scholarly journals Effect of ascorbic acid on mercuric chloride-induced changes on the cerebral cortex of wistar rats

2013 ◽  
Vol 1 (1) ◽  
pp. 23-29 ◽  
Author(s):  
AO Ibegbu ◽  
AA Animoku ◽  
M Ayuba ◽  
D Brosu ◽  
SA Adamu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebral Cortex ◽  
Mercuric Chloride ◽  
Wistar Rats ◽  
Induced Changes

scholarly journals Histomorphological effect of ascorbic acid on mercury chloride-induced changes on the cerebellum of adult wistar rats

2014 ◽  
Vol 31 (04) ◽  
pp. 219-224 ◽  
Author(s):  
A. Ibegbu ◽  
A. Animoku Abdulrazaq ◽  
Ayuba Micheal ◽  
Brosu Daniel ◽  
A. Adamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebellar Cortex ◽  
Wistar Rats ◽  
Oral Route ◽  
Mercury Chloride ◽  
Average Weight ◽  
The Central Nervous System ◽  
Living Organisms ◽  
Induced Changes ◽  
Acid Administration

Abstract Introduction. Mercury is one of the most hazardous environmental contaminants to living organisms and the central nervous system has been shown to be the main target. Objective. The present work was aimed at evaluating the effect of ascorbic acid on mercury chloride-induced changes on the cerebellar cortex of adult Wistar rats. Material and method. Thirty Wistar rats of average weight of 200g and were randomly divided into 6 groups of 5 rats each. The animals in Group 1 (control) were administered with distilled water, Groups 2 and 3 were administered with 52mg/kg and 26.25mg/kg body weight of HgCl respectively while Groups 4 and 5 were administered with 52mg/kg of HgCl and 5mg/kg of ascorbic acid and 26.25gm/kg of HgCl and 5mg/kg of ascorbic acid respectively, while Group 6 was administered with 5mg/kg of ascorbic acid. The administration was through oral route, daily for 3 weeks. Results. The result of the biochemical parameters showed a significant increase (P < 0.05) on the mean SOD and LPO values after the administration of mercury chloride and Ascorbic acid. Histological observation of the cerebellar cortex, showed normal histo-morphology in Groups 1 and 6 while, the cerebellum in Groups 2, 3, 4 and 5 showed some degenerative, necrotic and cellular changes. Conclusion. However, ascorbic acid administration has shown to ameliorate the induced degenerative changes in the cerebellum caused by mercury chloride toxicity in Wistar rats.

scholarly journals The effect of ascorbic acid on mercury-induced changes on the histomorphology of the cerebellum of adult wistar rats

2014 ◽  
Vol 3 (9) ◽  
pp. 9-15
Author(s):  
AO Ibegbu ◽  
AA Animoku ◽  
M, Ayuba ◽  
D Brosu ◽  
SA Adamu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Induced Changes

Ameliorative effect of ascorbic acid on mercury chloride-induced changes on the spleen of adult wistar rats

2014 ◽  
Vol 13 (2) ◽  
pp. 60
Author(s):  
AugustineOseloka Ibegbu ◽  
Ayuba Micheal ◽  
AAnimoku Abdulrazaq ◽  
Brosu Daniel ◽  
AAdamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Mercury Chloride ◽  
Ameliorative Effect ◽  
Induced Changes

scholarly journals Effect of Ascorbic acid on Mercury-induced changes on the liver in Adult Wistar Rats

2014 ◽  
Vol 13 (10) ◽  
pp. 10-16 ◽  
Author(s):  
Ibegbu A.O ◽  
Ayuba Micheal ◽  
Animoku Abdulrazaq A ◽  
Brosu Daniel ◽  
Adamu Sadeeq A ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Induced Changes

Ascorbic acid improves extrapyramidal syndromes and corpus striatal degeneration induced by dopamine-2 receptor inhibition in Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sirajo U. Mujittapha ◽  
Murtala Kauthar ◽  
Ishola O. Azeez ◽  
John C. Oyem
Keyword(s):  
Ascorbic Acid ◽  
Normal Saline ◽  
Wistar Rats ◽  
Corpus Striatum ◽  
Extrapyramidal Symptoms ◽  
Motor Deficit ◽  
Cylinder Test ◽  
Extrapyramidal Syndromes ◽  
Dopamine 2 Receptor ◽  
Inhibition Group

