scholarly journals Role of Alpha Lipoic Acid in Ameliorating the Histological Alterations of Pituitary-Testicular Axis –induced by Hydrogen Peroxide in Rats

2020 ◽  
Vol 13 (2) ◽  
pp. 125-136
Author(s):  
Mohammad Alwan ◽  
Baraa Al-Okialy
Keyword(s):  
Hydrogen Peroxide ◽  
Lipoic Acid ◽  
Tap Water ◽  
Degenerative Changes ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Alpha Lipoic Acid ◽  
Water Group ◽  
Testicular Weight

The present study was aimed to investigate the role of alpha-lipoic acid ( ALA) as an antioxidant against in ameliorating histological disorders of pituitary- testicular axis- induced by hydrogen peroxide (H2O2) in adult Wistar rats. Forty adult male rats were randomly divided into four equal groups (10 rats /group) and were handled daily as follows for 56 days: Control group ( C) were intubated distilled water and received ordinary tap water; group T1 were intubated 60mg/kg B.W of ALA and received ordinary tap water; group T2 were administered H2O2 in tap water at a concentration of 0.05%, while group T3 were intubated 60mg/kg B.W of ALA and received ordinary tap water containing 0.05% H2O2. At the end of the experiment, body weights were recorded, then pituitary and testes were excised for histopathological study and testicular weight was recorded too. Rats administered H2O2 showed a significant decrease in testes weight to body weight ratio accompanied with major histopathological changes of the testes in comparison with other groups including; a significant decrease in the diameter of seminiferous tubules, high of germinal epithelial cell and degenerative changes with incomplete spermatogenesis. Besides, a significant decrease in the number of Leydig's cells in comparison with other experimental groups. Furthermore, pituitary gland of group T2 manifested a severe histological alteration in architecture characterized by atrophy with marked necrotic and degenerative changes. Whereas, rats administered ALA (group T3) shows an improvement of histological changes of pituitary and testicular tissues induced by hydrogen peroxide. In conclusion, the results indicated that alpha-lipoic acid mitigated pituitary-testicular dysfunctions induced by H2O2 through its antioxidant effects via scavenging free radicals.

scholarly journals Role of Alpha Lipoic Acid in Oxidant /Antioxidant Status and Gene Expression of Glutathione Reductase in Hydrogen Peroxide Exposed Rats: (Part -2)

2019 ◽  
Vol 42 (2) ◽  
pp. 50-57
Author(s):  
Baraa Najim Al-Okialy
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Hydrogen Peroxide ◽  
Glutathione Reductase ◽  
Lipoic Acid ◽  
Antioxidant Status ◽  
Tap Water ◽  
Alpha Lipoic Acid ◽  
Water Group

     This study was designated to evaluate the protective role of alpha lipoic acid against oxidative stress resulted by hydrogen peroxide on some oxidants/ antioxidants  parameters and gene expression of glutathione peroxidase in  adult Wistar rats. Forty  adult male rats were randomly divided into four equal groups (10 rats /group) and were handled daily as follows for  56 days : Control group were intubated distal water and received ordinary tap water ; group T1 were intubated  60mg/kg B.W of alpha lipoic acid and received ordinary tap water ; group T2 were received  hydrogen peroxide in tap water at concentration of 0.5% , while group T3  were intubated  60mg/kg B.W of alpha lipoic acid and received ordinary tap water containing 0.5% hydrogen peroxide. Fasting blood samples were collected at 0, 28 and 56 days of experimental periods for measurement of serum peroxynitrite and malondialdehyde concentrations, as well catalase activity. Furthermore, gene expression of glutathione reductase in liver was investigated. Administration of 0.5% hydrogen peroxide in drinking water (group T2) manifested a significant elevation in serum peroxynitrite and malondialdehyde with significant decrease in catalase and Glutathione, concentrations. Also, a significant decrease in gene expression of glutathione reductase was observed as compared to other treated groups. Nevertheless, rats in group T3 shows a significantly improvement in oxidant /antioxidant status with increase in folds changes of gene expression of glutathione reductase as compared to control and T2. In conclusion, supplementation of alpha lipoic acid to rats significantly reduced oxidative stress –induced by hydrogen peroxide and caused improvement of gene expression of glutathione reductase in liver via its antioxidant properties.  

