scholarly journals Histomorphological effect of ascorbic acid on mercury chloride-induced changes on the cerebellum of adult wistar rats

2014 ◽  
Vol 31 (04) ◽  
pp. 219-224 ◽  
Author(s):  
A. Ibegbu ◽  
A. Animoku Abdulrazaq ◽  
Ayuba Micheal ◽  
Brosu Daniel ◽  
A. Adamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebellar Cortex ◽  
Wistar Rats ◽  
Oral Route ◽  
Mercury Chloride ◽  
Average Weight ◽  
The Central Nervous System ◽  
Living Organisms ◽  
Induced Changes ◽  
Acid Administration

Abstract Introduction. Mercury is one of the most hazardous environmental contaminants to living organisms and the central nervous system has been shown to be the main target. Objective. The present work was aimed at evaluating the effect of ascorbic acid on mercury chloride-induced changes on the cerebellar cortex of adult Wistar rats. Material and method. Thirty Wistar rats of average weight of 200g and were randomly divided into 6 groups of 5 rats each. The animals in Group 1 (control) were administered with distilled water, Groups 2 and 3 were administered with 52mg/kg and 26.25mg/kg body weight of HgCl respectively while Groups 4 and 5 were administered with 52mg/kg of HgCl and 5mg/kg of ascorbic acid and 26.25gm/kg of HgCl and 5mg/kg of ascorbic acid respectively, while Group 6 was administered with 5mg/kg of ascorbic acid. The administration was through oral route, daily for 3 weeks. Results. The result of the biochemical parameters showed a significant increase (P < 0.05) on the mean SOD and LPO values after the administration of mercury chloride and Ascorbic acid. Histological observation of the cerebellar cortex, showed normal histo-morphology in Groups 1 and 6 while, the cerebellum in Groups 2, 3, 4 and 5 showed some degenerative, necrotic and cellular changes. Conclusion. However, ascorbic acid administration has shown to ameliorate the induced degenerative changes in the cerebellum caused by mercury chloride toxicity in Wistar rats.

Ameliorative effect of ascorbic acid on mercury chloride-induced changes on the spleen of adult wistar rats

2014 ◽  
Vol 13 (2) ◽  
pp. 60
Author(s):  
AugustineOseloka Ibegbu ◽  
Ayuba Micheal ◽  
AAnimoku Abdulrazaq ◽  
Brosu Daniel ◽  
AAdamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Mercury Chloride ◽  
Ameliorative Effect ◽  
Induced Changes

scholarly journals The effect of ascorbic acid on mercury-induced changes on the histomorphology of the cerebellum of adult wistar rats

2014 ◽  
Vol 3 (9) ◽  
pp. 9-15
Author(s):  
AO Ibegbu ◽  
AA Animoku ◽  
M, Ayuba ◽  
D Brosu ◽  
SA Adamu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Induced Changes

scholarly journals Effect of ascorbic acid on mercuric chloride-induced changes on the cerebral cortex of wistar rats

2013 ◽  
Vol 1 (1) ◽  
pp. 23-29 ◽  
Author(s):  
AO Ibegbu ◽  
AA Animoku ◽  
M Ayuba ◽  
D Brosu ◽  
SA Adamu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebral Cortex ◽  
Mercuric Chloride ◽  
Wistar Rats ◽  
Induced Changes

scholarly journals Weight Evaluation and Serum Indices Effect of Potash Administration on the Uterus of Pregnant Wistar Rat

2019 ◽  
Vol 4 (7) ◽  
Keyword(s):  
Physical Activity ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Oral Route ◽  
Control Group ◽  
Estrogen Level ◽  
Ad Libitum ◽  
Group 5 ◽  
Group 2 ◽  
Induced Changes

Potash known as potassium carbonate (K2 CO3 ) is a mixture of salt with other components including impurities which coexist in mineral and salt is highly consumed in various forms by pregnant women. The aim of this research is to evaluate the weight and serum indices of potash on the uterus of pregnant wistar rat with the specific objectives of determining the effect of potash on the progesterone and estrogen level and weight index of pregnant wistar rat. A total of Twenty-five albino Wistar rat with weights ranging from (180-300g) were used and allocated into five groups of five animals each (4 females and a male) designated as groups 1, 2, 3, 4, and 5. The experimental groups 2, 3, 4, and 5 were administered different doses of potash. The animals were allowed for a period of one week for acclimatization under normal temperature (270C -300C) which they were being feed with normal feed (grower’s mash) and water ad libitum for one (1) week. Administration of potash were through the oral route. Group 1 served as the control group and was administered distilled water only. Group 2 received 300mg/kg of potash plus feed and water was given ad libitum for 7 days after detection of pregnancy. Group 3 received 600mg/kg of potash plus feed and water was given ad libitum. Group 4 received 900mg/kg of potash plus feed and water was given ad libitum. Group 5 received 1200mg/kg of potash plus feed and water was given ad libitum. After analysis from histological procedures, the progesterone and estrogen level of pregnant wistar rat were evaluated and observed that treatment with potash induced changes. However, the level of progesterone activities increased in the test groups (2, 3, and 5) when compared to the control group. As for estrogen level, the increased level was observed to be progressive in the test groups, with the highest seen with the 1200mg/kg group as well as the physical activity of the wistar rats. At the end of the study, the results showed that Potash alters progesterone and estrogen level as well as the physical activity of the wistar rats.

scholarly journals Effect of Ascorbic acid on Mercury-induced changes on the liver in Adult Wistar Rats

2014 ◽  
Vol 13 (10) ◽  
pp. 10-16 ◽  
Author(s):  
Ibegbu A.O ◽  
Ayuba Micheal ◽  
Animoku Abdulrazaq A ◽  
Brosu Daniel ◽  
Adamu Sadeeq A ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Wistar Rats ◽  
Induced Changes

