ASSESSMENT OF PERMEABILITY BEHAVIOR OF BERBERINE CHLORIDE ACROSS GOAT INTESTINAL MEMBRANE IN PRESENCE OF NATURAL BIOPOTENTIATOR CURCUMIN

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (4) ◽  
pp. 23-27
Author(s):  
Sarika Narade ◽  
Yogesh Pore ◽  
◽  
Keyword(s):  
Efflux Pump ◽  
Diffusion Cell ◽  
Apparent Permeability ◽  
Franz Diffusion Cell ◽  
Permeability Characteristics ◽  
Berberine Chloride ◽  
Intestinal Membrane ◽  
Permeability Studies ◽  
Cell Data ◽  
Intestinal Efflux

The present study investigates the influence of co-administration of different concentrations (2, 6, and 10 mg) of curcumin on goat intestinal permeability of berberine chloride (BBC) using Franz diffusion cell. Data obtained in triplicate from permeability studies were used to calculate percentage cumulative drug release (% CDR), apparent permeability (Papp), flux (J) and enhancement ratio (ER). Co-administration of 6 mg concentration of curcumin with BBC was found to be optimum to enhance the permeability of BBC up to 23.92 ± 0.78 % CDR, over control (8.49 ± 1.45 % CDR). At the optimized concentration of curcumin, permeability characteristics were improved significantly compared to control. The present study reveals the beneficial effect of co-administration of curcumin (6 mg) to promote membrane permeability of BBC which would be expected to improve its bioavailability, thereby therapeutic efficacy. The effect could be attributed to curcumin-mediated inhibition of intestinal efflux pump P-gp, acting as an absorption barrier for BBC.

scholarly journals Effect ofIn VitroTranscorneal Approach of Aceclofenac Eye Drops through Excised Goat, Sheep, and Buffalo Corneas

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Vivek Dave ◽  
Sarvesh Paliwal ◽  
Sachdev Yadav ◽  
Swapnil Sharma
Keyword(s):  
Ophthalmic Solution ◽  
Linear Increase ◽  
Eye Drops ◽  
Diffusion Cell ◽  
Solution Ph ◽  
Apparent Permeability ◽  
Franz Diffusion Cell ◽  
Apparent Permeability Coefficient ◽  
Ophthalmic Formulation

The current study involves the evaluation of factors that influence the transcorneal permeation of aqueous drops of aceclofenac ophthalmic formulation through freshly excised goat, sheep, and buffalo corneas. Aceclofenac formulation with different concentrations 0.1–0.5% (w/v) and with different pH and different preservatives, was taken into account. The amount of drug permeated from different formulations was estimated using an Franz diffusion cell. A linear increase in drug permeation was observed with increase in pH (5.5 to 7.4). The apparent permeability coefficient was found to be maximum15.01±0.45on goat cornea and maximum transport of aceclofenac was observed at physiological pH of tears (i.e., 7). The results advocate that aceclofenac 0.5% (w/v) ophthalmic solution (pH 7.0) containing BAK (0.01%) provides maximumin vitroocular permeability through goat, sheep, and buffalo corneas.

scholarly journals Permeability studies of Damar Batu free films for transdermal application

2009 ◽  
Vol 15 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Atish Mundada ◽  
Jasmine Avari
Keyword(s):  
Tensile Strength ◽  
Moisture Absorption ◽  
Chloroform Solution ◽  
Polyethylene Glycol 400 ◽  
Permeability Characteristics ◽  
Zero Order Kinetics ◽  
Transdermal Application ◽  
Model Drug ◽  
Free Films ◽  
Permeability Studies

Damar Batu (DB) looks like stone with black or dark brown color inside. Due to its film forming ability it is used in the manufacture of paper, wood, varnishes, lacquers, polishes and additives for beverages. In the present study the permeability of free films of DB casted from chloroform solution containing different plasticizers was studied with a view to developing a suitable rate controlling membrane for transdermal use. The free films of DB were prepared by a mercury substrate technique and dibutyl sebacate (DBS), dibutyl phthalate (DBP), polyethylene glycol 400 (PEG400) and propylene glycol (PG) were tried as plasticizers. DB films were then evaluated for uniformity of thickness, moisture absorption, water vapour transmission, tensile strength, percentage elongation and folding endurance. Permeability characteristics of free films of DB were studied using diltiazem hydrochloride (DH) as a model drug. Little variation in film thickness ensured the uniformity of the films. DBS produces tough DB films with more tensile strength. Drug diffusion through the free films followed zero order kinetics. The films plasticized with PEG400 showed higher permeability for DH compared with other films. The order of decrease of permeability of plasticized films with plasticizers was PEG400 > PG > DBP > DBS. Diffusion of drugs through the free films of DB was extended over a longer period of time at a controlled rate. DB seems to be a promising rate controlling membrane for the transdermal application.

