scholarly journals Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes

ADMET & DMPK ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 91
Author(s):  
Alejandro Palacios ◽  
Javier Barberón ◽  
Patricio Leaden ◽  
Pedro Zeinsteger

<p class="ADMETabstracttext">In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe<sup>2+</sup> (2.15 µM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4w6 and docosahexaenoic acid C22:6w3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.</p>

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1253
Author(s):  
Chae-Hyung Sun ◽  
Jae-Sung Lee ◽  
Jalil Ghassemi Nejad ◽  
Won-Seob Kim ◽  
Hong-Gu Lee

We evaluated the effects of a rumen-protected microencapsulated supplement from linseed oil (MO) on ruminal fluid, growth performance, meat quality, and fatty acid composition in Korean native steers. In an in vitro experiment, ruminal fluid was taken from two fistulated Holstein dairy cows. Different levels of MO (0%, 1%, 2%, 3%, and 4%) were added to the diet. In an in vivo experiment, eight steers (average body weight = 597.1 ± 50.26 kg; average age = 23.8 ± 0.12 months) were assigned to two dietary groups, no MO (control) and MO (3% MO supplementation on a DM basis), for 186 days. The in vitro study revealed that 3% MO is an optimal dose, as there were decreases in the neutral detergent fiber and acid detergent fiber digestibility at 48 h (p < 0.05). The in vivo study showed increases in the feed efficiency and average daily gain in the 3% MO group compared to the control group on days 1 to 90 (p < 0.05). Regarding meat quality, the shear force produced by the longissimus thoracis muscle in steers from the 3% MO group was lower than that produced by the control group (p < 0.05). Interestingly, in terms of the fatty acid profile, higher concentrations of C22:6n3 were demonstrated in the subcutaneous fat and higher concentrations of C18:3n3, C20:3n3, and C20:5n3 were found in the intramuscular fat from steers fed with 3% MO (p < 0.05). Our results indicate that supplementation with 3% MO supplements improves the growth performance and meat quality modulated by the omega-3 fatty acid content of meat in Korean native steers.


Author(s):  
Pelin Özlem Can ◽  
Gonca Kaşıkçı

In this study, rosemary and thyme oil extracts obtained using trout (Oncorhynchusmykiss) marinades of the chemical changes that occur during storage was investigated and effect on fatty acid composition of vegetable oil extracts used in the product are determined. Using three different formulations are made into fish fillets (M: 4% acetic acid-10% NaCl-0.1%, B: 4% acetic acid-10% NaCl-0.1% of rosemary oil extract, K: 4% acetic acid - 10% NaCl-%0.1 thymol oil extract) was marinated, after the maturation stage vacuum packaged at +4°C in the refrigerator conditions have been stored. Samples; 0 days 1, 2, 3, 4 and 5 at the end of the month chemical analysis (pH, TBA, peroxide value, TVB-N, free fatty acids, fatty acid composition) were conducted. Experimental samples were evaluated in term of TVB-N determined M group samples 28.9 mg/100 g, B group samples19.2 mg/100 g and K group samples 14.12 mg/100 g in the third month of storage. When the samples of M group were evaluated in terms of TBA number, it was 5.84 mg MDA / kg at the 3rd month of storage and statistical difference was found to be significant with the other groups. The control group (M group) samples analysed were not performed for sensory deterioration at 4 and 5 months. After marination, the control group and K group samples decreased EPA and DHA during storage period, it was found statistically significant difference. When the fatty acid values of the samples belonging to the group containing rosemary were examined, caproic, myristic and palmitic acid amounts increased after the marinate and difference statistically significant.


2006 ◽  
Vol 410 (1) ◽  
pp. 364-366
Author(s):  
V. A. Otellin ◽  
I. P. Grigor’ev ◽  
S. A. Zabelinskii ◽  
M. A. Chebotareva ◽  
A. I. Krivchenko ◽  
...  

2002 ◽  
Vol 50 (3) ◽  
pp. 357-364 ◽  
Author(s):  
Éva Szabó ◽  
R. Romvári ◽  
Hedvig Fébel ◽  

The present study was designed to investigate whether meat-type rabbits are able to perform treadmill running as a daily routine exercise, and if so, whether the exercise induces specific proportional changes in the fatty acid composition of their muscles. After a four-week training period 8-week-old rabbits were slaughtered and the total activity of plasma lactate dehydrogenase was measured, showing a significant difference between the exercised and control groups (429 ± 126 IU/l vs. 639 ± 203 IU/l). Furthermore the fatty acid composition of m. longissimus dorsi (MLD) and m. vastus lateralis (MVL) was determined by means of gas chromatography. Exercise increased the proportions of oleic acid (C18:1 n-9) in both MLD and MVL as compared to the control group. However, the level of stearic (C18:0) and arachidonic (C20:4 n-6) acids significantly decreased in the MVL after the exercise. Changes in the fatty acid profile resulting from the physically loaded condition were of the same tendency in both muscles, adding that the MVL might have been exposed to the exercise more intensively; alterations there occurred in a more pronounced manner. Based on the inference that the composition of membrane structure was also affected, these alterations may have important consequences on meat quality.


Lipids ◽  
2000 ◽  
Vol 35 (10) ◽  
pp. 1107-1115 ◽  
Author(s):  
Atsushi Ikemoto ◽  
Masayo Ohishi ◽  
Noriaki Hata ◽  
Yoshihisa Misawa ◽  
Yoichi Fujii ◽  
...  

2008 ◽  
Vol 88 (3) ◽  
pp. 475-483 ◽  
Author(s):  
Maziar Mohiti-Asli ◽  
Farid Shariatmadari ◽  
Houshang Lotfollahian ◽  
Mohamad Taghi Mazuji

A 7-wk trial was carried out to investigate the effect of vitamin E and inorganic and organic selenium added to hens' diet on quality and lipid stability of eggs during storage. One hundred forty-four Hy-Line W-36 hens (63-wk of age) were divided into six equal groups. Five groups received a basal diet supplemented with 0.4 mg kg-1 sodium selenite or selenium yeast, 200 mg kg-1 vitamin E or a combination of selenium and vitamin E; whereas the control group received no supplementation. Hen production was assessed daily and fresh egg quality parameters were determined every 2 wk. Eggs were stored under different conditions (4°C, 23–27°C or 31°C) for 14 d. Eggs were analyzed for quality characteristics, egg component weight, Malondialdehyde values as a secondary oxidation product and yolk fatty acid (FA) composition. The performance of the hens and egg weights were not affected either by the source of the selenium or by the vitamin E. The inclusion of selenium or vitamin E in the diet significantly increased their concentrations in the egg. The supplemented diets also improved egg quality, oxidative stability and fatty acid composition during storage. Key words: Vitamin E, selenium, lipid peroxidation, egg fatty acid composition, storage


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