Diagnose of Mycobacterium bovis Using Interferon-gamma (IFN- and #947;) Assay and Post Mortem in Indian cattle (Bos indicus)

2017 ◽  
pp. 1
Author(s):  
Shubhra Shukla ◽  
Anuj Chauhan ◽  
Sarvjeet Kumar ◽  
Rajveer Maurya ◽  
Sudesh Rathaor ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Bos Indicus ◽  
Post Mortem ◽  
Indian Cattle

scholarly journals Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Charlene Clarke ◽  
Katrin Smith ◽  
Samantha J. Goldswain ◽  
Christopher Helm ◽  
David V. Cooper ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Human Health Risk ◽  
Molecular Transport ◽  
Sampling Technique ◽  
High Specificity ◽  
Negative Control ◽  
Post Mortem ◽  
Tissue Samples ◽  
Transport Medium ◽  
Tissue Homogenates

AbstractMycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transport Medium (MTM) has been shown to effectively inactivate infectious organisms, making it a safe method for handling infectious samples. This study investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues. Potentially infected tissues from bTB test-positive buffaloes were swabbed at post-mortem examination and stored in PrimeStore MTM at ambient temperature until Xpert MTB/RIF Ultra testing was performed. Additionally, tissue samples were frozen and transported before homogenisation for culture and Ultra testing. Oral swabs were collected from M. bovis-unexposed buffaloes as a negative control cohort. Mycobacterium tuberculosis complex (MTBC) DNA was detected by Ultra in 13/16 tissue swabs and 9/16 matched tissue homogenates from culture-confirmed M. bovis-positive buffalo tissues. MTBC DNA was not detected in swabs from M. bovis-unexposed animals, showing the potentially high specificity of Ultra with PrimeStore swabs. PrimeStore MTM sample processing, in combination with the Ultra assay, has the potential to provide a safe, rapid post-mortem screening test for M. bovis in buffaloes.

scholarly journals Improvement of bovine tuberculosis diagnosis

2021 ◽  
pp. 261-265
Author(s):  
M. O. Baratov
Keyword(s):  
Red Blood Cells ◽  
Mycobacterium Bovis ◽  
Blood Cells ◽  
Bovine Tuberculosis ◽  
Intradermal Test ◽  
Mycobacterium Fortuitum ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Antibody Titers ◽  
Specific Reactions

Detection of animals with non-specific reactions to tuberculin is one of the major problems in bovine tuberculosis (TB) diagnosis. There is a need to find and improve methods for detection of the sensitization causes. This paper presents the results of comparative studies of different ways to stabilize red blood cells in order to obtain diagnosticums for indirect hemagglutination (IHA) test. The article describes the stages of red blood cells stabilization and sensitization and demonstrates the diagnostic significance of Fili stabilization method using formaldehyde as a fixative. The highest antibody titers (1:3000 and 1:4000) were received in hyperimmune sera of rabbits immunized with Mycobacterium bovis using a homologous diagnosticum. Practical importance of the sensitins homologous to the infection is shown during testing of 1,911 serum samples collected from animals of different categories (diseased; healthy and reacting to tuberculin; healthy and not reacting to tuberculin) with IHA test using diagnosticums produced from Mycobacterium bovis and Mycobacterium fortuitum. Based on the positive results of the IHA test, TB was diagnosed in 87.5% of animals originating from an infected farm during post-mortem examination. The results of the IHA test agreed with those of the intradermal tuberculin test in 37.7% of cases. Diagnostic antibody titers were found in 206 TB infected animals with no reaction to the intradermal test. However, the post-mortem examination revealed TB changes in internal organs. The obtained data suggest a possibility to use the IHA test to detect TB infected animals with non-specific reactions to tuberculin.

scholarly journals Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
María Ángeles Risalde ◽  
Jobin Thomas ◽  
Iker Sevilla ◽  
Miriam Serrano ◽  
Jose Antonio Ortíz ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Red Deer ◽  
Gamma Assay

scholarly journals Bovine tuberculosis in one cattle herd in Ibadan in Nigeria

2012 ◽  
Vol 49 (No. 11) ◽  
pp. 406-412 ◽  
Author(s):  
S. I B Cadmus ◽  
N. N Atsanda ◽  
S. O Oni ◽  
E. E U Akang
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Molecular Typing ◽  
Human Population ◽  
Tuberculin Test ◽  
Cattle Herd ◽  
Post Mortem ◽  
Risk Of Infection ◽  
Cultural Isolation ◽  
Male Animal

Bovine tuberculosis was investigated in one private herd with 171 cattle after five cases were suspected to be tuberculous following post mortem examination. Using the intradermal comparative cervical tuberculin test 18 (10.5%) animals (ages from 2 to 12 years) were positive: 11 animals of N’dama breed and seven animals of White Fulani (i.e. Bunaji) breed; 17 female and one male animal. In all 11 randomly selected positive reactors, a spectrum of tuberculous lesions affecting the lungs, spleen, heart, liver, and the lymph nodes were observed. All the smear samples obtained were positive for acid-fast bacilli; cultural isolation confirmed the growth of mycobacteria on pyruvate-enriched Loewenstein-Jensen medium, which were identified by molecular typing to be Mycobacterium bovis. This study demonstrates widespread infection in this cattle herd and potential risk of infection for the human population with M. bovis.