Comparative Effects of Span 20 and Span 40 on Liposomes Release Properties

2017 ◽  
Vol 13 (12) ◽  
Author(s):  
Radoslava N. Pravilović ◽  
Bojana D. Balanč ◽  
Kata T. Trifković ◽  
Verica B. Đorđević ◽  
Nevenka M. Bošković-Vragolović ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Natural Antioxidants ◽  
Polyphenolic Compounds ◽  
Diffusion Cell ◽  
Transfer Resistance ◽  
Franz Diffusion Cell ◽  
Model Substance ◽  
Different Types ◽  
Liposome Size ◽  
The Common

AbstractLiposomes are known as convenient carriers for a broad range of actives. The release rate of actives can be adjusted/controlled by the addition of different surfactants. The aim of this study was to compare the effects of two surfactants, Span 20 and Span 40, on liposomes release properties. In particular, the study shows how the membrane modification affects the mass transfer resistances and liposome size compared with the common liposomes. The aqueous extract of thyme was used as a model substance. It is a valuable source of natural antioxidants – polyphenols. The diffusion of polyphenols from native thyme extract (un-encapsulated) and from different types of liposomes was conducted using Franz diffusion cell. The results were approximated with Fick’s second law and they implied that liposomes modified with Span 20 provided the slowest release of polyphenolic compounds due to the highest value of mass transfer resistance (2.523 × 106s/m).

scholarly journals On-line automated evaluation of lipid nanoparticles transdermal permeation using Franz diffusion cell and low-pressure chromatography

Talanta ◽  
2016 ◽  
Vol 146 ◽  
pp. 369-374 ◽  
Author(s):  
Ana Catarina Alves ◽  
Inês I. Ramos ◽  
Cláudia Nunes ◽  
Luís M. Magalhães ◽  
Hana Sklenářová ◽  
...  
Keyword(s):  
Low Pressure ◽  
Lipid Nanoparticles ◽  
Diffusion Cell ◽  
Franz Diffusion Cell ◽  
Transdermal Permeation ◽  
Automated Evaluation ◽  
On Line

scholarly journals The influence of membrane composition on the release of polyphenols from liposomes

2015 ◽  
Vol 69 (4) ◽  
pp. 347-353 ◽  
Author(s):  
Radoslava Pravilovic ◽  
Vesna Radunovic ◽  
Nevenka Boskovic-Vragolovic ◽  
Branko Bugarski ◽  
Rada Pjanovic
Keyword(s):  
Tween 20 ◽  
Diffusion Resistance ◽  
Diffusion Cell ◽  
Membrane Composition ◽  
Membrane Modification ◽  
Liposome Membrane ◽  
Franz Diffusion Cell ◽  
Therapeutic Properties ◽  
Tween 60 ◽  
Tea Extract

Polyphenols are compound which are widely studied because of their antioxidative and potential therapeutic properties. Systems for the controlled release of drugs offer a number of benefits comparing with traditional forms of medicines and because of that these systems are widely researched. The objective of this paper is to investigate the possibility of using liposomes as carriers of polyphenols and influence the membrane composition on the release rate of encapsulated polyphenols. Experiments show how the membrane modification affects the mass transfer comparing to a conventional liposomes. Liposomes were modified with surfactants Tween 20 and Tween 60, and thyme tea extract was used as a source of polyphenols. The diffusion of polyphenols from thyme extract, dispersion of conventional liposomes and liposomes modified with Tween 20 and Tween 60 was studied using Franz diffusion cell. From the experimental data diffusion coefficients were determined for each of the systems, as well as the corresponding diffusion resistances. From the obtained results it can be concluded that the encapsulation of polyphenols in liposomes significantly slows diffusion, and with membrane modification can be achieved further slowing. The diffusion resistance of the liposome membrane modified with Tween 20 and Tween 60 is about 5 times higher compared to the diffusion of unencapsulated polyphenols from the thyme extract.

scholarly journals Evaluation on Intestinal Permeability of Phlorotannins Using Caco-2 Cell Monolayers

2022 ◽  
Vol 17 (1) ◽  
pp. 1934578X2110704
Author(s):  
Naoki Murata ◽  
Saki Keitoku ◽  
Hideo Miyake ◽  
Reiji Tanaka ◽  
Toshiyuki Shibata
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
The Other ◽  
Intestinal Epithelial ◽  
Liquid Chromatography Mass Spectrometry ◽  
Apparent Permeability ◽  
Cell Monolayers ◽  
Intestinal Membrane ◽  
Small Intestinal ◽  
Conjugated Metabolites

Among the phlorotannins of seaweed polyphenols, eckols which have a dibenzodioxin linkage are known to have various physiological functions. The purpose of this study was to investigate the intestinal epithelial absorption of eckols using Caco-2 cell monolayers of the small intestinal membrane model. Each compound permeated from the apical (AP) side to the basolateral (BL) side in the monolayers was identified and quantitated by liquid chromatography-mass spectrometry with electrospray ionization. In the transport assays using five types of eckols (eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, and 8,8'-bieckol), only the monomeric eckol showed limited transepithelial absorption with relatively small apparent permeability values (0.30  ±  0.04  ×  10−8 cm/s). Analyzing the Hanks’ balanced salt solution in the receiver on the BL side showed that phloroglucinol was detected in all experimental sections using eckols, and it's concentration increased with time over the course of the incubation. The other molecules corresponding to the unconjugated and conjugated metabolites of eckols were not detected in the AP and BL sides through the assays. These results suggest that eckols, including monomeric eckol, may be decomposed into phloroglucinol in the intestinal epithelium and the resulting phloroglucinol permeates to the BL side.

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