scholarly journals Using polynomial modeling for calculation of sperm quality parameters in CASA

2021 ◽  
Author(s):  
Fatih OKUMUŞ ◽  
Fatih KOCAMAZ ◽  
Mustafa Erkan ÖZGÜR
Keyword(s):  
Sperm Quality ◽  
Quality Parameters ◽  
Polynomial Modeling

scholarly journals Sperm quality parameters are increased and asymmetric in house mouse hybrids

2019 ◽  
Author(s):  
Iva Martincová ◽  
Ľudovít Ďureje ◽  
Stuart J. E. Baird ◽  
Jaroslav Piálek
Keyword(s):  
Y Chromosome ◽  
House Mouse ◽  
Sperm Quality ◽  
Quality Parameters ◽  
F1 Hybrids ◽  
Secondary Contact ◽  
Male Fitness ◽  
Cross Direction ◽  
Sperm Trait ◽  
The Difference

AbstractSpermatogenesis is a tuned cascade of processes producing sperm; impairment of any phase of this process can affect fitness of males. The level of impairment can be pronounced in hybrids between genetically divergent populations. To explore the effect of hybridization on sperm quality we produced F1 hybrids from 29 wild derived strains of two house mouse subspecies, M. m. musculus and M. m. domesticus, which diverged 0.5 MY ago. The measured sperm quality traits did not significantly differ between intrasubspecific crosses. Effects of intersubspecific hybridization were dependent on sperm trait and cross direction. The proportion of sperm head abnormalities was increased in F1 intersubspecific hybrids. The frequency of dissociated sperm heads was increased in the M. m. musculus × M. m. domesticus (♀×♂) F1 but decreased in M. m. domesticus × M. m. musculus (♀×♂) F1 hybrids, with the difference in medians being more than 180%. We deduce that the dissociated sperm heads trait is associated with the X chromosome and modulated by interaction with the Y chromosome; nevertheless, the high proportion of unexplained variance (55.46 %) suggests the presence of polymorphic autosomal interactions. The reported differences in sperm quality between cross types may be highly relevant to male fitness in zones of secondary contact between the two subspecies. The cross direction asymmetry in frequency of dissociated sperm heads should favour the M. m. musculus Y chromosome. This is consistent with the spread of the M. m. musculus Y chromosome in nature across the hybrid zone between these two subspecies.

scholarly journals Evaluating bull fertility based on non-return method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 27-40
Author(s):  
Igor Prka ◽  
Dragan Vukovic ◽  
Stevan Perkovic
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Service Period ◽  
Microscopic Evaluation ◽  
Live Sperm ◽  
Return Method ◽  
The Impact ◽  
First Time

In order to evaluate the results of reproductive cows and heifers, different parameters of fertility are used, such as the service period, insemination index, intercalving time and others, and of the breeding bulls the values obtained through non-return. An ejaculate is taken up for further processing by veterinary centres only provided it meets the prescribed quality parameters. Rating semen parameters includes a macroscopic (volume, colour, consistency, smell and pH) and a microscopic evaluation (mobility, density, percentage of live sperm and abnormal and damaged sperm). In addition to sperm quality and the fertility of the female animal, the results of the non-return method are also influenced by a number of exogenous causes (season, age, race, insemination techniques) that have no small impact on the end result of insemination - pregnancy. In order to obtain more objective results of the fertility of bulls the following tasks were undertaken, namely: 1. to calculate with the non-return method the fertility of bulls in over 10,000 cows inseminated for the first time during a period of 6 years; and 2. to analyze the impact of semen quality, season, age of cow and bull, and the bull breed on the results of fertility.

Fluoxetine treatment of prepubertal male rats uniformly diminishes sex hormone levels and, in a subpopulation of animals, negatively affects sperm quality

2018 ◽  
Vol 30 (10) ◽  
pp. 1329 ◽  
Author(s):  
María E. Ayala ◽  
Ayari Gonzáles ◽  
Rodrigo M. Olivarez ◽  
Andrés Aragón-Martínez
Keyword(s):  
Adult Stage ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Graphical Analysis ◽  
Quality Parameters ◽  
Male Rats ◽  
Serum Concentrations ◽  
Sperm Density ◽  
Sexual Steroids ◽  
Fluoxetine Treatment

