scholarly journals EFEITO DA ADMINISTRAÇÃO DO HORMÔNIO CORIÔNICO GONADOTRÓFICO (hCG) NA DURAÇÃO DO CICLO ESTRAL E NA TAXA DE PRENHEZ EM BOVINOS DE CORTE

2004 ◽  
Vol 9 (2) ◽  
Author(s):  
J.C. FANTINI FILHO ◽  
L.E. KOZICKI ◽  
F.P. SOUZA
Keyword(s):  
Estrous Cycle ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Cycle Length ◽  
Treated Group ◽  
Physiological Solution ◽  
Control Group ◽  
Control Groups ◽  
Intramuscular Route ◽  
And Control

No experimento foram utilizados 62 animais da raça Charolesa e Caracú, inseminados artificialmente (IA). No 7o dia após a IA, as vacas foram divididas aleatoriamente em 2 grupos: tratado (n=32), que recebeu 2.500 UI de hCG (IM) e controle (n=30), que recebeu 1 ml de solução fisiológica estéril, (IM). O diagnóstico de prenhez foi realizado por toque retal no 45o dia após a IA. Resultados: A duração do ciclo estral diferiu (P0,05) entre o grupo tratado e controle (40,62% e 23,33%, respectivamente). Conclusões: A duração do ciclo estral dos animais tratados foi prolongada em relação aos controles (P0,05) as taxas de prenhez em bovinos de corte. Effect of human chorionic gonadotropin (hCG) on estrous cycle length and pregnancy rates in cattle Abstract In the present experiment a total of 62 cows of Charoles and Caracú breeds, artificially inseminated (AI). At day 7th after AI the cows were aleatory distributed in two groups: treated group (n=32) that receive 2.500 UI of human chorionic gonadotropin (hCG) by the intramuscular route and control group (n=30) to wich 1.0 ml of sterile physiological solution was administered also by the intramuscular route. It has been found that the estrous cycle lenght was different (P0,05) between treated and the control groups (40,6 and 23,3%, respectively). In this way, the estrous cycle lenght of the treated cows was extended in regard to the control cows. The hCG administration did not increase pregnant rates.

scholarly journals Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

2009 ◽  
Vol 24 (5) ◽  
pp. 383-386 ◽  
Author(s):  
Cecília Maria de Carvalho Xavier Holanda ◽  
Monique Batista da Costa ◽  
Natália Chilinque Zambão da Silva ◽  
Maurício Ferreira da Silva Júnior ◽  
Vanessa Santos de Arruda Barbosa ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Aloe Vera ◽  
Treated Group ◽  
Control Group ◽  
Blood Levels ◽  
Control Groups ◽  
High Increase ◽  
Tropical Plant ◽  
And Control ◽  
The Brain

PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g) was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). CONCLUSION: The aqueous extract of Aloe vera facilitated the uptake of Na99mTcO4 in organs of rats and it was responsible to a high increase of levels of AST and ALT.

The effect of the laparoscopic insemination technique on the oestrous cycle of the ewe

1991 ◽  
Vol 62 (2) ◽  
pp. 60-61
Author(s):  
T. L. Taljaard ◽  
S. J. Terblanche ◽  
H. J. Bertschinger ◽  
L. J. Van Vuuren
Keyword(s):  
Cycle Length ◽  
Intrauterine Insemination ◽  
Uterine Horn ◽  
Oestrous Cycle ◽  
Control Group ◽  
Control Groups ◽  
The Mean ◽  
And Control ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Teaser Rams