AbstractObjectivesThe prolonged uses of fourth-generation antipsychotics have been implicated in inducing extrapyramidal syndromes characterized by the motor deficit. This was attributed to the loss of dopamine-2 receptor (D2R) signaling. However, ascorbic acid (SVCT2R stimulation) in the brain is proposed to modulate D2R activity. We, therefore, investigated the beneficial roles of ascorbic acid in improving the extrapyramidal symptoms seen in D2R loss.MethodsTwenty adult male Wistar rats of average weight 200 g were distributed randomly into four groups. The control (NS) received normal saline for 28 days, Untreated D2R inhibition group (−D2R) received normal saline for seven days and then subsequently received chlorpromazine for 21 days, D2R inhibition group treated with ascorbic acid (−D2R+SVCT2R) received chlorpromazine for 21 days and was subsequently treated with ascorbate for seven days while the withdrawal group (WG) received chlorpromazine for 21 days and subsequently received normal saline for seven days. Motor deficits were assessed using a rotarod and cylinder test. The corpus striatum was harvested, processed, and stained using H&E and Nissl stains. Cellular density was analyzed using Image J software 1.8.0.ResultsMotor deficit was observed in −D2R animals administered chlorpromazine with less improvement in WG compared to control (p<0.05) in both rotarod and cylinder test. Ascorbic acid (SVCT2R stimulation) significantly (p<0.001) improved the latency of fall and climbing attempts observed in −D2R animals. The density of basophilic trigoid bodies was significantly (p<0.001) restored in −D2R+SVCT2R group, suggesting recovery of neural activity in the corpus striatum. Moreover, the hallmarks of neuronal degeneration were less expressed in the ascorbic acid treatment groups.ConclusionsAscorbic acid putatively ameliorates extrapyramidal symptoms observed in D2R blockage by chlorpromazine in Wistar rats.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

Analysis of the mechanism of -amphetamine-and apomorphine-induced changes of ascorbic acid catabolism in discrete brain areas of the rat

1991 ◽  
Vol 23 (3) ◽  
pp. 295-306 ◽  
Author(s):  
M DESOLE ◽  
M MIELE ◽  
G ESPOSITO ◽  
P ENRICO ◽  
G DENATELE ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Brain Areas ◽  
Induced Changes

Determination of the L-ascorbic acid requirements in Wistar osteogenic disorder Shionogi rats for prolonged carcinogenesis experiments

1996 ◽  
Vol 30 (4) ◽  
pp. 337-346 ◽  
Author(s):  
S. W. Y. Chan ◽  
P. C. Reade
Keyword(s):  
Animal Model ◽  
Ascorbic Acid ◽  
Drinking Water ◽  
Normal Level ◽  
Physical Condition ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Supplement Group ◽  
Palatal Mucosa

Wistar Shionogi rats of the ( od/od) substrain with the osteogenic disorder are unable to synthesize L-ascorbic acid ( L-AA) and appear to be an appropriate animal model for studying the effect of L-AA in carcinogenesis. To determine the minimal L-AA requirements of these animals for prolonged survival in a satisfactory physical condition during experimentation, four concentrations of L-AA (0.33 g/l, 0.67 g/l, 1.67 g/l and 3.33 g/l) were administered via drinking water to four groups of animals ( n=2). Their water intake per cage was recorded three times weekly and the plasma L-AA levels were determined at the start, after 2, 4, 8 and 12 weeks and at the termination of the experiment. To simulate the procedures to be undertaken in oral mucosal carcinogenesis experiments, the animals were gently restrained and a designated amount of sterile NaCl was applied to the palatal mucosa three times a week for 26 weeks. The L-AA supplement group with the lowest concentration (0.33 g/l L-AA) achieved mean plasma levels of 7 ± 1.38 μM, approximately one-eighth that of the normal level (mean plasma L-AA level in outbred Wistar rats was found to be 58 ± 3 μM) whilst those in the higher supplement group (3.33 g/l L-AA) achieved a mean of 18 ± 1.25 μM. All of the animals employed in the present study survived for 26 weeks and showed no clinical signs of L-AA deficiency during this period.