scholarly journals Role of alcoholic extract of Roket (Eruca sativa) leaves on male reproduction of experimentally induced-oxidative stressed rats

2015 ◽  
Vol 39 (2) ◽  
pp. 47-54
Author(s):  
Baraa Najim AL-Okaily
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Tap Water ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Male Reproduction ◽  
Seminiferous Tubules ◽  
Eruca Sativa ◽  
Histological Sections

     This study conducted to find out the protective role of ethanolic extract of Eruca sativa leaves against the deleterious effect of hydrogen peroxide on some aspect of male reproduction in adult rats. Forty adult male rats were randomly assigned into four equal groups as follows: control group received tap water (untreated); group T1 were received tap water containing 0.5% H2O2; group T2 were received tap water containing 0.5% H2O2 plus administration of 300 mg/kg. B.W. ethanolic extract of Eruca sativa leaves and group T3 administration ethanolic extract of Eruca sativa leaves only at the same dose of group T2. All treatments continued for 60 days. At the end of the experiment, samples of tests and epididymis tissues were taken to prepare histological sections for                                                                                                                                                                                                       measurement the diameter of seminiferous tubules, thickness of epithelial cells of seminiferous tubules and histological examination of testes and epididymis. The results in Group T1 showed a significant decrease in the diameter and thickness of epithelial cells of seminiferous tubules, but these parameters clarified a significant increase in T2 and T3 groups as compared with T1 group. Histological sections of testis and epididymis in group T1 revealed incomplete spermatogenesis, cell debris, vacculation of Sertoli cells and view sperms in the lumen of seminiferous tubules and epididymis. Besides, normal obvious histological architecture of seminiferous tubules and epididymis with complete spermatogenesis were shown in sections of testis and epididymis of T2 and T3 groups' as compared to T1. In conclusion, hydrogen peroxide may impair spermatogenesis, furthermore, the results confirm the protective role of E. sativa leaves extract against oxidative stress induced by H2O2 in rats.

scholarly journals The possible Protective role of alpha-lipoic acid on nanosilver particle-induced hepatotoxicity in male rats

2019 ◽  
Vol 14 (1) ◽  
pp. 85-97
Author(s):  
Eman M. Abd Elmaksoud ◽  
Nabil M.Taha ◽  
Mohamed A. Lebda ◽  
Maher A. Kamel
Keyword(s):  
Lipoic Acid ◽  
Protective Role ◽  
Male Rats ◽  
Alpha Lipoic Acid

Role of testicular macrophages in the response of Leydig cells to gonadotrophins in young hypophysectomized rats

1995 ◽  
Vol 147 (3) ◽  
pp. 463-471 ◽  
Author(s):  
F Gaytan ◽  
C Bellido ◽  
C Morales ◽  
N van Rooijen ◽  
E Aguilar
Keyword(s):  
Leydig Cells ◽  
Male Rats ◽  
Ventral Prostate ◽  
Seminiferous Tubules ◽  
Left Testis ◽  
Cross Sectional ◽  
Testicular Weight ◽  
Hypophysectomized Rats ◽  
Testicular Macrophages

