Sperm motility and velocities of Characiformes fishes in different times post-activation

The objective of the current study was to observe the performance kinetics (motilities and velocities) of the spermatozoa from Prochilodus brevis (curimatã), Colossoma macropomum (tambaqui) and Piaractus brachypomus (pirapitinga) species in different times post-activation. The sperm of P. brevis, C. macropomum and P. brachypomus species were collected after hormonal induction with carp pituitary extract. The samples with not contamination with water, urine or feces had motility subjective, morphology, osmolality and concentration analyzed. The samples selected were analyzed with Sperm Class Analyzer. Spermatozoa motility and velocities were captured at 10, 30, 60 and 120 s post-activation. No significant differences in total motility of P. brevis spermatozoa were observed between 10 s and 30 s post-activation. However, significant reduction was observed in 60 s. This reduction was more accentuated after 120 s. The same pattern of spermatozoa motility decline happened for C. macropomum and P. brachypomus. Velocities also followed the same pattern for the three species. There was significant reduction in velocities after 30 s; this reduction was more significant after 60 s. There was no significance difference between 60 s and 120 s post-activation. Sperm of C. macropomum and P. brachypomus show satisfactory sperm quality up to 60 s after activation. On the other hand, sperm of P. brevis up to 120 s after activation. These findings show that the rate of sperm motility in different times post activation is change for each species tested.

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294 EFFECT OF SPERMATOZOA MOTILITY ON TRANSFERABLE EMBRYO RATE OF SUPEROVULATED JAPANESE BLACK CATTLE

Reproduction Fertility and Development ◽

10.1071/rdv21n1ab294 ◽

2009 ◽

Vol 21 (1) ◽

pp. 244

Author(s):

K. Hayama ◽

M. Takeuchi ◽

A. Ideta ◽

M. Urakawa ◽

M. Sasatani ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Sperm Concentration ◽

Electronic Systems ◽

Embryo Production ◽

Spermatozoa Motility ◽

Multiple Comparison Test ◽

Medical Electronic ◽

D 20

Sperm motility is known to affect fertilization; however, little is known about the relationship between frozen–thawed sperm motility and in vivo fertilization following superovulatory treatment. The objective of this study was to evaluate a sperm function test as potential predictors of embryo production following superovulatory treatment in cattle. Two to five batches of semen (Japanese black bull, n = 4, A to D) were diluted with egg york-citrate-glycerol in 0.5 mL plastic straws, and they were stored in liquid nitrogen until analyzed. Frozen–thawed spermatozoa were evaluated for motility {motile sperm concentration (MSC, million mL–1), progressive MSC (PMSC, million mL–1) and velocity (μm s–1)} using a sperm quality analyzer for bulls (SQA-Vb, Medical Electronic Systems, Caesarea, Israel). Each sample of 20 μL aspirated into the disposable capillary, was inserted into SQA-Vb. Measurements were displayed within 75 s. Intra-assay CVs of MSC, PMSC, and velocity were 14.2, 7.3 and 7.5%, respectively. Inter-assey CVs of them were 13.5, 3.9 and 4.3% respectively. Superstimulated donors (Japanese black cows, n = 161) were artificially inseminated with one dose of frozen–thawed semen (bull A = 74, B = 46, C = 21 and D = 20). The proportion of transferable embryo (IETS grade 1 to 3) was examined on day 7 (day 0 = estrus). Data were analyzed using ANOVA followed by Scheffe multiple comparison test, and Fisher’s z-transformation. MSC, PMSC and velocity values differed significantly among each bull. The values of bull A were much lower than those of the other bulls. The proportion of transferable embryos produced by bull A was significantly lower than that of other bulls (P < 0.05, Table 1). Correlations showed significant association between MSC and proportion of transferable embryos (r = 0.99, P < 0.01). We conclude that bovine sperm motility using a SQA-Vb is a useful predictor of embryo production following superovulatory treatment. Table 1.Relationship between sperm motility and proportion of transferable embryo

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Mouse sperm delivery via cauda epididymis without transport medium