Changes in the Cortex Neurons in Single and Fractional Gamma Radiation

2021 ◽  
Vol 66 (4) ◽  
pp. 18-24
Author(s):  
I. Ushakov ◽  
Vladimir Fyodorov
Keyword(s):  
Nervous System ◽  
Cerebral Cortex ◽  
Radiation Exposure ◽  
Comparative Assessment ◽  
Male Rats ◽  
Fractionated Irradiation ◽  
Radiation Induced ◽  
Post Radiation ◽  
Induced Changes ◽  
The Brain

Purpose: Comparative assessment of radiation-induced changes in neurons of the cerebral cortex after a single and fractionated exposure to ionizing radiation in doses of 0.1 – 1.0 Gy. Material and methods. The study was carried out in compliance with the rules of bioethics on 180 white outbred male rats at the age of 4 months. by the beginning of the experiment, exposed to a single or fractionated exposure to γ-quanta of 60Co in total doses of 0.1; 0.2; 0.5 and 1.0 Gy. Neuromorphological and histochemical methods were used to assess morphometric and tinctorial parameters of nerve cells, as well as changes in the content of protein and nucleic acids in neurons in the early and late periods of the post-radiation period. Using one-way analysis of variance, a comparative assessment of neuromorphological indicators under various modes of radiation exposure is given. Results: In the control and irradiated animals throughout their life, undulating changes in the indicators of the state of the neurons of the brain occur with a gradual decrease by the end of the experiment. Despite a number of features of the dynamics of neuromorphological parameters, these irradiation regimes do not cause functionally significant changes in the neurons of the cortex. However, in some periods of the post-radiation period, the changes under the studied irradiation regimes were multidirectional and did not always correspond to age control. Significant differences in the response of neurons to these modes of radiation exposure in the sensory and motor areas of the cerebral cortex have not been established. Conclusion: No functionally significant radiation-induced changes in neurons were found either with single or fractionated irradiation. At the same time, different modes of irradiation in general caused the same type of changes in neurons. However, in some periods of observation, changes in neuromorphological parameters under the studied irradiation regimes were not unidirectional and differed from age control, which indicates a possible risk of disturbances in the functioning of the nervous system against the background of other harmful and dangerous factors.

The Study of Electroacupuncture on Cerebral Blood Flow in Rats With and Without Cerebral Ischemia

2006 ◽  
Vol 34 (02) ◽  
pp. 351-361 ◽  
Author(s):  
Ching-Liang Hsieh ◽  
Qwang-Yuen Chang ◽  
I-hsin Lin ◽  
Jaung-Geng Lin ◽  
Chung-Hsiang Liu ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Cortex ◽  
Cerebral Blood Flow ◽  
Cerebral Ischemia ◽  
Peripheral Blood ◽  
Sprague Dawley ◽  
Calcitonin Gene ◽  
Cerebral Ischemic ◽  
Induced Changes ◽  
Common Carotid Arteries

Electroacupuncture (EA) is widely used to treat disorders of the nervous system, such as stroke. The aim of the present study was to investigate the effect of EA on cerebral blood flow (CBF) in cerebral ischemic rats. We developed an animal model of cerebral ischemia (CI) by occluding the blood flow of both common carotid arteries in Sprague-Dawley (SD) rats; 2 or 15 Hz EA was applied to both Zusanli acupoints. The levels of nitric oxide (NO) in the peripheral blood and amounts of calcitonin gene-related peptide (CGRP) in the cerebral cortex and thalamus were measured. In addition, L-N (G)-nitro arginine methyl ester (L-NAME) was used to measure the changes in CBF induced by EA in rats with and without CI. The results indicated that both 2 and 15 Hz EA increase the mean CBF in rats with and without CI. However, neither 2 nor 15 Hz EA induced changes in levels of NO in peripheral blood or changes in CGRP levels in cerebral cortex and thalamus. In addition, L-NAME did not change the increase in CBF. We concluded that both 2 and 15 Hz EA at both Zusanli acupoints induced the increase of CBF in rats with and without CI. Whether the effect of EA is related to NO or CGRP will be investigated in a future study.

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