Effect of Adenosine Injection Following Cerebral Reperfusion Ischemia on A1AR Gene Expression and Apoptosis in Brain Hippocampal Tissue of Male Wistar Rats

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Atossa Jozaei ◽  
Monireh Movahedi ◽  
Maryam Khosravi ◽  
Fereshteh Golab
Keyword(s):  
Gene Expression ◽  
Wistar Rats ◽  
Hippocampal Tissue ◽  
Male Wistar Rats ◽  
Cerebral Reperfusion

scholarly journals Upregulation of Kiss-1 and Gpr54 Genes Expression in Pituitary of Male Rats Following the Central Administration of Neuropeptide Y

2019 ◽  
Vol 4 (4) ◽  
pp. 137-142
Author(s):  
Vahid Azizi ◽  
Shahrbanoo Oryan ◽  
Homayuon Khazali ◽  
Abdolkarim Hosseini
Keyword(s):  
Gene Expression ◽  
Pituitary Gland ◽  
Neuropeptide Y ◽  
Wistar Rats ◽  
Third Ventricle ◽  
Male Rats ◽  
Control Group ◽  
Central Administration ◽  
Reproductive Axis ◽  
Male Wistar Rats

Introduction: The neuropeptide Y (NPY) in the neural circuits of the hypothalamus has a stimulating effect on reproductive activities in mammals. Kisspeptin (KiSS1) is a quintessential neurotransmitter in the reproductive axis which directly stimulates gonadotropin-releasing hormone neurons in the hypothalamus. The distribution of KiSS1 expressing cells in the pituitary was described previously. Despite earlier reports showing the KiSS1 receptor, G-protein coupled receptor 54 (GPR54) expression in the pituitary, the potential physiological roles of kisspeptin at this gland have remained obscure. Accordingly, this study investigated the role of NPY on the relative expression of Kiss1 and Gpr54 genes in the pituitary gland in male Wistar rats. Methods: In general, 20 male Wistar rats weighing 200-250 g in 4 groups (5 in each group) received saline, NPY (2.3 nM), BIBP3226 (NPY receptor antagonist, 7.8 nM), and NPY+ BIBP3226. Then, they received the simultaneous injection of these molecules through the third ventricle of the brain. Finally, the relative mean expressions of Kiss1 and Gpr54 genes in the anterior pituitary were quantitatively analyzed by the real-time polymerase chain reaction. Results: The central injection of NPY increased the relative mean expressions of Kiss1 and Gpr54 genes in the pituitary gland compared to the control group although the injection of BIBP3226 eradicated these effects. However, the gene expression of Gpr54 in the rats receiving NPY coupled with BIBP3226 in hypophysis in comparison to the group receiving only NPY demonstrated a significant reduction (P<0.05). Conclusion: Overall, the central injection of NPY stimulated the gene expression of Kiss1 and Gpr54 in the pituitary gland.

scholarly journals Triiodothyronine Improves Morphology and Up-regulates seladin-1 of neurospheres extracted from Subventricular Zone in Streptozotocin-induced rat model of Alzheimer's disease

2020 ◽  
Vol 6 (1) ◽  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Rat Model ◽  
Subventricular Zone ◽  
Wistar Rats ◽  
Culture Medium ◽  
Culture Media ◽  
Model Of Alzheimer’S Disease ◽  
Sh Group ◽  
Male Wistar Rats

Objectives: In this study, the effects of triiodothyronine (T3) on neurospheres isolated from SVZ of AD induced rats were examined. Methods: Eighteen male Wistar rats were classified into two groups: Sham (Sh) and STZ (Streptozotocin injected, 1.5 mg/kg in each lateral ventricle on days 1 and 3 after recovery). On day 21, the SVZ was extracted and neurospheres were cultured. T3 (50 nM) was added to the culture medium (STZ+T3 group) and then, the morphology and seladin-1 gene expression of neurospheres were evaluated. Results: The diameter and the number of neurospheres along with the gene expression of seladin-1 were significantly decreased in the STZ group compared to Sh group (P˂0.05) while the administration of T3 significantly (P˂0.05) increased all these parameters in the STZ group. Conclusion: STZ decreases the proliferation of stem cells extracted from SVZ and administration of T3 to the culture media improves the morphology and up-regulates the gene expression of seladin-1 of neurospheres.

