scholarly journals Characterization by GC-MS of the Main Ethanolic Extract’S Components from the Bark of Talipariti elatum Sw. (Fryxell) Malvaceae

2018 ◽  
Vol 6 (2) ◽  
pp. 1
Author(s):  
José González ◽  
Armando Cuéllar ◽  
Enrique Gómez ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Quality Control ◽  
Crude Extract ◽  
Mass Spectra ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
First Time

Talipariti elatum (Sw.), commonly named Blue Mahoe, is used as antasthmatic, antitussive, against catarrh and expectorant in phytotherapy in Cuba, although the plants’ composition has not been determined in detail so far. An ethanolic extract from the bark of Talipariti elatum Sw. (Fryxell) Malvaceae that grows in Cuba was prepared by Soxhlet extraction with ethanol at 95% during 20 hours. The crude extract without purification was analyzed using a GCMS-QP2010 Ultra Shimadzu, and the components were identified by comparing their retention times and their mass spectra with those of NIST21Library. About 45 different chemical compounds were detected in the sample and from them, 31 chemical components were characterized and reported for the first time from Talipariti elatum. Our results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Talipariti samples.

scholarly journals GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

2018 ◽  
Vol 5 (4) ◽  
pp. 79 ◽  
Author(s):  
Pilar A. Soledispa ◽  
José González ◽  
Armando Cuéllar ◽  
Julio Pérez ◽  
Max Monan
Keyword(s):  
Mass Spectra ◽  
Chemical Constituents ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Analytical Technique ◽  
Main Components ◽  
First Time

A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of Smilax domingensis Wid. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

scholarly journals Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 898
Author(s):  
Xin Wang ◽  
Xiang-Jian Zhong ◽  
Na Zhou ◽  
Ning Cai ◽  
Jia-Hui Xu ◽  
...  
Keyword(s):  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Scientific Basis ◽  
Negative Ion ◽  
Chemical Components ◽  
Gymnadenia Conopsea ◽  
Orbitrap Mass Spectrometer ◽  
First Time ◽  
Orbitrap Ms

Gymnadenia conopsea R. Br. is a traditional Tibetan medicinal plant that grows at altitudes above 3000 m, which is used to treat neurasthenia, asthma, coughs, and chronic hepatitis. However, a comprehensive configuration of the chemical profile of this plant has not been reported because of the complexity of its chemical constituents. In this study, a rapid and precise method based on ultra-high performance liquid chromatography (UPLC) combined with an Orbitrap mass spectrometer (UPLC–Orbitrap–MS/MS) was established in both positive- and negative-ion modes to rapidly identify various chemical components in the tubers of G. conopsea for the first time. Finally, a total of 91 compounds, including 17 succinic acid ester glycosides, 9 stilbenes, 6 phenanthrenes, 19 alkaloids, 11 terpenoids and steroids, 20 phenolic acid derivatives, and 9 others, were identified in the tubers of G. conopsea based on the accurate mass within 3 ppm error. Furthermore, many alkaloids, phenolic acid derivates, and terpenes were reported from G. conopsea for the first time. This rapid method provides an important scientific basis for further study on the cultivation, clinical application, and functional food of G. conopsea.

scholarly journals Chemical Constituents from the Aerial Parts of Gynura Bicolor

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Jian Chen ◽  
Sven Mangelinckx ◽  
An Adams ◽  
Wei-lin Li ◽  
Zheng-tao Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Chemical Constituents ◽  
Pharmacological Study ◽  
Ethanolic Extract ◽  
Diabetic Mice ◽  
Aerial Parts ◽  
Treatment Of Diabetes ◽  
Aqueous Ethanolic Extract ◽  
Gynura Bicolor ◽  
First Time

Gynura bicolor (Willd.) DC., is used in folk recipes for the treatment of diabetes mellitus in Jiangsu, Zhejiang and Sichuan province in the south of China. A previous pharmacological study proved that the plant showed significant hypoglycemic activity on normal and alloxan-diabetic mice. In this study, two terpenes, four megastigmane-type norisoprenoids and two glycosides were isolated from the aqueous ethanolic extract of the aerial parts of Gynura bicolor and characterized mainly by NMR spectroscopy and mass spectrometry. These compounds were isolated for the first time from this plant, and no evidence could be found for the previous reported presence of megastigmane-type norisoprenoids in the genus Gynura.

scholarly journals Triterpenoid and Fatty Acid Contents from the Stem Bark of Cordia dichotoma (Forst f.)

