InstantLabs®Salmonella Species Food Safety Kit

2014 ◽  
Vol 97 (6) ◽  
pp. 1576-1584
Author(s):  
Neil Sharma ◽  
Lauren Bambusch ◽  
Thu Le ◽  
Melinda Hayman ◽  
Sergio J Montez
Keyword(s):  
Food Safety ◽  
Wheat Flour ◽  
Enrichment Culture ◽  
Hold Time ◽  
Reference Method ◽  
Test Method ◽  
Test Portion ◽  
Screen Result ◽  
Oat Flour ◽  
Better Than

Abstract The InstantLabs®Salmonella Species Food Safety Kit was validated against the International Organization for Standardization (ISO) reference method 6579:2002 for the detection of Salmonella species. The matrixes (unprocessed rolled oats, wheat flour, and oat flour) were inoculated with 1 CFU/test portion of Salmonella to generate fractional positives (5–15) in 20 inoculated samples. The matrixes were co-inoculated with Escherichia coli O157:H7 at 2–5 times the level of Salmonella to demonstrate the potential for using the same enrichment culture in the future to detect of multiple organisms. Samples were validated using 750 g test portion enriched in FASTGRO SE at 42 ± 1°C for 16–20 h. All samples were confirmed using the ISO reference method, regardless of initial screen result. The InstantLabs test method performed as well as or better than the reference method for the detection of Salmonella species in unprocessed rolled oats, wheat flour, and oat flour. Inclusivity and exclusivity testing revealed no false negatives and no false positives among the 100 Salmonella serovars and 30 non-Salmonella species examined. Finally, the method was shown to be robust when variations to enrichment time, DNA extract hold time, and DNA volume were varied (data not shown).

InstantLabs®E. coli O157 Food Safety Kit

2015 ◽  
Vol 98 (1) ◽  
pp. 71-81
Author(s):  
Neil Sharma ◽  
Lauren Bambusch ◽  
Apala Upadhyay ◽  
Thu Le ◽  
Chris Lopez ◽  
...  
Keyword(s):  
Food Safety ◽  
Enrichment Culture ◽  
Reference Method ◽  
Test Method ◽  
Romaine Lettuce ◽  
Test Portion ◽  
E Coli ◽  
Screen Result ◽  
Block Time ◽  
Better Than

Abstract The InstantLabs®E. coli O157 Food Safety Kit was validated against the International Organization for Standardizationreference method 16654 for the detection of Escherichia coli O157. The matrixes, raw ground beef, raw beef trim, Romaine lettuce, pasteurized apple juice, and raw ground chicken, were inoculated with appropriate CFU/test portion of E. coli O157 to generate fractional positives (5–15) in 20 inoculated samples. The matrixeswere co-inoculated with Salmonella at 2–5 times the level of E. coli O157 to demonstrate the potential for using the same enrichment culture for the detection of multiple organisms. Samples were enriched in prewarmed FASTGRO SE broth at 42 ± 1°C for 10–20 h. All samples were confirmed using the ISO reference method, regardless of initial screen result. The InstantLabs test method performed as well as or better than the reference method for the detection of E. coli O157 in all tested samples. Inclusivity and exclusivity testing revealed no false negatives and no false positives among the 50 E. coli O157 serovars and 30 non-E. coli O157 species examined. Finally, the method was shown to be robust when variations were applied to enrichment time, volume for DNA extraction, and heat block time.

InstantLabs®Salmonella Species Detection Method: Matrix Extension

2014 ◽  
Vol 97 (6) ◽  
pp. 1585-1591
Author(s):  
Neil Sharma ◽  
Lauren Bambusch ◽  
Thu Le ◽  
Amit Morey ◽  
Melinda Hayman ◽  
...  
Keyword(s):  
Ground Beef ◽  
Reference Method ◽  
Test Method ◽  
Chicken Breast ◽  
Escherichia Coli O157 ◽  
Test Portion ◽  
Screen Result ◽  
Low Levels ◽  
Matrix Extension ◽  
Better Than