Fluoxetine (Flx) is a selective serotonin reuptake inhibitor that alters the male reproductive system when administered at the adult stage or after maternal exposure. In the present study we evaluated the effects of Flx administration on reproductive parameters during juvenile–peripubertal development when treated male rats reached adulthood. Groups of rats were treated daily with Flx (5 mg kg−1, i.p.) or saline (0.9% NaCl), or were left untreated. Rats were treated between 30 and 53 days of age and were killed at 65 days of age. Serotonin concentrations were determined in the hypothalamus, hypophysis and testis. Gonadotrophins, sex steroids and sperm quality (membrane integrity, sperm with functional mitochondria, sperm density, sperm motility and morphological abnormalities) were also evaluated. Flx did not affect bodyweight, but significantly diminished LH, FSH, progesterone and testosterone serum concentrations. After graphical analysis, a subgroup of rats was identified whose sperm quality parameters were greatly affected by Flx. In the present study we show that Flx administered to juvenile rats disrupts the hypothalamic–hypophyseal–testicular axis and its effects on sperm quality are not homogeneous in adults. In contrast, Flx altered concentrations of gonadotrophins and sexual steroids in all treated rats. These results suggest caution should be exercised in the prescription of Flx to prepubertal males.

scholarly journals Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)

2016 ◽  
Vol 53 (3) ◽  
pp. 223-232 ◽  
Author(s):  
Yi-Chen Chen ◽  
Hsiu-Chou Liu ◽  
Liang-Yuan Wei ◽  
Jeng-Fang Huang ◽  
Chai-Ching Lin ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Quality Parameters ◽  
Reproductive Efficiency ◽  
Muscovy Duck ◽  
Cairina Moschata

scholarly journals Finding an Effective Freezing Protocol for Turkey Semen: Benefits of Ficoll as Non-Permeant Cryoprotectant and 1:4 as Dilution Rate

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 421 ◽  
Author(s):  
Michele Di Iorio ◽  
Giusy Rusco ◽  
Roberta Iampietro ◽  
Maria Antonietta Colonna ◽  
Luisa Zaniboni ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Dna Integrity ◽  
Osmotic Resistance ◽  
Progressive Motility ◽  
Nitrogen Vapor ◽  
Combined Use ◽  
Cryopreservation Protocol

The present study aimed to find an effective cryopreservation protocol for turkey semen through the combined use of dimethylsulfoxide (DMSO) and three non-permeant cryoprotectants (NP-CPAs), sucrose, trehalose, and Ficoll 70. In addition, the action of two dilution rates (1:2 and 1:4) were also investigated. Semen was processed according to two final dilution rates and the following treatments: Tselutin extender (TE)/DMSO (control), TE/DMSO + sucrose or trehalose 50, 100, 200, or 400 mM, and TE/DMSO + Ficoll 0.5, 0.75, 1, or 1.5 mM. In total 26 different combinations treatments were achieved. The diluted semen was filled up into straws and frozen on liquid nitrogen vapor. The post-thawing sperm quality was assessed by analyzing motility, membrane integrity, osmotic resistance, and DNA integrity. The results obtained revealed a significant effect of NP-CPA concentration on total and progressive motility, on most of the kinetic parameters, on membrane integrity and DNA integrity, while the post-thaw quality was less affected by dilution rate. The highest post-thaw quality for all sperm quality parameters assessed except curvilinear velocity (VCL) and DNA integrity were found in semen frozen with 1 mM Ficoll/1:4 (p < 0.05). Our findings provide an important contribution for the identification of a reference procedure for turkey semen cryopreservation, in order to create the first national avian semen cryobank.

scholarly journals The use of insulin-like growth factor 1 improved the parameters of the seminogram in a patient with severe oligoasthenoteratozoospermia

2019 ◽  
Vol 7 ◽  
pp. 2050313X1983415
Author(s):  
José Arturo Mora Rodríguez ◽  
Leonardo M Porchia ◽  
Felipe Camargo ◽  
Esther López-Bayghen
Keyword(s):  
Growth Factor ◽  
Assisted Reproductive Technologies ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Reproductive Technologies ◽  
Quality Parameters ◽  
Intradermal Injection ◽  
Sperm Parameters ◽  
Insulin Like Growth Factor ◽  
Progressive Motility

