scholarly journals Involvement of poly(ADP-ribose) polymerase-1 in Chinese patients with glioma: a potential target for effective patient care

2017 ◽  
Vol 33 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Jinhong Ren ◽  
Peilan Jia ◽  
Hongxia Feng ◽  
Xiuhua Li ◽  
Jinghui Zhao ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Sandwich Elisa ◽  
Chinese Patients ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Cytokine Levels ◽  
Potential Risk Factors ◽  
Elisa Technique ◽  
Polymerase Chain ◽  
Parp 1

Objective: We aimed to evaluate the genetic variation of poly(ADP-ribose) polymerase-1 (PARP-1) in the development of gliomas among Chinese individuals. Materials and methods: Patients with a confirmed diagnosis of glioma and healthy individuals with no clinical symptoms of glioma were enrolled at Liaocheng People’s Hospital, China. Genetic polymorphisms were studied in plasma samples by polymerase chain reaction-restriction fragment length polymorphism assay. Cytokine levels were measured routinely in serum samples by sandwich ELISA technique. Results: A total of 120 Chinese patients with gliomas and 120 healthy Chinese individuals were included. We found that patients with the GG genotype (odds ratio [OR] 2.53, 95% confidence interval [CI] 1.46-4.38, p<0.001) and carriers of the G allele (OR 11.5, 95% CI 6.31-21.3, p<0.0001) were at high risk of developing glioma. A del/ins polymorphism of the NF-κB1 gene (OR 4.27, 95% CI 2.43-7.50, p<0.001) was also found to be associated with glioma. In addition, significantly increased cytokine levels were observed in patients with glioma (p<0.05). Conclusions: Our findings showed that PARP-1 polymorphisms are involved in the development of glioma in Chinese individuals. Also serum cytokine levels can be considered among the potential risk factors for developing glioma.

Poly (ADP-ribose) polymerase-1 (PARP-1) in Chinese patients with Graves’ disease and Graves’ ophthalmopathy

2018 ◽  
Vol 96 (6) ◽  
pp. 556-561
Author(s):  
Tong Wu ◽  
Dong-run Tang ◽  
Liang Zhao ◽  
Feng-yuan Sun
Keyword(s):  
Clinical Symptoms ◽  
Sandwich Elisa ◽  
Chinese Patients ◽  
Graves Disease ◽  
Plasma Dna ◽  
Serum Samples ◽  
Graves Ophthalmopathy ◽  
Study Results ◽  
Department Of Ophthalmology ◽  
Parp 1

We aimed to evaluate the genetic variation of poly (ADP-ribose) polymerase-1 (PARP-1) as risk factor in development of Graves’ disease (GD) and Graves’ ophthalmopathy (GO) among Chinese individuals. Patients with confirmed diagnosis of GD or healthy individuals with no clinical symptoms of hyperthyroiditis were enrolled at the Department of Ophthalmology, Tianjin First Center Hospital, China. Genetic polymorphism was studied in plasma DNA samples of subjects by polymerase chain reaction of restriction fragment length polymorphism to confirm our hypothesis. Cytokine levels were measured routinely on serum samples of subjects by sandwich ELISA technique. Patients with GG genotype (odds ratio (OR) 95% CI = 2.25 (1.35–3.73), p = 0.002) and carriers of G allele (OR = 2.03 (1.23–3.36), p = 0.006) were at high risk of developing ophthalmopathy. Polymorphism of del/ins of nuclear factor-κB1 gene (NFkB1) gene (OR = 7.1 (2.88–17.52), p < 0.0001) and PARP-1 C410T polymorphism was found to be associated with GO (p < 0.05). Cytokine level was significantly higher in patients with GD (p < 0.05), but no significant change in cytokines level among GO patients from baseline (p > 0.05). Our study results recommended that polymorphism of PARP-1 gene is more likely responsible for development of GD in Chinese individuals. We also observed that the polymorphism of gene-related del/ins to NFkB1 in development of GO.

