Determination of Aflatoxins in Edible Oils from China and Ethiopia Using Immunoaffinity Column and HPLC-MS/MS

2019 ◽  
Vol 102 (1) ◽  
pp. 149-155 ◽  
Author(s):  
Lingyun Chen ◽  
Alemu Eshetie Molla ◽  
Kassa Metsehet Getu ◽  
Ande Ma ◽  
Chengsong Wan
Keyword(s):  
Edible Oils ◽  
Total Concentration ◽  
Edible Oil ◽  
Sub Saharan Africa ◽  
National Standard ◽  
Human Consumption ◽  
Immunoaffinity Column ◽  
Allowable Limit ◽  
Policy Makers

Abstract Background: Aflatoxin (AF) ingestion through contaminated foodstuffs causes at least 250 000 deaths every year from hepatocellular carcinoma in China and sub-Saharan Africa. Objective: The main objective of the study was to determine the aflatoxin levels of oils in South Gondar, Ethiopia, and oils purchased from retail markets in Guangzhou, China. Methods: We used a rapid, sensitive, and selective HPLC-tandem mass spectrometry (MS/MS) method for the determination of aflatoxins in edible oils from China and Ethiopia using immunoaffinity column cleaning. Results: The level of contamination for Ethiopian oils ranged between 0.07 and 145.59 μg/kg for total aflatoxins. Of the 27 edible oil samples from Guangzhou, China, the total concentration of aflatoxins (AFB1 + AFB2 + AFG1 + AFG2) ranged between 0.03 and 2.23 μg/kg. Conclusions: The study concluded that the peanut oils from Ethiopia were contaminated with aflatoxins higher than the allowable limit set by many countries while the oils from China were safe for human consumption. Highlights: We first describe an HPLC-MS/MS method for the determination of aflatoxins in 48 edible oil samples from China and Ethiopia using immunoaffinity column cleaning. This is the first preliminary study done on Ethiopian edible oils, giving policy-makers and future researchers baseline data. It is also used to assess the aflatoxin levels of the Chinese edible oils from Guangzhou. Therefore, conducting a comparative study points out the severity of the problem and helps to formulate a new national standard for policy-makers, making this study imperative.

scholarly journals Trace elements determination by ICP-QMS in octopus edible samples from Mexico City markets

2017 ◽  
Vol 57 (4) ◽  
Author(s):  
L. V.R. Saldívar-Osorio ◽  
M. T.J. Rodríguez-Salazar ◽  
M. L. Soubran-Zamora Soubran-Zamora ◽  
C. E. Márquez-Herrera ◽  
M. E. Castilla-Madrigal ◽  
...  
Keyword(s):  
Trace Elements ◽  
Mexico City ◽  
Inductively Coupled Plasma ◽  
Total Concentration ◽  
Mean Value ◽  
Human Consumption ◽  
Inductively Coupled ◽  
Distrito Federal ◽  
Mean Concentration

The purpose of this study was to evaluate the total concentration levels of trace elements (Cr, Cu, Mn, Pb, Rb and Sr) in octopus’ mantle and tentacles samples from Mexico City markets, by using the Mexican regulation applied to food for human consumption. The determination of both essential (Cr, Cu, Mn) and non-essential (Pb, Rb and Sr) trace elements was made by inductively coupled plasma-quadrupole mass spectrometry (ICP-QMS). The samples were collected from local markets and supermarkets from each of 16 delegations of Mexico City, Distrito Federal. The overall ranges of concentrations found were: 2.4-4.8, 5.1-95.3, 1-6, 0.1-1.6, 0.4-4.5 and 11-58 mg/kg for Cr, Cu, Mn, Pb, Rb and Sr, respectively. The maximum and mean concentration values obtained for Cu and Cr in mantle and tentacle samples from both kind of markets, are above of the NOM-051-SCFI/SSA1-2010 (closest Mexican standard), according to USDA National NDB for Standard Reference consumer exposure (100g/day) of octopus. It was observed that the maximum Pb concentration value found in mantle and tentacle samples from supermarkets origin exceeds the NOM-129-SSA1-1995 and NOM-242-SSA1-2009 reference values, while the maximum mean value is below the regulation. Similar Mexican regulation data for Mn, Rb and Sr were not found.

scholarly journals Determination of Aflatoxins B1, B2, G1, and G2 in Olive Oil, Peanut Oil, and Sesame Oil

2010 ◽  
Vol 93 (3) ◽  
pp. 936-942 ◽  
Author(s):  
Lei Bao ◽  
Mary W Trucksess ◽  
Kevin D White
Keyword(s):  
Olive Oil ◽  
Edible Oils ◽  
Lower Layer ◽  
Multiple Reaction Monitoring ◽  
Animal Health ◽  
Sesame Oil ◽  
Peanut Oil ◽  
Immunoaffinity Column ◽  
Accuracy And Repeatability

