Optimizing Photoperiodism, Growth Media Dilution, and Frequency of Inoculum Addition in Microalgae (Chlorella vulgaris) Culture Using Bi-oslurry (ADDMW) as Growth Media

2020 ◽  
Vol 25 (1) ◽  
pp. 6-10
Author(s):  
T Taufikurahman ◽  
◽  
Teguh Suyadi ◽  
Keyword(s):  
Animal Feed ◽  
Biogas Production ◽  
Organic Content ◽  
Dairy Manure ◽  
Growth Media ◽  
Dilution Factor ◽  
Digestion Process ◽  
Microalgae Biomass ◽  
Anaerobically Digested Dairy Manure ◽  
Potential Use

In biogas production using dairy manure, an anaerobic digestion process also produced a by product bioslurry, also known as Anaerobically Digested Dairy Manure Wastewater (ADDMW), which is rich in ammonium and ortophosphate. ADDMW is potentially to be used as growth media to grow autotrophic organism, including microalgae. Furthermore, microalgae potential-ly could reduce organic content in ADDMW, thus play a role as phycoremediator to organic wastewater. In this study we used ADDMW medium for the growth of microalgae, determining its growth kinetics and level of reduction in ammonium and or-thophosphate content in ADDMW. In addition, we also analysed protein content in microalgae biomass for potential use as animal feed. The experiment was conducted using some variations in photoperiodism, i.e., 16:8; 12:12; and 8:16 and medium dilution factor of 2.5 and 5 times. Furthermore, frequency of inoculum addition was also examined i.e., a) 80 mL on day 0 (once); b) 26.67 mL on day 0 to 2 (first 3 days); and c) 8 mL on days 0 to 9 (every day). The results showed that 16:8 photo-period and 5 times medium dilution produced the highest biomass growth and productivity kinetics. Variation in frequency of inoculum addition showed no significant effect to a decrease in ammonium levels but indicated some effect to orthophosphate level. It can be concluded that microalgae can be grown in ADDMW media and can reduce its ammonium and ortophosphate content, as a phycoremediator, before the wastewater is dumped to the river.

Degradation of veterinary antibiotics during anaerobic digestion of dairy manure

2015 ◽  
Vol 10 (3) ◽  
pp. 532-537 ◽  
Author(s):  
Yumika Kitazono ◽  
Ikko Ihara ◽  
Kiyohiko Toyoda ◽  
Kazutaka Umetsu
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Dairy Manure ◽  
Antibiotic Residues ◽  
Veterinary Antibiotics ◽  
Livestock Manure ◽  
Anaerobic Digestion Process ◽  
Digestion Process ◽  
Antibiotic Degradation

This study evaluated antibiotic degradation and biogas production during anaerobic digestion of dairy manure contained two common veterinary antibiotics at 37 °C. After 18 days of digestion, the concentration of chlortetracycline (CTC) decreased more than 80% regardless of the initial CTC concentration. The concentration of cefazolin (CEZ) decreased from 10 to 0.08 mg/L in 6 days. Less than 50 mg/L CTC and 10 mg/L CEZ had negligible impact on biogas production during anaerobic digestion process. The result showed that the anaerobic digestion has a potential to degrade antibiotic residues in livestock manure.

Potential Use of Rum Distillery Slops as Animal Feed Supplement I. Mold Growth in Slops.

1969 ◽  
Vol 63 (3) ◽  
pp. 325-329
Author(s):  
Isabel M. González ◽  
Nivia F. Murphy
Keyword(s):  
Nutritional Value ◽  
Animal Feed ◽  
Growth Media ◽  
Mold Growth ◽  
Feed Supplement ◽  
Aspergillus Phoenicis ◽  
Potential Use

Consideration of the nutritional value of slops suggested its use as growth media for the production of fodder molds. Rum distillery slops supported better mold growth than the medium recommended for its growth. Best results were obtained with strain Aspergillus phoenicis isolated from contaminated slops in our laboratory.

scholarly journals Anaerobic Digestion and Codigestion of Chlorella Vulgaris Microalgae Biomass with Wastewater Sludge and Dairy Manure for Biogas Production

2017 ◽  
Vol 13 (3) ◽  
pp. 18-26 ◽  
Author(s):  
Saad H. Ammar ◽  
Sadiq Riyadh Khodhair
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Chlorella Vulgaris ◽  
Organic Waste ◽  
Biogas Production ◽  
Waste Product ◽  
Dairy Manure ◽  
Wastewater Sludge ◽  
Microalgae Biomass ◽  
Anaerobic Codigestion

