scholarly journals Validation of two human point-of-care glucometers for glucose concentration determination in rats

2021 ◽  
pp. 52-55
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Point Of Care ◽  
Test Tube ◽  
Small Sample ◽  
Diagnostic Process ◽  
Albino Rats ◽  
Sprague Dawley ◽  
Glucose Determination ◽  
Test Kit

Glucose disorders associated with critical illness are common in veterinary medicine and availability of efficient, easy and affordable diagnostic process is vital in combating the menace. Point-of-care glucometers readily come to mind in achieving this goal considering their importance in research and critical case management in veterinary clinics as they are cheap, user friendly, can use small sample quantity and quick generation of results. However, there have been concerns about generation of erroneous results in certain species. This study compared the accuracy and agreement of two point-of-care glucometers for blood glucose determination in rats. Blood samples were obtained from 20 healthy Sprague-Dawley albino rats. Blood (2 ml) was collected through the retro-bulbar plexus and two glucometers were used to determine the blood glucose concentrations immediately. Thereafter, 1 ml was put into a clean test tube treated with ethylene diamine tetracetic acid and the remaining 1 ml was also put into another test tube without anticoagulant. The samples were processed appropriately to harvest the plasma and serum. Blood glucose test kit was used to measure glucose concentrations in plasma and serum by the glucose oxidase method. Data generated were analysed using one way analysis of variance. Results showed that the mean value generated by one glucometer was relatively comparable with the values generated by the laboratory methods while the other significantly overestimated the glucose concentration. This underscores the importance of validation of glucometers before use in any species as unvalidated glucometers can lead to erroneous research conclusions and clinical decisions with dire consequences

scholarly journals Blood Glucose Concentration Does Not Affect Outcome in Brain Trauma: A 31P MRS Study

1997 ◽  
Vol 17 (1) ◽  
pp. 50-53 ◽  
Author(s):  
Robert Vink ◽  
Elke M. Golding ◽  
John P. Williams ◽  
Tracy K. McIntosh
Keyword(s):  
Blood Glucose ◽  
Brain Injury ◽  
Motor Function ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Traumatic Injury ◽  
Neurologic Outcome ◽  
Resonance Spectroscopy ◽  
Sprague Dawley ◽  
Radioactive Phosphorus

Effects of blood glucose concentration on biochemical and neurologic outcome following lateral fluid percussion-induced traumatic injury of moderate severity (2.8 atm) in rats were studied using radioactive phosphorus (31P) magnetic resonance spectroscopy (MRS) and a battery of tests designed to evaluate posttraumatic neurologic motor function. Prior to injury, male Sprague-Dawley rats (n = 18) were randomly assigned to receive either dextrose, 2 ml 50% (wt/vol), zinc insulin (10 IU/kg) or no treatment, thus dividing the animals into hyperglycemic, hypoglycemic, and normoglycemic groups, respectively. Animals were then injured, monitored for 4 h by 31P MRS before being allowed to recover, and assessed for posttraumatic motor function. Following brain injury, there was no difference in brain intracellular pH between groups over the 4-h posttraumatic MRS monitoring period. Similarly, intracellular free magnesium, cytosolic phosphorylation potential, and neurologic outcome posttrauma were not significantly different between groups. We conclude that, unlike models of ischemia, blood glucose concentration may not be a significant factor affecting outcome in traumatic brain injury.

scholarly journals Knowledge of Validation Status of Point-of-care Glucometers among Veterinarians and Veterinary Technologists in Nigeria

2021 ◽  
Vol 41 (3) ◽  
pp. 214-222
Author(s):  
C.O Okorie-Kanu ◽  
O.J Okorie-Kanu ◽  
C.A Akwuobu ◽  
E.V Tizhe ◽  
R.E Antia
Keyword(s):  
Blood Glucose ◽  
Point Of Care ◽  
Academic Staff ◽  
Laboratory Methods ◽  
Public And Private ◽  
Glucose Determination ◽  
Blood Glucose Determination ◽  
Structured Questionnaire

Point-of-care glucometers (PCGs) have of recent almost replaced the conventional laboratory methods of blood glucose determination in animals. This study evaluated the level of awareness and knowledge about the use of handheld PCGs among veterinarians and veterinary technologists. Respondents to a structured questionnaire included academic staff and laboratory technologists from veterinary schools and public and private veterinarians across Nigeria. Design of the questions progressed from whether one had ever used a PCG before or not, how they knew about the PCG, the brands used, for what purposes and on which animals. Results showed that out of 209 respondents, 75 (36%) had used PCGs. Of this number, 37 (49.33%) used PCGs for research purposes, while 36 and 6.67% had used the PCGs for diagnosis of glucose disorders in animals and for both research and diagnostic purposes, respectively. The distribution of respondents that knew about the validation status of the PCGs used was 2.67%. As values generated by each PCG vary significantly in different species, there may be chances of reporting erroneous research conclusions as well as misdiagnosis of glucose disorders with consequent erroneous therapies in such species. Keywords: Point-of-care Glucometers, Validation status, Veterinarians.