Abstract Edible oils are consumed directly, and used as ingredients in food, soaps, and skin products. However, oils such as olive oil, peanut oil, and sesame oil could be contaminated with aflatoxins, which are detrimental to human and animal health. A method using immunoaffinity column cleanup with RPLC separation and fluorescence detection (FLD) for determination of aflatoxins (AF) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil was developed and validated. Test samples were extracted with methanolwater (55 + 45, v/v). After shaking and centrifuging, the lower layer was filtered, diluted with water, and filtered through glass microfiber filter paper. The filtrate was then passed through an immunoaffinity column, and the toxins were eluted with methanol. The toxins were then subjected to RPLC/FLD analysis after postcolumn UV photochemical derivatization. The accuracy and repeatability characteristics of the method were determined. Recoveries of AFB1 spiked at levels from 1.0 to 10.0 g/kg in olive oil, peanut oil, and sesame oil ranged from 82.9 to 98.6. RSDs ranged from 0.6 to 8.9. HorRat values were <0.2 for all of the matrixes tested. Recoveries of AF spiked at levels from 2.0 to 20.0 g/kg ranged from 87.7 to 102.2. RSDs ranged from 1.3 to 12.6. HorRat values were <0.4 for all of the matrixes tested. LC/MS/MS with multiple-reaction monitoring was used to confirm the identities of aflatoxins in a naturally contaminated peanut oil.

Determination of Abamectin Residues in Edible Oils by High-Performance Liquid Chromatography with Fluorescence Detection

2014 ◽  
Vol 97 (3) ◽  
pp. 928-932 ◽  
Author(s):  
Xue-Xue Miao ◽  
Yuan-Yuan Yang ◽  
Xiao-Yun Yang ◽  
Qi-Liang Huang ◽  
Hai Hong
Keyword(s):  
High Performance Liquid Chromatography ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
High Performance ◽  
Detection Method ◽  
Edible Oils ◽  
Edible Oil ◽  
Time Saving ◽  
Satisfactory Precision

Abstract A rapid and simple HPLC-fluorescence detection method has been developed for the determination of abamectin residues in edible oil. Residues are extracted with acetonitrile and by vortexing and then directly derivatized with no need for a time-consuming cleanup step. Trifluoroacetic anhydride and N-methylimidazole were used as derivatizing agents of abamectin. Abamectin was detected and quantitated with fluorescence detection (excitation: 365 nm; emission: 475 nm), and methanol was used as the mobile phase. The LOD was 0.001 mg/kg and the LOQ was 0.003 mg/kg. The recoveriesranged from 86 to 100.4% with satisfactory precision (RSD < 10.1%). This method proved to be sensitive, environmentally friendly, time-saving, and efficient.

scholarly journals Comparison of a Biosensor and a Commercial UV-Visible Method for Measuring Hexavalent Chromium in Liquid Medium

2021 ◽  
Author(s):  
Melany Avellaneda ◽  
Santiago Xavier Mafla ◽  
Moraima Mera
Keyword(s):  
Hexavalent Chromium ◽  
Gene Transformation ◽  
Coefficient Of Determination ◽  
Human Consumption ◽  
Allowable Limit ◽  
E Coli ◽  
Margin Of Error ◽  
Uv Visible ◽  
Bacteria Resistance

Abstract The objective of the research was to contrast two methods for the quantification of hexavalent chromium. The first method is the biosensor that from the gene transformation of the cells of Escherichia coli, was incorporated by electroporation the plasmid pTOP Blunt V2, synthesized with luxA genes that provides luminescence through the catalytic activity of the luciferase top and chr genes that give the bacteria resistance to chromium. The second method is the application of the UV-visible colorimetric technique. Chromium was analysed at different concentrations, from 0.05 mg l−1 (maximum allowable limit for human consumption); 0.1 mg l−1; 0.2 mg l−1; 0.4 mg l−1; 0.8 mg l−1 and 1 mg l−1 with 5 replicates, subsequent to this, the two methods of chromium analysis were applied in river samples, thus obtaining that the biosensor in concentrations of 2x106 CFU of E. coli, has a margin of error of 1.4%, a result derived from the coefficient of determination of the absorbance of chromium, unlike the UV-visible method with the colorimetric equipment, which presented a reading error of 3.9%.

Detection of Phthalate Esters from Plastic Packaging Materials into Edible Oil by Gas Chromatography-Mass

2013 ◽  
Vol 395-396 ◽  
pp. 355-358 ◽  
Author(s):  
Lin Li ◽  
Qiu Ju Sun ◽  
Shi Gang Xin ◽  
Long Yu ◽  
Zhong Li Jiang
Keyword(s):  
Gas Chromatography ◽  
Edible Oils ◽  
Phthalate Esters ◽  
Correlation Coefficients ◽  
High Sensitivity ◽  
Edible Oil ◽  
Relative Standard ◽  
Two Samples ◽  
Storage Temperatures

A method for the simultaneous determination of 15 kinds of phthalate esters in edible oil by gas chromatography-mass spectrometer (GC-MS) was firstly established. The calibration curves showed good linearity in the range of 0.001~1mg /L and the correlation coefficients (r) were more than 0.999. The limits of quantification were in the range of 0.001~ 2.000 μg/L (S/N = 10). The recovery of three level ranged from 70.50% to 112.00%, at spiked levels of 0.1mg/L, 1.0mg/L and 10mg/L with the relative standard deviations (n = 6) of 0.59% ~ 7.54%. On the basis of the above method, the three edible oils under different storage temperatures were detected and the results showed that DIBP, DBP and DEHP were detected in two samples. This method possesses high sensitivity and low detection limit and can be used for quantitative analysis PAEs in edible oils.

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