10.7287/peerj.preprints.2542v1 ◽

2016 ◽

Author(s):

Man-Xi Jiang ◽

Mei-Shan Wang ◽

Xiang-Hong Ou ◽

Xue-Jin Chen ◽

Yan Zhu

Keyword(s):

Embryo Development ◽

Sperm Motility ◽

Room Temperature ◽

Sperm Quality ◽

Mouse Sperm ◽

Spermatozoa Motility ◽

Transport Medium ◽

Fertilization Rates ◽

Progressive Motility

This study was aimed to investigate the effects of room temperature (RT, 20-25 oC) and absence of medium during cauda epididymis transport on spermatozoa quality, fertility and embryo development. In the first experiment, fresh sperm from one side of cauda epididymis was used for in vitro fertilization, and another side was delivered at RT or 4-8 oC either with or without M2. In the second experiment, each side of cauda epididymis obtained from the same mouse was individually delivered at RT or 4 oC with or without M2. Finally, sperm motility, progressive motility scores and fertility of fresh spermatozoa or those from transported cauda epididymis, and IVF embryo development were evaluated. Progressive motility scores and fertilization rates were higher in fresh spermatozoa than transported sperm; sperm motility of transported cauda epididymis at 4-8 oC was comparable to fresh spermatozoa, but spermatozoa motility of transported cauda epididymis at RT was inferior to fresh spermatozoa. Spermatozoa motillty of transported cauda epididymis at 4-8 oC with transport medium was much higher than that without transport medium; absence of transport medium did not affect sperm motility of transported cauda epididymis at 4-8 oC but affected sperm motility of transported cauda epididymis at RT. Sperm quality from transported cauda epididymis can be efficiently kept at 4-8 oC, and cauda epididymis transport at 4-8 oC without M2 is more beneficial on keeping their fertility. Moreover cauda epididymis transport at RT without medium could sufficiently produce embryos for obtainning live offsprings.

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Monitoring the time-grade required for extrusion of oocytes reproductive from colossoma macropomum

Scientific Electronic Archives ◽

10.36560/1362020946 ◽

2020 ◽

Vol 13 (6) ◽

pp. 57

Author(s):

D. Sevignani ◽

E. Buzzacaro ◽

N. B. Fortuna

Keyword(s):

Body Weight ◽

Water Temperature ◽

Strong Influence ◽

Pituitary Extract ◽

Artificial Reproduction ◽

Colossoma Macropomum ◽

Hormonal Induction ◽

Individual Features ◽

The Moment

The water temperature of the tanks of the breeding herds exerts a strong influence on the process of artificial reproduction. Its monitoring through the hour-degree, serves to assist in the moment to be made the extrusion of the oocytes. The objective was to monitor the time-degree that Colossoma macropomum females need after the second dose of the hormonal induction protocol, until the time of extrusion of their oocytes. The analysis was carried out at the Fish Fishery in the municipality of Sorriso-MT. Nine breeding women were submitted to the protocol of hormonal induction with pituitary extract of carp being administered in two doses. In the former, the dose of 0.5 mg / kg was used and the second dose was 5 mg / kg body weight, with an interval of 12 hours. From the second dose, the temperature in degrees centigrade of the water from the tanks was monitored hourly until the time of oocyte extrusion. Breeders had muscle tremors, swimming in circles, and were more agitated nine hours after the second dose. The oocytes had a yellowish-brown appearance, and some had individual features. The sum of the temperature of the water in hours-degree obtained was of 243. The temperature of the water did not present variation, maintaining itself at 27 ° C. It is concluded that an hour-degree is necessary for an extrusion of oocytes in some of the things you can find in Colossoma Macropomum of 243, agreeing with the limits proposed in the literature.

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Boar sperm quality in relation to presence of sp32-like protein in spermatozoa - preliminary studies

Bulletin of the Veterinary Institute in Pulawy ◽

10.1515/bvip-2015-0041 ◽

2015 ◽

Vol 59 (2) ◽

pp. 279-286 ◽

Cited By ~ 1

Author(s):

Aleksandra Orzołek ◽

Paweł Wysocki ◽

Jerzy Strzeżek ◽

Magdalena Koziorowska-Gilun ◽

Anna Dziekońska ◽

...