Abstract The performance of InstantLabs®Salmonella Species Food Safety Kit to detect Salmonella in four food matrixes was validated against the International Organization for Standardization (ISO) reference method 6579:2002. The matrixes (raw ground beef, raw chicken breast, raw ground chicken, and lettuce) were inoculated with low levels of Salmonella (<1 CFU/test portion) to generate fractional positives (5–15) in 20 inoculated samples. These matrixes were co-inoculated with Escherichia coli O157:H7 at two to five times the level of Salmonella. Samples were validated using 375 g (meat) or 25 g (lettuce and poultry) test portions enriched in FASTGRO™ SE at 42 ± 1°C for 12 h and 10 h, respectively. All samples were confirmed using the ISO reference method, regardless of initial screen result. The InstantLabs test method was shown to perform as well as or better than the reference method for the detection of Salmonella species in ground beef, chicken breast, ground chicken, and lettuce. Inclusivity and exclusivity testing revealed no false negatives among the 100 Salmonella serovars and no false positives among the 30 non-Salmonella species examined, respectively.

Detection of Salmonella species in a Variety of Foods by the DuPont™ BAX® System Real-Time PCR Assay for Salmonella: First Action 2013.02

2014 ◽  
Vol 97 (3) ◽  
pp. 868-875 ◽  
Author(s):  
F Morgan Wallace ◽  
Bridget Andaloro ◽  
Dawn Fallon ◽  
Nisha Corrigan ◽  
Stephen Varkey ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Orange Juice ◽  
Collaborative Study ◽  
Reference Method ◽  
Probability Of Detection ◽  
Test Method ◽  
Pcr Assay ◽  
Test Portion ◽  
Significant Difference

Abstract A multilaboratory study was conducted to evaluate the ability of the DuPont™ BAX® System Real-Time PCR Assay for Salmonella to detect the target species in a variety of foods and environmental surfaces. Internal validation studies were performed by DuPont Nutrition & Health on 24 different sample types to demonstrate the reliability of the test method among a wide variety of sample types. Two of these matrixes—pork and turkey frankfurters and pasteurized, not-from-concentrate orange juice without pulp—were each evaluated in 14 independent laboratories as part of the collaborative study to demonstrate repeatability and reproducibility of the internal laboratory results independent of the end user. Frankfurter samples were evaluated against the U. S. Department of Agriculture, Food Safety and Inspection Service reference method as a paired study, while orange juice samples were evaluated against the U. S. Food and Drug Administration reference method as an unpaired study, using a proprietary media for the test method. Samples tested in this study were artificially inoculated with a Salmonella strain at levels expected to produce low (0.2–2.0 CFU/test portion) or high (5 CFU/test portion) spike levels on the day of analysis. For each matrix, the collaborative study failed to show a statistically significant difference between the candidate method and the reference method using the probability of detection statistical model.

InstantLabs®Listeria monocytogenes Food Safety Kit

2014 ◽  
Vol 97 (3) ◽  
pp. 852-861
Author(s):  
Neil Sharma ◽  
Lauren Bambusch ◽  
Thu Le ◽  
Amit Morey
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Reference Method ◽  
Cheddar Cheese ◽  
Ice Cream ◽  
Test Method ◽  
Listeria Species ◽  
Whole Milk ◽  
Raw Shrimp

Abstract The InstantLabs®Listeria monocytogenes Food Safety Kit was validated against the International Organization for Standardization (ISO) reference method 11290-1 for the detection of Listeria monocytogenes and other Listeria species. The matrixes (stainless steel, sealed concrete, ice cream, whole milk, cheddar cheese, raw shrimp, hot dogs, deli turkey, and lettuce) were inoculated with approximately 1 CFU/test portion of L. monocytogenes to generate fractional positives (5–15) in 20 inoculated samples. Enrichments were also fractionally inoculated with L. grayii for side-by-side testing of the Listeria Species Food Safety Kit. Stainless steel and sealed concrete samples were validated using 4 × 4″ and 1 × 1″ test areas, respectively, and enriched in Buffered Listeria Enrichment Broth (BLEB) at 35 ± 1°C for 22–28 h. All food samples were tested at 25 g and enriched in BLEB at 35 ± 1°C for 24–28 h. All samples were confirmed using the ISO reference method, regardless of initial screen result. The InstantLabs test method performed as well as or better than the reference method for the detection of L. monocytogenes on stainless steel and sealed concrete and in ice cream, whole milk, cheddar cheese, raw shrimp, hot dogs, deli turkey, and lettuce. Inclusivity and exclusivity testing revealed no false negatives and no false positives among the 50 L. monocytogenes serovars and 30 non-L. monocytogenes species examined. The method was shown to be robust when the enrichment times, volumes for DNA extraction, and heat block times were varied.

