scholarly journals Correlation Between Serology Test Result of Leptospira sp. With The Representation of Histopathological Lesions on The Cattle Kidneys

2021 ◽  
Vol 4 (1) ◽  
pp. 30-34
Author(s):  
Asih Rahayu ◽  
Yos Adi Prakoso ◽  
Kurnia Desiandura
Keyword(s):  
Serological Test ◽  
Animal Health ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Coefficient Corrélation ◽  
Cattle Serum ◽  
Serology Test ◽  
Spearman Test ◽  
Test Result ◽  
Histopathological Lesion

Leptospirosis is an eminent diseases among human and animal health. As a zoonosis disease, the occurrence of leptospirosis is not clearly understood in animal. Furthermore, the lesion caused by Leptospira sp. is not well demonstrated. This study aimed to analyze the correlation between the result of serological test using microscopic agglutination test (MAT) and the representation of histopathological lesion in kidney from the cattle. This study used 28 samples consist of cattle serum and kidney organs. The serum was tested using MAT and kidney was tested using histopathology. The data was reported semi quantitatively and tested using Spearman test. The result showed that there is no correlation between the result of serological test to the representation of histopathological lesion from the kidney of cattle. It is supported by the coefficient correlation (0,05) and probability value p=0,78 (p≥0,05). In conclusion, the result of Leptospira sp. serological test either seropositive or seronegative uncorrelated to the representation of histopathological lesion from the cattle kidney.

scholarly journals Detection of anti-Leptospira antibodies in domestic captive primates from Acre, Brazil

2017 ◽  
Vol 38 (1) ◽  
pp. 301 ◽  
Author(s):  
Luciana Dos Santos Medeiros ◽  
Soraia Figueiredo de Souza ◽  
Yuri Karaccas de Carvalho ◽  
Maria Vânia França Ribeiro ◽  
Gabriel Martins ◽  
...  
Keyword(s):  
Animal Health ◽  
Amazon Region ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Wild Animals ◽  
Blood Samples ◽  
Disease Epidemiology ◽  
Region Detection

Because the Wild Animals Screening Center (CETAS) focuses intensely on restoration and maintenance of primate health and diversity in the Amazon region, detection of potential threats to animal health is crucial. In this view, neglected infections such as leptospirosis are of particular interest. In this report, we have studied the seroreactivity of anti-Leptospira antibodies from primate blood samples collected at CETAS between September 2011 and September 2012. Of the 14 animals investigated by the microscopic agglutination test using live antigens, five (35.7%) were reactive against serovars Celledoni (1), Hebdomadis (1), Icterohaemorrhagiae (2), and Sejroe (1). The results indicate that it is important to identify the contact of these animals with possible pathogens in order to ensure reintroduction of healthy animals into the wild and further understand the disease epidemiology in animals under captivity, gathering, and voluntary surrender of domestic captivity in Acre, Brazil.

scholarly journals Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

2006 ◽  
Vol 13 (10) ◽  
pp. 1166-1169 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Lee Smythe ◽  
Rattanaphone Phetsouvanh ◽  
Michael Dohnt ◽  
Rudy Hartskeerl ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Gold Standard ◽  
Agglutination Test ◽  
Immunoglobulin M ◽  
Microscopic Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Utility

ABSTRACT The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira “gold standard” microscopic agglutination test.

scholarly journals Leptospirosis serosurvey in bovines from Brazilian Pantanal using IGG ELISA with recombinant protein LipL32 and microscopic agglutination test

2007 ◽  
Vol 38 (4) ◽  
pp. 674-680 ◽  
Author(s):  
Renata Graça Pinto Tomich ◽  
Maria Rosa Quaresma Bomfim ◽  
Matilde Cota Koury ◽  
Aiesca Oliveira Pellegrin ◽  
Luiz Alberto Pellegrin ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Brazilian Pantanal

scholarly journals Kejadian Leptospirosis pada Anjing di Daerah Istimewa Yogyakarta (CASE OF CANINE LEPTOSPIROSIS IN THE CITY OF YOGYAKARTA)

2017 ◽  
Vol 18 (3) ◽  
pp. 403
Author(s):  
Guntari Titik Mulyani ◽  
Sri Hartati ◽  
Yuli Santoso ◽  
Kurnia Kurnia ◽  
Agung Budi Pramono ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Clinical Symptoms ◽  
Emerging Infectious Diseases ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Severe Illness ◽  
Leptospira Interrogans ◽  
Veterinary Research ◽  
History Of ◽  
The City

