scholarly journals Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 Isolated from Eucommia ulmoides Oliv.

2013 ◽  
Vol 12 (15) ◽  
pp. 1862-1868 ◽  
Author(s):  
Ting Ding ◽  
Wei Wei Sun ◽  
Yong Xia Qi ◽  
Hai Yang Jiang
Keyword(s):  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Antimicrobial Activities ◽  
Eucommia Ulmoides ◽  
Active Components

scholarly journals Isolation and antimicrobial effects of endophytic fungi from Edgeworthia chrysantha

2015 ◽  
Vol 10 (3) ◽  
pp. 529 ◽  
Author(s):  
Huawei Zhang ◽  
Chuanfeng Ruan ◽  
Xuelian Bai
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Human Pathogens ◽  
Antimicrobial Effects ◽  
Staphyloccocus Aureus

<p>Ten fungal strains isolated from <em>Edgeworthia chrysantha</em>, one of traditional medicinal plants in China, were evaluated their antimicrobial activities against three human pathogens, <em>Escherichia coli, Staphyloccocus aureus and Candida albicans</em>, and two phytopathogens, <em>Rhizoctonia cerealis</em> and <em>Colletotrichum gloeosporioides</em>. The results indicated that most ethyl acetate extracts of fermentation broth of these fungal endophytes had stronger antimicrobial activities than their fermentation broth. Among these endophytic strains, both fermentation broth and the ethyl acetate extract of strain D showed the strongest inhibitory effects on all pathogens. Strains 5-19 and BZ also exhibited potent antibacterial activities. However, other strains had weak or no antimicrobial effect. This was the first report on the isolation and antimicrobial effects of endophytic fungi from <em>E. chrysantha</em>.   </p><p> </p>

scholarly journals Isolation and antifungal screening of endophytic fungi from Erigeron canadensis

2017 ◽  
Vol 12 (3) ◽  
pp. 256 ◽  
Author(s):  
Xuelian Bai ◽  
Ting Zhou ◽  
Tongfei Lai ◽  
Yicong Li ◽  
Jiale Chai ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Video Clip ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Antifungal Activities ◽  
Erigeron Canadensis ◽  
Full Screen

<p class="Abstract">Sixteen fungal strains isolated from the Erigeron canadensis, one of traditional Chinese medicines used to treat the pathogenic infection and dysentery, were evaluated for their antifungal activities against one human pathogen Candida albicans, and two phytopathogens, Colletotrichum fructicola and Rhizoctonia cerealis. The bioassay results indicated that the ethyl acetate extract of the fermentation broth of these fungal endophytes had stronger antimicrobial activities. Among these endophytic strains, the ethyl acetate extracts of strains NPR003 and NPR005 showed the strongest inhibitory effects and has potential application in the discovery of new antifungal agents. This was the first report on the isolation of endophytic fungi from E. canadensis and evaluation of their antifungal activities.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">1  min 4 sec   <a href="https://www.youtube.com/v/EdohQU3anR4">Full Screen</a>   <a href="https://www.youtube.com/watch?v=EdohQU3anR4">Alternate</a></p>

scholarly journals Impact of Altitudinal Variation on the Phytochemical Profile, Anthelmintic and Antimicrobial Activity of Two Pinus Species

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3170
Author(s):  
Wafaa M. Elkady ◽  
Mariam H. Gonaid ◽  
Miriam F. Yousif ◽  
Mahmoud El-Sayed ◽  
Hind A. N. Omar
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Biological Activities ◽  
Anthelmintic Activity ◽  
Antimicrobial Activities ◽  
Pinus Pinea ◽  
Active Components ◽  
Pinus Species ◽  
Bacteria And Fungi

