Mitigative Effects of Alpha-lipoic Acid on the Toxicity of Dimethoate in Male Rats

The present study was aimed to investigate the role of alpha-lipoic acid ( ALA) as an antioxidant against in ameliorating histological disorders of pituitary- testicular axis- induced by hydrogen peroxide (H2O2) in adult Wistar rats. Forty adult male rats were randomly divided into four equal groups (10 rats /group) and were handled daily as follows for 56 days: Control group ( C) were intubated distilled water and received ordinary tap water; group T1 were intubated 60mg/kg B.W of ALA and received ordinary tap water; group T2 were administered H2O2 in tap water at a concentration of 0.05%, while group T3 were intubated 60mg/kg B.W of ALA and received ordinary tap water containing 0.05% H2O2. At the end of the experiment, body weights were recorded, then pituitary and testes were excised for histopathological study and testicular weight was recorded too. Rats administered H2O2 showed a significant decrease in testes weight to body weight ratio accompanied with major histopathological changes of the testes in comparison with other groups including; a significant decrease in the diameter of seminiferous tubules, high of germinal epithelial cell and degenerative changes with incomplete spermatogenesis. Besides, a significant decrease in the number of Leydig's cells in comparison with other experimental groups. Furthermore, pituitary gland of group T2 manifested a severe histological alteration in architecture characterized by atrophy with marked necrotic and degenerative changes. Whereas, rats administered ALA (group T3) shows an improvement of histological changes of pituitary and testicular tissues induced by hydrogen peroxide. In conclusion, the results indicated that alpha-lipoic acid mitigated pituitary-testicular dysfunctions induced by H2O2 through its antioxidant effects via scavenging free radicals.

Download Full-text

Chronic Maternal Tobacco Smoke Exposure and/or Alpha-Lipoic Acid Treatment Causes Long-Term Deterioration of Testis and Sexual Behavior in Adult Male Rats

Journal of Sexual Medicine ◽

10.1016/j.jsxm.2020.07.002 ◽

2020 ◽

Vol 17 (10) ◽

pp. 1835-1847

Author(s):

Ahmet Yardimci ◽

Ramazan Fazil Akkoc ◽

Ahmet Tektemur ◽

Nazife Ulker ◽

Nalan Kaya Tektemur ◽

...

Keyword(s):

Sexual Behavior ◽

Adult Male ◽

Lipoic Acid ◽

Tobacco Smoke ◽

Acid Treatment ◽

Smoke Exposure ◽

Tobacco Smoke Exposure ◽

Male Rats ◽

Alpha Lipoic Acid

Download Full-text

Mitigative effects of Alpha-lipoic acid for the toxicity of Dimethoate in male rats

10.1101/527283 ◽

2019 ◽

Cited By ~ 1

Author(s):

Hani M. Abdelsalam

Keyword(s):

Lipid Profile ◽

Lipoic Acid ◽

Histopathological Examination ◽

Liver Function Tests ◽

Male Rats ◽

Albino Rats ◽

Alpha Lipoic Acid ◽

Negative Effects ◽

Negative Effect ◽

Liver And Kidney

AbstractBackgroundOrganophosphates are widely used by human nowadays, but these compounds have tremendous negative effect on the man health. So this study aims to use of Alpha-lipoic acid (ALA) to alleviates the negative effects of Dimethoate (DM).MethodsThis study is designed as follows, Thirty adult male Wistar albino rats were utilized, further subdivided into control, DM and DM+ALA groups. Liver and renal cortex sections from all groups were processed for histopathological examination, biochemical estimation of liver function tests, serum Urea, Creatinine, BUN, testosterone and lipid profile were performed.ResultsThis study clarified the improvement effects of ALA against the negative effects of DM where ALA caused a significant recovery of the hepatic (ALT, AST, ALP and total protein) and renal functions by normalizing them in DM + ALA group and to some extent improvement of lipid profile and testosterone levels. Also, ALA restored normal hepatic and renal histomorphology.ConclusionIt is concluded that ALA therapy can ameliorate the negative effects of DM that affect the vital organs as the liver and kidney. Also ALA can reduce the occurrence of atherogensis by reducing the levels of bad cholesterol in the blood. ALA boosts the levels of testosterone so it augments the male sexual characters.

Download Full-text

The possible Protective role of alpha-lipoic acid on nanosilver particle-induced hepatotoxicity in male rats

Biochemistry Letters ◽

10.21608/blj.2019.47569 ◽

2019 ◽

Vol 14 (1) ◽

pp. 85-97

Author(s):

Eman M. Abd Elmaksoud ◽

Nabil M.Taha ◽

Mohamed A. Lebda ◽

Maher A. Kamel

Keyword(s):

Lipoic Acid ◽

Protective Role ◽

Male Rats ◽

Alpha Lipoic Acid

Download Full-text

Efek asam alfa lipoat terhadap insulitis pada tikus diabetes melitus tipe 2

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.31701 ◽

2019 ◽

Vol 16 (2) ◽

pp. 58

Author(s):

Ismawati Ismawati ◽

Mukhyarjon Mukhyarjon ◽

Ilhami Romus ◽

Dinda Sonia

Keyword(s):

