scholarly journals Isolation and antifungal screening of endophytic fungi from Erigeron canadensis

2017 ◽  
Vol 12 (3) ◽  
pp. 256 ◽  
Author(s):  
Xuelian Bai ◽  
Ting Zhou ◽  
Tongfei Lai ◽  
Yicong Li ◽  
Jiale Chai ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Video Clip ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Antifungal Activities ◽  
Erigeron Canadensis ◽  
Full Screen

<p class="Abstract">Sixteen fungal strains isolated from the Erigeron canadensis, one of traditional Chinese medicines used to treat the pathogenic infection and dysentery, were evaluated for their antifungal activities against one human pathogen Candida albicans, and two phytopathogens, Colletotrichum fructicola and Rhizoctonia cerealis. The bioassay results indicated that the ethyl acetate extract of the fermentation broth of these fungal endophytes had stronger antimicrobial activities. Among these endophytic strains, the ethyl acetate extracts of strains NPR003 and NPR005 showed the strongest inhibitory effects and has potential application in the discovery of new antifungal agents. This was the first report on the isolation of endophytic fungi from E. canadensis and evaluation of their antifungal activities.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">1  min 4 sec   <a href="https://www.youtube.com/v/EdohQU3anR4">Full Screen</a>   <a href="https://www.youtube.com/watch?v=EdohQU3anR4">Alternate</a></p>

scholarly journals Isolation and antimicrobial effects of endophytic fungi from Edgeworthia chrysantha

2015 ◽  
Vol 10 (3) ◽  
pp. 529 ◽  
Author(s):  
Huawei Zhang ◽  
Chuanfeng Ruan ◽  
Xuelian Bai
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Human Pathogens ◽  
Antimicrobial Effects ◽  
Staphyloccocus Aureus

<p>Ten fungal strains isolated from <em>Edgeworthia chrysantha</em>, one of traditional medicinal plants in China, were evaluated their antimicrobial activities against three human pathogens, <em>Escherichia coli, Staphyloccocus aureus and Candida albicans</em>, and two phytopathogens, <em>Rhizoctonia cerealis</em> and <em>Colletotrichum gloeosporioides</em>. The results indicated that most ethyl acetate extracts of fermentation broth of these fungal endophytes had stronger antimicrobial activities than their fermentation broth. Among these endophytic strains, both fermentation broth and the ethyl acetate extract of strain D showed the strongest inhibitory effects on all pathogens. Strains 5-19 and BZ also exhibited potent antibacterial activities. However, other strains had weak or no antimicrobial effect. This was the first report on the isolation and antimicrobial effects of endophytic fungi from <em>E. chrysantha</em>.   </p><p> </p>

scholarly journals Anti-snake venom potential of Clerodendrum serratum extracts on Bungarus caeruleus and Daboia russelii venom

2018 ◽  
Vol 13 (2) ◽  
pp. 187
Author(s):  
Shwetha Vasudev ◽  
Sunil Shivajirao More ◽  
Gautham Santhekadur Annappa ◽  
Veena Sunil More
Keyword(s):  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Snake Venom ◽  
Chronic Conditions ◽  
Video Clip ◽  
Snake Bite ◽  
Phospholipase A ◽  
Methanolic Extracts ◽  
Full Screen ◽  
Fibrinogenolytic Activity

<p class="Abstract">Anti-snake venom therapy is the only treatment for snake bite but leads to acute and chronic conditions which may be severe. The medicinal plants have gained importance over years to find an effective alternative to anti-snake venom. The present study focused on evaluating the potential of <em>Clerodendrum serratum</em> for the anti-snake venom activity. Phytochemicals were extracted from the <em>C. serratum</em> with different solvents. The ethyl acetate and methanolic extracts were found to neutralize the major enzyme toxins (phospholipase A<sub>2</sub>, protease and hyaluronidase) of <em>Bungarus caeruleus</em> and <em>Daboia russelii</em> venom at a concentration of 100 µg/mL. The fibrinogenolytic activity of both the venoms were neutralized. The study proves that the plant <em>C. serratum</em> possesses certain compounds which inhibit the toxins present in the venom of <em>B. caeruleus</em> and <em>D. russelii</em>.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Hyaluronidase assay:   3 min  30 sec   <a href="https://www.youtube.com/v/Xz5C6bbwgW0">Full Screen</a>    <a href="https://www.youtube.com/watch?v=Xz5C6bbwgW0">Alternate</a></p>

scholarly journals Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9342
Author(s):  
Chao An ◽  
Saijian Ma ◽  
Xinwei Shi ◽  
Wenjiao Xue ◽  
Chen Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
High Performance ◽  
Fungal Endophytes ◽  
Folk Medicine ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Qinling Mountains

