Detection of anti-Leptospira antibodies in domestic captive primates from Acre, Brazil

Because the Wild Animals Screening Center (CETAS) focuses intensely on restoration and maintenance of primate health and diversity in the Amazon region, detection of potential threats to animal health is crucial. In this view, neglected infections such as leptospirosis are of particular interest. In this report, we have studied the seroreactivity of anti-Leptospira antibodies from primate blood samples collected at CETAS between September 2011 and September 2012. Of the 14 animals investigated by the microscopic agglutination test using live antigens, five (35.7%) were reactive against serovars Celledoni (1), Hebdomadis (1), Icterohaemorrhagiae (2), and Sejroe (1). The results indicate that it is important to identify the contact of these animals with possible pathogens in order to ensure reintroduction of healthy animals into the wild and further understand the disease epidemiology in animals under captivity, gathering, and voluntary surrender of domestic captivity in Acre, Brazil.

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Pigs leptospirosis at the territory of Belgrade

Veterinarski glasnik ◽

10.2298/vetgl1406331v ◽

2014 ◽

Vol 68 (5-6) ◽

pp. 331-338

Author(s):

Dragica Vojinovic ◽

Ana Vasic ◽

Dubravka Jovicic ◽

Bosiljka Djuricic ◽

Zivka Ilic

Keyword(s):

Agglutination Test ◽

Microscopic Agglutination Test ◽

Blood Samples ◽

Pig Farms ◽

Very High

Examinations of pig blood samples have been carried out at public pig farms in three Belgrade municipalities: Palilula, Surcin and Obrenovac. The number of tested blood serums of pigs at Palilula was 18990, in Surcin 7739 and in Obrenovac it was 8200. The number of positive blood serums on leptospirosis in pigs was 760, and out of that number the most positive samples (496) were in Surcin, followed by Palilula (217), and the least were found in Obrenovac (47). In the period from 2000 to 2009, there were tested 34929 blood serums of pigs from farms located at the territory of Belgrade. For the investigations there was used the microscopic agglutination test (MAT) on seven varieties of L. interrogans: L. pomona, L. icterohaemorrhagiae, L. grippotyphosa, L. sejroe, L. canicola, L. bataviae and L. australis. Seropositivity in pigs was determined in 760 blood serums, or in 2.17%. Leptospirosis prevalence was not very high, but it was constant, which indicates that leptospirosis infection maintained at the territory of Belgrade for ten years throughout our continual investigation.

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Prevalence and risk factors to Leptospira sp. infection in dogs attended at veterinary clinics in João Pessoa, Paraíba State, Northeastern Brazil

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/issn.1678-4456.bjvras.2018.144154 ◽

2018 ◽

Vol 55 (3) ◽

pp. e144154 ◽

Cited By ~ 1

Author(s):

Arthur Willian De Lima Brasil ◽

Diego Figueiredo Da Costa ◽

Carla Lauise Rodrigues Menezes Pimenta ◽

Roberta Nunes Parentoni ◽

Clebert José Alves ◽

...

Keyword(s):

Risk Factors ◽

Odds Ratio ◽

Agglutination Test ◽

Cutoff Point ◽

Northeastern Brazil ◽

Northeast Brazil ◽

Wild Animals ◽

Blood Samples ◽

Factors Associated ◽

The City

The aim of this study was to determine the prevalence and risk factors associated with Leptospira sp. in dogs attended at veterinary clinics in the city of João Pessoa, State of Paraíba, Northeast Brazil. A total of 384 blood samples from dogs from 34 veterinary clinics were used from April 2015 to May 2016. The diagnosis of Leptospira sp. was carried out through Microscopic Agglutination Test (MAT), using a collection of 20 pathogenic antigens and adopting a 1: 100 dilution as cutoff point. An epidemiological questionnaire was applied to the owners of the animals to obtain data to be used in the analysis of risk factors. The prevalence of seropositive animals was 11.7% (45/384), with reactions for serogroups Icterohaemorrhagiae (62.3%), Grippotyphosa (22.2%), Canicola (13.3%), Djasiman 2%) and Pomona (2.2%). The following risk factors were identified: age from 49 to 72 months (odds ratio = 2.74), Age > 72 months (odds ratio = 3.22), and monthly cleaning of the environment where the animals are kept (odds ratio = 10,70). It is concluded that dogs attended at veterinary clinics in João Pessoa are exposed to infection by Leptospira sp., with predominance of serogroups kept by wild animals, and it is suggested that the periodic cleaning of the environment where the animals are kept.