Type 2 Diabetes ◽

Lipoic Acid ◽

Male Rats ◽

Measured Parameter ◽

Alpha Lipoic Acid ◽

Β Cell ◽

Diabetes Group ◽

Significant Difference ◽

Type 2 Diabetic

Effect of alpha lipoic acid on insulitis in type 2 diabetic ratBackground: The damaging of β cell causes hyperglycemia. Β cell damaged as insulitis happens because of the increase of free radical and the decrease of endogen antioxidant that caused oxidative stress.Objective: The goal of this research was to find out the effect of alpha lipoic acid (ALA) on pancreas Langerhans island’s histopathology in type 2 diabetic rats.Methods: This was an experimental laboratory study with post test only design. Fifteen adult male rats of Wistar strain were segregated into three groups (n=5) labeled as control, type 2 diabetes (DM), and DM+ALA. The experiment was designed for 3 weeks. The measured parameter was insulitis level on pancreas Langerhans island of groups labeled.Results: The statistical test result showed there was the significant difference between control and type 2 diabetes group (p=0,005), but there was no significant difference between DM and DM+ ALA group (p=0,549).Conclusions: Although not statistically significant, giving ALA 60 mg/kg body weight for 3 weeks decreased the degree of insulitis in diabetic rats.

Download Full-text

Simultaneous Effect of Resistance Training and Alpha Lipoic Acid on Beclin-1 Expression and Malondialdehyde Level of Fatty Liver Diabetic Male Rats

Gene Cell and Tissue ◽

10.5812/gct.113497 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Fatemeh Jafary Nia ◽

Bahareh Yazdanparast Chaharmahali ◽

Farah Nameni

Keyword(s):

Fatty Liver ◽

Strength Training ◽

Training Program ◽

Lipoic Acid ◽

Diabetic Control ◽

Diabetic Rats ◽

Laboratory Method ◽

Male Rats ◽

Alpha Lipoic Acid ◽

Male Wistar Rats

Background: Autophagy is a genetically and evolutionarily conserved programmed process that destroys long-lived cellular proteins and organelles. Objectives: The aim of this study was to evaluate the effect of eight weeks of strength training with alpha-lipoic acid supplementation on Becline-1 and malondialdehyde concentrations in elderly diabetic rats. Methods: In this experimental study, 35 old male Wistar rats were randomly divided into five groups (healthy control, diabetic, diabetic + strength training, diabetic + supplement, diabetic + strength training + supplement). At first, the rats became diabetic and had fatty liver. Then strength training program was performed in eight weeks. Alpha-lipoic acid supplement was also injected three days a week. At the end of the training period, tissues were removed, and the expression of Becline-1 was measured by RT-PCR, and malondialdehyde concentration was measured by TBA laboratory method. For data analysis, a one-way analysis variance test was used for between-groups comparison (P < 0.05). Results: The results showed that strength training program and in combination with alpha-lipoic acid (ALA) supplementation significantly reduced Becline-1 (P = 0.001) and malondialdehyde compared to the healthy and diabetic control groups (P = 0.000). Conclusions: Strength exercise combined with ALA supplementation has a significant role in reducing the rate of autophagy and can reduce fatty liver in elderly type 2 diabetic rats.

Download Full-text

Antioxidant and anti-inflammatory properties of alpha lipoic acid protect against valproic acid induced liver injury

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2019-0456 ◽

2020 ◽

Author(s):

Marwa Abdeltawab Mohammed ◽

Doaa Mostafa Gharib ◽

Hoda Ramadan Reyad ◽

Alaa Aboud Mohamed ◽

Fadwa A Elroby ◽

...

Keyword(s):

Valproic Acid ◽

Adverse Effects ◽

Lipoic Acid ◽

Liver Toxicity ◽

Male Rats ◽

Control Group ◽

Alpha Lipoic Acid ◽

Group 4 ◽

Anti Inflammatory ◽

Group 2

Valproic acid (VPA) is one of the most used anti-epileptic drugs inspite of its many adverse effects as anemia, leucopenia, thrombocytopenia, and liver toxicity. The hepatoprotective effect of alpha-lipoic acid (ALA) was confirmed. Aim of the study: The aim of this study was to detect the protective effect of ALA against the adverse effects of VPA. Materials& Methods: Thirty white albino Wistar male rats were divided into 4 groups. Group (1) was the control group; Group (2) included rats that received ALA (100mg/kg/day) orally for 14 days; Group (3) and Group (4) included rats that received VPA (500 mg/kg/day) for 15 days intraperitoneal, but group 4 rats received ALA (100mg/kg/day) orally for 14 days prior to VPA. Blood samples were collected and livers were excised from rats for colorimetric analysis and rt-PCR. Results: Rats that received VPA showed leucopenia, thrombocytopenia, a significant decrease of SOD, glutathione, Nrf2, and Sirt1, besides a significant increase of MDA and TNF alpha. Prior treatment with ALA prevented all these results. Conclusion: ALA protected against VPA-induced liver damage and hematological disturbance via anti-oxidant and anti-inflammatory properties.

Download Full-text