Abstract   Anaerobic digestion process of organic materials is biochemical decomposition process done by two types of digestion bacteria in the absence of oxygen resulting in the biogas production, which is produced as a waste product of digestion. The first type of bacteria is known as acidogenic which converts organic waste to fatty acids. The second type of bacteria is called methane creators or methanogenic which transforms the fatty acids to biogas (CH4 and CO2). The considerable amounts of biodegradable constitutes such as carbohydrates, lipids and proteins present in the microalgae biomass make it a suitable substrate for the anaerobic digestion or even co-digested with other organic wastes. The present work investigated methane biogas production by anaerobic codigestion of microalgae, Chlorella vulgaris biomass with organic waste from several sources such as wastewater sludge and dairy manure waste in different proportions as an additional carbon supply to enhance anaerobic digestion and therefore biogas production. Six bottles, employed as batch biodigesters each of 1 liter capacity, were used for that purpose at moderate conditions (35±2 oC). The produced biogas volume was monitored daily along 35 days and the results showed that the daily and cumulative biogas production was increased 4.5 times and 3 times for the bottles with 66.67% microalgae compared with the bottles with wastewater sludge or dairy manure waste only, respectively.  Keywords: Anaerobic codigestion, biogas; dairy manure, microalgae Chlorella Vulgaris, wastewater sludge.

scholarly journals ANALISIS JUMLAH DAN MORFOLOGI Penicillium spp PADA MEDIA AMPAS TAHU

2019 ◽  
Vol 3 (2) ◽  
pp. 1
Author(s):  
Endah Prayekti ◽  
Thomas Sumarsono
Keyword(s):  
Animal Feed ◽  
Biogas Production ◽  
Nutrient Content ◽  
Morphological Characters ◽  
Growth Media ◽  
Commercial Media ◽  
Experimental Laboratory ◽  
The Media ◽  
The Difference ◽  
Penicillium Spp

Tofu waste is generally used for animal feed, potential for biogas production, and as a traditional food. Opportunities for the use of tofu waste are very wide because of the high nutrient content of tofu waste. Tofu waste nutrient content has the potential to be utilized as an alternative medium for mold growth. One type of mold that is often analyse in the laboratory is from the genusPenicillium. This study aims to determine the composition of an alternative growth media from tofu waste for Penicillium spp. This study is an experimental laboratory with the independent variable of tofu waste mass (0,1,2,3,4,5 gr) and sucrose mass (0,2,4,6 gr), while the dependent variable observed from this study includes the amount Penicillium spp colonies and their growth characteristics in tofu waste media. Both dependent variable observed then compared with Potato Dextrose Agar (PDA) as commercial media Tofu waste composition used in this study were (gr/100mL) : tofu waste (0, 1, 2, 3, 4, 5 gr), sucrose (0, 2, 4, 6 gr), aquadest (100mL), and agar powder (1,5gr). Statistical results using the Kruskal-Wallis Test showed the difference of the use of sucrose 2 and 6 gr in the media on thePenicillium spp colonies number. Colony morphology showed better performance at 5 gr of tofu waste mass. Morphological analysis shows the differences in morphological characters which are quite far from commercial media as a control using PDA. As conclusion, tofu waste media can be used to growPenicillium spp, but it is necessary to add some components both in the form of elements and compounds to tofu waste media to be able to display results that can approach commercial media.

Preliminary results of the optimization of biogas production at the biogas station of the national research institute of animal production in Grodziec Sląski – Kostkowice

2015 ◽  
Vol 2 (3) ◽  
pp. 26-31
Author(s):  
K. Węglarzy ◽  
Yu. Shliva ◽  
B. Matros ◽  
G. Sych
Keyword(s):  
Agricultural Production ◽  
Crude Protein ◽  
Biogas Production ◽  
Electric Energy ◽  
Corn Silage ◽  
Industrial Wastes ◽  
Organic Mass ◽  
National Research Institute ◽  
Digestion Process ◽  
By Products

