scholarly journals Atlantic seabob shrimp as biomonitor of Cu and Zn near port activities: is it really a suitable choice?

2021 ◽  
Vol 56 (4) ◽  
pp. 665-672
Author(s):  
Ana Paula Madeira Di Beneditto ◽  
Keltony De Aquino Ferreira ◽  
Braulio Cherene Vaz de Oliveira ◽  
Carlos Eduardo De Rezende ◽  
Inácio Abreu Pestana
Keyword(s):  
Trophic Position ◽  
Suitable Choice ◽  
Maturity Stage ◽  
Clear Trend ◽  
Shrimp Species ◽  
Southeast Brazil ◽  
Foraging Area ◽  
Freeze Dried ◽  
Xiphopenaeus Kroyeri ◽  
Cu And Zn

The trace elements concentration in the muscle of the Atlantic seabob shrimp (Xiphopenaeus kroyeri) caught in coastal fishing highlighted copper (Cu) and zinc (Zn), both related to antifouling systems, as the main elements related to the intensity of port activities of southeast Brazil (~20—to 22ºS). The aim of this study is to analyze if the behavior of Cu and Zn in the muscle of this shrimp species is constant among different sampling sites, verifying if the species is suitable as biomonitor for these elements. The shrimps came from fisheries done in 2017 in Vitória, Anchieta, and Farol de São Tomé, southeast Brazil. After sampling, each individual was categorized for gender and maturity stage, measured, and weighted. Bulk muscle samples were freeze-dried for determination of Cu, Zn, and ratios of stable isotopes of carbon (δ13C) and nitrogen (δ15N). The data analysis verified if the concentration of Cu and Zn in male and female shrimps vary among maturity stages and sampling sites, and how the concentration of Cu and Zn is related to shrimps foraging area and/or trophic position. Both bioaccumulation and growth dilution occurred, but not in the same way for genders and sampling sites, with Cu showing more variability. Relationships between elements and shrimps foraging area and trophic position did not show a clear trend among the sampling sites. Regression models indicated moderate relationships, explaining 51% (Cu) and 60% (Zn) of the association with the foraging area in Anchieta, but up to 8% in Vitória and Farol de São Thomé. For the trophic position, the models explained 33% (Cu) and 34% (Zn) in Anchieta and up to 14% in Vitória and Farol de São Thomé. The results showed that the utilization of this shrimp species as biomonitor of marine coastal environments near port activities to monitoring the levels of Cu and Zn is not a suitable choice, at least in the spatial scale considered by this study.

Interlaboratory Comparison of Analyses for Heavy Metals in Clam Tissue

1986 ◽  
Vol 69 (5) ◽  
pp. 863-868
Author(s):  
Marilyn R Hendzel ◽  
Bruce W Fallis ◽  
Brigitte G E Demarch
Keyword(s):  
Heavy Metals ◽  
Coefficient Of Variation ◽  
Interlaboratory Comparison ◽  
Interlaboratory Study ◽  
Oyster Tissue ◽  
Coefficients Of Variation ◽  
Freeze Dried ◽  
Lead Levels ◽  
Clam Tissue ◽  
Cu And Zn

Abstract Results are reported for an interlaboratory study conducted to assess the reproducibility of analyses for lead, copper, cadmium, and zinc. The 10 participating laboratories analyzed 2 samples of freeze-dried clam tissue and 1 disguised sample of NBS Oyster Tissue. Interlaboratory variations were observed for all metals, although the methods yielded reproducible data for Cu and Zn with average interlaboratory coefficients of variation of 15 and 11%, respectively. The performance of methods used for Cd and, more so, for Pb was less than satisfactory. Cadmium levels in the 3 samples ranged from about 0.7 to 3.7 ppm with an average interlaboratory coefficient of variation (CV) of 24%. Lead levels in the 3 samples were about 0.5 ppm with an interlaboratory CVof 68%. Some laboratories' results were consistently high or low but data were insufficient to relate these trends to one particular variable. Results from this study were compared with 5 other studies reported in the literature since 1980. Coefficients of variation from all studies were comparable for samples with similar metal concentrations

scholarly journals Quantitative Determination of Phenolic Acids and Flavonoids in Fresh Whole Crop Rice, Silage, and Hay at Different Harvest Periods

2020 ◽  
Vol 10 (22) ◽  
pp. 7981
Author(s):  
Palaniselvam Kuppusamy ◽  
Dahye Kim ◽  
Hyung Soo Park ◽  
Jeong Sung Jung ◽  
Ki Choon Choi
Keyword(s):  
Ferulic Acid ◽  
Caffeic Acid ◽  
Phenolic Acid ◽  
Array Detector ◽  
Maturity Stage ◽  
Coumaric Acid ◽  
Plant Metabolites ◽  
Fresh Freeze ◽  
Freeze Dried ◽  
Phenolic And Flavonoid

