scholarly journals The Relationship of Class I Integron Gene Cassettes and the Multidrug-Resistance in Extended -Spectrum β-Lactamase Producing Isolates of Escherichia coli

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Alisha Akya ◽  
Roya Chegene Lorestani ◽  
Mosayeb Rostamian ◽  
Azam Elahi ◽  
Shokofe Baakhshii ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Class I ◽  
Gene Cassettes ◽  
Extended Spectrum ◽  
Relationship Of ◽  
The Relationship

scholarly journals Prevalence Of Extended Spectrum Beta-Lactamases (ESBLs)-Producing Escherichia Coli Isolated From UTI Patients Attending some Selected Hospitals In Minna, Nigeria

2021 ◽  
Vol 37 (2) ◽  
pp. 56-73
Author(s):  
F Iseghohi ◽  
J.C Igwe ◽  
M Galadima ◽  
A.F Kuta ◽  
A.M Abdullahi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Urine Samples ◽  
Antibiotics Resistance ◽  
Healthy Individuals ◽  
Beta Lactamases ◽  
E Coli ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Tract Infections

Globally, urinary tract infections are one of the most common infections in need of urgent clinical attention. The prevalence of extended spectrum beta-lactamases (ESBL)- producing Escherichia coli isolated from urine samples of some UTI patients and s of apparently healthy individuals in Minna, Nigeria, is investigated. Standard microbiological techniques were used to conduct this study. A total of 170 catch midstream urine samples submitted to the Medical Microbiology Laboratories of 4 different hospitals (and samples from healthy individuals) were randomly collected for 5 months and examined for microbial growths. Female patients (65.9%) submitted more urine samples for UTI test than their male counterpart (34.1%). The age ranges of 21 -30 (26.5%) and 31 - 40 (25.3%) had the highest percentages of infection rate while those within the ages 1- 10 (3.5%) and ≥ 71 (2.3%) were the least infected. This study observed a prevalence of 23.5% of E. coli in Minna metropolis and a significant number (30%) of healthy individuals (HI) was observed to harbor the E. coli in their urine. The isolates were highly susceptible to Gentamicin (65%), Ofloxacin (65%), Tetracycline (62.5%), Cotrimoxazole (62.5%), and Streptomycin (57.5%). Mildly susceptible to Pefloxacin (37.5%), Chloramphenicol (37.5%), and Ciprofloxacin (35%). There were significant resistance to most of the beta-lactames tested [Cefuroxime (80%), Amoxicillin (42.5%), Augmentin (40), Cefotaxime (20%) and Ceftaxidime (7.5%)]. Two of the isolates were resistant to all the 13 antibiotics tested; 70% (28) of the isolates had multiple antibiotics resistance index (MARI) ≥0.3. Multidrug resistance was expressed in 37.5% of the isolates tested. The study showed a vast resistant pool in the environment. Only 25% of the E. coli isolated from the urine samples produced beta-lactamases phenotypically, most of which expressed resistance to more than 5 of the antibiotics tested and had MARI of ≥ 0.5. Further evaluation showed that 25% (10/40) of the E. coli isolated from the UTI patients in Minna, Nigeria, were ESBL- producers and could harbor one or two of the genes. TEM gene was expressed in 70% (7) of the isolates that produced ESBL phenotypically, 60% 6) harbored CTXM gene, 20% (2) had the OXA gene while none of the bacteria harbored the SHV gene. The study established a 5.9% ESBL prevalence among the E. coli isolated from UTI in the environment studied. This study established that E. coli is one of the prevalent bacteri urea majorly isolated from UTI patients in Minna. The prevalent E. coli are multidrug resistant and could harbor more than one ESBL gene . keywords: Escherichia coli, Minna, UTI, ESBL, Multidrug resistance

Antimicrobial Resistance, Extended-Spectrum β-Lactamase Productivity, and Class 1 Integrons in Escherichia coli from Healthy Swine

2015 ◽  
Vol 78 (8) ◽  
pp. 1442-1450 ◽  
Author(s):  
KANJANA CHANGKAEW ◽  
APIRADEE INTARAPUK ◽  
FUANGFA UTRARACHKIJ ◽  
CHIE NAKAJIMA ◽  
ORASA SUTHIENKUL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Intestinal Flora ◽  
Class 1 Integron ◽  
Potential Health ◽  
Gene Cassettes ◽  
E Coli ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Class 1

