scholarly journals Complex Biochemical Analysis of Fruiting Bodies from Newly Isolated Polish Flammulina velutipes Strains

2016 ◽  
Vol 65 (3) ◽  
pp. 295-305 ◽  
Author(s):  
Monika Osińska-Jaroszuk ◽  
Magdalena Jaszek ◽  
Justyna Sulej ◽  
Dawid Stefaniuk ◽  
Monika Urbaniak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Superoxide Dismutase ◽  
Nutritional Value ◽  
Biochemical Analysis ◽  
Rich Source ◽  
Cellobiose Dehydrogenase ◽  
Flammulina Velutipes ◽  
Fruiting Bodies ◽  
Potential Source ◽  
Phenolic Substances

The present study examined Polish strains of Flamulina velutipes as a potential source of nutraceuticals and found that their nutritional value is dependent on the fruiting bodies gathering time. To prove the above hypothesis protein, carbohydrate and phenolic substances concentration were determined. Moreover, catalase, superoxide dismutase, cellobiose dehydrogenase activities were assayed. In order to prove the healing properties of Enoki fruiting bodies the obtained extracts were tested for antioxidant and bacteriostatic abilities. We have proved that Polish F. velutipes fruiting bodies may be a rich source of antioxidants and that they are capable of inhibiting Staphylococcus aureus growth.

scholarly journals Attieké street vendors: a potential source for dissemination of virulent strains of Staphylococcus aureus in consumers

2019 ◽  
Vol 13 (3) ◽  
pp. 1420
Author(s):  
Sylvie Mireille Kouamé-Sina ◽  
Yakoura K. Ouattara ◽  
Fernique Konan ◽  
David Coulibaly N’golo ◽  
Kan Stephane Kouassi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Street Vendors ◽  
Potential Source ◽  
Virulent Strains

scholarly journals Effect of strains and extraction methods on β-glucan production, antioxidant properties, and FTIR Spectra from mushroom fruiting bodies of Schizophyllum commune Fr. in Thailand

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 410-415
Author(s):  
N. Mongkontanawat ◽  
D. Thumrongchote
Keyword(s):  
Antioxidant Activity ◽  
Chemical Structure ◽  
Nutritional Value ◽  
Antioxidant Properties ◽  
Schizophyllum Commune ◽  
Extraction Methods ◽  
Hot Water ◽  
Fruiting Bodies ◽  
Alkali Extraction ◽  
Medicinal Properties

Schizophyllum commune Fr., a native mushroom of Thailand, has a high nutritional value and is classified as a mushroom with medicinal properties that can neutralize the growth of many cancer cells. This research aimed to study the effect of S. commune strains and the extraction methods on the quantity and properties of β-glucan. The five S. commune Fr. strains used in this research consisted of Chanthaburi, 85-022, 85-023, 85-031, and 85- 043. There were two different β-glucan extraction methods employed: hot water (M1) and hot alkali extraction (M2), which were compared with the control (native-MR). The results indicated that the Chanthaburi strain has the highest β-glucan content 49.20±0.35% (w/w), and high potential antioxidant activity (79.14±0.77 DPPH% and 50.92±0.48 ABTS%) (p < 0.05). The extraction methods did not affect the yield of β-glucan, except the antioxidant properties and chemical structure of the extract substance. The extract substance from M2 has significantly the highest potential antioxidant activity (80.22±0.51). A mushroom juice drink in cans was developed using 1-day-old MR and adjusted pH of more than 7, which can increase the antioxidant properties of the product.

scholarly journals Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B

2019 ◽  
Vol 57 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Li-Hsin Huang ◽  
Hao-Yeh Lin ◽  
Ying-Tzu Lyu ◽  
Chiau-Ling Gung ◽  
Ching-Tsan Huang
Keyword(s):  
Hepatitis B ◽  
Oral Vaccine ◽  
Expression Level ◽  
Flammulina Velutipes ◽  
Total Soluble Protein ◽  
Control Group ◽  
Fruiting Bodies ◽  
Oral Vaccines ◽  
Virus Surface ◽  
Low Expression

Orally administered fungal vaccines show promise for the prevention of infectious diseases. Edible mushrooms are deemed appropriate hosts to produce oral vaccines due to their low production cost and low risk of gene contamination. However, their low expression level of antigens has limited the potential development of oral vaccines using mushrooms. The low expression level might result from impurity of the transgenic mycelia since dikaryotic mycelia are commonly used as transformation materials. In this study, stable transgenic hepatitis B virus surface antigen (HBsAg) in Flammulina velutipes transformants was obtained by Agrobacterium-mediated transformation, followed by fruiting and basidiospore mating. The formation of HBsAg was detected by western blot analysis. The expression levels of HBsAg in transgenic F. velutipes fruiting bodies were (129.3±15.1), (110.9±1.7) and (161.1±8.5) ng/g total soluble protein. However, the values may be underestimated due to incomplete protein extraction. Two of the four pigs in the experimental group produced positive anti-HBsAg-specific IgG after being fed the HBsAg transgenic F. velutipes fruiting bodies for 20 weeks, while no anti-HBsAg antibody was detected in the control group. One of the positive pigs had HBsAg titres of 5.36 and 14.9 mIU/mL in weeks 10 and 14, respectively, but expression faded thereafter. The other positive pig displayed HBsAg titres of 9.75, 17.86 and 39.87 mIU/mL in weeks 14, 18 and 20, respectively. The successful immunogenicity in pigs fed transgenic F. velutipes fruiting bodies demonstrated the potential of using the fungus as an oral vaccine.