Method Modification of the Legipid®Legionella Fast Detection Test Kit

2014 ◽  
Vol 97 (5) ◽  
pp. 1403-1409 ◽  
Author(s):  
Guillermo Rodríguez Albalat ◽  
Begoña Bedrina Broch ◽  
Marisa Jiménez Bono
Keyword(s):  
Legionella Pneumophila ◽  
Reference Method ◽  
Culture Method ◽  
Test Method ◽  
Magnetic Microspheres ◽  
Test Portion ◽  
Relative Level ◽  
Fast Detection ◽  
Test Kit ◽  
Optical Reader

Abstract Legipid®Legionella Fast Detection is a test based on combined magnetic immunocapture and enzyme-immunoassay (CEIA) for the detection of Legionella in water. The test is based on the use of anti-Legionella antibodies immobilized on magnetic microspheres. Target microorganism is preconcentrated by filtration. Immunomagnetic analysis is applied on these preconcentrated water samples in a final test portion of 9 mL. The test kit was certified by the AOAC Research Institute as Performance Tested MethodSM (PTM) No. 111101 in a PTM validation which certifies the performance claims of the test method in comparison to the ISO reference method 11731-1998 and the revision 11731-2004 “Water Quality: Detection and Enumeration of Legionella pneumophila” in potable water, industrial water, and waste water. The modification of this test kit has been approved. The modification includes increasing the target analyte from L. pneumophila to Legionella species and adding an optical reader to the test method. In this study, 71 strains of Legionella spp. other than L. pneumophila were tested to determine its reactivity with the kit based on CEIA. All the strains of Legionella spp. tested by the CEIA test were confirmed positive by reference standard method ISO 11731. This test (PTM 111101) has been modified to include a final optical reading. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Two water matrixes were analyzed. Results show no statistically detectable difference between the test method and the reference culture method for the enumeration of Legionella spp. The relative level of detection was 93 CFU/volume examined (LOD50). For optical reading, the LOD was 40 CFU/volume examined and the LOQ was 60 CFU/volume examined. Results showed that the test Legipid Legionella Fast Detection is equivalent to the reference culture method for the enumeration of Legionella spp.

scholarly journals Applicability and performance of EUCAST’s rapid antimicrobial susceptibility testing (RAST) on primarily sterile body fluids in blood culture bottles in laboratory routine with total lab automation

2021 ◽  
Author(s):  
Jasmin Kaur Jasuja ◽  
Stefan Zimmermann ◽  
Irene Burckhardt
Keyword(s):  
Blood Culture ◽  
Susceptibility Testing ◽  
Reference Method ◽  
Body Fluids ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
And Performance ◽  
Sterile Body Fluids ◽  
Better Than

AbstractOptimisation of microbiological diagnostics in primarily sterile body fluids is required. Our objective was to apply EUCAST’s RAST on primarily sterile body fluids in blood culture bottles with total lab automation (TLA) and to compare results to our reference method Vitek2 in order to report susceptibility results earlier. Positive blood culture bottles (BACTEC™ Aerobic/Anaerobic/PEDS) inoculated with primarily sterile body fluids were semi-automatically subcultured onto Columbia 5% SB agar, chocolate agar, MacConkey agar, Schaedler/KV agar and Mueller-Hinton agar. On latter, cefoxitin, ampicillin, vancomycin, piperacillin/tazobactam, meropenem and ciprofloxacin were added. After 6 h, subcultures and RAST were imaged and MALDI-TOF MS was performed. Zone sizes were digitally measured and interpreted following RAST breakpoints for blood cultures. MIC values were determined using Vitek2 panels. During a 1-year period, 197 Staphylococcus aureus, 91 Enterococcus spp., 38 Escherichia coli, 11 Klebsiella pneumoniae and 8 Pseudomonas aeruginosa were found. Categorical agreement between RAST and MIC was 96.5%. Comparison showed no very major errors, 2/7 (28.6%) and 1/7 (14.3%) of major errors for P. aeruginosa and meropenem and ciprofloxacin, 1/9 (11.1%) for K. pneumoniae and ciprofloxacin, 4/69 (7.0%) and 3/43 (5.8%) for Enterococcus spp. and vancomycin and ampicillin, respectively. Minor errors for P. aeruginosa and meropenem (1/8; 12.8%) and for E. coli and ciprofloxacin (2/29; 6.5%) were found. 30/550 RAST measurements were within area of technical uncertainty. RAST is applicable and performs well for primarily sterile body fluids in blood culture bottles, partially better than blood-based RAST. Official EUCAST evaluation is needed.