Folia Medica ◽  
2018 ◽  
Vol 60 (4) ◽  
pp. 594-600 ◽  
Author(s):  
Pankajkumar B. Nariya ◽  
Vinay J. Shukla ◽  
R. N. Acharya ◽  
Mukeshkumar B. Nariya ◽  
Jayesh M. Dhalani ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chemical Constituents ◽  
Methyl Esters ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Chemical Compounds ◽  
Stem Bark ◽  
Gas Chromatography Mass Spectrometry ◽  
Medicinal Purpose ◽  
First Time

Abstract Aim: To isolate and determine the chemical constituents of the stem bark of Cordia dichotoma (Forst f.), a plant used for medicinal purpose in folk medicine. Materials and methods: Petroleum ether extract of the stem bark was used for this study. Saponification process was performed to separate fatty acid and unsaponifiable matter. Results: One triterpenoids, α-amyrin was isolated from the bark by using isocratic elution. The chemical compounds isolated, for the first time, were analyzed by GC/MS, IR, and UV. The chemical composition of the fatty acids methyl esters (FAMEs) in bark of Cordia dichotoma were also analyzed by gas chromatography-mass spectrometry. After methyl-esterification, 17 components were identified in the bark. The derivatization conditions were investigated in order to validate this method. Conclusion: The present analysis revealed that Cordia dichotoma stem bark contains 17 fatty acid. The principal themes of the review highlight the development and application of chromatographic techniques for the separation, isolation and detection of the compounds.

scholarly journals UPLC-QTOF/MS-Based Nontargeted Metabolomic Analysis of Mountain- and Garden-Cultivated Ginseng of Different Ages in Northeast China

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 33 ◽  
Author(s):  
Hailin Zhu ◽  
Hongqiang Lin ◽  
Jing Tan ◽  
Cuizhu Wang ◽  
Han Wang ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Chemical Components ◽  
Metabolomic Analysis ◽  
Multivariate Statistical ◽  
Similar Chemical ◽  
Different Ages ◽  
First Time

Aiming at further systematically comparing the similarities and differences of the chemical components in ginseng of different ages, especially comparing the younger or the older and mountain-cultivated ginseng (MCG), 4, 5, 6-year-old cultivated ginseng (CG) and 12, 20-year-old MCG were chosen as the analytical samples in the present study. The combination of UPLC-QTOF-MSE, UNIFI platform and multivariate statistical analysis were developed to profile CGs and MCGs. By the screening analysis based on UNIFI, 126 chemical components with various structural types were characterized or tentatively identified from all the CG and MCG samples for the first time. The results showed that all the CG and MCG samples had the similar chemical composition, but there were significant differences in the contents of markers. By the metabolomic analysis based on multivariate statistical analysis, it was shown that CG4–6 years, MCG12 years and MCG20 years samples were obviously divided into three different groups, and a total of 17 potential age-dependent markers enabling differentiation among the three groups of samples were discovered. For differentiation from other two kinds of samples, there were four robust makers such as α-linolenic acid, 9-octadecenoic acid, linoleic acid and panaxydol for CG4–6 years, five robust makers including ginsenoside Re1, -Re2, -Rs1, malonylginsenoside Rb2 and isomer of malonylginsenoside Rb1 for MCG20 years, and two robust makers, 24-hydroxyoleanolic acid and palmitoleic acid, for MCG12 years were discovered, respectively. The proposed approach could be applied to directly distinguish MCG root ages, which is an important criterion for evaluating the quality of MCG. The results will provide the data for the further study on the chemical constituents of MCG.

Determination of Compounds inside Inoculated Aquilaria malaccensis Leaves by Soxhlet Extraction

2015 ◽  
Vol 1113 ◽  
pp. 267-272 ◽  
Author(s):  
Ku Halim Ku Hamid ◽  
Nur Suhada Mustafar ◽  
Pazilah Mohd Yusof ◽  
Siti Sofia Mohammad Shawal ◽  
Mohibah Musa ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Specific Detail ◽  
The Earth ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
The World ◽  
Different Types

Aquilaria is a special tree that has been awarded to the earth. Aquilaria spp. which belongs to the family Thymelaeceae is one of the most precious trees is the world. Agarwood or gaharu is the name of the resin produced by Aquilaria spp. Historically, agarwood has widely been used as medicine. It was also being used for perfumes, incense and religious purposes in Muslim, Buddhist, Hindu, Christian, and Jewish societies. In order to discover the new uses of the agarwood, knowledge of the chemical constituents inside the plant need to be known. Aquilaria spp tree can be divided into two parts which is bark and leaves. Recently, several studies have been conducted to examine the chemical composition found in Aquilaria spp tree. However, there is still no specific detail documentation on the chemical compounds present in Aquilaria leaves. In this study, Soxhlet extraction method has been used to withdraw the compounds from the leaves. From the results obtained, it shows that extraction of compounds by using methanol was more effective. This was due to the higher polarity inside the methanol. Other than solvents, the samples preparation also gave an effect towards the final results. There were two different types of samples that have been used for this study which are fresh and dried A. malaccensis leaves. The obtaining results showed that more compounds have been extracted from the dried sample. It can be conclude that moisture content in the sample also plays a role in identification of compounds inside the A. malccensis leaves

scholarly journals Identification of Chemical Compounds from the Ethanolic Extract of the Bark of Bauhinia tomentosa L. By GC-MS Analysis