Aim. To optimize the methane digestion process while using different recipes of substrate components of ag- ricultural origin. Methods. The chemical composition of separate components of the substrate of agricultural by-products, industrial wastes, fats of the agrorefi nery and corn silage was studied. Dry (organic) mass, crude protein (fat) fi ber, loose ash, nitrogen-free exhaust were estimated in the components and the productivity of biogas was determined along with the methane content. These data were used as a basis for daily recipes of the substrate and the analysis of biogas production at the biogas station in Kostkowice. Results. The application of by-products of agricultural production solves the problem of their storage on boards and in open containers, which reduces investment costs, related to the installation of units for their storage. Conclusions. The return on investment for obtaining electric energy out of agricultural biogas depends considerably on the kind of the substrate used and on technological and market conditions.

scholarly journals Investigation of Heavy Metal Effects on the Anaerobic Co-Digestion Process of Waste Activated Sludge and Septic Tank Sludge

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Quang-Minh Nguyen ◽  
Duy-Cam Bui ◽  
Thao Phuong ◽  
Van-Huong Doan ◽  
Thi-Nham Nguyen ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Activated Sludge ◽  
Removal Efficiency ◽  
Biogas Production ◽  
Cod Removal ◽  
Septic Tank ◽  
Waste Activated Sludge ◽  
Digestion Process ◽  
Cod Removal Efficiency

The effect of copper, zinc, chromium, and lead on the anaerobic co-digestion of waste activated sludge and septic tank sludge in Hanoi was studied in the fermentation tests by investigating the substrate degradation, biogas production, and process stability at the mesophilic fermentation. The tested heavy metals were in a range of concentrations between 19 and 80 ppm. After the anaerobic tests, the TS, VS, and COD removal efficiency was 4.12%, 9.01%, and 23.78% for the Cu(II) added sample. Similarly, the efficiencies of the Zn(II) sample were 1.71%, 13.87%, and 16.1% and Cr(VI) efficiencies were 15.28%, 6.6%, and 18.65%, while the TS, VS, and COD removal efficiency of the Pb(II) added sample was recorded at 16.1%, 17.66%, and 16.03% at the concentration of 80 ppm, respectively. Therefore, the biogas yield also decreased by 36.33%, 31.64%, 31.64%, and 30.60% for Cu(II), Zn(II), Cr(VI), and Pb(II) at the concentration of 80 ppm, compared to the raw sample, respectively. These results indicated that Cu(II) had more inhibiting effect on the anaerobic digestion of the sludge mixture than Zn(II), Cr(VI), and Pb(II). The relative toxicity of these heavy metals to the co-digestion process was as follows: Cu (the most toxic) > Zn > Cr > Pb (the least toxic). The anaerobic co-digestion process was inhibited at high heavy metal concentration, which resulted in decreased removal of organic substances and produced biogas.

Co-Digestion of Food Waste and Dairy Manure for Biogas Production

2007 ◽  
Vol 50 (5) ◽  
pp. 1815-1821 ◽  
Author(s):  
H. M. El-Mashad ◽  
R. Zhang
Keyword(s):  
Food Waste ◽  
Biogas Production ◽  
Dairy Manure

Study of thermal hydrolysis as a pretreatment to mesophilic anaerobic digestion of pig slurry

2001 ◽  
Vol 44 (4) ◽  
pp. 109-116 ◽  
Author(s):  
A. Bonmatí ◽  
X. Flotats ◽  
L. Mateu ◽  
E. Campos
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Limiting Factor ◽  
Pig Slurry ◽  
Thermal Hydrolysis ◽  
Organic Matter Degradation ◽  
Thermal Pretreatment ◽  
Digestion Process ◽  
Degradation Tests ◽  
Thermal Phenomena

Feasibility of anaerobic digestion of pig slurry is dependent, among other factors, on the biogas production rate, which is low compared with other organic wastes, and on the profitable uses of surplus thermal energy produced, a limiting factor in warm geographical areas. The objectives of this work are determining whether low temperature thermal pretreatment (<90°C) improves pig slurry anaerobic digestion, and determining whether organic matter degradation during the thermal pretreatment is due to thermal phenomena (80°C) or to enzymatic ones (60°C). The thermal degradation tests showed that hydrolysis occurring during the thermal pretreatment is due to thermal phenomena. The increase in soluble substances were significantly larger at 80°C than at 60°C (both during 3 h). Two types of slurry were used in the batch anaerobic digestion tests. The effect of thermal pretreatment differed with the type of slurry: it was positive with almost non-degraded slurries containing low NH4+-N concentration, and negative (inhibition of the anaerobic digestion process) when using degraded slurries with high NH4+-N content.

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