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Prevalence and risk factors for Leptospira exposure in New Zealand veterinarians

Epidemiology and Infection ◽

10.1017/s0950268815000515 ◽

2015 ◽

Vol 143 (10) ◽

pp. 2116-2125 ◽

Cited By ~ 13

Author(s):

J. M. SANHUEZA ◽

C. HEUER ◽

P. R. WILSON ◽

J. BENSCHOP ◽

J. M. COLLINS-EMERSON

Keyword(s):

Risk Factors ◽

New Zealand ◽

Confidence Interval ◽

Animal Species ◽

Significant Risk ◽

Positive Association ◽

Agglutination Test ◽

Microscopic Agglutination Test ◽

Blood Samples ◽

Influenza Like Illness

SUMMARYThis study assessed seroprevalence and risk factors for Leptospira (serovars Hardjo, Pomona, Ballum, Copenhageni, Tarassovi) exposure in New Zealand veterinarians. Veterinarians (n = 277) at one of two conferences were voluntarily enrolled and blood samples taken. Microscopic agglutination test (MAT) titres ⩾48 were considered seropositive. Fourteen veterinarians (5·1%, 95% confidence interval 2·8−8·3) were seropositive to Leptospira. Home slaughter of cattle or pigs were significant risk factors for Leptospira exposure. There were no clear relationships between the animal species handled at work and serostatus. However, veterinarians spending a ‘mid to high’ proportion of their time (>50% to ⩽75%) with pets had higher odds of being seropositive than those not working with pets. A borderline positive association (P = 0·09) was observed between seropositivity and clinical influenza-like illness (⩾3 days off work) in the 18 months before the study. Assuming causality, this suggests that 8·3% of these cases may be attributed to Leptospira exposure.

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Correlation Between Serology Test Result of Leptospira sp. With The Representation of Histopathological Lesions on The Cattle Kidneys

Medicra (Journal of Medical Laboratory Science/Technology) ◽

10.21070/medicra.v4i1.1405 ◽

2021 ◽

Vol 4 (1) ◽

pp. 30-34

Author(s):

Asih Rahayu ◽

Yos Adi Prakoso ◽

Kurnia Desiandura

Keyword(s):

Serological Test ◽

Animal Health ◽

Agglutination Test ◽

Microscopic Agglutination Test ◽

Coefficient Corrélation ◽

Cattle Serum ◽

Serology Test ◽

Spearman Test ◽

Test Result ◽

Histopathological Lesion

Leptospirosis is an eminent diseases among human and animal health. As a zoonosis disease, the occurrence of leptospirosis is not clearly understood in animal. Furthermore, the lesion caused by Leptospira sp. is not well demonstrated. This study aimed to analyze the correlation between the result of serological test using microscopic agglutination test (MAT) and the representation of histopathological lesion in kidney from the cattle. This study used 28 samples consist of cattle serum and kidney organs. The serum was tested using MAT and kidney was tested using histopathology. The data was reported semi quantitatively and tested using Spearman test. The result showed that there is no correlation between the result of serological test to the representation of histopathological lesion from the kidney of cattle. It is supported by the coefficient correlation (0,05) and probability value p=0,78 (p≥0,05). In conclusion, the result of Leptospira sp. serological test either seropositive or seronegative uncorrelated to the representation of histopathological lesion from the cattle kidney.

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Serological survey of anti-Leptospira spp. antibodies in Barbary sheep (Ammotragus lervia) at the Curitiba Zoo, southern Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-4327 ◽

2018 ◽

Vol 38 (1) ◽

pp. 143-146

Author(s):

Vivien M. Morikawa ◽

Cristina K. Zimpel ◽

Igor A.D. Paploski ◽

Mariana Kikuti ◽

Vanessa Castro ◽

...

Keyword(s):

Environmental Exposure ◽

Preventive Measures ◽

Agglutination Test ◽

Microscopic Agglutination Test ◽

Wild Animals ◽

Serological Survey ◽

Southern Brazil ◽

Ammotragus Lervia ◽

Barbary Sheep ◽

Leptospira Spp

ABSTRACT: Leptospirosis is a worldwide zoonosis, affecting humans, domestic and wild animals. The present study aimed to evaluate prevalence of anti-Leptospira spp. antibodies in Barbary sheep at the Curitiba zoo. Microscopic agglutination test (MAT) was performed using 17 serogroups. Antibodies against Leptospira spp. were observed in 23.5% samples and Icterohaemorrhagiae was the only prevalent serogroup. The presence of anti-Leptospira antibodies in Barbary sheep indicates exposure to leptospires; thus monitoring and preventive measures are necessary in zoo’s captive animals, since they can act as sentinels of environmental exposure in an area with high movement of people.

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Clinical and serological evaluation of Leptospirosis in Puducherry, India

The Journal of Infection in Developing Countries ◽

10.3855/jidc.384 ◽

2010 ◽

Vol 4 (03) ◽

pp. 139-143 ◽

Cited By ~ 4

Author(s):

Smita Benjamin Shekatkar ◽

Belgode Narasimha Harish ◽

Godfred Antony Menezes ◽

Subhash Chandra Parija

Keyword(s):

