Prevalence and antimicrobial susceptibility patterns of Staphylococcus aureus/methicillin-resistant Staphylococcus aureus nasal carriage among Kabul University students

Nasal carriage of Staphylococcus aureus/Methicillin-resistant Staphylococcus aureus (S. aureus/MRSA) poses a major risk for the transmission and infection of this pathogen especially in hospital setting. The main objective of this study was to investigate the colonization of S. aureus/MRSA among healthy individuals and determine their susceptibility patterns to common antibiotics in Kabul. A total of 150 healthy participants have been included and nasal swabs were collected from all. Samples were cultured on appropriate and selective media for proper identification of S. aureus. Antibiotic susceptibility profiles were determined by the disc diffusion method and interpreted according to the Clinical & Laboratory Standards Institute (CLSI) protocols. The prevalence of S. aureus nasal colonization was 33.3% with 12.7% MRSA nasal carriage. The prevalence of S. aureus/MRSA colonization was not statistically significant according to gender (p=0.84) and age (0.18). All MRSA isolates were sensitive to rifampicin, linezolid and acid fusidic. Thirty-two percent of the S. aureus isolates were multi-drug resistant. Multi-drug resistance varied from resistance to at least three classes of antibiotics (14%) to maximum six classes of antibiotics (4%). This study revealed a high prevalence of S. aureus/MRSA nasal carriage among healthy individuals when compared to similar studies conducted elsewhere and is a reason of concern. Identifying and treating MRSA carriers, as well as responsible use of antibiotics is recommended.

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A cross sectional study to assess nasal carriage of methicillin resistant Staphylococcus aureus in healthcare professionals in a tertiary care hospital

Journal of the Pakistan Medical Association ◽

10.47391/jpma.062 ◽

2020 ◽

pp. 1-12

Author(s):

Fibhaa Syed ◽

Nasim Akhtar ◽

Mohammad Ali Arif ◽

Adil Ramzan ◽

Rauf Niazi ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Healthcare Workers ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care ◽

Nasal Carriage ◽

Cross Sectional Study ◽

Diffusion Method ◽

Cross Sectional ◽

Methicillin Resistant ◽

Nasal Swabs

Abstract Objective: To determine the nasal carriage of staphylococcus aureus and methicillin-resistant staphylococcus aureus among healthcare workers in a tertiary care setting. Methods: The cross-sectional study was conducted at the Pakistan Institute of Medical Sciences, Islamabad, Pakistan, from April to July 2018, and comprised healthcare workers at the institution. Nasal swabs were collected and cultured on Mannitol salt agar. Mannitol fermenting colonies which were gram-positive cocci, catalase-positive and coagulase-positive were identified as staphylococcus aureus. Antibiotic susceptibility test was performed by modified Kirby-Bauer disc diffusion method. Methicillin resistance was detected using cefoxitin disc diffusion method. Data was analysed using SPSS 23. Results: Of the 210 nasal swabs, 52(24.76%) had a staphylococcus aureus growth, and, of them, 15(7.1%) were methicillin-resistant. No association could be established with either any single category of healthcare worker or an inter-department variation (p>0.05). Likewise, there was no association with age, gender, duration of service, smoking, co-morbidities, use of antibiotics in the preceding six months, treating a patient with methicillin-resistant staphylococcus aureus in the preceding six months and hospitalisation in the preceding year (p>0.05). Conclusion: The frequency of nasal carriage of methicillin resistant staphylococcus aureus amongst healthcare workers was regardless of the nature of their professional engagement. Key Words: Methicillin resistant staphylococcus aureus, Nasal carriage, Continuous...

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PREVALENCE OF NASAL CARRIAGE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AMONG HEALTHCARE WORKERS IN THE DEPARTMENTS OF OTORINOLARYNGOLOGY AND DENTISTRY IN KYIV, UKRAINE

Wiadomości Lekarskie ◽

10.36740/wlek202012101 ◽

2020 ◽

Vol 73 (12) ◽

pp. 2563-2567

Author(s):

Aidyn G. Salmanov ◽

Taras P. Bondar ◽

Yaroslav V. Shkorbotun ◽

Evelina A. Chumak ◽

Volodymyr O. Shkorbotun ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Virulence Factor ◽

Healthcare Workers ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nasal Carriage ◽

