Utility of Glucose Transporter 1 in the Distinction of Benign and Malignant Thoracic and Abdominal Mesothelial Lesions

2012 ◽  
Vol 136 (7) ◽  
pp. 804-809 ◽  
Author(s):  
Stephen M. Lagana ◽  
Robert N. Taub ◽  
Alain C. Borczuk
Keyword(s):  
Malignant Mesothelioma ◽  
Predictive Value ◽  
Glucose Transporter ◽  
High Specificity ◽  
Tissue Microarrays ◽  
Lower Sensitivity ◽  
Positive Staining ◽  
Glucose Transporter 1 ◽  
Glut 1 ◽  
Reactive Hyperplasia

Context.—Malignant mesothelioma, of either peritoneum or pleura, is an uncommon cancer. The diagnosis is often difficult to make, in part because of the overlapping morphology of reactive and malignant mesothelial cells. Glucose transporter 1 (GLUT-1) is a glucose transporter typically found on erythrocytes, which is aberrantly expressed in various carcinomas. It has recently been reported as specific and sensitive in discriminating malignant pleural mesothelioma from reactive hyperplasia. The application of GLUT-1 staining in peritoneal mesothelioma has not been fully explored. Objective.—To determine if GLUT-1 staining is helpful in distinguishing abdominal mesotheliomas from benign, reactive mesothelial lesions and to further study its utility in the thorax. Design.—Tissue microarrays containing 135 abdominal malignant mesotheliomas and 30 malignant pleural mesotheliomas were stained with an antibody to GLUT-1, as were 56 reactive mesothelial lesions. Results.—The overall sensitivity and specificity for GLUT-1 in mesothelioma was 53% and 98%, respectively. The sensitivity in epithelioid malignant mesothelioma was 49% and in sarcomatoid/biphasic malignant mesothelioma, 66%. In the thorax, the sensitivity was 50% and in the abdomen it was 54%. The positive predictive value of GLUT-1 immunoreactivity was 98% and the negative predictive value was 40%. Conclusion.—Glucose transporter 1 staining of thoracic mesotheliomas showed high specificity but lower sensitivity than previously reported. Abdominal malignant mesotheliomas showed similar results. Because of low sensitivity, only positive staining is informative. In both sites, the utility of the stain was limited by nonspecific staining (eg, in necrotic areas) as well as bright labeling of erythrocytes and occasional lymphoid elements. Despite these limitations, GLUT-1 can help differentiate malignant mesothelioma from reactive benign mesothelium.

scholarly journals Highly expressed IMP3 and GLUT-1 in combination with the loss of BAP1 expression is useful for differentiating malignant mesothelioma from reactive mesothelial hyperplasia

2020 ◽  
Author(s):  
Hongjun Zhu ◽  
Qing Hu ◽  
Daishan Jiang ◽  
Hua Huang ◽  
Jian Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Diagnostic Accuracy ◽  
Malignant Mesothelioma ◽  
Glucose Transporter ◽  
High Specificity ◽  
Glucose Transporter 1 ◽  
Expression Levels ◽  
Glut 1 ◽  
Mrna Binding Protein ◽  
Mrna Binding

Abstract Background Our study aims to evaluate how IHC staining of BRCA1 associated protein-1 (BAP1), insulin-like growth factor mRNA binding protein 3 (IMP3), and glucose transporter-1 (GLUT-1) can help to differentiate MM from reactive mesothelial hyperplasia (RMH). Methods The expression levels of BAP1, IMP3, and GLUT-1 were investigated using immunohistochemistry (IHC) in 38 MMs and 32 RMHs. Results BAP1 was lost in 20 (52.6%) of the 38 MMs. IMP3 and GLUT-1 were positive in 26 (68.4%) and 25 (65.8%), respectively, of the 38 MMs. The results of IHC analyses of BAP1, IMP3, and GLUT-1 indicated that these markers were characterized by a 100% specificity with sensitivities of 52.63, 68.42, and 65.79%, respectively. In addition, BAP1, IMP3, and GLUT-1 combined showed 100% specificity and the highest sensitivity (81.58%). Meanwhile, loss of BAP1 expression is related to a better prognosis. Conclusions Overexpressed IMP3 and GLUT-1 in combination with the loss of BAP1 showed high specificity to MM that was different from that in RMH and improved diagnostic accuracy, especially in MM.

Differential glycolytic and glycogenogenic transduction pathways in male and female bovine embryos produced in vitro

2012 ◽  
Vol 24 (2) ◽  
pp. 344 ◽  
Author(s):  
M. Garcia-Herreros ◽  
I. M. Aparicio ◽  
D. Rath ◽  
T. Fair ◽  
P. Lonergan
Keyword(s):  
Glucose Metabolism ◽  
Protein Expression ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Glycogen Synthase ◽  
Bovine Embryos ◽  
High Concentration ◽  
Glucose Transporter 1 ◽  
Male And Female ◽  
Glut 1

Previous studies have shown that developmental kinetic rates following IVF are lower in female than in male blastocysts and that this may be related to differences in glucose metabolism. In addition, an inhibition of phosphatidylinositol 3-kinase (PI3-K) inhibits glucose uptake in murine blastocysts. Therefore, the aim of this study was to identify and compare the expression of proteins involved in glucose metabolism (hexokinase-I, HK-I; phosphofructokinase-1, PFK-1; pyruvate kinase1/2, PK1/2; glyceraldehyde-3-phosphate dehydrogenase, GAPDH; glucose transporter-1, GLUT-1; and glycogen synthase kinase-3, GSK-3) in male and female bovine blastocysts to determine whether PI3-K has a role in the regulation of the expression of these proteins. Hexokinase-I, PFK-1, PK1/2, GAPDH and GLUT-1 were present in bovine embryos. Protein expression of these proteins and GSK-3 was significantly higher in male compared with female blastocysts. Inhibition of PI3-K with LY294002 significantly decreased the expression of HK-I, PFK-1, GAPDH, GSK-3 A/B and GLUT-1. Results showed that the expression of glycolytic proteins HK-I, PFK-1, GAPDH and PK1/2, and the transporters GLUT-1 and GSK-3 is regulated by PI3-K in bovine blastocysts. Moreover, the differential protein expression observed between male and female blastocysts might explain the faster developmental kinetics seen in males, as the expression of main proteins involved in glycolysis and glycogenogenesis was significantly higher in male than female bovine embryos and also could explain the sensitivity of male embryos to a high concentration of glucose, as a positive correlation between GLUT-1 expression and glucose uptake in embryos has been demonstrated.