Active components from natural sources are the current focus in most pharmacological research to provide new therapeutic agents for clinical use. Essential oils from the Pinus species have been traditionally used in medicine. This study aimed to investigate the chemical profile of two Pinus species, Pinus halepensis L. and Pinus pinea Mill, from different altitudes in Libya and study the effect of environmental conditions on the biological activities of essential oils. A clevenger apparatus was used to prepare the essential oils by hydrodistillation. Analyses were done using GC/MS. Anthelmintic and antimicrobial activities were tested against the earthworm Allolobophora caliginosa, gram-positive bacteria, gram-negative bacteria, and fungi. Different chemical profiles were observed among all tested essential oils, and terpenes were the most dominant class. All studied essential oils from the Pinus species exhibited a remarkable anthelmintic activity compared to the standard piperazine citrate drug. Pinus halepensis from both altitudes showed broad-spectrum antimicrobial activity against all tested microorganisms, while Pinus pinea was effective against only Escherichia coli. From these findings, one can conclude that there are variations between studied species. The essential oil compositions are affected by environmental factors, which consequently affect the anthelmintic and antimicrobial activity.

scholarly journals Profiling Secondary Metabolites of Cocoa (Theobroma cacao L.) Endophytic Fungi Lasiodiplodia pseudotheobromae PAK-7 and Lasiodiplodia theobromae TN-R-3 and Their Antimicrobial Activities

2020 ◽  
pp. 47-56 ◽  
Author(s):  
M. Chaithra ◽  
S. Vanitha ◽  
A. Ramanathan ◽  
V. Jegadeeshwari ◽  
V. Rajesh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Tamil Nadu ◽  
Statistical Design ◽  
Mevalonic Acid ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Chemical Compositions

Aims: To determine the chemical composition of secondary metabolite of cocoa endophytic fungi L. pseudotheobromae PAK-7, L. theobromae TN-R-3 and their anti-oomycete activities. Statistical Design: Multivariate analysis. Place and Duration of Study: Department of Plant Pathology, TNAU, Coimbatore, Tamil Nadu from April 2018 to December 2019. Methodology: Lipophilic extracellular secondary metabolites were extracted using ethyl acetate as a solvent and their chemical composition was detected by Gas Chromatography-Mass Spectrometry (GC-MS) and identified by NIST library and Pub Chem databases. Results: Metabolic profiling of cocoa endophytic fungi  L. pseudotheobromae PAK-7  and  L. theobromae TN-R-3  showed the presence of eleven peaks representing nine compounds. The most abundant compound observed were Acetic acid, 3-methyl-6-oxo-9oxabicyclo[3.3.1]non-2-yl ester, 2H-Pyran-2-one, tetrahydro-4-hydroxy-6-pentyl-, Melezitose, Ethyl à-d-glucopyranoside  collectively representing 58.01% area. In comparison to L. pseudotheobromae PAK-7 GC-MS analysis of L. theobromae TN-R-3 exhibited the presence of 29 peaks. The most abundant compounds were dl-Mevalonic acid lactone, Methyl 6-O-[1-methylpropyl]-á-d-galactopyranoside, 2H-Pyran-2-one, tetrahydro-4-hydroxy-6-pentyl-, Melezitose, Ethyl à-d-glucopyranoside, 1,6-Anhydro-á-d-talopyranose collectively representing 60.47% of the total area. Conclusion: Chemical compositions and anti-oomycete activities of crude secondary metabolites of L. pseudotheobromae PAK-7, L. theobromae TN-R-3 differed entirely depending on the property and abundance of bioactive metabolites.

scholarly journals Anticancer Properties of Eugenol: A Review

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7407
Author(s):  
Ali T. Zari ◽  
Talal A. Zari ◽  
Khalid Rehman Hakeem
Keyword(s):  
Antimicrobial Activities ◽  
Syzygium Aromaticum ◽  
Active Components ◽  
Cancer Treatments ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
Anticancer Effects ◽  
The World ◽  
Cancer Types ◽  
Anti Inflammatory

