Abstract
Background: Saline soils severely affect plant growth. Associations between endophytes and plants are known to significantly alter plant metabolism. This study reports the effects of a fungal endophyte species (Botryosphaeria ramosa ssp.) on osmoregulation, antioxidant enzymes, and the regulation of endogenous plant hormones in Casuarina equisetifolia under NaCl stress. C. equisetifolia plants, with and without B. ramosa ssp. colonization, were subjected to different levels of NaCl stress (0%, 5%, 10%, and 15%) for different amounts of time (0 d, 20 d, 40 d, and 60 d). Results: Antioxidant enzymes, phytohormones, and nutritive elements in the leaves and roots were determined. The results showed that colonization of the roots by B. ramosa ssp. improved the growth rate and dry weight of salt-stressed plants. Moreover, B. ramosa ssp. colonization increased the activities of superoxide dismutase, catalase, and peroxide but decreased the hydrogen peroxide content in the branches of C. equisetifolia under salt stress. Meanwhile, compared with non-colonized plants, endophyte colonization reduced the abscisic acid and proline contents but increased the contents of auxin, zeatin, and gibberellins. Importantly, the nutrient elements in the roots and branches of colonized plants were significantly different from those in the roots and branches of non-colonized plants under saline conditions. Conclusions: The results of this study showed that B. ramosa ssp. colonization can enhance the salt tolerance of C. equisetifolia. by improving the antioxidant enzyme content, regulating the phytohormones, and adjusting proline accumulation under NaCl stress.