through a phytochemical screening to get information about the chemical components present in the sample. Ethereal, ethanolic and aqueous extract were analyzed by the same GCMS technique. Gas Chromatography Mass Spectrometry (GC-MS) analysis revealed the presence of 74 metabolites from this husk. According to NIST21 and NIST107 Libraries Databases some of the phyto-compounds screened were Caffeine, Theobromine, Catechine, Uridine, Stigmasterol, Cholesterol and Androstane-11,17-dione,3-[(trimethylsilyl)oxy]-,17-[O-(phenyl methyl)oxime]. Phytochemical screening revealed the possible presence of fats, alkaloids, triterpenes and steroids, catechins, reductants sugars, saponins, phenolic compounds, aminoacids or amines, quinones, anthocyanidins, flavonoids and bitter or astringent principles.

scholarly journals Extraction, Isolation and Characterization of Phytoconstituents in Urginea wightii

2020 ◽  
pp. 1-11
Author(s):  
M. Smitha Raj ◽  
M. N. Shiva Kameshwari
Keyword(s):  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Analytical Techniques ◽  
Molecular Structures ◽  
Chemical Components ◽  
Analytical Technique ◽  
Isolation And Characterization ◽  
Screening Study ◽  
Interesting Compound

The Urginea wightii is a well-established squill. The therapeutic activity is determined by the presence of pharmacological component present in the crude extract of Urginea wightii. To screen these active herbal components in a single plant material it requires a sophisticated, sensitive and a reliable analytical technique. As a preliminary screening study infrared spectrum provided information related to active functional groups of bulbous extract. This encouraging result evoked us for further accurate detailing of chemical components by advanced analytical techniques like liquid chromatography mass spectroscopy (LC-MS) and Nuclear magnetic resonance (NMR). The identified chemical constituents with possible molecular structures of pharmacological activity by LC-MS was found to be stigmasterol, hexadecanoic acid methyl ester and 1,3,7,11,15-tetramethyl-2- hexadecenol. Similarly, when 1H NMR was performed an interesting molecule (2,3-dihydro-dihydroxy-6-methyl-4H-pyran-4-one) having potential capability in destroying free radicals (antioxidant) was obtained. The comprehensive and qualitative characterization of these bioactive compounds present in Urginea wightii can be a useful in treating vast disease conditions. This investigation paves the way to explore researchers for further use of Urginea wightii as sources of medicinally interesting compound.

Chemical Constituents and Antibacterial Activity of Cissus Aralioides.

2020 ◽  
Vol 17 ◽  
Author(s):  
Balogun Olaoye Solomon ◽  
Ajayi Olukayode Solomon ◽  
Owolabi Temitayo Abidemi ◽  
Oladimeji Abdulkarbir Oladele ◽  
Liu Zhiqiang
Keyword(s):  
Plant Extract ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Sub Saharan Africa ◽  
Chromatographic Purification ◽  
Whole Plant ◽  
Sub Saharan ◽  
First Time

: Cissus aralioides is a medicinal plant used in sub-Saharan Africa for treatment of infectious diseases; however the chemical constituents of the plant have not been investigated. Thus, in this study, attempt was made at identifying predominant phytochemical constituents of the plant through chromatographic purification and silylation of the plant extract, and subsequent characterization using spectroscopic and GC-MS techniques. The minimum inhibitory concentration (MICs) for the antibacterial activities of the plant extract, chromatographic fractions and isolated compounds were also examined. Chromatographic purification of the ethyl acetate fraction from the whole plant afforded three compounds: β-sitosterol (1), stigmasterol (2) and friedelin (3). The phytosterols (1 and 2) were obtained together as a mixture. The GC-MS analysis of silylated extract indicated alcohols, fatty acids and sugars as predominant classes, with composition of 24.62, 36.90 and 26.52% respectively. Results of MICs indicated that friedelin and other chromatographic fractions had values (0.0626-1.0 mg/mL) comparable with the standard antibiotics used. Characterization of natural products from C. aralioides is being reported for the first time in this study.

scholarly journals Characterization of calcium oxide in root perforation sealer materials

2012 ◽  
Vol 23 (5) ◽  
pp. 539-546 ◽  
Author(s):  
Carlos Estrela ◽  
Manoel Damião Sousa-Neto ◽  
Orlando Aguirre Guedes ◽  
Ana Helena Gonçalves Alencar ◽  
Marco Antonio Hungaro Duarte ◽  
...  
Keyword(s):  
Calcium Oxide ◽  
Mineral Trioxide Aggregate ◽  
Chemical Compounds ◽  
Internal Diameter ◽  
X Ray ◽  
Final Sample ◽  
Materials Used ◽  
Main Components ◽  
White Mineral