scholarly journals The Role of Anti-Endothelial Cell Autoantibodies and Immune Response in Acute Low-Tone Hearing Loss

2020 ◽  
pp. 014556132095250
Author(s):  
Diyan Chen ◽  
Zhujian Wang ◽  
Gaogan Jia ◽  
Huanyu Mao ◽  
Yusu Ni
Keyword(s):  
Hearing Loss ◽  
Endothelial Cell ◽  
Low Frequency ◽  
Serum Cytokine ◽  
Short Term ◽  
Serum Samples ◽  
Term Outcome ◽  
Short Term Outcome ◽  
Cytokine Levels ◽  
Low Tone

Objective: Immunity is associated with acute low tone hearing loss. However, the exact pathophysiology of immunity-mediated acute low tone hearing loss remains unknown. In this study, we evaluated the presence, therapeutic effectiveness, and immunopathological mechanisms of anti-endothelial cell autoantibodies (AECEs) in patients with acute low-frequency hearing loss. Material and Methods: Forty-nine patients who were treated as inpatients having acute low-frequency hearing loss and additional symptoms, such as ear fullness, tinnitus, dizziness, or hyperacusis, were enrolled in this study. Serum samples from these patients were collected for laboratory serum autoimmunity detection, including AECAs, antinuclear antibodies, immunoglobulin, and circular immune complex. Therapeutic responses to combination therapy in short-term outcome and serum cytokine levels were compared between AECA-positive and AECA-negative patients. Results: Anti-endothelial cell autoantibodies–positive patients tended to show significantly less response to standard therapy compared with AECAs controls ( P < .05). Moreover, some serum cytokine levels elevated in both AECAs− and AECAs+ groups. Positive ratio of interleukin-8 and concentrations of macrophage inflammatory protein-1α were found higher in AECAs+ groups ( P < .05). Conclusion: The results supported that AECAs might wield influence on the short-term outcome of acute low-tone hearing loss (ALHL) treatment. Furthermore, AECA-mediated acute low-frequency hearing loss possibly involved dysregulation of inflammation process and release of cytokines.

scholarly journals Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai’an, China

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Zilong Cheng ◽  
Mengda Liu ◽  
Peng Wang ◽  
Peng Liu ◽  
Meng Chen ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Clinical Symptoms ◽  
Shandong Province ◽  
Chain Reaction ◽  
Serum Samples ◽  
Fatal Disease ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Subcutaneous Edema ◽  
Polymerase Chain

Paratuberculosis, a chronic and sometimes fatal disease of ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, we examined paratuberculosis cases among 2–4-year-old dairy cows at farms in Shandong Province, China. Paratuberculosis cases were diagnosed based on clinical symptoms, pathological autopsy, and histopathological inspection. Characteristics of paratuberculosis in the affected dairy cattle included poor body condition, persistent diarrhea, subcutaneous edema, granulomatous ileitis (multibacillary), mesenteric lymphadenitis, and hepatitis. Acid-fast bacilli from fecal specimens and lymphocytes were putatively identified as MAP based on Ziehl-Neelsen staining, then confirmed using polymerase chain reaction-based testing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. Overall, only one MAP strain was isolated from a herd with symptomatic diarrhea. However, analysis of 586 serum samples from nine herds in Tai’an City revealed that 66.7% of herds and 14.2% of animals were seropositive for MAP. Our findings suggest that paratuberculosis is widely prevalent and therefore a significant threat to the dairy industry in Tai’an City, Shandong Province, China.