Administration of antimicrobials to food-producing animals increases the risk of higher antimicrobial resistance in the normal intestinal flora of these animals. The present cross-sectional study was conducted to investigate antimicrobial susceptibility and extended-spectrum β-lactamase (ESBL)–producing strains and to characterize class 1 integrons in Escherichia coli in healthy swine in Thailand. All 122 of the tested isolates had drug-resistant phenotypes. High resistance was found to ampicillin (98.4% of isolates), chloramphenicol (95.9%), gentamicin (78.7%), streptomycin (77.9%), tetracycline (74.6%), and cefotaxime (72.1%). Fifty-four (44.3%) of the E. coli isolates were confirmed as ESBL-producing strains. Among them, blaCTX-M (45 isolates) and blaTEM (41 isolates) were detected. Of the blaCTX-M-positive E. coli isolates, 37 carried the blaCTX-M-1 cluster, 12 carried the blaCTX-M-9 cluster, and 5 carried both clusters. Sequence analysis revealed blaTEM-1, blaTEM-135, and blaTEM-175 in 38, 2, and 1 isolate, respectively. Eighty-seven (71%) of the 122isolates carried class 1 integrons, and eight distinct drug-resistance gene cassettes with seven different integron profiles were identified in 43 of these isolates. Gene cassettes were associated with resistance to aminoglycosides (aadA1, aadA2, aadA22, or aadA23), trimethoprim (dfrA5, dfrA12, or dfrA17), and lincosamide (linF). Genes encoding β-lactamases were not found in class 1 integrons. This study is the first to report ESBL-producing E. coli with a class 1 integron carrying the linF gene cassette in swine in Thailand. Our findings confirm that swine can be a reservoir of ESBL-producing E. coli harboring class 1 integrons, which may become a potential health risk if these integrons are transmitted to humans. Intensive analyses of animal, human, and environmental isolates are needed to control the spread of ESBL-producing E. coli strains.

scholarly journals Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteria

1971 ◽  
Vol 123 (4) ◽  
pp. 501-505 ◽  
Author(s):  
J. W. Dale
Keyword(s):  
Escherichia Coli ◽  
Host Species ◽  
Host Bacterium ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
R Factor ◽  
Relationship Of ◽  
The Relationship

1. The amino acid composition of the β-lactamase from E. coli (R-1818) was determined. 2. The R-1818 β-lactamase is inhibited by formaldehyde, hydroxylamine, sodium azide, iodoacetamide, iodine and sodium chloride. 3. The Km values for benzylpenicillin, ampicillin and oxacillin have been determined by using the R-factor enzyme from different host species. The same values were obtained, irrespective of the host bacterium. 4. The molecular weight of the enzyme was found to be 44600, and was the same for all host species. 5. The relationship of R-1818 and R-GN238 β-lactamases is discussed.

scholarly journals Comparison of the population occlusal characteristics in 3 Brazilian regions

2020 ◽  
Vol 9 (10) ◽  
pp. e1839108586
Author(s):  
Marcio da Costa Almeida ◽  
Paula Cotrin ◽  
Fabricio Pinelli Valarelli ◽  
Rodrigo Hermont Cançado ◽  
Ricardo Cesar Gobbi de Oliveira ◽  
...  
Keyword(s):  
Class Ii ◽  
Open Bite ◽  
Class I ◽  
Class Iii ◽  
Chi Square ◽  
Posterior Crossbite ◽  
Individual Traits ◽  
Orthodontic Patients ◽  
Relationship Of ◽  
The Relationship