Leptospirosis is a zoonotic disease, which is caused by Leptospira interrogans. The incidence of leptospirosis in dogs varies according to region and season, and is considered as emerging infectious diseases in humans. Clinical symptoms of leptospirosis in dogs vary greatly, some dogs are asymptomatic, with mild symptoms, and others progress to severe illness until death. The study aims to determine cases of canine leptospirosis in Yogyakarta, and identify the serovar that infect them. A total of 20 dogs without symptoms, with mild symptoms, to severe symptoms of leptospirosis were collected their serum and further tested using Microscopic Agglutination Test (MAT) conducted at the Center for Veterinary Research (BBLitvet) Bogor. History of vaccination is recorded as a consideration in interpreting the MAT results. The results showed that three out of the 20 samples were positive leptospirosis. Of the three positive samples, one was identified as serovar bataviae, another one as serovar bataviae and tarrasovi, and the last as serovar bataviae, tarrasovi, ichterohaemorrhagiae, canicola, celledoni, pyrogenes, cynopteri, and rachmati, respectively. The three dogs with leptospirosis showed similar clinical symptoms i.e. Anorexia, lethargy, and fever. It can be concluded that there are cases of canine leptospirosis in Yogyakarta which is predominantly caused by Leptospira interrogans serovar bataviae. ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh Leptospira interrogans. Kejadian leptospirosis pada anjing bervariasi menurut wilayah dan musim, dan dianggap sebagai penyakit menular yang muncul pada manusia. Gejala klinis leptospirosis pada anjing sangat bervariasi, beberapa anjing tanpa menunjukkan gejala, dengan gejala ringan, dan yang lain melanjut menjadi penyakit yang parah sampai kematian. Penelitian ini bertujuan untuk mengetahui kasus leptospirosis pada anjing di Propinsi Daerah Istimewa Yogyakarta, dan mengidentifikasi serovar yang menginfeksinya. Sebanyak 20 ekor anjing tanpa gejala, dengan gejala ringan, sampai gejala parah leptospirosis diambil serumnya guna pemeriksaan Microscopic Agglutination Test (MAT) yang dilaksanakan di Balai Besar Penelitian Veteriner (BBLitvet), Bogor. Sejarah vaksinasi dicatat sebagai bahan pertimbangan dalam menginterpretasi hasil pemeriksaan MAT. Hasil penelitian menunjukkan bahwa tiga dari 20 sampel positif leptospirosis. Satu sampel positif terhadap serovar Bataviae, satu sampel positif terhadap serovar Bataviae dan Tarrasovi, dan satu sampel lagi positif terhadap serovar Bataviae, Tarrasovi, Ichterohaemorrhagiae, Canicola, Celledoni, Pyrogenes, Cynopteri, dan Rachmati. Anjing yang positif leptospirosis menunjukkan gejala klinis yang sama berupa anoreksia, kelemahan, dan demam. Dari hasil penelitian ini dapat disimpulkan bahwa terdapat kasus leptospirosis pada anjing di Yogyakarta yang penyebabnya dominan oleh Leptospira interrogans serovar Bataviae.

scholarly journals Use of saprophytic leptospira strains in the serodiagnosis of experimental leptospirosis in guinea-pigs (Cavia sp)

1988 ◽  
Vol 30 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Raul J. S. Girio ◽  
Luis A. Mathias
Keyword(s):  
Guinea Pigs ◽  
Buenos Aires ◽  
Sao Paulo ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Leptospira Interrogans ◽  
São Paulo ◽  
Cross Reactions ◽  
Single Antigen ◽  
Antibody Titres

The efficiency of four Leptospira biflexa strains (Buenos Aires, Patoc 1, Rufino and São Paulo) as single antigen in the serodiagnosis in guinea-pigs experimentally infected with seven Leptospira interrogans serovars (canicola, grippotyphosa, hardjo, icterohaemorrhagiae, pomona, tarassovi and wolffi) was evaluated by the microscopic agglutination test. The four saprophytic strains were not able to reveal antibody titres in sera of guinea-pigs experimentally infected with Leptospira interrogans. Serological cross-reactions were observed between strains Patoc 1 and São Paulo and between serovars wolffi and hardjo.