Keyword(s):

Sperm Motility ◽

Seminal Plasma ◽

Semen Quality ◽

Sperm Quality ◽

Total Antioxidant Status ◽

Atp Content ◽

Large White ◽

Sod Activity ◽

Spermatozoa Motility ◽

Total Antioxidant

Abstract The aim of the study was to analyse sperm proteomes of ejaculates from Polish Large White (PLW) and Polish Landrace (PL) boars and to identify differences which putatively influence semen quality. Spermatozoa protein profiles were analysed by electrophoretic methods followed by selected techniques to evaluate semen quality on the following factors: sperm motility, lipid peroxidation levels (MDA production), ATP content, activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS) of seminal plasma. A protein with an estimated molecular weight of 30 kDa was found in spermatozoa of selected ejaculates. Mass spectrometry demonstrated that this polypeptide is most similar to proacrosin binding protein (sp32). The presence of the protein was more frequently observed in sperm extracts obtained in spring-summer period. Ejaculates containing sp32-like protein demonstrated significantly higher spermatozoa motility, lower inhibition of MDA production by seminal plasma, and higher SOD activity in seminal plasma. Boar semen which included sp32-like protein also demonstrated lower ATP levels in spermatozoa as well as higher TAS and lower TOS of seminal plasma, though the differences were not statistically significant. Ejaculates from PLW boars, with sp32-like protein present in sperm, were characterised by significantly higher sperm motility, lower ATP content in spermatozoa, and higher TAS of seminal plasma. The diminished parameters of semen quality were observed in ejaculates from PL boars that also contained the discussed protein, but the differences were not statistically significant. These findings suggest that the presence of sp32-like protein in boar spermatozoa could influence semen quality

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Mouse sperm delivery via cauda epididymis without transport medium

10.7287/peerj.preprints.2542 ◽

2016 ◽

Author(s):

Man-Xi Jiang ◽

Mei-Shan Wang ◽

Xiang-Hong Ou ◽

Xue-Jin Chen ◽

Yan Zhu

Keyword(s):

Embryo Development ◽

Sperm Motility ◽

Room Temperature ◽

Sperm Quality ◽

Mouse Sperm ◽

Spermatozoa Motility ◽

Transport Medium ◽

Fertilization Rates ◽

Progressive Motility

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A study of cometary eruptions through OH radio-lines

International Astronomical Union Colloquium ◽

10.1017/s025292110003150x ◽

1999 ◽

Vol 173 ◽

pp. 249-254

Author(s):

A.M. Silva ◽

R.D. Miró

Keyword(s):

Short Duration ◽

Growth Curves ◽

The Other ◽

Initial Mass ◽

Radio Lines ◽

Other Hand ◽

Sudden Rise

AbstractWe have developed a model for theH2OandOHevolution in a comet outburst, assuming that together with the gas, a distribution of icy grains is ejected. With an initial mass of icy grains of 108kg released, theH2OandOHproductions are increased up to a factor two, and the growth curves change drastically in the first two days. The model is applied to eruptions detected in theOHradio monitorings and fits well with the slow variations in the flux. On the other hand, several events of short duration appear, consisting of a sudden rise ofOHflux, followed by a sudden decay on the second day. These apparent short bursts are frequently found as precursors of a more durable eruption. We suggest that both of them are part of a unique eruption, and that the sudden decay is due to collisions that de-excite theOHmaser, when it reaches the Cometopause region located at 1.35 × 105kmfrom the nucleus.

