THYME OIL LOADED CASSAVA STARCH TRANSDERMAL FILM FOR WOUND HEALING

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (02) ◽  
pp. 76-81
Author(s):  
Vinita Patole ◽  
Rajnigandha Gaikwad ◽  
Kishanchandra Khandelwal ◽  
Keyword(s):  
Wound Healing ◽  
Chorioallantoic Membrane ◽  
Antimicrobial Properties ◽  
Volatile Oil ◽  
Cassava Starch ◽  
Vapour Permeability ◽  
Angiogenic Potential ◽  
Thyme Oil ◽  
Chick Chorioallantoic Membrane ◽  
Transdermal Film

Biodegradable cassava starch-based transdermal film loaded with thyme volatile oil was prepared by solvent casting method for wound healing activity. The stretchability, water vapour permeability and antimicrobial properties of the film were measured. The films were evaluated for drug-excipient compatibility studies by Fourier transformed infrared spectroscopy (FTIR). The formulated film loaded with thyme volatile oil exhibited good anti-microbial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The film was tested to determine its potential to increase the number of capillaries on the treated chick chorioallantoic membrane (CAM) surfaces using nine days old fertilized chick eggs. These thyme oil films loaded with cassava starch displayed angiogenic potential, which is required in the treatment of wound healing.

scholarly journals In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Elizabeth A. Wahl ◽  
Fernando A. Fierro ◽  
Thomas R. Peavy ◽  
Ursula Hopfner ◽  
Julian F. Dye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Chorioallantoic Membrane ◽  
Angiogenic Potential ◽  
Chick Chorioallantoic Membrane ◽  
Bovine Collagen

Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis.In vitroresults demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCsin vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.

scholarly journals Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3284
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Zulfiqar Ahmad Rehan ◽  
Hamna Tayyab ◽  
Talha Javed ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Chorioallantoic Membrane ◽  
Calotropis Procera ◽  
Connective Tissues ◽  
Blood Capillaries ◽  
Chick Chorioallantoic Membrane ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

scholarly journals Investigation of Angiogenesis and Wound Healing Potential Mechanisms of Zinc Oxide Nanorods

2021 ◽  
Vol 12 ◽  
Author(s):  
Amr Hassan ◽  
Ahmed I. Abd El Maksoud ◽  
Dalia Elebeedy ◽  
Mohammad A. Zoair ◽  
Emadeldin R. Matar
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Zno Nanorods ◽  
Chorioallantoic Membrane ◽  
Zinc Oxide Nanorods ◽  
Vegf Expression ◽  
Angiogenic Potential ◽  
Oxide Nanorods ◽  
Angiogenesis Process ◽  
Chorioallantoic Membrane Assay

The angiogenesis process is an essential issue in tissue engineering. Zinc oxide nanorods are biocompatible metals capable of generating reactive oxygen species (ROS) that respond to induced angiogenesis through various mechanisms; however, released Zn (II) ions suppress the angiogenesis process. In this study, we fabricated green ZnO nanorods using albumin eggshell as a bio-template and investigate its angiogenic potential through chorioallantoic membrane assay and excision wound healing assay. This study demonstrated that angiogenesis and wound healing processes depend on pro-angiogenic factors as VEGF expression due to ZnO nanorods' exiting. Angiogenesis induced via zinc oxide nanorods may develop sophisticated materials to apply in the wound healing field.

scholarly journals In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications

2020 ◽  
Vol 21 (5) ◽  
pp. 1633 ◽  
Author(s):  
Fatma Z. Kocak ◽  
Abdullah C.S. Talari ◽  
Muhammad Yar ◽  
Ihtesham U. Rehman
Keyword(s):  
Bone Regeneration ◽  
Sol Gel ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Injectable Hydrogels ◽  
Angiogenic Potential ◽  
Homogeneous Solutions ◽  
Chick Chorioallantoic Membrane ◽  
Thermosensitive Hydrogels

