scholarly journals Thriving at Low pH: Adaptation Mechanisms of Acidophiles

2021 ◽  
Author(s):  
Xianke Chen
Keyword(s):  
Electrical Potential ◽  
Acid Tolerance ◽  
Acid Resistance ◽  
Intelligent Manufacturing ◽  
Membrane Composition ◽  
Ph Homeostasis ◽  
Protein Repair ◽  
A Cell ◽  
Transmembrane Electrical Potential

Acid resistance of acidophiles is the result of long-term co-evolution and natural selection of acidophiles and their natural habitats, and formed a relatively optimal acid-resistance network in acidophiles. The acid tolerance network of acidophiles could be classified into active and passive mechanisms. The active mechanisms mainly include the proton efflux and consumption systems, generation of reversed transmembrane electrical potential, and adjustment of cell membrane composition; the passive mechanisms mainly include the DNA and protein repair systems, chemotaxis and cell motility, and quorum sensing system. The maintenance of pH homeostasis is a cell-wide physiological process that adopt differently adjustment strategies, deployment modules, and integration network depending on the cell’s own potential and its habitat environments. However, acidophiles exhibit obvious strategies and modules similarities on acid resistance because of the long-term evolution. Therefore, a comprehensive understanding of acid tolerance network of acidophiles would be helpful for the intelligent manufacturing and industrial application of acidophiles.

scholarly journals Interplay between a cytosolic and a cell surface carbonic anhydrase in pH homeostasis and acid tolerance ofLeishmania

2017 ◽  
Vol 130 (4) ◽  
pp. 754-766 ◽  
Author(s):  
Dhiman Sankar Pal ◽  
Mazharul Abbasi ◽  
Dipon Kumar Mondal ◽  
Binitha Anu Varghese ◽  
Ritama Paul ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Cell Surface ◽  
Acid Tolerance ◽  
Ph Homeostasis ◽  
A Cell

scholarly journals Bioenergetic properties and viability of alkalophilic Bacillus firmus RAB as a function of pH and Na+ contents of the incubation medium

1982 ◽  
Vol 152 (3) ◽  
pp. 1096-1104
Author(s):  
M Kitada ◽  
A A Guffanti ◽  
T A Krulwich
Keyword(s):  
Electrical Potential ◽  
Alkaline Ph ◽  
Bacillus Firmus ◽  
Ph Homeostasis ◽  
Proton Extrusion ◽  
Whole Cells ◽  
Cell Functions ◽  
Transmembrane Electrical Potential ◽  
Alkalophilic Bacillus ◽  
Dependent Mechanism

The bioenergetic properties and viability of obligately alkalophilic Bacillus firmus RAB have been examined upon incubation in alkaline and neutral buffers in the presence or absence of added Na+. At pH 10.5, cells incubated in the absence of Na+ exhibited an immediate rise in cytoplasmic pH from less than 9.5 to 10.5, and they lost viability very rapidly. Viability experiments in the presence or absence of an energy source further suggested that the Na+-dependent mechanism for pH homeostasis is an energy-requiring function. The Na+/H+ antiporter, which catalyzes the vital proton accumulation at alkaline pH, was only slightly operational at pH 7.0; both whole cells and vesicles exhibited net proton extrusion even in the presence of Na+. Moreover, cells incubated in buffer at pH 7.0 were actually more viable in the presence of Na+ than in its absence. Thus, the inability of B. firmus RAB to grow at neutral pH is not due to excessive acidification of the cytoplasm. Rather, the transmembrane electrical potential, delta psi, generated at pH 7.0 was found to be much lower than at alkaline pH. The very low delta psi compromised several cell functions, e.g., Na+/solute symport and motility, which in this and other alkalophiles specifically depend upon delta psi and Na+.

The mitochondrial consequences of uncoupling intact cells depend on the nature of the exogenous substrate

2001 ◽  
Vol 355 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Brigitte SIBILLE ◽  
Céline FILIPPI ◽  
Marie-Astrid PIQUET ◽  
Pascale LECLERCQ ◽  
Eric FONTAINE ◽  
...  
Keyword(s):  
Oxidation Rate ◽  
Marked Decrease ◽  
Electrical Potential ◽  
Atp Synthesis ◽  
Intact Cells ◽  
Isolated Mitochondria ◽  
Exogenous Substrate ◽  
High Oxidation ◽  
Transmembrane Electrical Potential ◽  
Sustained Increase