scholarly journals ANALYTICAL COMPARISON BETWEEN SPECTROPHOTOMETER AND PORTABLE GLUCOMETER FOR MEASUREMENT OF BLOOD GLUCOSE IN HORSE

2019 ◽  
Author(s):  
Nanoshe Taye Jima ◽  
Yoseph Cherinet Megerssa
Keyword(s):  
Blood Glucose ◽  
Clinical Decision Making ◽  
Clinical Laboratory ◽  
Addis Ababa ◽  
Point Of Care ◽  
Clinical Decision ◽  
Mean Value ◽  
P Value ◽  
Glucose Determination ◽  
Spectrophotometric Methods

Abstract Objective: Analytical methods comparison for the determination of blood glucose are essential in clinical laboratory practice as it improves the quality of health care through accurate and reliable clinical decision making. Therefore The study was done to assess the analytical performance between point-of-care glucometer and spectrophotometric methods for blood glucose determination. Results: Twenty paired samples from horses visiting SPANA clinic of college of veterinary medicine and agriculture Addis Ababa University was used. Data obtained from both instruments was compared. The results shows that the mean value of blood glucose concentrations were higher in by the glucometric method (106mg/dl) than by the spectrophotometric (73mg/dl) with the calculated t-statistic significantly different p-value of 0.000. This study showed the clinical inaccuracy of the glucometer over the spectrophotometer.

Performance and Limitations of Point-of-Care Glucose Meters for Measuring Blood Glucose Concentration in Patients with Critical Medical Conditions

2015 ◽  
Vol 144 (suppl 2) ◽  
pp. A048-A048
Author(s):  
Lei Zhang ◽  
Carol Schwartz ◽  
Diping Wang ◽  
Dima El Zein ◽  
Keith Krabill ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Point Of Care ◽  
Medical Conditions

Laboratory Evaluation of the GlucocardTM Blood Glucose Test Meter

1992 ◽  
Vol 38 (10) ◽  
pp. 2093-2095 ◽  
Author(s):  
B D Lewis
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Test Strip ◽  
Sample Volume ◽  
Small Sample ◽  
Laboratory Evaluation ◽  
Self Monitoring ◽  
Blood Glucose Test ◽  
Blood Glucose Concentrations

Abstract The Glucocard (Kyoto Daiichi Kagaku) blood glucose meter is designed for self-monitoring of blood glucose concentrations in capillary blood through use of an electrochemical test strip. Evaluated in this laboratory, the Glucocard had CVs of 4.6%, 6.6%, and 3.5% at blood glucose concentrations of 2.4, 4.1, and 18.9 mmol/L, respectively. The meter's response varied linearly with blood glucose concentration between 2.2 and 27.8 mmol/L. Hemolysis, urate, ascorbate, and acetaminophen interfered by > 5%. Different hematocrits, in the range 0.20-0.70, did not affect the measured glucose concentration. Comparison with glucose results measured in whole blood with a NOVA Stat Profile 5 instrument yielded the following: Glucocard = 0.898 NOVA--0.184 (r = 0.995). The main advantages of the Glucocard are its small sample volume (5 microL), wide linear range, and fully automated sample-handling steps, which reduce user-related variability.

scholarly journals Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

2021 ◽  
Vol 14 (1) ◽  
pp. 065-070
Author(s):  
Ekpe IP ◽  
Eze KA ◽  
Amaechi Dennis
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Solanum Lycopersicum ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Solanum Melongena ◽  
Negative Control ◽  
Albino Rats ◽  
Solanum Lycopersicon ◽  
Treatment Groups

Lead is one of the heavy metal humans are often expose to either through food, cosmetics or environment. This study is aimed at investigating the positive effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot) on blood glucose concentration, body weight, and feeding habits of albino rats on lead toxicity. In this study 35 albino rats weighing 80-120g were grouped into five. Group 1 served as normal control, group 2 negative control. The remaining three groups served as treatment groups 1, 2 and 3. All groups except group 1 (normal control) were administered 50mg/kg of lead acetate. Treatment groups were administered 200mg/kg of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot). Group 3 was administered Solanum malongen and Daucus carrots subsp.sativus while group 4 was given Daucus carrots subsp.sativus and solanum lycopersicon. Group 5 was administered Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon. Changes in blood glucose concentration, body weight, feed and water consumption recorded at intervals. The result showed a significant decrease (P<0.05 and P<0.01) in blood glucose concentration in treatment groups compared to negative control. There was also significant increase (P>0.05, P>0.01 and P>0.001) in body weight and feed consumed in treatment groups compared to negative control. Combined therapy of any two of Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon juice can help in the management of hyperglycemia and reverse internal abnormality or injury resulting in weight loss in lead induced toxicity.

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