Whole crop rice (WCR) is used as an important feed for livestock ruminants. In this study, “Yeongwoo” variety WCR (cultivated on the Korean peninsula) was harvested at three different maturity stages (booting, heading, and milk) and their phenolic acid and flavonoid profiles in the lactic acid bacteria (LAB)-inoculated and noninoculated silage, hay, and fresh freeze-dried WCR extract were quantified. The alterations in the phenolic and flavonoid contents of the selected WCR during maturation in different samples were analyzed by the High-Performance Liquid Chromatography- Diode Array Detector (HPLC–DAD) technique. The six phenolics (caffeic acid, ferulic acid, p-coumaric acid, chlorogenic acid, dihydroxy benzoic acid, and prophyl gallate) and six flavonoids (rutin hydroxide, luteolin, kaempferol, vitexin, myricetin, and quercetin) were noted to have slight differences between the LAB-inoculated and noninoculated silage samples; however, the phenolics and flavonoids were higher in hay WCR at the milk stage compared to the silage and fresh freeze-dried samples. The results indicate that WCR harvest times have different phenolic compounds in the WCR silage, hay, and fresh samples. The phenolic and flavonoid compounds were higher (p < 0.05) with the increase in maturity (Stage 1–3). The stage of WCR maturity was positively related (p < 0.05) to the amount of phenolic acid and flavonoid contents (µg/g) in hay, silage, and fresh freeze-dried extract. We observed the WCR had high amounts of phenolic acid and flavonoid concentrations at milk stage (Stage 3) hay (quercetin, kaemferol, luteolin, ferulic acid, caffeic acid, and coumaric acid were 1.28, 1.29, 0.54, 1.54, 1.92, and 1.81 µg/g, respectively) compared with the booting and heading stages (Stages 1 and 2), with acceptable accuracy on a pilot scale. Based on these results, it could be concluded that LAB (Lactobacillus plantarum)-inoculated whole crop rice silage (WCRS) did not affect the phenolics and flavonoids of secondary plant metabolites in fermented silage. However, phenolics and flavonoids were of higher rations in WCR at the milk stage. Furthermore, this phenolic acid and flavonoid effect needs to be confirmed using large-scale in vivo analysis.

Levels of stored energy but not marine foraging patterns differentiate seasonal ecotypes of wild and hatchery steelhead (Oncorhynchus mykiss) returning to the Kalama River, Washington

2017 ◽  
Vol 74 (2) ◽  
pp. 157-167 ◽  
Author(s):  
James S. Lamperth ◽  
Thomas P. Quinn ◽  
Mara S. Zimmerman
Keyword(s):  
Stable Isotopes ◽  
Oncorhynchus Mykiss ◽  
Trophic Position ◽  
Stored Energy ◽  
Arrival Date ◽  
Migration Distance ◽  
Foraging Patterns ◽  
Hatchery Fish ◽  
Foraging Area ◽  
Sufficient Energy

Anadromous fishes need to store sufficient energy at sea to migrate and reproduce, but the energetic demands of freshwater migration distance have been difficult to distinguish from the demands of fasting in fresh water prior to spawning. In addition, differences in stored energy may result from differences in metabolic storage or marine diet. We estimated somatic lipids (SL) and stable isotopes of N and C (as indices of marine trophic position and feeding location) in adult wild and hatchery steelhead (Oncorhynchus mykiss) of summer (stream-maturing) and winter (ocean-maturing) runs that migrate similar distances to spawn. We found that SL at return depended on several factors. Summer steelhead had twice the SL as winter steelhead, and within each run SL decreased with arrival date, converging on about 1% SL. In summer steelhead, females had 19% more SL than males, and wild fish had 21% more SL than hatchery fish. In winter steelhead, females had 27% less SL than males, and there was no difference between rearing origins. No differences were detected in δ15N or δ13C between ecotypes or rearing origins. Taken together, the results indicated different patterns of energy storage among and within ecotypes despite no apparent differences in marine trophic position or foraging area.

scholarly journals Evaluation of the Antioxidant Activity of Bignay (Antidesma bunius (Linn.) Spreng var. Kalabaw) Flesh and Seeds as Affected by Maturity and Processing Method

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Kristel June Sartagoda ◽  
Ma. Cristina Ilano ◽  
Lloyd Earl Flandez ◽  
Katherine Ann Castillo-Israel
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Processing Method ◽  
Total Phenolic ◽  
Maturity Stage ◽  
Anthocyanin Content ◽  
Processing Methods ◽  
Total Anthocyanin ◽  
Antioxidant Content ◽  
Freeze Dried

This study aimed to determine the influence of maturity stages and processing methods (blanching and steaming) on the antioxidant profile and in vitro antioxidant activities of bignay (Antidesma bunius (Linn.) Spreng var. Kalabaw) flesh and seeds. Bignay fruits of three maturities (unripe, half ripe, and fully ripe) were collected from Laguna, Philippines. Each maturity stage was subdivided into three lots. One lot underwent blanching at 90 ± 5°C for 2 minutes, and another underwent steaming at 105 ± 5°C for 5 minutes while the last did not undergo treatment. Seeds of the samples were then separated from the flesh. Both seeds and flesh were freeze-dried, extracted, and analyzed for antioxidant contents (total phenolic content, total flavonoid content, and total anthocyanin content) and antioxidant activity by DPPH, ABTS, and FRAP assays. Results show that both the maturity and processing methods significantly affect the antioxidant content and activity of the samples. Moreover, except for the FRAP assay done on flesh samples, all assays showed that there is significant interaction between the effect of maturity and processing method on the antioxidant contents and activity of bignay flesh and seeds. Results also show that fully ripe flesh and seeds yielded greater antioxidant content and antioxidant activity than their half-ripe and unripe counterparts; whereas, blanched flesh and seeds generally had higher antioxidant activities than their unprocessed and steamed counterparts.