This investigation was designed to determine whether or not the technique of intrauterine insemination affects the length of the subsequent oestrous cycle. Dorper ewes (n=31) were divided into treatment and control groups. All the ewes were synchronised using 40 mg fluorogestone acetate intravaginal sponges for 14 d and 300 IU pregnant mare serum gonadotrophin on the day of sponge removal. A standard semen diluent was deposited laparoscopically in each uterine horn of ewes in the treatment, group. Teaser rams were used to detect oestrus. Progesterone profiles were used to confirm oestrus. The mean oestrous cycle length of 17,83 ± 0,69 d for the group in which the diluent was deposited by laparoscopy did not differ significantly (P0,l) from the 18,36±2,11 d of the control group. The technique of laparoscopic insemination did not influence the length of subsequent oestrous cycles.

scholarly journals JAK2 inhibitor CEP-33779 prevents mouse oocyte maturation in vitro

2017 ◽  
Vol 37 (4) ◽  
Author(s):  
Changli Wu ◽  
Rong Li ◽  
Haibing Luo ◽  
Mingfeng Xu ◽  
Xiujuan Zhang
Keyword(s):  
Oocyte Maturation ◽  
Treated Group ◽  
Mouse Oocyte ◽  
Janus Kinase ◽  
Meiotic Spindle ◽  
Control Group ◽  
Control Groups ◽  
Germinal Vesicle Breakdown ◽  
Mouse Oocytes ◽  
And Control

The inhibitor CEP-33779 is a specific selective inhibitor of Janus kinase 2 (JAK2). In most somatic cells, JAK2 plays essential roles in cellular signal transduction and in the regulation of cell cycle. Little is known regarding the effects of JAK2 on mammalian oocyte maturation. In the present study, we investigated the effects of CEP-33779 on mouse oocytes’ meiosis and the possible mechanisms of JAK2 during mouse oocyte maturation. We detected the distribution of JAK2 during the mouse oocyte maturation. The results showed that JAK2 was mainly distributed in the cytoplasm during maturation. We cultured mouse oocytes with CEP-33779, examined the maturation rate, spindle morphology, and organization of microfilaments during the mouse oocyte maturation. While the rate of germinal vesicle breakdown (GVBD) did not differ between the treated and control groups, the rate of oocyte maturation decreased significantly when treated with CEP-33779. The rate of maturation was 21.14% in treated group and was 81.44% in control group. The results show that CEP-33779 inhibits the maturation of mouse oocytes. There was no obvious difference in the meiotic spindle morphology between the treated and control groups. The results show that CEP-33779 treatment did not disrupt the reorganization of microtubules. The microfilament observation shows that the microfilament did not form actin cap and the spindle stayed at the center of the oocyte in the treated group. CEP-33779 treatment inhibited the maturation of mouse oocytes which might be because of the disruption of formation of the actin cap. These results suggest that JAK2 regulated the microfilaments aggregation during the mouse oocyte maturation.

scholarly journals Adding Midostaurin to Intensive Chemotherapy in FLT3-Positive AML Patients. Results from a Multicenter Historical-Controlled Study

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 4005-4005 ◽  
Author(s):  
Tamar Berger ◽  
Yakir Moshe ◽  
Shilo Yaari ◽  
Ilana Hellmann ◽  
Adina Aviram ◽  
...  
Keyword(s):  
Overall Survival ◽  
Cox Regression ◽  
Treated Group ◽  
Historical Control ◽  
Phase Iii ◽  
Control Group ◽  
Control Cohort ◽  
Control Groups ◽  
And Control