Male patients suffering from oligoasthenoteratozoospermia typically failed to achieve pregnancy, even with assisted reproductive technologies. Growth hormone and insulin-like growth factor 1 have been shown to regulate sperm quality parameters; therefore, the insulin-like growth factor 1 supplement could improve sperm parameters. Here, we determine the effect insulin-like growth factor 1 has on sperm parameters in a patient suffering from oligoasthenoteratozoospermia. A 47-year-old male was administered once a day 1.5 IU of insulin-like growth factor 1 by intradermal injection for 2 months. Seminogram analysis was performed before and after. Treatment with insulin-like growth factor 1 resulted in a 15.5-fold improvement in sperm concentration (1.1 × 106 vs 18.3 × 106 per mL), 71.4% change in volume (0.7 vs 1.2 mL), increased progressive motility (2% vs 43%), and the total volume of sperm with progressive motility (0% vs 23.6%). Here, we show that administering a daily dose of insulin-like growth factor 1 can improve sperm quality parameters.

scholarly journals Effect of increasing equilibration time of diluted bull semen up to 72 h prior to freezing on sperm quality parameters and calving rate following artificial insemination

2018 ◽  
Vol 108 ◽  
pp. 217-222 ◽  
Author(s):  
E.M. Murphy ◽  
B. Eivers ◽  
C.M. O'Meara ◽  
P. Lonergan ◽  
S. Fair
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Equilibration Time ◽  
Bull Semen ◽  
Calving Rate

scholarly journals Inhibitors of zinc-dependent metalloproteases hinder sperm passage through the cumulus oophorus during porcine fertilization in vitro

Reproduction ◽  
2012 ◽  
Vol 144 (6) ◽  
pp. 687-697 ◽  
Author(s):  
J Beek ◽  
H Nauwynck ◽  
D Maes ◽  
A Van Soom
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Inhibitory Effect ◽  
Fertilization Rate ◽  
Quality Parameters ◽  
Fertilization In Vitro ◽  
Cumulus Oophorus ◽  
Sperm Penetration

In this study, we report for the first time on a possible contribution of metalloproteases in sperm passage through the cumulus matrix in pigs. The presence of 20 μM 1,10-phenanthroline (1,10-PHEN), inhibitor of zinc-dependent metalloproteases, strongly inhibited the degree of sperm penetration in cumulus-intact (CI), but not in cumulus-free (CF), porcine oocytes during IVF. The inhibitory effect of 1,10-PHEN was due to the chelation of metal ions as a non-chelating analog (1,7-PHEN) did not affect IVF rates. Furthermore, incubation with 1,10-PHEN did not affect sperm binding to the zona pellucida nor sperm motility, membrane integrity, or acrosomal status. These findings led to the assumption that 1,10-PHEN interacts with a sperm- or cumulus-derived metalloprotease. Metalloproteases are key players in physiological processes involving degradation or remodeling of extracellular matrix. In vivo, their proteolytic activity is regulated by tissue inhibitors of metalloproteases (TIMP1–TIMP4). We tested the effect of TIMP3 on fertilization parameters after porcine IVF. Similar to 1,10-PHEN, TIMP3 inhibited total fertilization rate of CI but not CF oocytes and did not influence sperm quality parameters. Although the inhibitory effect was stronger in CI oocytes, TIMP3 also reduced the degree of sperm penetration in CF oocytes, suggesting the involvement of a metalloprotease in a subsequent step during fertilization. In conclusion, our results indicate the involvement of TIMP3-sensitive, zinc-dependent metalloprotease activity in sperm passage through the cumulus oophorus in pigs. The results should provide the basis for further biochemical research toward the localization and identification of the metalloprotease involved.

Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

2015 ◽  
Vol 12 (2) ◽  
Author(s):  
Okukwe Obode ◽  
Oluwatoyin Okafor ◽  
Ochuko Erukainure ◽  
Atinuke Ajayi ◽  
Yewande Suberu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Antioxidant Activities ◽  
Reduced Glutathione ◽  
Sperm Quality ◽  
Day Treatment ◽  
Quality Parameters ◽  
Diabetic Rats ◽  
Testicular Toxicity ◽  
Cervical Dislocation

Abstract: The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated.: Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality.: Induction of diabetes led to a significant (p<0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p<0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p<0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level.: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

Use of colloid single layer centrifugation for dromedary camel semen: Effect of initial dilution and comparison of two colloids on sperm quality parameters

2016 ◽  
Vol 169 ◽  
pp. 123 ◽  
Author(s):  
C. Malo ◽  
J.M. Morrell ◽  
E.G. Crichton ◽  
B.S. Pukazhenthi ◽  
J.A. Skidmore
Keyword(s):  
Sperm Quality ◽  
Single Layer ◽  
Quality Parameters ◽  
Dromedary Camel ◽  
Initial Dilution
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