In isolated mitochondria the consequences of oxidative phosphorylation uncoupling are well defined, whereas in intact cells various effects have been described. Uncoupling liver cells with 2,4-dinitrophenol (DNP) in the presence of dihydroxyacetone (DHA) and ethanol results in a marked decrease in mitochondrial transmembrane electrical potential (∆ψ), ATP/ADP ratios and gluconeogenesis (as an ATP-utilizing process), whereas the increased oxidation rate is limited and transient. Conversely, when DHA is associated with octanoate or proline, DNP addition results in a very large and sustained increase in oxidation rate, whereas the decreases in ∆ψ, ATP/ADP ratios and gluconeogenesis are significantly less when compared with DHA and ethanol. Hence significant energy wastage (high oxidation rate) by uncoupling is achieved only with substrates that are directly oxidized in the mitochondrial matrix. Conversely in the presence of substrates that are first oxidized in the cytosol, uncoupling results in a profound decrease in mitochondrial ∆ψ and ATP synthesis, whereas energy wastage is very limited.

Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney

2008 ◽  
Vol 34 (4) ◽  
pp. 367-372 ◽  
Author(s):  
P. Ciba ◽  
S. Schicktanz ◽  
E. Anders ◽  
E. Siegl ◽  
A. Stielow ◽  
...  
Keyword(s):  
Cell Population ◽  
Head Kidney ◽  
Siberian Sturgeon ◽  
Acipenser Baerii ◽  
Long Term Culture ◽  
A Cell

scholarly journals Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance

2012 ◽  
Vol 12 (1) ◽  
pp. 8 ◽  
Author(s):  
Nathan P King ◽  
Türkan Sakinç ◽  
Nouri L Ben Zakour ◽  
Makrina Totsika ◽  
Begoña Heras ◽  
...  
Keyword(s):  
Cell Wall ◽  
Linoleic Acid ◽  
Acid Resistance ◽  
Staphylococcus Saprophyticus ◽  
A Cell

scholarly journals Thickness and corrosion resistance optimization of Micro-ARC oxidation coating on Mg-Al-Li-Zn alloy using Taguchi approach

2020 ◽  
pp. 62-66
Author(s):  
Do Le Hung Toan, Shuo-Jen Lee Do
Keyword(s):  
Corrosion Resistance ◽  
Processing Time ◽  
Electrical Potential ◽  
Surface Protection ◽  
Alkaline Electrolytes ◽  
Micro Arc Oxidation ◽  
Corrosive Environments ◽  
Zn Alloy ◽  
Main Factor

Micro arc oxidation method has been developed in the field of surface protection of magnesium alloys and considered as a simple, highly effective, commercial and environmentally friendly method in industry. MAO coatings are fabricated on novel Mg-Al-Li-Zn alloy to improve the anti-corrosion performance of surface by using friendly alkaline electrolytes under a high electrical potential. The Taguchi method and optimal analysis are used to identify the effects of the three factors including current density, processing time and electrical frequency on coating’s characteristics. The results have shown that the main factor that affects coating thickness and corrosion resistance of coating is the processing time. The results obtained by optimal conditions are consistent with prediction values of Taguchi analysis. The thickness of the coating can help to improve the long-term corrosion protection of a MAO coating in corrosive environments.

scholarly journals A Stochastic Model for Virus Growth in a Cell Population

2014 ◽  
Vol 51 (3) ◽  
pp. 599-612 ◽  
Author(s):  
J. E. Björnberg ◽  
T. Britton ◽  
E. I. Broman ◽  
E. Natan
Keyword(s):  
Stochastic Model ◽  
Host Cell ◽  
Cell Population ◽  
Branching Process ◽  
Virus Population ◽  
Virus Growth ◽  
Optimal Balance ◽  
Large Numbers ◽  
A Cell

In this work we introduce a stochastic model for the spread of a virus in a cell population where the virus has two ways of spreading: either by allowing its host cell to live and duplicate, or by multiplying in large numbers within the host cell, causing the host cell to burst and thereby let the virus enter new uninfected cells. The model is a kind of interacting Markov branching process. We focus in particular on the probability that the virus population survives and how this depends on a certain parameter λ which quantifies the ‘aggressiveness’ of the virus. Our main goal is to determine the optimal balance between aggressive growth and long-term success. Our analysis shows that the optimal strategy of the virus (in terms of survival) is obtained when the virus has no effect on the host cell's life cycle, corresponding to λ = 0. This is in agreement with experimental data about real viruses.

scholarly journals Carbonic Anhydrases: New Perspectives on Protein Functional Role and Inhibition in Helicobacter pylori