Abstract The Leydig cells of young hypophysectomized rats are highly sensitive to the stimulatory effects of exogenous pituitary hormones. The aim of this study was to analyse the role of testicular macrophages in the response of Leydig cells to different hormones. Male rats were hypophysectomized at 28 days of age and 10 days later they were injected intratesticularly with dichloromethylene diphosphonate-containing liposomes (right testis) to deplete testicular macrophages, and with 0·9% NaCl (left testis). One week later, the animals were treated daily with 1 IU rat GH (rGH)/rat, 5 IU recombinant human FSH (recFSH)/rat, 10 IU human chorionic gonadotrophin (hCG)/rat, or vehicle for 7 days. The animals were killed on the day after the last injection. The animals treated with rGH showed increased body weight and increased number and size of testicular macrophages in the left testes, but no significant effects on Leydig cells were found. Treatment with recFSH induced a significant increase in testicular weight and tubular diameter in both testes. In the left testes, the number and size of macrophages were increased; the number of Leydig cells was not changed, although they showed a significantly increased cross-sectional area. This effect was abolished in the right (macrophage-depleted) testes. However, the effect of recFSH on the growth of the seminiferous tubules was not modified by the absence of macrophages. Rats treated with hCG showed increased testicular weight and serum testosterone levels, as well as an increased weight of the ventral prostate. In the left testes, the number and size of both macrophages and Leydig cells were increased. Otherwise, the number of Leydig cells was unchanged in the absence of macrophages, whereas the increase in the size of Leydig cells was partially abolished. These data indicate that testicular macrophages are needed for the response of Leydig cells to gonadotrophin treatment. Journal of Endocrinology (1995) 147, 463–471

scholarly journals The role of oxidative stress and effect of alpha-lipoic acid in reexpansion pulmonary edema – an experimental study

2010 ◽  
Vol 6 ◽  
pp. 848-853 ◽  
Author(s):  
Seyfettin Gumus ◽  
Orhan Yucel ◽  
Mehmet Gamsizkan ◽  
Ayse Eken ◽  
Omer Deniz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Study ◽  
Pulmonary Edema ◽  
Lipoic Acid ◽  
Alpha Lipoic Acid ◽  
Reexpansion Pulmonary Edema

Involvement of physiological prolactin levels in growth and prolactin receptor content of prostate glands and testes in developing male rats

1992 ◽  
Vol 132 (3) ◽  
pp. 449-459 ◽  
Author(s):  
B. Pérez-Villamil ◽  
E. Bordiú ◽  
M. Puente-Cueva
Keyword(s):  
Body Weight ◽  
Male Rats ◽  
Ventral Prostate ◽  
Serum Prolactin ◽  
Plasma Prolactin ◽  
Stages Of Development ◽  
Continuous Increase ◽  
Testosterone Levels ◽  
Testicular Weight

ABSTRACT We have investigated the role of physiological prolactin levels in the development of prepubertal male rats. Prolactin GH and testosterone levels, as well as body, ventral prostate and testicular weight, have been analysed in both control and bromocriptine-treated rats between 21 and 60 days of life. Furthermore the role of prolactin in the regulation of its own receptors has also been studied during the same period. In control rats, prolactin levels showed a prepubertal peak of secretion at 25 days of age. At this time GH and testosterone levels were low and did not show any significant variation. After this age, prolactin levels increased more gradually; determinations of GH showed great variation with low levels in most of the rats and very high values in the other animals; testosterone levels remained low until day 35 after which they increased. Simultaneously with the serum prolactin peak on day 25, a decrease in prolactin-binding capacity of ventral prostate glands, was observed and a maximum rate of body, prostate and testicular weight gain was obtained. Furthermore, in rats with pharmacologically suppressed serum prolactin levels (lower than 1 μg/l), prolactin binding to prostate glands as well as the weight of body, ventral prostate and testes were lower than in control animals. When results were expressed in mg prostate or testes/g body weight, testes from 25-day-old treated rats weighed significantly less than controls. The later stages of development, from days 25 to 60, were characterized by an initial decline in serum prolactin levels at 29 days of age which was followed by a continuous increase until adult values were reached. During this period, prostatic prolactin receptors which were at their lowest value at 33 days of age showed a gradual rise parallel with the observed increase in plasma prolactin levels. When testicular tissue was analysed, no changes in prolactin-binding sites caused by sexual maturation were observed. The present results indicate that physiological prolactin secretion has a specific effect on the normal increase in the prostate, testes and body weight and clearly is also implicated in the regulation of its prostatic receptors at the earlier stages of development. Journal of Endocrinology (1992) 132, 449–459

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