Foraging area, not trophic position, is linked to head size variation in adult female loggerhead turtles

2017 ◽  
Vol 302 (4) ◽  
pp. 279-287 ◽  
Author(s):  
J. T. Price ◽  
J. B. Pfaller ◽  
H. B. Vander Zanden ◽  
K. L. Williams ◽  
A. B. Bolten ◽  
...  
Keyword(s):  
Adult Female ◽  
Trophic Position ◽  
Size Variation ◽  
Head Size ◽  
Foraging Area ◽  
Loggerhead Turtles

scholarly journals Antioxidant Activity and Phytochemical Content of Fresh and Freeze-Dried Lepisanthes fruticosa Fruits at Different Maturity Stages

2017 ◽  
Vol 9 (2) ◽  
pp. 147 ◽  
Author(s):  
Mirfat Ahmad Hasan Salahuddin ◽  
Zaulia Othman ◽  
Joanna Cho Lee Ying ◽  
Erny Sabrina Mohd Noor ◽  
Salma Idris
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Maturity Stage ◽  
Phytochemical Content ◽  
Fruit Extracts ◽  
Maturity Stages ◽  
Freeze Dried ◽  
Ic50 Values ◽  
Stage 1

Antioxidant and phytochemical compounds of fruits can vary widely depending on many factors such as processing and maturity stage as one of the major contributors. Therefore, this study investigated the antioxidant activity and phytochemical attributes of fresh and freeze-dried Lepisanthes fruticosa fruit extracts at eight different maturity stages. The freeze-dried extracts were obtained by lyophilisation using a freeze-dryer. In general, antioxidant activity and phytochemical contents of both fresh (FLF) and freeze-dried (FDLF) extracts showed a decrease with fruit maturation. Among the eight maturity stages developed for L. fruticosa, the lower maturity (unripe) stages exhibited the strongest potential, with stage 1 being the most notable. The FDLF fruit extracts were found to be significantly (P < 0.05) stronger radical scavenger than FLF extracts at all maturity stages tested. The IC50 values of FDLF for the eight maturity stages were more effective, with stage 1 showing the lowest IC50 (1.57 mg/ml). Total phenolic content of FDLF was also significantly (P < 0.05) higher than FLF at all eight stages tested, with the highest also being shown at stage 1 (15848.96 ± 401.82 mg/100 g). On the contrary, FLF extracts displayed significantly (P < 0.05) higher total flavonoid content than FDLF at almost all stages except for 2, 3 and 6. The highest content was shown in stage 1 with 37.35 ± 0.77 mg/100 g. These findings showed that antioxidant activity and phytochemical content of L. fruticosa fruits were significantly affected by processing and maturity. The obtained results are important for the promotion of use of L. fruticosa fruit extracts as a natural antioxidant in functional food production in the future.

scholarly journals A model experiment: competitive sorption of Cd, Cu, Pb and Zn by three different soils

2014 ◽  
Vol 9 (No. 3) ◽  
pp. 97-103 ◽  
Author(s):  
V. Zemanová ◽  
L. Trakal ◽  
P. Ochecová ◽  
J. Száková ◽  
D. Pavlíková
Keyword(s):  
Contaminated Soils ◽  
Model Experiment ◽  
Competitive Sorption ◽  
Metal Sorption ◽  
Clear Trend ◽  
Competitive Effects ◽  
Freundlich Isotherms ◽  
Uncontaminated Soil ◽  
Cu And Zn ◽  
Different Soils

The aim of this study was to evaluate and compare the competitive and individual sorption of Cd, Cu, Pb and Zn on three natural soils: a Gleyic Fluvisol (content of Cd 30, Cu 25, Pb 2297 and Zn 3718 mg/kg), a Gleyic Cambisol (content of Cd 5, Cu 29, Pb 1158 and Zn 180 mg/kg) and a Chernozem (content of Cd 0.4, Cu 36, Pb 75 and Zn 67 mg/kg). For evaluation of the sorption and desorption, the Freundlich isotherms were used. The results of the model experiment confirmed that the sorption from single-metal solution was more effective than sorption under multi-metal conditions, due to competitive effects. In all tested soils sorption of Cd, Cu and Zn decreased with the rate of other competitive metals; the Pb sorption was not affected by other competitive metals in solutions. Moreover, during multi-metal sorption, Zn was significantly desorbed in Cambisol. In general, sorptions of Cu, Pb and Zn were greater in uncontaminated soil compared to contaminated soils. Clear trend of impact of the contamination on Cd sorption was not observed. The results showed the sorption decreasing in order Chernozem &gt; Fluvisol &gt; Cambisol. The sorption was the greatest in uncontaminated soil with low mobility of studied metals.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

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