Effect of a high amino acid diet on antioxidant barrier parameters of rat skin. Part 2

2019 ◽  
Vol 1 ◽  
pp. 70-75
Author(s):  
M. Knaś ◽  
M. Niczyporuk ◽  
K. Grądzka ◽  
H. Car
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Body Weight ◽  
Amino Acid ◽  
The Body ◽  
Total Protein Content ◽  
Standard Diet ◽  
Acid Diet ◽  
High Amino Acid ◽  
Amino Acid Diet

<b>Purpose:</b> The imbalance between the formation of reactive oxygen species and antioxidant capacity of the body is known as oxidative stress. Exposition of the skin to free radicals, the origin of the internal and external causes activation of multiple mechanisms to eliminate them and prevent in this way the development of oxidative stress. The aim of this experiment was examining what changes are taking place in the antioxidant barrier of unwounded healthy skin of rats, who are on a high amino acids diet for 7 and 14 days at administered doses of 0.3 and 0.5 g/kg body weight. <br/><b>Materials and Methods:</b> The study was performed on male Wistar rats divided into 5 groups: 1. control (standard feed), 2. high amino acid diet (WPC-80 80% whey protein) administered for 7 days at a dose of 0.3g/kg of body weight, 3. WPC-80 for 7 days at a dose of 0.5g/kg of body weight, 4. WPC-80 for 14 days at a dose of 0.3g/kg of body weight, 5. WPC-80 for 14 days at a dose of 0.5g/kg of body weight. The concentration of superoxide dismutase 2 and 3, the concentration of catalase specific activity of glutathione peroxidase, the concentration of glutathione and total protein content were determined. <br/><b>Results:</b> The supplementation of the standard diet by the preparation of WPC-80 administered in a dose 0.5 g/kg body weight for 14 days containing methionine and cysteine (essential amino acids involved in the formation of glutathione), significantly increases the concentration of reduced glutathione. <br/><b>Conclusions:</b> Enrichment of a standard diet with WPC-80 caused by the significant increases of non-enzymatic antioxidant.

Effect of a high amino acid diet on antioxidant barrier parameters of rat skin.

2019 ◽  
Vol 1 ◽  
pp. 45-50
Author(s):  
M. Niczyporuk ◽  
M. Knaś ◽  
K. Grądzka ◽  
H. Car
Keyword(s):  
Body Weight ◽  
Amino Acid ◽  
Oxidative Status ◽  
Standard Diet ◽  
Rat Skin ◽  
Acid Diet ◽  
High Amino Acid ◽  
Total Antioxidant ◽  
Total Oxidative Status ◽  
Amino Acid Diet

<b>Introduction:</b> Oxidative stress is largely responsible for numerous skin complications that occur in the course of various diseases as well as accelerated skin aging. A high amino acid diet, supplemented with whey protein concentrate (WPC), is well-balanced and has well-absorbing proteins, which are an ideal source of essential amino acids. <br/><b>Purpose:</b> To assess what changes will occur in the antioxidant barrier of unharmed skin of rats on a high amino acid diet. <br/><b>Materials and methods:</b> The study was conducted on sexually mature male Wistar rats (160-180g): 1. control (standard feed), 2. high amino acid diet (WPC-80 80% whey protein) administered for 7 days at a dose of 0.3g/kg of body weight, 3. WPC-80 for 7 days at a dose of 0.5g/kg of body weight, 4. WPC-80 for 14 days at a dose of 0.3g/kg of body weight, 5. WPC-80 for 14 days at a dose of 0.5g/kg of body weight. Total antioxidant capacity, total oxidative status and oxidative stress index were determined. <br/>Results:</b> Enrichment of a standard diet with WPC-80 did not affect the total oxidative status of undamaged healthy rat skin. This study shows that a diet rich in amino acids in rats caused an increase in total antioxidant capacity, but statistically significant values were obtained after 14 days of administering WPC at a dose of 0.5mg/kg of body weight. <br/><b>Conclusions:</b> Enrichment of a standard diet with WPC-80 strengthens the antioxidant barrier in unwounded healthy rat skin.

scholarly journals Studies on unidentified growth factors

1974 ◽  
Vol 31 (2) ◽  
pp. 179-184 ◽  
Author(s):  
J. Bunyan ◽  
Elspeth A. Murrell ◽  
M. A. Cawthorne ◽  
B. T. Redman
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Amino Acid ◽  
Soya Bean ◽  
Torula Yeast ◽  
Acid Diet ◽  
Organic Growth ◽  
Organic Factor ◽  
Amino Acid Diet ◽  
Low Activity

1. Chicks at 10 d of age were given diets containing all the nutrients known to be required, with L-amino acids in place of protein. Dietary supplements were added isonitrogenously.2. Ox liver (100 g/kg) greatly improved the chicks' growth rate, whereas torula yeast (50 g/kg) was inactive. The activity of fresh moist liver was considered to be due largely to an organic factor, because of the inactivity of water and the low activity of liver ash.3. The growth rates of chicks receiving diets based upon casein and isolated soya-bean protein were significantly improved by the inclusion of 100 g fresh ox liver/kg, but not of 50 g torula yeast/kg.4. These results show that chicks receiving an amino acid diet require an organic growth factor (Progressin) that differs from the yeast factor required by rats receiving an amino acid diet.