2016 ◽  
Vol 8 (03) ◽  
Author(s):  
Gopalakrishnan S. ◽  
Vadivel E.
Keyword(s):  
Mass Spectra ◽  
Skin Diseases ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Microbial Infections ◽  
Phytochemical Constituents ◽  
Major Chemical

The bark of Bauhinia tomentosa L. is used wildly used for the treatment of varies ailments such as inflammation, wound, dysentery, skin diseases and for microbial infections. In the present study the ethanolic extract of the bark of Bauhinia tomentosa L. has been subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of fourteen phytochemical constituents, These compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The major chemical constituents are 4-(Benzyloxy)-1-methoxy-2(3,7-dimethylocta-2,6-dienyl)-benzene (10.88%), Levodopa (6.39%) and (9E, 12E) - Methyl octadeca-9,12-dienoate (5.68%) which possess many biological activities. Hence these studies pave a platform to screen many bioactive components for wound healing.

scholarly journals Antioxidant Activity and Flavonoids Identification by LC-MS/MS Analysis in Leaf Extract from Trattinnickia rhoifolia Willd

2019 ◽  
Vol 8 (2) ◽  
pp. 13-34
Author(s):  
Lilian Marigo Magalhaes ◽  
Valeria Dornelles Gindri Sinhorin ◽  
Camila Cristina Pereira de Souza ◽  
Rogério de Campos Bicudo ◽  
Adilson Paulo Sinhorin
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Leaf Extract ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Glutathione S Transferase ◽  
Hypolipidemic Action ◽  
Protein Thiols ◽  
First Time

The objectives of this work were to evaluate the antioxidant and hepatoprotective activities in the oxidative stress model induced by paracetamol in male Swiss mice, to evaluate the hypoglycemic and hypolipidemic action and to identify flavonoids in the leaves ethanolic extract from Trattinnickia rhoifolia. By the LC-MS / MS method, eight flavonoids were identified in the hydromethanolic (HM) and ethyl acetate (EA) fractions, except for Amentoflavone, the flavonoids Apigenin, Canferol, Luteolin, Quercetin, Quercetin-3-β-d-glucoside, Rutin and Taxifoline were identified for the first time in this species. These fractions were evaluated for antioxidant capacity (DPPH— test) and their protective effect in vivo through the analysis of superoxide dismutase, catalase, glutathione-s-transferase, non-protein thiols, ascorbic acid, TBARS and carbonylated proteins. The data showed that EA has antioxidant capacity and superior oxidative stress repair in chemical and biological analyzes, besides hypoglycemic and hypolipidemic action. Thus, the present work contributes significantly to the literature, since it is the first study that identifies the chemical constituents and pharmacological properties of leaves extract from T. rhoifolia.

scholarly journals Chemical constituents of ethanol extract of leaves and molluscicidal activity of crude extracts from vitex trifolia linn.

2013 ◽  
Vol 59 (4) ◽  
pp. 19-32 ◽  
Author(s):  
Jeet S. Jangwan ◽  
Rita P. Aquino ◽  
Teresa Mencherini ◽  
Patrizia Picerno ◽  
Raghubir Singh
Keyword(s):  
Ursolic Acid ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Chloroform Extract ◽  
Biomphalaria Alexandrina ◽  
The Family ◽  
Hexane Extracts ◽  
First Time

Abstract β-sitosterol and two triterpenoids: ursolic acid acetate and platanic acid have been isolated from ethanolic extract of Vitex trifola leaves. β-sitosterol was previously isolated from the leaves, stem and seeds of Vitex trifolia. Ursolic acid acetate has been isolated for the first time in this plant species. Platanic acid has been reported for the first time in Vitex trifolia and even in the family of this plant: Verbenaceae. These compounds were characterized using spectroscopic methods including 1D-1HNMR, 13CNMR, ESIMS and 2D-NMR (HSQC, HMBC, COSY) experiments and confirmed by comparison of their NMR data with those from the literature. A preliminary molluscicidal test for ethanol, chloroform and n-hexane extracts of leaves of Vitex trifolia against Biomphalaria alexandrina adult snails showed that ethanol extract of leaves with LC50 value 26.42 mg/l (27.92 mg/l - 24.99 mg/l) was more effective than n-hexane extract with LC50 value 35.48 mg/l (43.81 mg/l - 28.72mg/l) and chloroform extract with LC50 value 46.77 mg/l (53.59 mg/l - 43.81 mg/l) after 24 h exposure.

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