Diffusion Method ◽

Methicillin Resistant ◽

Significant Difference ◽

Mrsa Strains ◽

Encoding Genes

The aim: To obtain the first estimates of the current prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) in the departments of Otorinolaryngology and Dentistry and to determine of genes virulence factors (Panton Valentine Leukocidine (PVL) genes). Materials and methods: We performed a multicenter cross-sectional study. The susceptibility to antibiotics was determined by disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing. The virulence factor encoding genes, mecA, lukS-lukF, were detected by Polymerase Chain Reaction (PCR). Results: Incidence rate of S. aureus nasal carriage among HCWs was 36.2%, whereas MRSA carriage was 17%. Prevalence of MRSA carriage rate was 34.9% in Otorhinolaryngology departments and 9.7% in Dentistry. PCR testing confirmed that all MRSA strains were mecA gene-positive. The virulence factor encoding genes were detected in 82.3% of the S. aureus isolates from HCWs. Among S.aureus, the lukS-lukF genes were detected in over 59% of the strains. The lukS-lukF genes were detected in 55.5% of MRSA and in 58.9% of MSSA strains. LukS-lukF genes were most commonly co-present in MRSA strains. No significant difference was detected between the occurrences of lukS-lukF genes (P > 0.05). Conclusions: Personnell in otorhinolaryngology and dentistry departments have a high rate of nasal colonization of MRSA. This carrier state may be an important risk factor for transmission MRSA from physicians and nurses to patients and vice-versa. Screening for MRSA nasal carriage of HCWs is a key element in enabling infection control measures and early therapeutic decisions.

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Prevalence of nasal carriage rate for methicillin-resistant Staphylococcus aureus and its antibiotic susceptibility profiles in health care workers at Nanakaly Hospital, Erbil, Iraq

Zanco Journal of Medical Sciences ◽

10.15218/zjms.2018.053 ◽

2018 ◽

Vol 22 (3) ◽

pp. 411-419 ◽

Cited By ~ 1

Author(s):

Nasik Dogramachy

Keyword(s):

Health Care ◽

Staphylococcus Aureus ◽

Health Care Workers ◽

Antibiotic Susceptibility ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nasal Carriage ◽

Carriage Rate ◽

Methicillin Resistant ◽

Care Workers ◽

Susceptibility Profiles

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The Relationship between Nasal Carriage of Staphylococcus aureus and Surgical Site Infections in a Hospital Center in Morocco

International Journal of Microbiology ◽

10.1155/2021/5585588 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Rachid Flouchi ◽

Abderrahim Elmniai ◽

Abdelaziz Hibatallah ◽

Karim Fahsi ◽

Ibrahim Touzani ◽

...

Keyword(s):

Public Health ◽

Risk Factors ◽

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Surgical Site Infections ◽

Nasal Carriage ◽

Diffusion Method ◽

Postoperative Phase ◽

Methicillin Resistant ◽

Hospital Center

Background. Surgical site infection is a major public health problem in the world. Nasal carriage is a major risk factor for the development of nosocomial Staphylococcus aureus infection, especially methicillin-resistant Staphylococcus aureus (MRSA). Our work aims to determine the prevalence of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and the associated risk factors and to evaluate their sensitivity to 27 antibiotics. Methods. A cross-sectional study was carried out on 100 patients, hospitalized in preoperative care of surgery units at the Taza Provincial Hospital Center in the Fez-Meknes region, from January to June 2019. Samples were taken from the patient’s anterior nostril using single-use sterile dry or wet cotton swabs and then analyzed in the Provincial Public Health Laboratory in Taza. The carriage of Staphylococcus aureus was studied by conventional bacteriological methods by spreading nasal swabs on Chapman culture medium, while antibiotic resistance was determined by the Mueller–Hinton agar disc diffusion method according to the recommendations described by the Antibiogram Committee of the French Society of Microbiology 2019 (CA SFM 2019). Results. Of the 84 patients found to be positive, 45.24% had coagulase-positive Staphylococcus aureus and 54.76% had coagulase-negative Staphylococcus. After surgery in the postoperative phase, 16 patients developed surgical site infections, of which two had a negative nasal culture and 14 had positive nasal culture. Among the Staphylococcus aureus-positive patients, 36.84% were colonized by a methicillin-resistant Staphylococcus aureus (MRSA) and 63.16% by a methicillin-sensitive Staphylococcus aureus (MSSA). Of these, 57.14% of MRSA colonized patients developed an infection of the surgical site and 42.85% showed no sign of SSI, while for patients colonized by MSSA, 16.67% developed SSI and 83.33% showed no sign of SSI. Moreover, children were the most affected by MRSA. Concerning antibiotic sensitivity, multiresistance of MRSA to more than 3 antibiotics has been found. Conclusion. To the best of our knowledge, this is the first study carried out in this hospital center with the aim of knowing the prevalence of nasal carriage of Staphylococcus aureus and MRSA and to identify the risk factors in order to prevent infections related to nasal carriage of Staphylococcus aureus and MRSA.

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Staphylococcus aureus nasal carriers and the prevalence of methicillin resistant Staphylococcus aureus among medical students

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20173004 ◽

2017 ◽

Vol 5 (7) ◽

pp. 3149 ◽

Cited By ~ 1

Author(s):

Baag Shaza R. ◽

Vishvesh P. Bansal ◽

Manjushree Bhalchandra ◽

Jyotsna Mishra

Keyword(s):

Staphylococcus Aureus ◽

Medical Students ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nasal Carriage ◽