Biomaterials that promote angiogenesis are required for repair and regeneration of bone. In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration. In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential. Hydrogel formulations with varying Hep concentrations were prepared by sol–gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5–10 minutes. Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses. Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections. Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels. Hydrogels induced microvascularity in CAM assay this was confirmed by histology analyses. Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response. Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.

scholarly journals Towards the Development of a Novel Ex Ovo Model of Infection to Pre-Screen Biomaterials Intended for Treating Chronic Wounds

2020 ◽  
Vol 11 (2) ◽  
pp. 37 ◽  
Author(s):  
Elena García-Gareta ◽  
Justyna Binkowska ◽  
Nupur Kohli ◽  
Vaibhav Sharma
Keyword(s):  
Staphylococcus Aureus ◽  
Persistent Infection ◽  
Scientific Community ◽  
Chronic Wounds ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Antimicrobial Properties ◽  
Ex Vivo Model ◽  
Angiogenic Potential ◽  
Chick Embryo Chorioallantoic Membrane

This communication reports preliminary data towards the development of a live ex vivo model of persistent infection that is based on the chick embryo chorioallantoic membrane (CAM), which can be used for pre-screening biomaterials with antimicrobial properties for their antimicrobial and angiogenic potential. Our results showed that it was possible to infect chicken embryos with Staphylococcus aureus, one of the main types of bacteria found in the persistent infection associated with chronic wounds, and maintain the embryos’ survival for up to 48 h. Survival of the embryos varied with the dose of bacteria inoculum and with the use and time of streptomycin application after infection. In infected yet viable embryos, the blood vessels network of the CAM was maintained with minimal disruption. Microbiological tests could confirm embryo infection, but quantification was difficult. By publishing these preliminary results, we hope that not only our group but others within the scientific community further this research towards the establishment of biomimetic and reproducible ex vivo models of persistent infection.

scholarly journals Development of Hydrogels with the Incorporation of Raphanus sativus L. Seed Extract in Sodium Alginate for Wound-Healing Application

Gels ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 107
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Ayesha Afzal ◽  
Zulfiqar Ahmad Rehan ◽  
Muzzamil Mehmood ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sodium Alginate ◽  
Raphanus Sativus ◽  
Research Work ◽  
Chorioallantoic Membrane ◽  
Seed Extract ◽  
Cutaneous Lesions ◽  
Chick Chorioallantoic Membrane ◽  
Raphanus Sativus L ◽  
Seed Extracts

Hydrogels prepared from polymers have been proposed for tissue regeneration and the treatment of bruise wounds. In this research work, we synthesized a Raphanus sativus L.-based wound-healing hydrogel with recognized antimicrobial activity for the healing of cutaneous lesions, drawing on its healing potential. A structural analysis was performed by Fourier transform infrared spectroscopy, confirming the interaction between sodium alginate and Raphanus sativus L. The surface morphology was studied by scanning electron microscopy. A swelling test showed that the T-1 hydrogel capability of absorption of the solution was superior compared to other synthesized samples. It was evident that the swelling tendency decreased as the Raphanus sativus L. seed extract concentration was reduced. In a thermogravimetric analysis, T-1 shows high thermal stability over other prepared hydrogel samples, enjoying a high content of seed extract compared with all samples. The prepared hydrogels were placed on the chick chorioallantoic membrane of fertilized chick eggs, and their healing capability was examined. All seed extracts containing hydrogels showed clear curative performance as compared to the control hydrogel, whereas their healing magnitude lessened as the extract ratio decreased. It was concluded from the results of the current study that the Raphanus sativus L. plant has wound-healing characteristics.

scholarly journals Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 590
Author(s):  
Lucy Vojtová ◽  
Veronika Pavliňáková ◽  
Johana Muchová ◽  
Katarína Kacvinská ◽  
Jana Brtníková ◽  
...  
Keyword(s):  
Wound Healing ◽  
Comprehensive Evaluation ◽  
Chorioallantoic Membrane ◽  
Histological Evaluation ◽  
Skin Defect ◽  
Angiogenic Potential ◽  
Freeze Dried ◽  
Chitosan Scaffolds

Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 °C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB® were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB® already at very low concentrations (0.01 µg/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.