The objective of this work was to determine the prevalence of individual traits of malocclusion in a sample of three Brazilian regions orthodontic patients and to detect interregional population differences in the prevalence of certain occlusal characteristics.  The present study was based on the examination of dental casts, intraoral photographs and panoramic radiographs of 947 orthodontic patients from 3 cities: 363 from Fortaleza (CE), 270 from Maringá (PR) and 314 from Bauru (SP), respectively, representing the Northeast, South and Southeast Brazilian regions. The relationship of the first maxillary and mandibular molars according to Angle’s classification, overjet, overbite, crowding, posterior crossbite and maxillary median diastema were examined. The chi-square and ANOVA tests were used to determine potential differences in the distribution of malocclusion when stratified Brazilian regions. Class I malocclusion was found in 499 (52.69%), Class II in 395 (41.71%) and Class III malocclusion in 53 (5,59%) subjects of all examined. Deep overbite (3.59%), midline diastemas (5.17%) and posterior crossbite (4.75%) were observed more frequently in Bauru; however, in Maringá, normal overbite (13.3%) and open bite (4.75%) were more prevalent. The results of this study showed that Class I malocclusion was the most prevalent, followed by Class II and Class III malocclusions. These occlusal relationships evaluated in the three Brazilian regions follow the same pattern of frequency as the result presented by the general population of the sample.

Analysis of the Relationship of Microbial Contamination with Temperature and Cleaning Frequency and Method of Domestic Refrigerators in Japan

2020 ◽  
Vol 83 (7) ◽  
pp. 1234-1240
Author(s):  
MIHO MORI ◽  
YOSHIKAZU SAKAGAMI ◽  
MEGUMI TANAKA ◽  
RYO INOUE ◽  
TORU JOJIMA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbial Contamination ◽  
Food Poisoning ◽  
Hygiene Management ◽  
Bottom Shelf ◽  
Relationship Of ◽  
The Relationship ◽  
And Staphylococcus Aureus ◽  
Poisoning Prevention

ABSTRACT Hygiene management of domestic refrigerators is an important aspect of food poisoning prevention. The aim of the present study was to confirm the relationship between microbial contamination and hygiene management by measuring microbial levels and investigating temperature and cleaning frequency and method of domestic refrigerators in Japan. We analyzed three internal sections (the egg compartment, bottom shelf, and vegetable drawer) of 100 domestic refrigerators in Japan. Salmonella, Listeria monocytogenes, and Yersinia enterocolitica were not found in any of the refrigerators, but coliforms and Escherichia coli were detected in more than one household, and Staphylococcus aureus was the most frequently isolated pathogen. The prevalences of these microorganisms had similar tendencies in all three sections sampled and were highest in the vegetable drawer. The temperature distribution in the refrigerators was also investigated, and a temperature >6.1°C (improper temperature) was found in 46.2% of the areas surveyed. Only 17% of the respondents cleaned their refrigerators monthly or more often, and this frequency was lower than that reported in other countries. Fifty percent of the respondents used only water to clean the refrigerator, 10% used only an alcohol or disinfecting wipe, and 8% used only a dry cloth. Although no significant correlations were found between microbial contamination and temperatures in refrigerators, correlations were found between microbial contamination and refrigerator cleaning frequency and/or method. To our knowledge, this is the first detailed survey concerning relationships between microbial contamination and hygiene management in domestic refrigerators in Japan. The data obtained can be used to promote food poisoning management in Japanese households. HIGHLIGHTS

The relationhip between occlusal factors and bruxism in permanent and mixed dentition in Turkish children

2001 ◽  
Vol 25 (3) ◽  
pp. 191-194 ◽  
Author(s):  
Saziye Sari ◽  
Hayriye Sonmez
Keyword(s):  
Permanent Dentition ◽  
Mixed Dentition ◽  
Class I ◽  
Primary Molars ◽  
Turkish Children ◽  
Angle Class ◽  
Relationship Of ◽  
The Relationship ◽  
Anterior Posterior

The aim of this study was to investigate the relationship between occlusal factors (overjet, overbite, Angle's Classification of molars and cuspids, the relationship of the primary molars, openbite, lateral openbite, scissorbite and crossbite) and bruxism in permanent and mixed dentition in Turkish children. For this reason 182 children with mixed dentition and 212 children with permanenth dentition were included in this study. Occlusal conditions were examined clinically and bruxism was assesed by using interview and questionnaires. Z Test was used to compare the results. It was found that in both dentition some occlusal factors related with bruxism (overjet > 6 mm, overbite > 5 mm, negative overjet, openbite in permanent dentition; overjet > 6 mm, overbite > 5 mm, scissorbite, anterior-posterior multiple teeth crossbite, Angle Class I occlusion in mixed dentition.

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