scholarly journals EVALUASI PERFORMA BOBOT BADAN DAN STATISTIK VITAL SAPI MADURA BERDASARKAN TAHUN KELAHIRAN

2017 ◽  
Vol 5 (2) ◽  
pp. 40
Author(s):  
Sulistyoningtyas I. ◽  
V.M. Ani Nurgiartiningsih ◽  
Gatot Ciptadi
Keyword(s):  
Body Weight ◽  
Body Length ◽  
Animal Health ◽  
Body Height ◽  
Animal Breeding ◽  
T Test ◽  
Female Cattle ◽  
Test Result

This research was done to compare performance of Madura cattle born in 2015 and 2016 at Unit of Technical for Animal Breeding and Animal Health Madura. Two hundred tails of Madura cattle that were one day old, 205 days old, and 365 days old were used in this research. They were offsprings of 12 tails of male cattle and 200 tails of female cattle. Variables observed were body weight, body height, body length, and heart girth. Data collected were analyzed by t test. Result of this research indicated that performance of Madura cattle that was one day old and 205 days old born in 2015 and in 2016 were not different (P>0.05). Performance of Madura cattle that was 365 days old born in 2015 and in 2016 were different (P<0.05). The performance of cattle born in 2015 versus in 2016 were 113.00±12.19 kg vs 120.00±10.86 kg for body weight (yearling weight),  110.00±6.81 cm vs 114.00±2.46 cm for body height, 103.00±6.70 cm vs 106.00±4.71 cm for body length, and 118.00±8.05 cm vs 120.00±5.91 cm for heart girth. It could be concluded that performance of Madura cattle that was 365 days old born in 2016 were higher than that was in 2015. Keywords: Madura cattle, Body weight, Bidy height, Body length, Heart girth

scholarly journals Leptospirosis

2016 ◽  
Vol 8 (3) ◽  
Author(s):  
Novie H. Rampengan
Keyword(s):  
Gold Standard ◽  
Case Fatality Rate ◽  
Severe Disease ◽  
Case Fatality ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Moderate Risk ◽  
Rat Urine ◽  
The World ◽  
Different Parts

Abstract: Leptospirosis is a zoonotic disease that usually occurs during the flood and is generally transmitted through rat urine. Indonesia is a country with a moderate risk of transmission of leptospirosis. Leptospirosis has a broad manifestation varying from self-limited to severe disease. The gold standard examination of leptospirosis is microscopic agglutination test. Diagnosis is divided into suspected, probable, and confirmed. Treatment consists of antibiotics and supportive agents. Generally, the prognosis is good, albeit, sequelae can occur. Case-fatality rate in different parts of the world ranging from less than 5% to 30%.Keywords: leptospirosis, diagnosis,Abstrak: Leptospirosis merupakan penyakit zoonosis yang umumnya timbul saat banjir dan umumnya ditularkan melalui kencing tikus. Indonesia merupakan negara dengan risiko sedang penularan leptospirosis. Leptospirosis memiliki manifestasi luas dari self limited hingga sakit berat. Pemeriksaan baku emas leptospirosis ialah dengan microscopic agglutination test. Diagnosis dibagi atas suspek, probable, dan konfirmasi. Terapi diberikan medikamentosa dengan antibiotik dan suportif. Prognosis umumnya baik namun bisa juga terjadi gejala sisa. Tingkat fatalitas kasus di berbagai belahan dunia berkisar <5%-30%.Kata kunci: leptospirosis, diagnosis

scholarly journals Sero-prevalence of Leptospira spp. in Household and Stray Dogs by Microscopic Agglutination Test

2020 ◽  
Vol 1 (1) ◽  
pp. 3-6
Author(s):  
Ali Shabestari Asl ◽  
Mohammad Bafandehzendeh ◽  
Gholamreza Abdollahpoor
Keyword(s):  
Zoonotic Disease ◽  
Climatic Conditions ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Human Beings ◽  
Serum Samples ◽  
Stray Dogs ◽  
Growth And Survival ◽  
Leptospira Serovars ◽  
Micro Organisms