scholarly journals Neuroprotection in early stages of Alzheimer’s disease is promoted by transthyretin angiogenic properties

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Tiago Gião ◽  
Joana Saavedra ◽  
José Ricardo Vieira ◽  
Marta Teixeira Pinto ◽  
Gemma Arsequell ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Chorioallantoic Membrane ◽  
Tube Formation ◽  
Cam Assay ◽  
Vessel Length ◽  
Angiogenic Potential ◽  
Chick Chorioallantoic Membrane ◽  
Brain Microvessels

Abstract Background While still controversial, it has been demonstrated that vascular defects can precede the onset of other AD hallmarks features, making it an important therapeutic target. Given that the protein transthyretin (TTR) has been established as neuroprotective in AD, here we investigated the influence of TTR in the vasculature. Methods We evaluated the thickness of the basement membrane and the length of brain microvessels, by immunohistochemistry, in AβPPswe/PS1A246E (AD) transgenic mice and non-transgenic mice (NT) bearing one (TTR+/−) or two (TTR+/+) copies of the TTR gene. The angiogenic potential of TTR was evaluated in vitro using the tube formation assay, and in vivo using the chick chorioallantoic membrane (CAM) assay. Results AD transgenic mice with TTR genetic reduction, AD/TTR+/−, exhibited a thicker BM in brain microvessels and decreased vessel length than animals with normal TTR levels, AD/TTR+/+. Further in vivo investigation, using the CAM assay, revealed that TTR is a pro-angiogenic molecule, and the neovessels formed are functional. Also, TTR increased the expression of key angiogenic molecules such as proteins interleukins 6 and 8, angiopoietin 2, and vascular endothelial growth factor, by endothelial cells, in vitro, under tube formation conditions. We showed that while TTR reduction also leads to a thicker BM in NT mice, this effect is more pronounced in AD mice than in NT animals, strengthening the idea that TTR is a neuroprotective protein. We also studied the effect of TTR tetrameric stabilization on BM thickness, showing that AD mice treated with the TTR tetrameric stabilizer iododiflunisal (IDIF) displayed a significant reduction of BM thickness and increased vessel length, when compared to non-treated littermates. Conclusion Our in vivo results demonstrate the involvement of TTR in angiogenesis, particularly as a modulator of vascular alterations occurring in AD. Since TTR is decreased early in AD, its tetrameric stabilization can represent a therapeutic avenue for the early treatment of AD through the maintenance of the vascular structure.

scholarly journals Development and Evaluation of Rifampicin Loaded Alginate–Gelatin Biocomposite Microfibers

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1514
Author(s):  
Ameya Sharma ◽  
Vivek Puri ◽  
Pradeep Kumar ◽  
Inderbir Singh ◽  
Kampanart Huanbutta
Keyword(s):  
Wound Healing ◽  
Xanthan Gum ◽  
In Vitro Release ◽  
Antimicrobial Properties ◽  
Entrapment Efficiency ◽  
Tissue Proliferation ◽  
Naturally Occurring ◽  
Reinforcing Material ◽  
Vitro Release

Various systematic phases such as inflammation, tissue proliferation, and phases of remodeling characterize the process of wound healing. The natural matrix system is suggested to maintain and escalate these phases, and for that, microfibers were fabricated employing naturally occurring polymers (biopolymers) such as sodium alginate, gelatin and xanthan gum, and reinforcing material such as nanoclay was selected. The fabrication of fibers was executed with the aid of extrusion-gelation method. Rifampicin, an antibiotic, has been incorporated into a biopolymeric solution. RF1, RF2, RF3, RF4 and RF5 were coded as various formulation batches of microfibers. The microfibers were further characterized by different techniques such as SEM, DSC, XRD, and FTIR. Mechanical properties and physical evaluations such as entrapment efficiency, water uptake and in vitro release were also carried out to explain the comparative understanding of the formulation developed. The antimicrobial activity and whole blood clotting of fabricated fibers were additionally executed, hence they showed significant results, having excellent antimicrobial properties; they could be prominent carriers for wound healing applications.

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