Effects of tylosin on serum cytokine levels in healthy and lipopolysaccharide-treated mice

2010 ◽  
Vol 58 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Ayse Er ◽  
Enver Yazar ◽  
Kamil Uney ◽  
Muammer Elmas ◽  
Feray Altan ◽  
...  
Keyword(s):  
Cytokine Production ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Cytokine Levels ◽  
Factor Α ◽  
Necrosis Factor ◽  
Different Doses

The effects of different doses of tylosin on serum cytokine concentrations were investigated in healthy and lipopolysaccharide-treated mice. The mice were divided into seven groups. Lipopolysaccharide (LPS) was injected into the positive control group. The other six groups received three different tylosin doses concurrently without or with LPS: 10 mg/kg, 100 mg/kg, 500 mg/kg, 10 mg/kg + LPS, 100 mg/kg + LPS and 500 mg/kg + LPS. After treatment, serum samples were collected at 0, 1, 2, 3, 6, 12 and 24 hours. Serum tumour necrosis factor α (TNFα), interleukin 1β (IL1β) and IL10 levels were determined by enzyme-linked immunosorbent assay (ELISA). Tylosin doses of 10 and 100 mg/kg induced no cytokine production in the healthy mice. Tylosin at 500 mg/kg had no effect on TNFα or IL1β production, but it induced IL10 production in healthy mice. All doses of tylosin reduced the elevated TNFα and IL1β in LPS-treated mice but increased their IL10 levels. In conclusion, these data suggest that tylosin has an immunomodulatory effect at the dose recommended for use against infection.

scholarly journals Evaluation of serum cytokine levels in recurrent airway obstruction

2016 ◽  
Vol 19 (4) ◽  
pp. 785-791 ◽  
Author(s):  
A. Niedźwiedź ◽  
H. Borowicz ◽  
K. Kubiak ◽  
J. Nicpoń ◽  
P. Skrzypczak ◽  
...  
Keyword(s):  
Airway Obstruction ◽  
Recurrent Airway Obstruction ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Th2 Response ◽  
Necrosis Factor Alpha ◽  
Reversible Airway Obstruction ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Ifn Γ

Abstract Recurrent airway obstruction (RAO) represents a serious health problem and is traditionally classified as an allergic disease, where contact with an antigen can induce clinical airway inflammation, bronchial hyper-responsiveness and reversible airway obstruction. Previous studies have demonstrated the presence of the Th2 response in the lungs of human patients with asthma and horses with heaves. These cells are involved in the production of cytokines which regulate the synthesis of immunoglobulins. 40 horses were evaluated: 30 horses with RAO and 10 healthy animals. The expression levels of interferon-alpha 1 (IFN-α1), interferon-gamma (IFN-γ), interleukin-1β, (IL-1β), IL-2, IL-4, IL-13 and tumor necrosis factor alpha (TNF-α) were measured in the serum obtained from control and RAO-susceptible horses during crisis. In all the patients, serum cytokine levels were detected. Serum median IL-13 and IFN-γ levels were significantly higher in RAO-affected horses than in the healthy group (p < 0.001). The serum median IFN-α1, IL-1β, IL-2, IL-4, and TNF-α levels were similar in both groups. These results indicate a low variability of the levels of cytokines and a high frequency of their detection in serum samples from horses with RAO. Immune mechanisms involved in equine RAO are more complex than those defined by a simple Th1/Th2 dichotomy.

scholarly journals An initial investigation of serum cytokine levels in patients with gadolinium retention

2020 ◽  
Vol 53 (5) ◽  
pp. 306-313
Author(s):  
Holden T. Maecker ◽  
Weiqi Wang ◽  
Yael Rosenberg-Hasson ◽  
Richard C. Semelka ◽  
Joseph Hickey ◽  
...  
Keyword(s):  
Linear Models ◽  
Elevated Serum ◽  
Serum Levels ◽  
Control Group ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Flu Vaccination ◽  
Gadolinium Retention ◽  
Cytokine Levels ◽  
The Difference