scholarly journals Performance of elderly on the three words-three shapes test: A Brazilian study

2007 ◽  
Vol 1 (4) ◽  
pp. 396-401
Author(s):  
Cristiane Garcia da Costa Armentano ◽  
Julieta Quayle
Keyword(s):  
Educational Level ◽  
The Elderly ◽  
Test Method ◽  
Delayed Recall ◽  
Assessment Of Learning ◽  
Significant Difference ◽  
Males And Females ◽  
Stored Information ◽  
Better Than ◽  
Years Of Schooling

Abstract The three words-three shapes test is a brief bedside technique for assessment of learning and memory using verbal and non-verbal material. To the best of our knowledge, performance of Brazilian elderly on this test has not yet been reported. Objective: To evaluate the performance of normal Brazilian elderly on the three words-three shapes test. Method: A total of 50 adult patients, 25 males and 25 females, with age ranging from 55 to 81 years (66.0±7.10 years), 1 to 8 years of schooling, different economic conditions and living in the São José do Rio Preto municipality, State of São Paulo, were evaluated. Results: There was no statistically significant difference between performance of males and females. Performance on incidental recall was significantly lower than in delayed recall. The performance in the learning phase improved following at least two further presentations of the stimuli. Approximately 50% of the participants did not remember the six stimuli and had to proceed to the recognition stage. The performance in the recognition stage was significantly better than during spontaneous recall. Patients with low educational level (less years of schooling) had poorer performance on the recall of shapes and on the total score of the test. Conclusions: The three words-three shapes test is rapid, efficient and straightforward to apply in the elderly, but low educational level was associated with poorer performance on this test. Normal elderly individuals had greater difficulty in the encoding process and in searching for stored information.

scholarly journals PEMANFAATAN BIJI KETAPANG (Terminalia catappa) SEBAGAI SUMBER PROTEIN DAN SERAT PADA PRODUK MAKANAN STIK

2019 ◽  
Vol 1 (1) ◽  
pp. 27-33
Author(s):  
Eman Darmawan
Keyword(s):  
Water Content ◽  
Wheat Flour ◽  
Test Method ◽  
Snack Food ◽  
Organoleptic Properties ◽  
Seed Flour ◽  
A Value ◽  
Randomized Design ◽  
Yellow Color ◽  
Completely Randomized Design

The purpose of this study was to determine the effect of substitution ketapan seed to chemical and organoleptic properties of  the resulted snack food, knowing the exact level of substitution, so obtain the snack food liked the panelists. The design used in this study was completely randomized design (CRD) with single factor that influences the concentration of seed flour substitution ketapan seed consisting of 5 treatments. Each treatment be repeated 3 times. The data obtained was analyzed by Analysis of Variance (ANOVA), if there was a difference between the treatment of advanced test conducted by Duncan`s Multiple Ranges Test Method (DMRT) at the level of 5%. Substitution of wheat flour with ketapan seed flour  affects the snack food produced, which can reduce the water content of the snack food and increase levels of protein and fiber snack food. Ketapan seed flour substitution preferred by the panelists was substitution ketapan seed flour up to 30% with a value of 3.52 and the criteria snack food produced had a water content of 3.67%, 15.10% db protein content, fiber content of 3.64 % db, brownish yellow color (2.95), a rather tasted wheat flour (3.35), and crispy (3.60).