Chlorpheniramine Maleate Induced Cardiotoxicity, Hepatotoxicity and Antioxidant Gene Expression Changes in Male Wistar Rats

2020 ◽  
Vol 16 (4) ◽  
pp. 351-366
Author(s):  
Sherifa S. Hamed ◽  
Sherine Abdel Sala ◽  
Manal F. El-Khad ◽  
Wafa A. AL-Megr ◽  
Zeinab K. Hassan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wistar Rats ◽  
Chlorpheniramine Maleate ◽  
Antioxidant Gene ◽  
Male Wistar Rats ◽  
Antioxidant Gene Expression

Fasting Status and Thermally Oxidized Sunflower Oil Ingestion Affect the Intestinal Antioxidant Enzyme Activity and Gene Expression of Male Wistar Rats

2010 ◽  
Vol 58 (4) ◽  
pp. 2498-2504 ◽  
Author(s):  
Raul Olivero David ◽  
Sara Bastida ◽  
Adriana Schultz ◽  
Laura González Torres ◽  
M. José González-Muñoz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Sunflower Oil ◽  
Wistar Rats ◽  
Antioxidant Enzyme Activity ◽  
Male Wistar Rats

scholarly journals Effect of sitagliptin on depression in male wistar rats

2019 ◽  
Vol 9 (1) ◽  
pp. 200
Author(s):  
Vidya M. Mahalmani ◽  
Anil P. Hogade ◽  
Sanjay K. Mishra
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Wistar Rats ◽  
Cytokine Gene Expression ◽  
Antidepressant Effect ◽  
Oxidative Stress Markers ◽  
Cytokine Gene ◽  
Stress Markers ◽  
Group I ◽  
Male Wistar Rats

Background: Growing evidence supports relationship between depression and inflammation. The hypothesis of involvement of inflammatory pathways in depression is supported by the findings of increased levels of proinflammatory cytokines. So, we decided to evaluate the effect of sitagliptin on depression using forced swim test (FST) and possible effects of sitagliptin on serum oxidative stress markers and cytokine gene expression in rat hippocampus.Methods: FST model was used to evaluate antidepressant effect in male wistar rats. Rats in group I (control group) were given normal saline, group II (standard group) were given fluoxetine, group III and IV (test groups) were given sitagliptin 5 mg/kg and sitagliptin 9 mg/kg respectively. All the drugs in all groups were given per orally. At the end, animals were sacrificed and blood was collected. Hippocampus of rat brain was dissected out. Serum oxidative stress markers and hippocampal pro inflammatory cytokine gene expression analysis was carried out.Results: Sitagliptin 5 mg/kg and 9 mg/kg showed reduction in depressive symptoms and hippocampal cytokine gene expression in comparison to control. In case of serum oxidative stress markers, there was statistically significant reduction in nitric oxide levels with stagliptin 9 mg/kg. Although there was a decrease in the levels of catalase and increase in the levels of glutathione with standard and test groups, the results were not statistically significant.Conclusions: The present study showed significant antidepressant effect activity of standard and test groups. Hence, further research should be carried out to substantiate above results.

scholarly journals RANK/RANKL/OPG Expression in Rapid Maxillary Expansion

2017 ◽  
Vol 28 (3) ◽  
pp. 296-300 ◽  
Author(s):  
Maya Fernanda Manfrin Arnez ◽  
Larissa Soares Nogueira Ribeiro ◽  
Gabriel Dessotti Barretto ◽  
Patrícia Maria Monteiro ◽  
Edilson Ervolino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Resorption ◽  
Wistar Rats ◽  
Rapid Maxillary Expansion ◽  
Metal Ring ◽  
Maxillary Expansion ◽  
Qrt Pcr ◽  
Tukey Test ◽  
Male Wistar Rats ◽  
Over Time

Abstract The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (a=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.

scholarly journals Effects of Eight Weeks of Resistance Training on Muscle Myostatin Gene Expression and Insulin Resistance in Male Wistar Rats with Type 2 Diabetes

2019 ◽  
Vol 6 (4) ◽  
pp. 9-16
Author(s):  
Majid Hassan Qomi ◽  
Sajad Arshadi ◽  
Abdolali Banayifar ◽  
Yaser Kazemzadeh ◽  
◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Resistance Training ◽  
Wistar Rats ◽  
Myostatin Gene ◽  
Male Wistar Rats

scholarly journals 8 Weeks of Resistance Training Effect on Myostatin Gene Expression of Myocardium in Healthy Male Wistar Rats

2016 ◽  
Vol 22 (2) ◽  
pp. 111-116
Author(s):  
Amir Rashidlamir ◽  
Mohammad Reza Basami ◽  
Seyyed Reza Attarzadeh Hosseini ◽  
keyvan Hejazi ◽  
Seyyed Mohamad Motevalli Anberani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Training ◽  
Wistar Rats ◽  
Training Effect ◽  
Healthy Male ◽  
Myostatin Gene ◽  
Male Wistar Rats
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