Zoonotic Disease ◽

Agglutination Test ◽

The Other ◽

Microscopic Agglutination Test ◽

Diagnostic Laboratory ◽

Seropositivity Rate ◽

Blood Samples ◽

Worldwide Distribution ◽

Igm Elisa ◽

Leptospira Serovars

Background: Leptospirosis is a zoonotic disease with a worldwide distribution. There is a paucity of available data about prevalence of this disease in Pondicherry. Our aim was to investigate the seropositivity rate of leptospirosis in suspected cases and also to identify the predominant serogroups present by performing Microscopic Agglutination Test (MAT). The other aim of this study was to compare the results of a commercially available IgM ELISA with that of MAT. Methodology: A total of 110 blood samples from patients suspected of leptospirosis were sent for diagnosis. These samples were subjected to IgM ELISA and the microscopic agglutination test (MAT). MAT was done using a panel of 12 Leptospira serovars. Results: MAT analysis of the 110 samples showed 40 (36%) to be positive. Antibodies were predominantly seen against serogroup Leptospira Icterohemorrhagiae (27%), followed by Pomona (17%), and Pyrogenes (12%). IgM ELISA done on these samples showed a positivity of 37% compared to MAT. Conclusion: This study reveals that the MAT test can be standardized in a diagnostic laboratory and used in conjunction with an IgM ELISA.

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Negative results for anti-Leptospira sp. agglutinins in free-living Iguanas (Iguana iguana) from the Amazon region of Brazil

Ciência Rural ◽

10.1590/0103-8478cr20170822 ◽

2018 ◽

Vol 48 (9) ◽

Author(s):

Thamillys Rayssa Marques Monteiro ◽

Katarine de Souza Rocha ◽

Gleiciane Schupp de Sena Mesquita ◽

Danniel Carvalho de Castro ◽

Stefânia Araújo Miranda ◽

...

Keyword(s):

Northern Region ◽

Amazon Region ◽

Agglutination Test ◽

Serological Diagnosis ◽

Metropolitan Region ◽

Free Living ◽

Iguana Iguana ◽

Blood Samples ◽

Negative Results ◽

Serological Studies

ABSTRACT: Although, serological studies for leptospirosis in iguanas have already been performed, there is no report in the Amazon region. Therefore, the present study aimed to investigate the presence of anti-Leptospira sp. in free living Iguanas-verdes (Iguana iguana) of Mangal das Garças Park, metropolitan region of Belém, Pará, Northern region of Brazil. Twenty-nine blood samples of Iguana-verde (I. iguana) were collected from Mangal das Garças Park. For the serological diagnosis of Leptospira sp. it was employed microscopic agglutination test (MAT) with 25 live antigens of Leptospira sp. as antigens. It was observed that all samples were non-reactive, suggesting no exposure to the agent.

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Presence of anti-Leptospira spp. antibodies in captive yellow-spotted river turtles (Podocnemis unifilis) in the Eastern Amazon

Ciência Rural ◽

10.1590/0103-8478cr20190088 ◽

2020 ◽

Vol 50 (1) ◽

Author(s):

Juliana Maria Santos Miranda ◽

Katarine de Souza Rocha ◽

Louysse Helene Monteiro ◽

Ianny Watuzy Monteiro Baia ◽

Thamillys Rayssa Marques Monteiro ◽

...

Keyword(s):

Amazon Region ◽

Agglutination Test ◽

Single Sample ◽

Microscopic Agglutination Test ◽

Serum Samples ◽

Podocnemis Unifilis ◽

Leptospira Spp ◽

River Turtles

ABSTRACT: We describe anti-Leptospira spp. agglutinin in yellow-spotted river turtles (Podocnemis unifilis)in the Amazon region. Ninety-eight serum samples from individuals housed at the Bosque Rodrigues Alves Zoobotanical Garden of Amazonia, Belém, PA, Brazil, were subject to the microscopic agglutination test (MAT) using 19 different Leptospira spp. antigen serogroups. Thirty-four of the 98 samples (35%) were reactive, with titers ranging from 100 to 3200, and eight 8 reactive samples (23.5%) co-agglutinated under two or more serovars.The most common serogroup was Hebdomadis (26.9%, 7/26), followed by Semaranga (23%, 6/26), Shermani (19.2%, 5/26), Djasiman (11.5%, 3/26), and Australis (7.7%, 2/26); Bataviae, Javanica, and Sejroewere represented by a single sample each (3.9%). The presence of turtles reactive to anti-Leptospira spp. antibodies implies exposure to the pathogen.

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Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

Clinical and Vaccine Immunology ◽

10.1128/cvi.00219-06 ◽

2006 ◽

Vol 13 (10) ◽

pp. 1166-1169 ◽

Cited By ~ 33

Author(s):

Stuart D. Blacksell ◽

Lee Smythe ◽

Rattanaphone Phetsouvanh ◽

Michael Dohnt ◽

Rudy Hartskeerl ◽

...

Keyword(s):

Diagnostic Accuracy ◽

Immunosorbent Assay ◽

Gold Standard ◽

Agglutination Test ◽

Immunoglobulin M ◽

Microscopic Agglutination Test ◽

Enzyme Linked Immunosorbent Assay ◽

Diagnostic Utility

ABSTRACT The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira “gold standard” microscopic agglutination test.

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