Endophytic fungi have been emerged as fruitful resources for producing structurally fascinating and biologically active secondary metabolites. However, endophytic fungi from medicinal plants of Qinling Mountains–the most important natural climatic boundary between the subtropical and warm temperate zones of China with an astonishingly high level of biodiversity–have rarely been explored as potential sources of novel fungal species and active secondary metabolites. In this study, a total of 371 fungal colonies were successfully isolated from 510 tissue segments of the medicinal Tupistra chinensis Baker collected from Qinling Mountains, China. Roots of T. chinensis Baker are used as a folk medicine to ameliorate pharyngitis and treat rheumatic diseases. A total of 100 representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), seven classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Microbotryomycetes, Agaricomycetes, Leotiomycetes, Mortierellomycetes), and at least 35 genera. The genera of Collectotrichum (IF, 29.92%), Fusarium (IF, 8.36%), Aspergillus (IF, 8.09%), and Dactylonectria (IF, 5.39%) were most frequently isolated from the tissues of T. chinensis Baker. The Species Richness Index (S, 65) and the Shannon-Wiener Index (H′, 3.7914) indicated that T. chinensis Baker harbored abundant fungal resources. Moreover, five isolates were potential new taxa because of low similarity of ITS sequences ranged from 95.09%∼96.61%. Fifteen out of 100 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Two important lead compounds produced by two stains (F8047 and F8075) with high antimicrobial activities were identified using high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC–QTOF MS) analyses. In addition, it was noteworthy that the strain F8001, which may be a potential new species, showed antimicrobial activity and should be investigated further. Overall, these results indicated that the endophytic fungi from T. chinensis Baker could be exploited as a novel source of bioactive compounds.

scholarly journals Fungal endophytes of some medicinal plant growing in northwestern coast of Egypt: Isolation, identification and two-way clustering analysis of its antimicrobial activity

2021 ◽  
Author(s):  
Mohammad Magdy El-Metwally ◽  
Atia Mohamed Eisa ◽  
Amal AI Mekawey ◽  
Samy F. Mahmoud ◽  
Yasser El Halmouch
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Fungal Endophytes ◽  
Diversity Indices ◽  
Antimicrobial Activities ◽  
Penicillium Expansum ◽  
Nicotiana Glauca ◽  
Northwestern Coast

Abstract One of hidden mine of antibiotics is endophytic fungi especially that inhibited medicinal plants. In this regard, leaves, stems, fruits and bulbs of some commonly medicinal plants growing in Northwestern coast of Egypt were subjected for isolation of endophytic fungi with screening study of its antimicrobial activity. Practically, more than one hundred (101) endophytic fungal species isolated from Scorpiurus muricatus, Mellilotus indicus, Lotus polyphyllos, Ononis vaginalis, Nicotiana glauca, Lycium europaeum, Asphodelus aestivus, Echium angustifolium,Fagonia cretica, Pancratium maritimum,and Carduus getulus were tested in vitro for their antimicrobial activities against E. coli, Pseudomonas argenosa, Staphylococcus aureus, Bacillus subtilus, Candida albicanus, Candida glabrata, Penicillium expansum, Aspergillus flavus. The values of applied diversity indices revealed significant differences in presence, absence and abundance among endophytic fungal isolates. To the best of our knowledge, the present study is the first to report of Alternaria pluriseptata as endophytic species with most dominant and most active in its anitimicrobial activity among the isolated species. Seven distinctive groups were revealed from the two-way cluster analysis showing the intensity of antimicrobial activity against tested pathogens: Twenty-five percent of the isolates (26 strains assembled together in group V) exhibited no antimicrobial activity against all tested pathogens while six percent (6 isolates) assembled in group VII revealed high antimicrobial activity against five pathogens.

scholarly journals Chemical composition and biological activity of Nepeta cilicica

2017 ◽  
Vol 12 (2) ◽  
pp. 20 ◽  
Author(s):  
Gökalp İşcan ◽  
Fatih Göger ◽  
Betül Demirci ◽  
Yavuz Bülent Köse
Keyword(s):  
Essential Oil ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Video Clip ◽  
Radical Scavenging ◽  
Inhibitory Effects ◽  
Plant Materials ◽  
Aerial Parts ◽  
Full Screen ◽  
Main Components

<p>In the present study, aerial parts of <em>Nepeta cilicica</em> were hydro-distilled for the essential oil. Furthermore, air-dried plant materials were extracted with methanol. According to GC-MS results, 35 compounds were characterized in the oil (totally 92%). Caryophyllene oxide, β-caryophyllene and spathulenol (28.2, 8.9 and 4.2%) were found as main components of the oil. Sixteen compounds were detected by LC/MS, most of which were caffeoylquinic acid, luteolin and apigenin derivatives, in methanolic extract of the N. cilicica. Apart from DPPH radical scavenging activity, inhibitory effects on bacterial and yeast growth of essential oil and the methanolic extract were evaluated. Twenty-four different pathogenic bacterial and <em>Candida</em> strains were tested by M7-A7 and CLSI M27-A2 protocols respectively. The crude extract showed better inhibitory effects against bacteria strains than the essential oil. Both oil and the extract demonstrated strong inhibition on <em>C. tropicalis</em> at a concentration of 47.0 µg/mL (MIC).</p><p><strong>Video Clip of Methodology</strong>:</p><p>CLSI microdilution broth method: 3 min 46 sec   <a href="https://youtube.com/v/PBADMG2zG1c">Full Screen</a>   <a href="https://youtube.com/watch?v=PBADMG2zG1c">Alternate</a></p>