Overexpression of Glucose Transporter-1 (GLUT-1) Predicts Poor Prognosis in Epithelial Ovarian Cancer

2013 ◽  
Vol 31 (9) ◽  
pp. 607-615 ◽  
Author(s):  
Hanbyoul Cho ◽  
You Sun Lee ◽  
Julie Kim ◽  
Joon-Yong Chung ◽  
Jae-Hoon Kim
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Poor Prognosis ◽  
Glucose Transporter ◽  
Glucose Transporter 1 ◽  
Glut 1

scholarly journals Expression of Glucose Transporter 1 Confers Susceptibility to Human T-Cell Leukemia Virus Envelope-Mediated Fusion

2005 ◽  
Vol 79 (7) ◽  
pp. 4150-4158 ◽  
Author(s):  
Ayse Kubra Coskun ◽  
Richard E. Sutton
Keyword(s):  
T Cell ◽  
Glucose Transporter ◽  
Leukemia Virus ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Glucose Transporter 1 ◽  
Glut 1 ◽  
Human T Cell ◽  
T Cell Leukemia Virus ◽  
Mdbk Cells

ABSTRACT Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus identified and causes both adult T-cell leukemia/lymphoma and tropical spastic paraparesis/HTLV-1-associated myelopathy, among other disorders. In vitro, HTLV-1 has an extremely broad host cell tropism in that it is capable of infecting most mammalian cell types, although at the same time viral titers remain relatively low. Despite years of study, only recently has a bona fide candidate cellular receptor, glucose transporter 1 (glut-1), been identified. Although glut-1 was shown to bind specifically to the ectodomain of HTLV-1 and HTLV-2 envelope glycoproteins, which was reversible with small interfering RNA directed against glut-1, cellular susceptibility to HTLV upon expression of glut-1 was not established. Here we show that expression of glut-1 in relatively resistant MDBK cells conferred increased susceptibility to both HTLV-1- and HTLV-2-pseudotyped particles. glut-1 also markedly increased syncytium formation in MDBK cells after exposure to HTLV-1. Another assay also demonstrated HTLV-1 envelope-cell fusion in the presence of glut-1. Taken together, these results provide additional evidence that glut-1 is a receptor for HTLV.

scholarly journals Factors Impacting the Performance Characteristics of Bile Duct Brushings: A Clinico-Cytopathologic Analysis of 253 Patients

2018 ◽  
Vol 142 (7) ◽  
pp. 863-870 ◽  
Author(s):  
Ezgi Hacihasanoglu ◽  
Bahar Memis ◽  
Burcin Pehlivanoglu ◽  
Vaidehi Avadhani ◽  
Alexa A. Freedman, ◽  
...  
Keyword(s):  
Bile Duct ◽  
Predictive Value ◽  
High Specificity ◽  
Daily Practice ◽  
Performance Characteristics ◽  
Lower Sensitivity ◽  
Current State ◽  
Risk Of Malignancy ◽  
Low Sensitivity ◽  
Sensitivity Specificity

Context.— Literature on factors impacting bile duct brushings (BDBs) performance characteristics remain limited. Objective.— To capture the current state of daily practice with BDB sign-out. Design.— Two hundred fifty-three of 444 BDBs signed out by more than 7 cytopathologists, with histopathologic and/or clinical follow-up of at least 18 months, were examined. Results.— One hundred thirty-five of 253 BDBs (53%) had histologically confirmed malignancies, 22 (9%) had cancer-related deaths, and 96 (38%) were benign. Cytologic diagnoses in the 444 BDBs were nondiagnostic (11 [2.5%]), negative (284 [64%]), atypical (62 [13.9%]), suspicious (34 [7.7%]), and malignant (53 [11.9%]). Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of malignancy detection were 35%, 100%, 100%, 58%, and 66%, respectively. When atypical, suspicious, and malignant (ASM) categories were combined, sensitivity increased (58%), specificity and PPV dropped (97%), and accuracy increased (73%). Carcinoma type (bile-duct versus pancreatic-ductal) had no effect on accuracy (P = .60) or diagnostic class (P = .84), nor did time of performance (first 7.5 versus latter 7.5 years, P = .13). Interestingly, ThinPrep + cell block (n = 41) had higher sensitivity (61%) and lower specificity (80%) than ThinPrep only (versus 51% and 100%, respectively). Sensitivity and specificity were higher (47% and 100%) in nonstented than stented specimens (59% and 97%). Relative risk of malignancy for “suspicious” (2.30) and “atypical” (2.28) categories was lower but not very different from that of “malignant” category (2.41). Conclusions.— Bile duct brushings had fairly low sensitivity but high specificity and PPV with no false positives. Sensitivity almost doubled and specificity dipped minimally when ASM categories were combined, highlighting the need for better classification criteria for atypical/suspicious cases. Higher specificity, PPV, NPV, and accuracy but lower sensitivity in stented BDBs suggest that they be called malignant only when evidence is overwhelmingly convincing.

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