Abstract Introduction: The phase III RATIFY study (Stone et al, NEJM 2017) demonstrated that the addition of the FLT3 inhibitor midostaurin to intensive induction and consolidation courses improves outcome in younger FLT3-positive AML patients. The toxicity and efficacy profile of adding midostaurin to chemotherapy in patients not originally included in the RATIFY study is unknown. We sought to characterize midostaurin use in a 'real-world' setting. Methods: Patients (>18 years) with FLT3-positive AML (ITD/TKD) were eligible to receive midostaurin through the Novartis extended access program that was launched in Israel in April 2016. In order to control for toxicity and efficacy outcomes in the midostaurin-treated patients, a historical control cohort was created that included patients with FLT3-positive AML from 2 participating centers that were not treated with midostaurin (all patients diagnosed after January 2015). Data were extracted from electronic patient records and were collected from several medical centers in Israel. Base-line characteristics, disease- and patient- specific parameters as well as relapse-rates and overall survival were analyzed and compared between the midostaurin treated and untreated cohorts. We used Cox regression to analyze predictors for survival. This study was approved by the Institutional Review Board. Results: Thirty-five patients were included in the analysis. The median age of the patients was 62 years (range 27-78); 40% and 20% of patients were over the age of 65 and 70 years, respectively. FLT3-ITD mutations were detected in 32 patients (91%) and 3 patients had TKD mutations (9%). Eight patients (23%) had secondary leukemia, 83% had normal karyotype and 57% were NPM1-mutated. No differences were noted between the midostaurin-treated group (n=21) and the historical control cohort (n=14) in terms of age, gender, leukemia ontogeny, cytogenetics, presenting blood counts, extramedullary involvement, performance status and comorbidity scales. More patients in the midostaurin group were found to be NPM1-mutated (66 vs. 44%, p=0.03). Furthermore, no differences were noted between the groups in terms of daunorubicin dose for induction (45, 60 and 90 mg/m2/dayin 20, 30 and 50% of patients, respectively), number of consolidations (median number of cycles - 2), cytarabine dose or allogeneic transplantation rate (45 and 36% in the midostaurin and control group, respectively). The full 14 day midostaurin course was given in most patients during induction (73%). In 5 patients midostaurin was initiated only at the post-induction courses due to technical delays in drug supply. Only 4 patients experienced dose reductions or interruptions during therapy: 3 during induction (septic shock, drug interaction and QT prolongation) and 1 during consolidation (new onset atrial fibrillation). Toxicity was comparable between the cohorts. Febrile neutropenia during induction was noted in 95 and 93% of patients in the midostaurin and control groups, respectively. Time to neutrophil and platelet recovery were also comparable (25 vs. 24 days and 24 vs. 20 days in the midostaurin and control groups respectively). Other toxicities were uncommon and not significantly different between the groups. CR/CRi rates were 85% and 58% in the midostaurin and control cohorts, respectively (p=0.17). The median follow-up time for surviving patients in the midostaurin and control cohorts were 426 and 517 days, respectively (p=0.55). During follow-up, 7 deaths occurred in the midostaurin group and 9 in the control arm. Median survival was not reached for the midostaurin treated group and was 281 days for the control group (Figure 1, p=0.42). Nine and 6 patients in remission relapsed in the midostaurin and control group, respectively, translating into a relapse-rate of 53% and 86%, respectively (p=0.19). No difference in early death rate was noted between the groups. The only factor that significantly affected overall survival in the COX-regression analysis was white blood cell count at diagnosis (p=0.03). Conclusions: In the off-trial setting, midostaurin is administered across all age groups and various FLT3-positive subtypes. In this 'real-life' setting, midostaurin is well tolerated and does not significantly add to the toxicity of chemotherapy. Longer follow-up and more patients are needed to assess the efficacy of adding midostaurin to chemotherapy in this group of patients. Figure 1. Figure 1. Disclosures Ofran: Novartis: Other: Served on a Novartis advisory board.

444 FOLLICLE ASPIRATION/ABLATION AS A TECHNIQUE TO AVOID OVULATION AND INDUCE DIESTRUS CONCENTRATIONS OF PROGESTERONE DURING THE ESTROUS CYCLE IN MARES

2010 ◽  
Vol 22 (1) ◽  
pp. 379
Author(s):  
F. S. Ignacio ◽  
D. F. Montechiesi ◽  
D. R. Bergfelt ◽  
J. N. P. Puoli Filho ◽  
L. R. Carvalho ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Embryo Transfer ◽  
Follicular Development ◽  
Control Group ◽  
Control Groups ◽  
Plasma Progesterone ◽  
Luteal Regression ◽  
Follicle Aspiration ◽  
Mean Differences ◽  
And Control