scholarly journals Evaluation of Nutritional and Medicinal Values of Edible Wild and Cultivated Pleurotus ostreatus

2019 ◽  
Vol 7 (12) ◽  
pp. 2054
Author(s):  
Sanem Bulam ◽  
Nebahat Şule Üstün ◽  
Aysun Pekşen
Keyword(s):  
Pleurotus Ostreatus ◽  
Dairy Products ◽  
Nutritional Value ◽  
Large Scale ◽  
Essential Amino Acids ◽  
Fruiting Bodies ◽  
Mycelial Biomass ◽  
Biotechnological Processes ◽  
Medicinal Properties ◽  
Cultivation Techniques

Because of its high nutritional value and pharmaceutical effects, oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm.) is collected from nature and cultivated in large scale. This therapeutic mushroom is consumed as a functional food or food additive in soups, cereal and dairy products, and commercially used in nutraceuticals and dietary supplements. The mycochemicals including polysaccharides (crude fiber and β-glucans), essential amino acids, ergothioneine, peptides, (glyco)proteins, lectins, phenolic compounds, polyketides (lovastatin), (tri)terpenoids, and enzymes are naturally found in the fruiting bodies and mycelial biomass of P. ostreatus. The major bioactive compounds concentration of this mushroom may be increased by modification of the substrate composition and cultivation or postharvest conditions. The goal of this review is to evaluate the results of the studies about the biochemical composition and medicinal properties of edible wild and cultivated P. ostreatus. Furthermore, the advanced novel cultivation techniques, biotechnological processes, and postharvest treatments were given in order to increase its nutritional and nutraceutical values.

Re-distribution of Superoxide Dismutase is Protective Against Acute Lung Injury in Staphylococcus aureus Pneumonia

2020 ◽  
Vol 159 ◽  
pp. S47
Author(s):  
Christina Sul ◽  
Laura Hernandez-Lagunas ◽  
Nana Burns ◽  
Michelle Brajcich ◽  
Christine Vohwinkel ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Superoxide Dismutase ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Staphylococcus Aureus Pneumonia

scholarly journals Combining transcriptomics and metabolomics to reveal the underlying molecular mechanism of ergosterol biosynthesis during the fruiting process of Flammulina velutipes

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Ruihong Wang ◽  
Pengda Ma ◽  
Chen Li ◽  
Lingang Xiao ◽  
Zongsuo Liang ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Differentially Expressed ◽  
Flammulina Velutipes ◽  
Cdna Libraries ◽  
Ergosterol Biosynthesis ◽  
Biosynthesis Pathway ◽  
Fruiting Bodies ◽  
Regulatory Relationship ◽  
Illumina Hiseq ◽  
Hiv Drugs

Abstract Background Flammulina velutipes has been recognized as a useful basidiomycete with nutritional and medicinal values. Ergosterol, one of the main sterols of F. velutipes is an important precursor of novel anticancer and anti-HIV drugs. Therefore, many studies have focused on the biosynthesis of ergosterol and have attempted to upregulate its content in multiple organisms. Great progress has been made in understanding the regulation of ergosterol biosynthesis in Saccharomyces cerevisiae. However, this molecular mechanism in F. velutipes remains largely uncharacterized. Results In this study, nine cDNA libraries, prepared from mycelia, young fruiting bodies and mature fruiting bodies of F. velutipes (three replicate sets for each stage), were sequenced using the Illumina HiSeq™ 4000 platform, resulting in at least 6.63 Gb of clean reads from each library. We studied the changes in genes and metabolites in the ergosterol biosynthesis pathway of F. velutipes during the development of fruiting bodies. A total of 13 genes (6 upregulated and 7 downregulated) were differentially expressed during the development from mycelia to young fruiting bodies (T1), while only 1 gene (1 downregulated) was differentially expressed during the development from young fruiting bodies to mature fruiting bodies (T2). A total of 7 metabolites (3 increased and 4 reduced) were found to have changed in content during T1, and 4 metabolites (4 increased) were found to be different during T2. A conjoint analysis of the genome-wide connection network revealed that the metabolites that were more likely to be regulated were primarily in the post-squalene pathway. Conclusions This study provides useful information for understanding the regulation of ergosterol biosynthesis and the regulatory relationship between metabolites and genes in the ergosterol biosynthesis pathway during the development of fruiting bodies in F. velutipes.

scholarly journals Genomic and Biochemical Analysis of N Glycosylation in the Mushroom-Forming Basidiomycete Schizophyllum commune

2009 ◽  
Vol 75 (13) ◽  
pp. 4648-4652 ◽  
Author(s):  
Elsa Berends ◽  
Robin A. Ohm ◽  
Jan F. de Jong ◽  
Gerard Rouwendal ◽  
Han A. B. Wösten ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Schizophyllum Commune ◽  
Vegetative Mycelium ◽  
Fruiting Bodies ◽  
Genes Encoding

ABSTRACT N-linked glycans of Schizophyllum commune consist of Man5-9GlcNAc2 structures. Lack of further glycan maturation is explained by the absence of genes encoding such functions in this and other homobasidiomycetes. N-linked glycans in vegetative mycelium and fruiting bodies of S. commune are mainly Man7GlcNAc2 and Man5GlcNAc2, respectively, suggesting more efficient mannose trimming in the mushroom.

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