Root perforation represents an undesirable complication that may lead to an unfavorable prognosis. The aims of this study were to characterize and to compare the presence of calcium oxide (CaO) on the chemical composition of materials used for root perforation therapy: gray and white mineral trioxide aggregate (MTA) and Portland cement (PC), gray MTA+5%CaO and gray MTA+10%CaO. The last two materials were analyzed to evaluate the increase of CaO in the final sample. CaO alone was used as a standard. Eighteen polyethylene tubes with an internal diameter of 3 mm and 3 mm in length were prepared, filled and then transferred to a chamber with 95% relative humidity and a temperature of 37ºC. The chemical compounds (particularly CaO) and the main components were analyzed by energy-dispersive X-ray microanalysis (EDX). EDX revealed the following concentrations of CaO: gray MTA: 59.28%, white MTA: 63.09%; PC: 72.51%; gray MTA+5%CaO: 63.48% and gray MTA+10%CaO: 67.55%. The tested materials presented different concentrations of CaO. Even with an increase of 5 and 10% CaO in gray MTA, the CaO levels found in the MTA samples were lower than those found in PC.

Determination of Bioactive Components of 60°С Volatiles from Cinnamomum camphora Branches by TD-GC/MS

2011 ◽  
Vol 230-232 ◽  
pp. 852-856
Author(s):  
Qing Li ◽  
Dang Quan Zhang ◽  
Qi Mei Liu ◽  
Kuan Peng
Keyword(s):  
Chemical Compounds ◽  
Chemical Components ◽  
Cinnamomum Camphora ◽  
Bioactive Components ◽  
Industrial Materials ◽  
Analytical Result ◽  
Main Components

The chemical components of helium volatiles from the fresh branches of Cinnamomum camphora were studied by TD-GC/MS. The analytical result by 60°С-based TD-GC/MS showed that 55 peaks were obtained from the helium volatiles from the fresh branches of Cinnamomum camphora and 53 chemical compounds were identified. The results showed that the main components were as: Bicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-, (1R)- (15.4328%), 1,3-Benzodioxole, 5-(2-propenyl)- (14.881%), Tricyclo[2.2.1.0(2,6)]heptane, 1,7-dimethyl-7-(4-methyl-3-pentenyl)-, (-)- (12.694%), p-menth-1-en-8-ol (9.832%), Bicyclo[2.2.1]heptane, 2-methyl-3-methylene-2-(4-methyl-3- pentenyl)-, (1S-exo)- (6.143%), 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl- (5.365%), Bicyclo[3.1.1] hept-2-ene, 2,6-dimethyl-6-(4-methyl-3-pentenyl)- (4.527%), Naphthalene, 1,2,3,5,6,8a- hexahydro-4,7-dimethyl-1-(1-methylethyl)-, (1S-cis)- (4.129%), 3-Cyclohexen-1-ol, 4-methyl-1- (1-methylethyl)- (2.965%), Borneol (2.627%), Bicyclo[2.2.1]heptan-2-ol, 1,7,7-trimethyl-, acetate, (1S-endo)- (2.586%), Copaene (2.534%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (1.612%), (-)-Isosativene (1.121%), etc. The analytical result suggested that the helium volatiles from the fresh branches of Cinnamomum camphora could be used as industrial materials of biomedicines and spicery.

scholarly journals Chemical Characterization and Antidiabetic Activity of Essential Oils from Pelargonium graveolens Leaves

2021 ◽  
Vol 9 (1) ◽  
pp. 109-113
Author(s):  
Javed Ahamad ◽  
Subasini Uthirapathy
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Study Finding ◽  
Antidiabetic Activity ◽  
Chemical Components ◽  
Diabetic Patients ◽  
Pelargonium Graveolens

Pelargonium graveolens (Geranium) is a source of the finest quality of fragrance and its essential oils are used as antibacterial, and antifungal agents. The aim of the current research is to determine chemical constituents in the essential oil of P. graveolens by GC-MS and evaluate its antidiabetic activity via α-glucosidase inhibition assay. The chemical composition of P. graveolens essential oil was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. GC-MS analysis determines 36 chemical components in the essential oil of P. graveolens leaves, and citronellyl isovalerate (10.41 %), menthol (9.61 %), linalool (8.63 %), p-menthone (6.31 %), and geranyl tiglate (4.99 %) were recorded as major constituents. The essential oil of P. graveolens leaves showed concentration dependant inhibition of α-glucosidase enzyme ranging from 28.13±1.41 to 74.24±2.53 µg/mL for concentration ranging from 31.25 to 1000 µg/mL. The IC50 values for of P. graveolens and acarbose were found as 93.72±4.76 and 80.4±2.17 µg/mL, respectively against the α-glucosidase enzyme. The study finding explores the chemical components of P. graveolens growing in the Iraqi Kurdistan region and scientifically supported its possible use in diabetic patients for controlling postprandial hyperglycemia.

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