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Closing the GAP—A 5Å Scanning Microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100061938 ◽

1969 ◽

Vol 27 ◽

pp. 6-7

Author(s):

A. V. Crewe

Keyword(s):

Normal Form ◽

The Other ◽

Resolving Power ◽

Scanning Microscope ◽

Conventional Microscope ◽

Other Hand ◽

Closing The Gap ◽

Thermal Sources ◽

Better Than ◽

Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

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Life Beyond 1MeV

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600000118 ◽

1980 ◽

Vol 38 ◽

pp. 30-33

Author(s):

K.H. Westmacott

Keyword(s):

Electron Microscopy ◽

Past Experience ◽

The Other ◽

Good Case ◽

Other Hand ◽

The U.S

Life beyond 1MeV – like life after 40 – is not too different unless one takes advantage of past experience and is receptive to new opportunities. At first glance, the returns on performing electron microscopy at voltages greater than 1MeV diminish rather rapidly as the curves which describe the well-known advantages of HVEM often tend towards saturation. However, in a country with a significant HVEM capability, a good case can be made for investing in instruments with a range of maximum accelerating voltages. In this regard, the 1.5MeV KRATOS HVEM being installed in Berkeley will complement the other 650KeV, 1MeV, and 1.2MeV instruments currently operating in the U.S. One other consideration suggests that 1.5MeV is an optimum voltage machine – Its additional advantages may be purchased for not much more than a 1MeV instrument. On the other hand, the 3MeV HVEM's which seem to be operated at 2MeV maximum, are much more expensive.

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Can the Academic Self-Concept be Positive and Realistic?

Zeitschrift für Pädagogische Psychologie ◽

10.1024/1010-0652.19.3.132 ◽

2005 ◽

Vol 19 (3) ◽

pp. 129-132 ◽

Cited By ~ 6

Author(s):

Reimer Kornmann

Keyword(s):

Academic Performance ◽

Opposite Effect ◽

Self Esteem ◽

Point Of View ◽

The Other ◽

Self Concept ◽

High Ability ◽

Self Image ◽

Other Hand

Summary: My comment is basically restricted to the situation in which less-able students find themselves and refers only to literature in German. From this point of view I am basically able to confirm Marsh's results. It must, however, be said that with less-able pupils the opposite effect can be found: Levels of self-esteem in these pupils are raised, at least temporarily, by separate instruction, academic performance however drops; combined instruction, on the other hand, leads to improved academic performance, while levels of self-esteem drop. Apparently, the positive self-image of less-able pupils who receive separate instruction does not bring about the potential enhancement of academic performance one might expect from high-ability pupils receiving separate instruction. To resolve the dilemma, it is proposed that individual progress in learning be accentuated, and that comparisons with others be dispensed with. This fosters a self-image that can in equal measure be realistic and optimistic.

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Stories and the Self

Journal of Media Psychology Theories Methods and Applications ◽

10.1027/1864-1105/a000255 ◽

2020 ◽

Vol 32 (2) ◽

pp. 47-58 ◽

Cited By ~ 1

Author(s):

Stefan Krause ◽

Markus Appel

Keyword(s):

Meta Analysis ◽

The Self ◽

The Other ◽

Lower Degree ◽

Contrast Effects ◽

Self Perception ◽

Self Perceptions ◽

Other Hand ◽

The One ◽

Implicit And Explicit

Abstract. Two experiments examined the influence of stories on recipients’ self-perceptions. Extending prior theory and research, our focus was on assimilation effects (i.e., changes in self-perception in line with a protagonist’s traits) as well as on contrast effects (i.e., changes in self-perception in contrast to a protagonist’s traits). In Experiment 1 ( N = 113), implicit and explicit conscientiousness were assessed after participants read a story about either a diligent or a negligent student. Moderation analyses showed that highly transported participants and participants with lower counterarguing scores assimilate the depicted traits of a story protagonist, as indicated by explicit, self-reported conscientiousness ratings. Participants, who were more critical toward a story (i.e., higher counterarguing) and with a lower degree of transportation, showed contrast effects. In Experiment 2 ( N = 103), we manipulated transportation and counterarguing, but we could not identify an effect on participants’ self-ascribed level of conscientiousness. A mini meta-analysis across both experiments revealed significant positive overall associations between transportation and counterarguing on the one hand and story-consistent self-reported conscientiousness on the other hand.

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