scholarly journals PENDIDIKAN KARAKTER MELALUI PEMBELAJARAN MATEMATIKA

2014 ◽  
Vol 10 (1) ◽  
Author(s):  
Salafudin *
Keyword(s):  
Statistical Analysis ◽  
Character Education ◽  
Sampling Technique ◽  
Problem Formulation ◽  
Test Method ◽  
Cluster Sampling ◽  
Test Results ◽  
Learning Mathematics ◽  
Character Building ◽  
Better Than

This study aimed to determine: (1) how was the design of character building by learning mathematics, and (2) whether the character building by learning mathematics could produce better learning outcome of mathematics than conventional mathematics learning.To answer these two questions, I used Research and Development ( R & D ) which consists of a preliminary study to obtain preliminary data on student learning outcomes before being given treatments of character building by learning mathematics. The next phase is the design of the character building by learning mathematics. Further testing and revision in class and repeated until it found the best learning model. From the first problem formulation, qualitative data analysis, such as the design of character building in learning mathematics was produced. The results of the qualitative analysis shows, character building by learning mathematics was quite effectively applied to implant positive character in students. To answer the second question, statistical analysis was used. The population in this study was all students of class VII of MTsN in Buaran of Pekalongan which totaled 220. By random cluster sampling technique two classes (a class VII A and VII B) was chosen. Class VII A was as an experiment class, and class VII B was as a control class. Data were taken by the test method, experimental classroom observation, and documentation. The data was then processed with an average difference test. Results of statistical analysis obtained t = 3.33 > t table = 1.67. This meant that learning achievement of the students in the experimental class, which was implemented character education in mathematics was better than the students who were taught by conventional methods. Based on these results, it could be conclude that the character building in mathematics was better than the conventional method of learning mathematics.

scholarly journals Determination of Fat in Raw and Processed Milks by the Gerber Method: Collaborative Study

2001 ◽  
Vol 84 (5) ◽  
pp. 1499-1508 ◽  
Author(s):  
Dick H Kleyn ◽  
Joanna M Lynch ◽  
David M Barbano ◽  
M Jeffrey Bloom ◽  
Martin W Mitchell ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Reference Method ◽  
Fat Content ◽  
Interlaboratory Study ◽  
Test Portion ◽  
Ether Extraction ◽  
Whole Milk ◽  
Relative Standard ◽  
Extraction Test ◽  
Fat Level

Abstract The Gerber method is used worldwide as a simple and rapid method for determining fat in raw and processed milks. However, the volume of the test portion used in the method has not been internationally agreed upon. A collaborative study was conducted to evaluate performance of the Gerber method using either a weighed test portion (11.13 g) or by a 10.77 mL test portion delivered by pipet. For each method, laboratories received 10 test samples: 5 raw and 5 pasteurized homogenized milks, 2 of which were blind duplicate pairs. Eleven and 10 laboratories participated in the evaluation of aliquot addition by weight and pipet, respectively. Mojonnier ether extraction (Method 989.05) was used as the reference method. Interlaboratory study statistics were similar between methods of test portion addition and between raw and processed materials; therefore, summary interlaboratory study statistics were pooled. The fat content of milk samples ranged from 0.96 to 5.48%. Absolute reproducibility and repeatability were not affected by fat level, and pooled statistical performance (invalid and outlier data removed) was (g fat/100 g milk) sr = 0.026, sR = 0.047, r = 0.074, and R = 0.132. Relative standard deviations increased with decreasing fat content, and were summarized by fat level: 1–2% fat milk, mean = 1.437, RSDr = 1.809%, RSDR = 3.271%; 2–6% fat milk, mean = 4.156, RSDr = 0.626%, RSDR = 1.131%. Compared with ether extraction, test results by the Gerber method were slightly lower (0.02% fat) using a weighed test portion and significantly lower (0.06% fat) using a 10.77 mL volume addition by pipet. A trend toward underestimating fat content at lower fat concentrations (1–2% fat) was observed with the weighed test portion but not when a pipet was used. The Associate Referee recommends that the Gerber method using a weighed test portion be adopted as First Action with applicability limited to whole milk.

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