The present study aimed to demonstrate that ultrasound-guided follicle aspiration/ablation could circumvent ovulation and induce progesterone production in potential recipient mares sufficient to reach diestrus concentrations and potentially support pregnancy following embryo transfer. Ovulation (Day 0) was detected and follicular development was monitored using transrectal ultrasonography in 29 horse mares during the estrous cycle. On Day 5, all mares were given PGF2α (7.5 mg of Dinoprost trometamine, Lutalyse®, Pfizer, Paulinia, Brazil) and randomly assigned to a control and follicle ablation groups. Mares in the control group (n = 5) were not subject to follicle ablation and were allowed to ovulate. Mares in the ablation groups were distributed according to the diameter of the largest follicle to be ablatedusing transvaginal ultrasonography as follows: F25-29 mm (n = 6); F30-34 mm (n = 6); F35-40 mm (n = 6); F ≥ 40 (n = 6). Blood samples were collected at 24-h intervals from the day before ovulation or follicle ablation until detection of the next ovulation. Plasma progesterone concentrations were measured using a commercial RIA kit with intra- and interassay CV of 7.6% and 8.3%, respectively. Concentrations of progesterone ≥2 ng mL-1 were considered representative of diestrus in the ablation groups. Groups were compared using ANOVA and mean differences among groups were located with Tukey’s test. There was an effect of group (P < 0.05) and the percentage of mares and mean (± SEM) days post-ablation or ovulation that progesterone was ≥2 ng mL-1 for each follicle group were as follows: F25-29, 83% and 6.2 ± 1.1ab; F30-34, 66% and 7.3 ± 0.5a; F35-40, 83% and 5.4 ± 0.6ab; F ≥ 40, 100% and 4.0 ± 0.4bc; and control, 100% and 1.6 ± 0.4c. Values with different superscripts are different (P < 0.05). There was no difference (P > 0.05) in maximum concentrations of progesterone among ablation groups. For 7 days after follicle ablation, concentrations of progesterone (≥2 ng mL-1) were not different (P > 0.05) between ablated and control groups. Correspondingly, the time to spontaneous luteal regression (i.e. progesterone <2 ng mL-1) post-ablation or ovulation was not different (P > 0.05) among groups. In conclusion, aspiration/ablation of follicles ≥25 mm following luteal regression during the estrous cycle resulted in the production of progesterone such that concentrations attained diestrus values that could potentially sustain pregnancy following embryo transfer. The authors wish to acknowledge FUNDUNESP for funding the project.

192 The use of human chorionic gonadotropin seven days after synchronized estrus for the increase of luteal tissue in Morada Nova ewes

2019 ◽  
Vol 31 (1) ◽  
pp. 220
Author(s):  
G. B. Vergani ◽  
J. T. Trevizan ◽  
V. S. A. Pereira ◽  
J. F. Fonseca ◽  
A. R. Garcia ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Physiological Solution ◽  
Saline Control ◽  
Favourable Effect ◽  
Control Group ◽  
Corpora Lutea ◽  
Control Groups ◽  
Luteal Tissue ◽  
Condition Score ◽  
And Control