Abstract Objective: To determine whether individuals with proposed gadolinium deposition disease (GDD) have elevated serum levels of pro-inflammatory and pro-fibrotic cytokines, and whether specific cytokines are correlated with certain symptoms. Materials and Methods: Twenty-four participants recruited between May 2016 and June 2017 met GDD diagnostic criteria. The 64 control subjects provided serum samples before prophylactic flu vaccination. Serum cytokine levels were obtained with Luminex serum cytokine assay using eBiosciences/Affymetrix human 62-plex kits. Wilcoxon rank-sum tests were performed to assess the difference between the median fluorescence intensity values for the participants and the control group. Generalized linear models were built to evaluate the association between each cytokine of interest and selected participant symptoms. Results: Serum levels of 14 cytokines, including nine pro-inflammatory cytokines, were statistically significantly elevated compared to controls (p ≤ 0.05). Hypotheses regarding pro-fibrotic cytokines and cytokine links to specific symptoms’ intensity were not confirmed. Conclusion: The statistically significantly elevated cytokines may be markers of susceptibility to GDD or agents of symptom induction. These findings suggest that individuals developing symptoms characteristic of GDD after a contrast-assisted magnetic resonance imaging should be studied to investigate whether gadolinium retention and elevated cytokines may be related to their symptoms.

scholarly journals Analysis of common mutation for GALT gene in newborns with galacatosemia Nineveh governorate

2014 ◽  
Vol 8 (1) ◽  
pp. 41-45
Author(s):  
Owayes M Alhassani ◽  
Akeel H. Alassie ◽  
Saad G. Saleh
Keyword(s):  
Allele Frequency ◽  
Clinical Symptoms ◽  
Molecular Study ◽  
Control Group ◽  
Common Mutation ◽  
Classical Galactosemia ◽  
Galt Gene ◽  
Elisa Technique ◽  
Polymerase Chain ◽  
N314d Mutation

Iraq contains many diseases that have never been counted or examined, including diseases related to food, which has deteriorated in recent years, and has rapid and direct impact especially on the children category, one of these diseases is galactosemia. Classical galactosemia, deficiency of galac tose-1-phosphate uridyltransferase GALT, is characterized by acute symptoms of hepatomegaly, jaundice, sepsis, cataract, vomiting, and diarrhea and growth retardation. Our previous molecular study showed that the most common mutation of the GALT gene is a missense mutation of Q188R (replacement of glutamine-188 by arginine in exon 6 and N314D mutation replacement of aspargen-314 by aspartic acid) in exon 10. The aim of this study was to determine the possibility of diagnosing galacatosemia, and to search for galactosemia mutation Q188R and N314D in Iraqi population. Blood samples were collected from babies admitted to the children's hospitals in Mosul City depending on the clinical symptoms of disease and then serum was taken. Measuring the Galactose-1-Phosphate uridylytransferase GALT enzyme activity and galactose -1- phosphate in serum by ELISA technique was done. DNA samples were analyzed by the polymerase chain reaction followed by digestion with restriction endonuclease HpaΙΙ and AvaΙΙ for Q188R and N314D mutation. The results showed a significant decrease in the level of the GALT enzyme in children with galactosemia 21.7 ± 0.45 and among non-diagnosed children 79.93 ± 1.44 compared with control group 160.33 ± 0.93 as well as a significant decrease in the level of the enzyme among mothers 20.5 ± 1.92 was observed. Gal-1-P level was significantly higher in the cases than that of the control group, while that of the not diagnosed children and mothers groups showed inconsistent difference. Also the result showed absence allele frequency for Q188R mutation and present allele frequency for N314D mutation in Iraqi population. In conclusions It is possible to depend on measurement of Galactose-1-Phosphate as indicator in the diagnosis of Galactosemia in newborn, the main mutation in GALT gene causes galactosemia is N314D in Iraqi population.

scholarly journals Comparative Clinical Evaluation of the Roche Elecsys and Abbott Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Serology Assays for Coronavirus Disease 2019 (COVID-19)

2020 ◽  
Vol 145 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Shaun S. Tan ◽  
Sharon Saw ◽  
Ka Lip Chew ◽  
Cindy Wang ◽  
Anastacia Pajarillaga ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Clinical Symptoms ◽  
Test Results ◽  
Serum Samples ◽  
False Negatives ◽  
Antiretroviral Medication ◽  
Polymerase Chain ◽  
Negative Controls ◽  
Level Of Agreement ◽  
Abbott Architect