Conventional cancer treatments have shown several unfavourable adverse effects, as well as an increase in anticancer drug resistance, which worsens the impending cancer therapy. Thus, the emphasis is currently en route for natural products. There is currently great interest in the natural bioactive components from medicinal plants possessing anticancer characteristics. For example, clove (Syzygium aromaticum L.) (Family Myrtaceae) is a highly prized spice that has been historically utilized as a food preservative and for diverse medical uses. It is reckoned amongst the valued sources of phenolics. It is indigenous to Indonesia but currently is cultivated in various places of the world. Among diverse active components, eugenol, the principal active component of S. aromaticum, has optimistic properties comprising antioxidant, anti-inflammatory, and anticancer actions. Eugenol (4-allyl-2-methoxyphenol) is a musky oil that is mainly obtained from clove. It has long been utilized all over the world as a result of its broad properties like antioxidant, anticancer, anti-inflammatory, and antimicrobial activities. Eugenol continues to pique investigators’ interest because of its multidirectional activities, which suggests it could be used in medications to treat different ailments. Anticancer effects of eugenol are accomplished by various mechanisms like inducing cell death, cell cycle arrest, inhibition of migration, metastasis, and angiogenesis on several cancer cell lines. Besides, eugenol might be utilized as an adjunct remedy for patients who are treated with conventional chemotherapy. This combination leads to a boosted effectiveness with decreased toxicity. The present review focuses on the anticancer properties of eugenol to treat several cancer types and their possible mechanisms.

scholarly journals TLC Bioautography-guided Isolation and Antimicrobial, Antifungal Effects of 12 Alkaloids from Hylomecon japonica Roots§

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200
Author(s):  
Xuanji Xue ◽  
Hui Zhang ◽  
Xinxin Zhang ◽  
Xia Liu ◽  
Ke Xi ◽  
...  
Keyword(s):  
Antimicrobial Activities ◽  
Cell Integrity ◽  
Active Components ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Tlc Bioautography ◽  
Broth Microdilution Method ◽  
Antimicrobial Mechanism ◽  
Chinese Medicinal Plant

Hylomecon japonica (Thunb.) Prantl. is a Chinese medicinal plant whose active components and their antimicrobial mechanism was studied by TLC bioautography-guided isolation and identification of 12 alkaloids and their antimicrobial activities evaluated using the standard broth microdilution method. The benzophenanthridine isoquinoline alkaloids showed the strongest inhibitory activity against Gram-positive bacteria. Analysis of conductivity changes and observation of TEM images revealed that the antimicrobial mechanism involved cell integrity damage and cell division arrest.

Screening of endophytic fungal metabolites from Cola nitida leaves for antimicrobial activities against clinical isolates of Pseudomonas aeruginosa

2020 ◽  
Vol 4 (3) ◽  
pp. 161-166
Author(s):  
David C. Nwobodo ◽  
Chibueze P. Ihekwereme ◽  
Festus B. C. Okoye
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
High Performance ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Array Detector ◽  
Mortality And Morbidity ◽  
Cola Nitida ◽  
Fungal Extracts

AbstractEndophytic fungi of selected Nigerian plants are important sources of bioactive products with enormous potentials for the discovery of new drug molecules for drug development. Pseudomonas aeruginosa is one of the major causes of healthcare-associated bacterial infections, leading to increased mortality and morbidity. In this study, isolated endophytic fungi from Cola nitida were screened for anti-pseudomonas properties. Endophytic fungi associated with healthy leaves of C. nitida were isolated using standard methods. Fungi were identified through their morphological, cultural and microscopic characteristics. The fungi were subjected to solid-state fermentation and secondary metabolites extracted using ethyl acetate and concentrated under vacuum. The crude extracts were screened for antimicrobial activity against clinical and laboratory strains of Pseudomonas aeruginosa using the agar diffusion method. The bioactive components of the fungal extracts were identified using High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) analysis. Three endophytic fungi; Acremonium sp., Aspergillus sp. and Trichophyton sp. were isolated. At 1 mg/ml, extracts of the three fungi displayed antipseudomonal activity with inhibition zone diameter ranging from 6 - 4 mm. HPLC-DAD analysis revealed the presence of compounds, such as 4-hydroxyphenyl acetate. indole-3-acetic acid, and protocatechuic acid among others in the fungal extracts. The findings in this study reveal that endophytic fungi associated with C. nitida possess promising antipseudomonal properties. This finding can open new doors for the discovery of new agents against P. aeruginosa.

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