Complete mitogenome reveals genetic divergence and phylogenetic relationships among Indian cattle (Bos indicus) breeds

2018 ◽  
Vol 30 (3) ◽  
pp. 219-232 ◽  
Author(s):  
R. Kumar Pramod ◽  
Dinesh Velayutham ◽  
Sajesh P. K. ◽  
Beena P. S. ◽  
Anil Zachariah ◽  
...  
Keyword(s):  
Genetic Divergence ◽  
Phylogenetic Relationships ◽  
Bos Indicus ◽  
Complete Mitogenome ◽  
Indian Cattle

scholarly journals Physicochemical properties of meat from Bos taurus and Bos indicus

2011 ◽  
Vol 40 (6) ◽  
pp. 1250-1259 ◽  
Author(s):  
Maria Cristina Bressan ◽  
Erika Cristina Rodrigues ◽  
Lizandra Vercezi Rossato ◽  
Eduardo Mendes Ramos ◽  
Luis Telo da Gama
Keyword(s):  
Physicochemical Properties ◽  
Meat Quality ◽  
Shear Force ◽  
Positive Relationship ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Post Mortem ◽  
Fresh Meat ◽  
Lower Shear ◽  
Before And After

The objective of this study was to characterize meat quality in Bos taurus and Bos indicus and to determine the influence of finishing system and genetic group on the physicochemical properties of the longissimus thoracis at 24 hours and 10 days post mortem (1ºC). The sample included 160 bulls of the B. taurus (n=75) and B. indicus (n=85) groups, finished either on pasture (n=46) or with grain supplementation (n=114), slaughtered at a carcass weight of 270 to 300 kg. Pasture-finished animals had higher pH, lower red content and a fat content 2.5 times lower than those finished with supplementation. Meat from supplement-finished animals had lower shear force in comparison to that from animals finished on pasture, with means of 7.7 and 8.5 kg at 24 hours, and of 5.5 and 5.9 kg at 10 days, respectively. Samples of B. taurus and B. indicus were similar in moisture and protein, but B. taurus presented higher means for ash and lower means for fat. Aged samples of B. indicus finished on pasture showed lower values for lightness and yellowness. Overall, lightness and yellow content increased and red content decreased with 10-day ageing. Means for shear force were lower in B. taurus than in B. indicus, with differences of 1.4 kg in fresh meat and 0.6 kg in aged samples. The reduction in shear force with ageing was more pronounced in samples with higher initial shear force, in spite of the positive relationship between shear force before and after ageing.

scholarly journals Tuberculin Skin Testing Boosts Interferon Gamma Responses to DIVA Reagents in Mycobacterium bovis-Infected Cattle

2017 ◽  
Vol 24 (5) ◽  
Author(s):  
Gareth J. Jones ◽  
Mick Coad ◽  
Bhagwati Khatri ◽  
Javier Bezos ◽  
Natalie A. Parlane ◽  
...  
Keyword(s):  
Skin Test ◽  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Skin Testing ◽  
Interferon Response ◽  
Tuberculin Skin Testing ◽  
Test Scenario ◽  
Positive Skin ◽  
Content Type ◽  
Bcg Vaccination

ABSTRACT Mycobacterium bovis BCG vaccination sensitizes cattle to bovine tuberculin, which compromises the use of the current bovine tuberculosis (TB) surveillance tests. Although the performance of a blood test (that utilizes antigens expressed by Mycobacterium bovis but not by BCG) capable of discriminating infected from vaccinated animals (DIVA interferon gamma test [DIT]) has been evaluated in naturally infected TB field reactors, there is a need to perform similar analysis in a BCG-vaccinated M. bovis-infected population. Furthermore, we explored different scenarios under which a DIT may be implemented alongside BCG vaccination: (i) serial testing to resolve potential false-positive skin test results or (ii) a standalone test to replace the single intradermal comparative cervical tuberculin (SICCT) skin test. Our results demonstrated significantly better relative test sensitivity when the DIT was evaluated in a serial test scenario. Direct comparison of pre- and post-skin test blood samples revealed that the SICCT test induced significant boosting of the gamma interferon response in M. bovis-infected animals to both the ESAT-6–CFP-10 and Rv3615c peptide cocktails that comprise the DIT, which persisted for the ESAT-6–CFP-10 reagent for at least 14 days. Importantly, no similar boosting effects were observed in noninfected BCG vaccinates, suggesting that DIVA blood testing after a recent skin test would have minimal impact on test specificity.

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