2021 ◽  
Vol 12 ◽  
Author(s):  
Cristina Campestre ◽  
Viviana De Luca ◽  
Simone Carradori ◽  
Rossella Grande ◽  
Vincenzo Carginale ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Pathogenic Bacteria ◽  
Acid Tolerance ◽  
Membrane Vesicles ◽  
Research Field ◽  
Outer Membrane Vesicles ◽  
Bacterial Toxin ◽  
Carbonic Anhydrases ◽  
Ph Homeostasis ◽  
H Pylori

Our understanding of the function of bacterial carbonic anhydrases (CAs, EC 4.2.1.1) has increased significantly in the last years. CAs are metalloenzymes able to modulate CO2, HCO3– and H+ concentration through their crucial role in catalysis of reversible CO2 hydration (CO2 + H2O ⇄ HCO3– + H+). In all living organisms, CA activity is linked to physiological processes, such as those related to the transport and supply of CO2 or HCO3–, pH homeostasis, secretion of electrolytes, biosynthetic processes and photosynthesis. These important processes cannot be ensured by the very low rate of the non-catalyzed reaction of CO2 hydration. It has been recently shown that CAs are important biomolecules for many bacteria involved in human infections, such as Vibrio cholerae, Brucella suis, Salmonella enterica, Pseudomonas aeruginosa, and Helicobacter pylori. In these species, CA activity promotes microorganism growth and adaptation in the host, or modulates bacterial toxin production and virulence. In this review, recent literature in this research field and some of the above-mentioned issues are discussed, namely: (i) the implication of CAs from bacterial pathogens in determining the microorganism growth and virulence; (ii) the druggability of these enzymes using classical CA inhibitors (CAIs) of the sulfonamide-type as examples; (iii) the role played by Helicobacter pylori CAs in the acid tolerance/adaptation of the microbe within the human abdomen; (iv) the role of CAs played in the outer membrane vesicles spawned by H. pylori in its planktonic and biofilm phenotypes; (v) the possibility of using H. pylori CAIs in combination with probiotic strains as a novel anti-ulcer treatment approach. The latter approach may represent an innovative and successful strategy to fight gastric infections in the era of increasing resistance of pathogenic bacteria to classical antibiotics.

scholarly journals Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor

2019 ◽  
Author(s):  
Walter Thavarajah ◽  
Adam D. Silverman ◽  
Matthew S. Verosloff ◽  
Nancy Kelley-Loughnane ◽  
Michael C. Jewett ◽  
...  
Keyword(s):  
Water Source ◽  
Free System ◽  
Molecular Systems ◽  
Long Term Storage ◽  
Point Of Use ◽  
Regulatory Standard ◽  
A Cell ◽  
Global Concern ◽  
Dna Template

AbstractAdvances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 parts per million, the EPA’s most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.

scholarly journals Short term but highly efficient Cas9 expression mediated by excisional system using adenovirus vector and Cre

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sayaka Nagamoto ◽  
Miyuki Agawa ◽  
Emi Tsuchitani ◽  
Kazunori Akimoto ◽  
Saki Kondo Matsushima ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Genome Editing ◽  
Adenovirus Vector ◽  
Virus Genome ◽  
Dna Viruses ◽  
Tissue Specific ◽  
B Virus ◽  
Tissue Specific Promoter ◽  
A Cell

AbstractGenome editing techniques such as CRISPR/Cas9 have both become common gene engineering technologies and have been applied to gene therapy. However, the problems of increasing the efficiency of genome editing and reducing off-target effects that induce double-stranded breaks at unexpected sites in the genome remain. In this study, we developed a novel Cas9 transduction system, Exci-Cas9, using an adenovirus vector (AdV). Cas9 was expressed on a circular molecule excised by the site-specific recombinase Cre and succeeded in shortening the expression period compared to AdV, which expresses the gene of interest for at least 6 months. As an example, we chose hepatitis B, which currently has more than 200 million carriers in the world and frequently progresses to liver cirrhosis or hepatocellular carcinoma. The efficiencies of hepatitis B virus genome disruption by Exci-Cas9 and Cas9 expression by AdV directly (Avec) were the same, about 80–90%. Furthermore, Exci-Cas9 enabled cell- or tissue-specific genome editing by expressing Cre from a cell- or tissue-specific promoter. We believe that Exci-Cas9 developed in this study is useful not only for resolving the persistent expression of Cas9, which has been a problem in genome editing, but also for eliminating long-term DNA viruses such as human papilloma virus.

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