Effect of amino acid blend as alternative to antibiotics for growing pigs

2022 ◽  
Author(s):  
Maykelly da S Gomes ◽  
Alysson Saraiva ◽  
Dante T Valente Júnior ◽  
Leandro L de Oliveira ◽  
Amanda M Correia ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Control Diet ◽  
Growing Pigs ◽  
Daily Weight Gain ◽  
Feed Ratio ◽  
Antioxidant Power ◽  
Acid Diet ◽  
Amino Acid Diet ◽  
Intermediate Results

Abstract This study aimed to evaluate the effect of supplementing arginine (Arg) + glutamine (Gln) replacing antibiotics on performance, immune response, and antioxidant capacity of pigs in the growing phase. One hundred and fifty 63-d-old pigs with initial body weight (BW) of 25.0 ± 1.46 kg were distributed in a randomized block design, with three treatments and ten replicates. The three diets were control; antibiotic, control + 100 mg/kg tiamulin and 506 mg/kg oxytetracycline; amino acid, control + 10 g/kg Arg and 2 g/kg Gln. Dietary treatments were fed from 63 to 77 d. Following the treatment period, all pigs were fed the control diet from 77 to 90 d. Data were analyzed using GLIMMIX and UNIVARIATE in SAS 9.4. From 63 to 70 d, pigs fed diets with antibiotics had improved (P &lt; 0.05) average daily feed intake (ADFI), average daily weight gain (ADG), gain to feed ratio (G:F), and 70 d BW compared to those fed control or amino acid diets. From 70 to 77 d, including antibiotics in the diet increased (P &lt; 0.05) ADG and 77 d BW. From 77 to 90 d, pigs fed the amino acid diet had greater (P &lt; 0.05) ADG and ADFI than those fed an antibiotic diet. From 63 to 90 d, although pig performance was not affected (P &gt; 0.05), growth curve of pigs fed the antibiotic diets was different (P &lt; 0.05) from those fed the control and amino acids diets. At 70 d, serum tumor necrosis factor-α and diamine oxidase (DAO) were lower (P &lt; 0.05) in pigs fed the antibiotic diet than the control diet, and pigs fed the amino acid diet had intermediate results. Ferric reducing antioxidant power (FRAP) was lower (P &lt; 0.05) in pigs fed the amino acid diet than the antibiotic diet, and pigs fed the control diet had intermediate results. At 70 and 77 d, serum urea nitrogen was higher (P &lt; 0.05) in pigs fed the amino acid diet. At 77 d, DAO and serum immunoglobulin G was lower (P &lt; 0.05) in pigs fed the antibiotic diet. FRAP was lower (P &lt; 0.05) in pigs fed the amino acid and control diets. Serum malondialdehyde was higher (P &lt; 0.05) in pigs fed the amino acid diet than those fed the control diet, and pigs fed the antibiotic diet had intermediate results. At 90 d, antibiotics or amino acids did not affect (P &gt; 0.05) serum parameters. Amino acid blend supplementation at the selected doses in this study did not positively affect growing pigs. Although from 63 to 77 d, antibiotics improved performance, when considering the overall study period, growing pigs did not benefit from a diet containing antibiotics.

Effect of different levels of amino acids on carcass composition and yield in broilers

2011 ◽  
Vol 51 (12) ◽  
pp. 1123 ◽  
Author(s):  
Javad Nasr
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Group Treatment ◽  
National Research Council ◽  
Bodyweight Gain ◽  
Carcass Composition ◽  
Standard Group ◽  
Acid Diet ◽  
High Amino Acid ◽  
Different Levels

An experiment was conducted to evaluate the carcass yield and composition of 200 Arian male broilers fed diets (starter and grower) with different levels of amino acids requirements (high, medium, standard and low amino acids) in a completely randomised experimental design. All diets were isocaloric and isonitrogenous. Increased proportion of breast and thigh to bodyweight ratio were also obtained by increasing the level of amino acids from standard to high amino acids at 42 days of age (P < 0.05). In broilers receiving 120% of National Research Council amino acids, bodyweight at 42 days of age increased significantly by 202 g compared with standard amino acids diet. Feeding broilers with a high amino acid diet significantly (P < 0.05) increased carcass, breast, thigh and abdominal fat weights compared with standard group. Treatment with high amino acids had significantly higher levels of crude protein, lysine, and methionine percentage of breast and thigh meat in Arian broilers. The results of this study suggest that additional amino acids in starter and grower diets optimised bodyweight gain in Arian broiler, whereas reductions in amino acid levels reduced growth and liveweight.

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