Staphylococcal Infection ◽

Hospital Environment ◽

Diffusion Method ◽

Methicillin Resistant ◽

Nasal Swabs ◽

Hospital Acquired

Background: Staphylococcus aureus is a dynamic and adaptable bacterium that has an incredible talent to attain antibiotic resistance. Nasal colonisation of S.aureus increases with greater exposure of students to the hospital environment. The prevention of staphylococcal infection and reduction of spread and emergence of MRSA are essential. Medical students would be a key target group to introduce awareness of hospital-acquired infections. Therefore, the present study aims at understanding the prevalence of carrier rate of S.aureus and MRSA among Medical students during their clinical postings.Methods: Nasal swabs were taken from 150 medical students. Their Staphylococcus aureus nasal carriage was determined by plating on sheep blood agar and MRSA carriage by to Kirby Bauer Disc Diffusion method using Cefoxitin disc (30 µg).Results: The present study showed a 40% nasal carriage of S.aureus amongst medical students. Of the 60 S. aureus isolates 28%, 36% and 56% isolates were from 1st, 2nd and 3rd year respectively. The colonisation rate of MRSA in the present study was found to be 12.66% amongst the medical students of 6%, 10%, 22% belonged to 1st, 2nd, and 3rd respectivelyConclusions: The nasal carriage of Staphylococcus aureus and methicillin resistant Staphylococcus aureus (MRSA) among medical students increases as their exposure to patient care increases. Medical students should be made aware of the risks of carrying S.aureus and MRSA and educated about the hand washing protocol and safety precautions to be followed while handling patients.

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A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Applied Sciences ◽

10.3390/app11073206 ◽

2021 ◽

Vol 11 (7) ◽

pp. 3206

Author(s):

Lorina I. Badger-Emeka ◽

Promise Madu Emeka ◽

Hairul Islam M. Ibrahim

Keyword(s):

Staphylococcus Aureus ◽

Mechanism Of Action ◽

Methicillin Resistant Staphylococcus Aureus ◽

Nigella Sativa ◽

Synergistic Effects ◽

Diffusion Method ◽

Methicillin Resistant ◽

Cell Wall Disruption ◽

Time Kill ◽

Insight Into

Methicillin-resistant Staphylococcus aureus (MRSA) infection is detrimental to hospitalized patients. With diminishing choices of antibiotics and the worry about resistance to colistin in synergistic combined therapy, there are suggestions for the use of herbal derivatives. This investigation evaluated the synergistic effects of Nigella sativa (NS) in combination with beta-lactam (β-lactam) antibiotics on extreme drug-resistant (XDR) MRSA isolates. NS concentrations of 10, 7.5, 5.0, 2.5, 1.0, and 0.1 µg/mL, alone and in combination with β-lactam antibiotics, were used to determine the antimicrobial susceptibility of MRSA isolates by the well diffusion method. Time–kill assays were performed using a spectrophotometer, with time–kill curves plotted and synergism ascertained by the fractional inhibitory concentration (FIC). Scanning and transmission electron microscopy were used to gain insight into the mechanism of action of treated groups. Isolates were inhibited by the NS concentrations, with differences in the zones of inhibition being statistically insignificant at p < 0.05. There were statistically significant differences in the time–kill assay for the MRSA isolates. In addition, NS combined with augmentin showed better killing than oxacillin and cefuroxime. The mechanism of action shown by the SEM and TEM results revealed cell wall disruption, which probably created interference that led to bacterial lysis.

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Detection of Methicillin Resistant Staphylococcus aureus in Dairy Products and Anterior Nares of Dairy Workers

Tribhuvan University Journal of Microbiology ◽

10.3126/tujm.v6i0.26585 ◽

2019 ◽

Vol 6 ◽

pp. 59-62

Author(s):

Ranjana K.C. ◽

Ganga Timilsina ◽

Anjana Singh ◽

Supriya Sharma

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Susceptibility ◽

Dairy Products ◽

Methicillin Resistant Staphylococcus Aureus ◽

Dairy Product ◽

Control Measures ◽

Diffusion Method ◽

Mannitol Salt Agar ◽

Methicillin Resistant ◽

Dairy Workers

Objectives: To isolate methicillin resistant Staphylococcus aureus (MRSA) from anterior nares of dairy workers and dairy products and assess the antibiotic susceptibility pattern of the isolates. Methods: Swab samples collected from anterior nares of dairy workers and dairy product (butter) were inoculated into mannitol salt agar and incubated at 37ºC for 24 hours. Identification was done based on colony characteristics, Gram's staining, catalase, oxidase and coagulase test. Antibiotic susceptibility testing was done by modified Kirby Bauer disc diffusion method. MRSA was confirmed by using cefoxitin disc. Results: A total of 109 S. aureus (98 from dairy workers and 11 from butter samples) were isolated. Out of them 32 MRSA were isolated from dairy workers and 4 from butter samples. The association between age group and MRSA was found insignificant (p = 0.115). The association of MRSA between male and female workers was found significant (>0.05). About 86% of the MRSA isolates were susceptible to Gentamicin (86.11%) followed by Ciprofloxacin (77.78%). Conclusion: Detection of MRSA among dairy workers and dairy products warrants proper handling and adequate control measures to prevent transmission of MRSA from dairy industry.

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