Context.— The use of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic tests detects antibodies in the host, contributing to the identification of individuals who have been exposed to coronavirus disease 2019 (COVID-19). Objective.— To critically evaluate 2 commercially available SARS-CoV-2 serology tests. Design.— A total of 333 unique, nonduplicated serum samples obtained from COVID-19 patients (n = 170) and negative controls (n = 163) obtained before December 2019 were used in the study. Samples were tested on the Roche E411 and Abbott Architect i4000SR platforms, and results were correlated to reverse transcription polymerase chain reaction (PCR) results and clinical symptoms. Results.— There was a strong level of agreement in the qualitative results between both assays, with a Cohen κ value of .840, P &lt; .001. The specificity for both Roche and Abbott were excellent at 100%. Roche exhibited marginally better performance in the 21 days or more group with a sensitivity of 90.6% (95% CI, 75.8%–96.8%) versus an Abbott sensitivity of 84.4% (95% CI, 68.3%–93.1%), as well as in the 14- to 20-day group with a sensitivity of 85.7% (95% CI, 65.4%–95.0%) versus an Abbott sensitivity of 81.0% (95% CI, 60.0%–92.3%). Less than 14 days of symptoms groups exhibited poor sensitivity at less than 50% for both assays. The areas under curve (± standard error) for Roche (0.894 ± 0.025, P &lt; .001) and Abbott (0.884 ± 0.026, P &lt; .001) were very similar. Potential confounders for negative serologic results include antiretroviral medication use and pauci-symptomatic patients. Conclusions.— Specificities for high-throughput Roche and Abbott immunoassays are excellent, but users need to be cautious to interpret serologic test results after 14 days of symptoms to avoid false negatives.

scholarly journals Cytokine, Chemokine, and Metalloprotease Activation in the Serum of Patients with Nephropathia Epidemica from the Republic of Tatarstan and the Republic of Mordovia, Russia

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 527
Author(s):  
Ekaterina Martynova ◽  
Yuriy Davidyuk ◽  
Emmanuel Kabwe ◽  
Ekaterina E. Garanina ◽  
Venera Shakirova ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Hemorrhagic Fever ◽  
Human Infection ◽  
Nephropathia Epidemica ◽  
Matrix Metalloproteases ◽  
Genetic Lineage ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Leukocyte Chemotaxis ◽  
The Republic

Nephropathia Epidemica (NE), endemic to several Volga regions of Russia, including the Republic of Tatarstan (RT) and the Republic of Mordovia (RM), is a mild form of hemorrhagic fever with renal syndrome caused by infection with rodent-borne orthohantaviruses. Although NE cases have been reported for decades, little is known about the hantavirus strains associated with human infection in these regions. There is also limited understanding of the pathogenesis of NE in the RT and the RM. To address these knowledge gaps, we conducted comparative analyses of patients with NE in the RT and the RM. Clinical symptoms were more severe in patients with NE from the RM with longer observed duration of fever symptoms and hospitalization. Analysis of patient sera showed changes in the levels of numerous cytokines, chemokines, and matrix metalloproteases (MMPs) in patients with NE from both the RT and the RM, suggesting leukocyte activation, extracellular matrix degradation, and leukocyte chemotaxis. Interestingly, levels of several cytokines were distinctly different between patients NE from the RT when compared with those from the RM. These differences were not related to the genetic variation of orthohantaviruses circulating in those regions, as sequence analysis showed that Puumala virus (PUUV) was the causative agent of NE in these regions. Additionally, only the “Russia” (RUS) genetic lineage of PUUV was detected in the serum samples of patients with NE from both the RT and the RM. We therefore conclude that differences in serum cytokine, chemokine, and MMP levels between the RT and the RM are related to environmental factors and lifestyle differences that influence individual immune responses to orthohantavirus infection.

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