The study was performed to evaluate whether the use of hCG 7 days after synchronized oestrus in Morada Nova ewes would increase the number and size of corpora lutea. Multiparous Morada Nova ewes (n=115, mean weight of 36.6kg, body condition score 3.13 on 1 to 5 scale) were used. Oestrus was synchronized by using an intravaginal sponge impregnated with medroxyprogesterone acetate (60mg, Progespon®, Zoetis, Parsippany, NJ, USA) for 6 days and eCG (200IU, IM Novormon®, Zoetis) plus PGF2α analogue (37.5μg, D-cloprostenol, IM, Vetglan®, Hertape Calier, Spain), both administered 24h before the sponge removal. Seven days after the synchronized oestrus, hCG (300IU, IM, Vetecor®, Hertape Calier; n=57) or normal saline (control group, 1mL, IM, 0.9% NaCl, Eurofarma Laboratory SA, Sao Paulo, Brazil; n=58) was injected. B-mode ultrasound examinations of the ovaries were performed using the equipment MyLab Vet 30 Gold (Esoate, Genoa, Italy) on Day 7 (corresponding to the day of hCG or physiological solution administrations), and 6 days later (Day 13). The number of corpora lutea present and the size of luteal tissue (sum of diameter and area) were determined. Data were analysed by ANOVA with Tukey’s post hoc test (mean±s.e.m.) using SAS software (SAS Institute Inc., Cary, NC, USA). On Day 7, the number of corpora lutea was similar (P&gt;0.05) between the hCG and control groups (1.58±0.09v. 1.57±0.08, respectively); however, the number was higher on Day 13 (P&lt;0.05) in the hCG group (2.65±0.13) than in the control group (1.69±0.07). Similarly, there was no difference (P&gt;0.05) between groups in diameter (16.9±0.82v. 16.0±0.71mm) and area (1.61±0.07v. 1.45±0.06cm2) for hCG and control groups, respectively, on Day 7. Differences were observed on Day 13, such that the hCG ewes had luteal tissue of larger (P&lt;0.0001) diameters (27.13±1.25v. 18.44±0.85mm) and area (2.81±0.13v. 1.71±0.08cm2) than ewes of the control group. In conclusion, 300IU of hCG injected 7 days after synchronized oestrus is effective to increase the number of corpora lutea and in the size of luteal tissue. Despite indications of a favourable effect on luteal dynamics, the effects on progesterone production and conception rate need to be examined. This study was supported by CNPq and EMBRAPA (process no. 02.13.06.026.00.02).

The Acupuncture Treatment of Neck Pain: A Randomized Controlled Study

1981 ◽  
Vol 09 (04) ◽  
pp. 326-332 ◽  
Author(s):  
Ralph M. Coan ◽  
Grace Wong ◽  
Patricia L. Coan
Keyword(s):  
Pain Score ◽  
Treated Group ◽  
Equal Treatment ◽  
Controlled Study ◽  
Control Group ◽  
Control Groups ◽  
Pain Syndromes ◽  
Spine Pain ◽  
Reduction Of Pain ◽  
And Control

Thirty patients with cervical spine pain syndromes persisting a mean of 8 years were assigned randomly into equal treatment and control groups. After 12 weeks, 12 of 15 (80%) of the treated group felt improved, some dramatically, with a mean 40% reduction of pain score, 54% reduction of pain pills, 68% reduction of pain hours per day and 32% less limitation of activity. Two of 15 (13%) of the control group reported slight improvement after 12.8 weeks. The control group had a mean 2% worsening of the pain score, 10% reduction in pain pills, no lessening of pain hours and 12% less limitation of activity.

scholarly journals Comparative studies of early season moxidectin treatment and conventional ivermectin/ benzimidazole treatments in the control of cyathostomes in horses

2012 ◽  
Vol 48 (No. 1 - 2) ◽  
pp. 9-17
Author(s):  
I. Langrová ◽  
I. Jankovská ◽  
M. Borovský
Keyword(s):  
Live Weight ◽  
Treated Group ◽  
Control Group ◽  
Control Groups ◽  
Grazing Period ◽  
The Mean ◽  
Faecal Egg Counts ◽  
Pre Treatment ◽  
And Control ◽  
First Time