Background: Leptospirosis is a zoonotic disease of worldwide significance in human beings and many animals. This zoonotic disease causes infection in dogs and other pet animals and these animals can influence human’s health. Given the unknown prevalence of leptospirosis in dogs in our area, this study was performed to investigate the sero-epidemiology of leptospirosis and evaluate the seroprevalence of Leptospira serovars in both household and stray dogs by microscopic agglutination test (MAT). Methods: Serum samples of 90 dogs in two household and stray groups (45 dogs for each group) were collected. Specimens were examined by MAT (in accordance with the procedure recommended by WHO) against antigens of serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona, Hardjo, Autumnalis, and Ballum. Results: In total, 6.7% of (n=6) dogs (1 stray and 5 household dogs) had a serum titer of 1:100 or more against Leptospira serovars. Dominant serovars were Canicola (8.9%), Grippotyphosa (2.2%), and Pomona (2.2%), respectively. The antibody titer against more than one serovar was observed only in one dog. The sero-prevalence was 11.1% in household dogs and 2.2% in stray dogs. Since 4 pet dogs had been vaccinated recently, it seems that the actual percentage of infection in household dogs must be just 2.2% and actual infection in dogs including stray dogs is approximately 4.4% in Tabriz. Conclusion: Fortunately, the rate of infection in Tabriz is low and this can be due to special geographical and climatic conditions, regular and annual vaccination of household dogs against this disease, and lack of suitable conditions for the growth and survival of such micro-organisms in the environment

scholarly journals Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1082
Author(s):  
Sujit Kumar Behera ◽  
Thankappan Sabarinath ◽  
Prasanta Kumar K. Mishra ◽  
Yosef Deneke ◽  
Ashok Kumar ◽  
...  
Keyword(s):  
B Cell ◽  
Animal Health ◽  
Three Dimensional ◽  
Agglutination Test ◽  
Dimensional Structure ◽  
Dipstick Test ◽  
Mhc I ◽  
Three Dimensional Structure ◽  
Bioinformatics Tools ◽  
Dot Elisa

Leptospirosis is responsible for hampering the productivity of swine husbandry worldwide. The aim of this study was to assess the efficacy of bioinformatics tools in predicting the three-dimensional structure and immunogenicity of recombinant LigBCon1-5 (rLigBCon1-5) antigen. A battery of bioinformatics tools such as I-TASSER, ProSA and SAVES v6.0 were used for the prediction and assessment of the predicted structure of rLigBCon1-5 antigen. Bepipred-2.0, DiscoTope v2.0 and ElliPro servers were used to predict linear and conformational epitopes while T-cell epitopes were predicted using NetMHCpan 4.1 and IEDB recommended 2.22 method for MHC Class I and II peptides respectively. The results obtained using various in silico methods were then compared with wet lab experiments comprising of both primary (IgG Dot ELISA Dipstick test) and secondary-binding assays (Latex Agglutination Test [LAT]) to screen 1153 porcine serum samples. The three-dimensional structure of rLigA/BCon1-5 protein as predicted by I-TASSER was found to be reliable by Ramachandran Plot and ProSA. The ElliPro server suggested 10 and three potential linear and conformational B-cell-epitopes, respectively, on the peptide backbone of the rLigA/BCon1-5 protein. The DiscoTope prediction server suggested 47 amino acid residues to be part of B-cell antigen. Ten of the most efficient peptides for MHC-I and II grooves were predicted by NetMHCpan 4.1 and IEDB recommended 2.22 method, respectively. Of these, three peptides can serve dual functions as it can fit both MHC I and II grooves, thereby eliciting both humoral-and cell-mediated immune responses. The prediction of these computational approaches proved to be reliable since rLigBCon1-5 antigen-based IgG Dot ELISA Dipstick test and LAT gave results in concordance to gold standard test, the Microscopic Agglutination Test (MAT), for serodiagnosis of leptospirosis. Both the IgG Dot ELISA Dipstick test and LAT were serodiagnostic assays ideally suited for peripheral level of animal health care system as “point of care” tests for the detection of porcine leptospirosis.

scholarly journals THE PERSISTENCE OF LEPTOSPIRAL AGGLUTININS TITERS IN HUMAN SERA DIAGNOSED BY THE MICROSCOPIC AGGLUTINATION TEST

1998 ◽  
Vol 40 (3) ◽  
pp. 183-184 ◽  
Author(s):  
Eliete C. ROMERO ◽  
Cynthia R. CALY ◽  
Paulo H. YASUDA
Keyword(s):  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Human Sera

The persistence of agglutinins detected by MAT has created some problems to the interpretation of the results. The aim of this study was to examine the data of serology from 70 patients with serologically confirmed diagnosis of leptospirosis by during 3-13 months after being affected with leptospires in order to elucidate the interpretation of the persistence of agglutinins detected by MAT. Sixty-one patients sera (87.14%) had titers equal or greater than 800. Of these, two individuals maintained titers of 800 thirteen months after the onset. This study showed that only one sample of sera with high titers is not reliable to determine the time at which infection occurred.

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