Study on Limonium aureum Endophytic Fungi and its Antibacterial Activity

2013 ◽  
Vol 634-638 ◽  
pp. 1071-1075 ◽  
Author(s):  
Xiao Feng Liu ◽  
Jiao Ling Qian ◽  
Peng Lin ◽  
Hong Yan Xue ◽  
Lin Yang
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Filter Paper ◽  
Fermentation Broth ◽  
Test Bacterium ◽  
General Method

Objective To research antibacterial activity of Limonium aureum endophytic fungi. Methods The endophytic fungi of Limonium aureum root was isolated by general method. After incubation, fermentation broth and biomass were extracted by ethyl acetate, n-bothanol and ethanol. The bactefiostatic test was experimented with six different bacterium．The method used was filter paper slice tests. Results 21 endophytes were obtained. After bactefiostatic testing, there were 31 samples with antibaceterial activity against at least one test bacterium he MIC of extraction from E101 and E201 against test bacteria was 0.25mg/mL.

scholarly journals Diversity Estimation and Antimicrobial Activity of Culturable Endophytic Fungi from Litsea cubeba (Lour.) Pers. in China

Forests ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 33 ◽  
Author(s):  
Fei Wu ◽  
Dingchao Yang ◽  
Linping Zhang ◽  
Yanliu Chen ◽  
Xiaokang Hu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Forest Ecosystems ◽  
Fungal Endophytes ◽  
Pathogenic Fungi ◽  
Antimicrobial Activities ◽  
Plant Pathogenic Fungi ◽  
Litsea Cubeba ◽  
Stem Leaf ◽  
Potential Use

Endophytes are important components of forest ecosystems, and have potential use in the development of medical drugs and the conservation of wild medicinal plants. This study aimed to examine the diversity and antimicrobial activities of endophytic fungi from a medicinal plant, Litsea cubeba (Lour.) Pers. The results showed that a total of 970 isolates were obtained from root, stem, leaf, and fruit segments of L. cubeba. All the fungal endophytes belonged to the phylum Ascomycota and could be classified into three taxonomic classes, nine orders, twelve families, and seventeen genera. SF15 (Colletotrichum boninense) was the dominant species in L. cubeba. Leaves harbored a greater number of fungal endophytes but lower diversity, while roots harbored the maximum species diversity of endophytic fungi. For the antimicrobial activities, seventeen isolates could inhibit the growth of plant pathogenic fungi, while the extracts of six endophytes showed antimicrobial activity to all the tested pathogenic fungi. Among these endophytes, SF22 (Chaetomium globosum) and SF14 (Penicillium minioluteum) were particularly effective in inhibiting seven plant pathogenic fungi growths and could be further explored for their potential use in biotechnology, medicine, and agriculture.

Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

2021 ◽  
pp. 86-95
Author(s):  
Baiq Maylinda Gemantari ◽  
Fitra Romadhonsyah ◽  
Arief Nurrochmad ◽  
Subagus Wahyuono ◽  
Puji Astuti
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Cytotoxic Activities ◽  
Dpph Assay ◽  
Nitrobenzyl Alcohol ◽  
Layer Chromatography ◽  
Coleus Amboinicus

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R*,6S*,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.

Isolation of Endophytic Fungi from Ephedra intermedia and Research Antibacterial Activity of Secondary Metabolite Produced by the Fungi

2014 ◽  
Vol 881-883 ◽  
pp. 488-492 ◽  
Author(s):  
Lin Yang ◽  
Wei Juan Li ◽  
Jing Long ◽  
Ai Mei Yang ◽  
Zhong Duo Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Endophytic Fungi ◽  
Methyl Alcohol ◽  
Ethyl Acetate Extract ◽  
Fermentation Broth

20 strains of endophytic fungi were isolated from roots and stems of Ephedra intermedia. After fermentation these strains, fermentation broth and mycelium were extracted by ethyl acetate, n-butanol and methyl alcohol, producing 60 extracts. Then the antibacterial activity of these extracts was researched, the result found that 33 extracted samples could evidently inhibit the growth of six different bacteria, accounted for 55%. The MIC of ethyl acetate extract of J109 was 0.15625mg/mL to five test bacterial expect Streptococcus uberi.

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