Moxidectin administered in January or February at a single dose was tested for efficacy in horses on two farms for 12 and 11 months, respectively. Horses were infected with cyathostomes naturally in the previous grazing period. Forty horses of farm 1 and 20 horses of farm 2 were used in controlled tests to evaluate the efficacy of moxidectin 2% gel formulation at the dosage 0.4 mg moxidectin per kg of live weight, ivermectin commercial paste formulation at the dosage 0.2 mg ivermectin per kg of live weight, mebendazole and fenbendazole commercial paste formulation at the dosage both 7.5 mg mebendazole and fenbendazole per kg of live weight, all applied orally. Three control groups of 10 horses each (farm 1) were treated twice a year with ivermectin and benzimidazoles, respectively. Individual faecal egg counts, faecal cultures and larval differentiation were performed. Moxidectin had more prolonged and greater suppressive effects on the post-treatment reappearance and magnitude of strongyle egg counts than did ivermectin or benzimidazoles. In the moxidectin treated group (M1) strongyle eggs were seen for the first time in April and a slight increase in the mean count of eggs per gram of faeces (EPG) was observed during the rest of the season. Litter larval counts significantly reflected levels of exposure during the tested season. Twenty animals of farm 2 were allocated into two groups of ten horses each based on pre-treatment eggs per gram (EPG) counts (moxidectin treated group and control group). In the moxidectin treated group mean egg counts remained very low throughout the study. A plateau was reached by autumn, with egg counts ranging from 74 to 145 EPG. The faecal egg counts of moxidectin treated group (M2) were significantly higher in March, April, May and June.

scholarly journals Effect of Adiponectin Hormone on Serum Lipoproteins Levels in Female Rats

2013 ◽  
Vol 12 (2) ◽  
pp. 50
Author(s):  
H, A. A. Al-Baka`a
Keyword(s):  
Normal Saline ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Serum Lipoproteins ◽  
Control Groups ◽  
Dose Administration ◽  
Blood Samples ◽  
And Control

This investigation aimed to study the effect of adiponectin hormone on serum lipoproteins levels in female rats. For this purpose, thirteen female rats were divided into treated and control groups. Each animal in the treated group received a daily intraperitoneal 25microgram does of adiponectin dissolved in 0.2ml normal saline for fifteen respective days. In the same time, each animal in the control group received a daily intraperitoneal 0.2ml normal saline for fifteen respective days. The animal has fasted for 12 hours after last dose administration. Blood samples were collected, and the sera obtained. Analysis showed a significant increase in the serum HDL level and a significant decrease in LDL concentration. AP < 0.05was considered significant.

Biotreatment of brewery effluents for aquaculture use using autochthonous fungi

2021 ◽  
Vol 29 (2) ◽  
pp. 123-134
Author(s):  
J.P Udoh ◽  
A.J Otoh ◽  
M.E Udang
Keyword(s):  
Treated Group ◽  
Control Group ◽  
Environmental Standards ◽  
Control Groups ◽  
Dissolved Inorganic Nutrients ◽  
Standards And Regulations ◽  
And Control ◽  
National Environmental ◽  
Potential Use ◽  
Brewery Effluent

The potential of reducing environmental impact of untreated brewery effluent was investigated. Although concentrations of pollutants in such effluents are usually considered low and inadvertently discharged into adjacent urban drainage facility, mycoremediation to remove dissolved inorganic nutrients in effluent was conducted with a view for aquaculture use in Uyo metropolis, southeast Nigeria. Raw brewery effluents were obtained and screened for indigenous microbial flora. Autochthonous fungi isolated included Aspergillus niger, Verticillium sp. and Mucor sp. The potential use of isolates as alternative treatment of brewery effluents was analyzed for treated and control groups. Treated group was inoculated with pure colonies of fungi isolates while the control group received no fungi treatment or modification. Both groups were incubated for seven (7) days. Thereafter, the physicochemical parameters of raw and remediated effluents were analysed and compared with National Environmental Standards and Regulations Enforcement Agency (NESREA) and aquaculture standards. Results confirmed suitability of autochthonous fungi isolates in mycoremediation of brewery effluent for aquaculture and irrigation. Key words: effluent toxicity, industrial pollution, mycoremediation, aquatic ecosystem, Uyo

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