Ascorbic acid improves extrapyramidal syndromes and corpus striatal degeneration induced by dopamine-2 receptor inhibition in Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sirajo U. Mujittapha ◽  
Murtala Kauthar ◽  
Ishola O. Azeez ◽  
John C. Oyem
Keyword(s):  
Ascorbic Acid ◽  
Normal Saline ◽  
Wistar Rats ◽  
Corpus Striatum ◽  
Extrapyramidal Symptoms ◽  
Motor Deficit ◽  
Cylinder Test ◽  
Extrapyramidal Syndromes ◽  
Dopamine 2 Receptor ◽  
Inhibition Group

AbstractObjectivesThe prolonged uses of fourth-generation antipsychotics have been implicated in inducing extrapyramidal syndromes characterized by the motor deficit. This was attributed to the loss of dopamine-2 receptor (D2R) signaling. However, ascorbic acid (SVCT2R stimulation) in the brain is proposed to modulate D2R activity. We, therefore, investigated the beneficial roles of ascorbic acid in improving the extrapyramidal symptoms seen in D2R loss.MethodsTwenty adult male Wistar rats of average weight 200 g were distributed randomly into four groups. The control (NS) received normal saline for 28 days, Untreated D2R inhibition group (−D2R) received normal saline for seven days and then subsequently received chlorpromazine for 21 days, D2R inhibition group treated with ascorbic acid (−D2R+SVCT2R) received chlorpromazine for 21 days and was subsequently treated with ascorbate for seven days while the withdrawal group (WG) received chlorpromazine for 21 days and subsequently received normal saline for seven days. Motor deficits were assessed using a rotarod and cylinder test. The corpus striatum was harvested, processed, and stained using H&E and Nissl stains. Cellular density was analyzed using Image J software 1.8.0.ResultsMotor deficit was observed in −D2R animals administered chlorpromazine with less improvement in WG compared to control (p<0.05) in both rotarod and cylinder test. Ascorbic acid (SVCT2R stimulation) significantly (p<0.001) improved the latency of fall and climbing attempts observed in −D2R animals. The density of basophilic trigoid bodies was significantly (p<0.001) restored in −D2R+SVCT2R group, suggesting recovery of neural activity in the corpus striatum. Moreover, the hallmarks of neuronal degeneration were less expressed in the ascorbic acid treatment groups.ConclusionsAscorbic acid putatively ameliorates extrapyramidal symptoms observed in D2R blockage by chlorpromazine in Wistar rats.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

scholarly journals Potential Role of Trace Elements (Al, Cu, Zn, and Se) in Multiple Sclerosis Physiopathology

2020 ◽  
Vol 27 (4) ◽  
pp. 163-177
Author(s):  
Mohammad Sadegh Hesamian ◽  
Nahid Eskandari
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
Trace Elements ◽  
Plasma Cells ◽  
Peripheral Tolerance ◽  
The Central Nervous System ◽  
Living Organisms ◽  
Tolerance Breakdown ◽  
Peripheral Immune Cells

Multiple sclerosis (MS) is an unpredictable disease of the central nervous system. The cause of MS is not known completely, and pathology is specified by involved demyelinated areas in the white and gray matter of the brain and spinal cord. Inflammation and peripheral tolerance breakdown due to Treg cell defects and/or effector cell resistance are present at all stages of the disease. Several invading peripheral immune cells are included in the process of the disease such as macrophages, CD8+ T cells, CD4+ T cells, B cells, and plasma cells. Trace elements are known as elements found in soil, plants, and living organisms in small quantities. Some of them (e.g., Al, Cu, Zn, Mn, and Se) are essential for the body’s functions like catalysts in enzyme systems, energy metabolism, etc. Al toxicity and Cu, Zn, and Se toxicity and deficiency can affect the immune system and following neuron inflammation and degeneration. These processes may result in MS pathology. Of course, factors such as lifestyle, environment, and industrialization can affect levels of trace elements in the human body.

scholarly journals β-Elemene Suppresses Obesity-Induced Imbalance in the Microbiota-Gut-Brain Axis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 704
Author(s):  
Yingyu Zhou ◽  
Wanyi Qiu ◽  
Yimei Wang ◽  
Rong Wang ◽  
Tomohiro Takano ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
High Fat Diet ◽  
Brain Regions ◽  
Cerebral Metabolites ◽  
The Central Nervous System ◽  
Cerebral Inflammation ◽  
Regulatory Effects ◽  
Induced Changes ◽  
Pearson Correlations ◽  
Glucose Cycle

As a kind of metabolically triggered inflammation, obesity influences the interplay between the central nervous system and the enteral environment. The present study showed that β-elemene, which is contained in various plant substances, had effects on recovering the changes in metabolites occurring in high-fat diet (HFD)-induced obese C57BL/6 male mice brains, especially in the prefrontal cortex (PFC) and hippocampus (HIP). β-elemene also partially reversed HFD-induced changes in the composition and contents of mouse gut bacteria. Furthermore, we evaluated the interaction between cerebral metabolites and intestinal microbiota via Pearson correlations. The prediction results suggested that Firmicutes were possibly controlled by neuron integrity, cerebral inflammation, and neurotransmitters, and Bacteroidetes in mouse intestines might be related to cerebral aerobic respiration and the glucose cycle. Such results also implied that Actinobacteria probably affected cerebral energy metabolism. These findings suggested that β-elemene has regulatory effects on the imbalanced microbiota-gut-brain axis caused by obesity and, therefore, would contribute to the future study in on the interplay between cerebral metabolites from different brain regions and the intestinal microbiota of mice.

Sign in / Sign up

Export Citation Format

Share Document