Antimicrobial effects of essential oil from Pinus koraiensis Sieb. et Zucc. needles in the bioflims

Pinecone is a traditional folk herb, which has been used in China for many years. In this paper, the essential oil from Pinus koraiensis pinecones (PEO) was obtained by hydrodistillation and 41 compounds were identified by gas chromatography–mass spectrometry (GC-MS), mainly including α-Pinene (40.91%), Limonene (24.82%), and β-Pinene (7.04%). The purpose of this study was to investigate the anti-tumor activity of PEO on MGC-803 cells and its mechanism. Anti-tumor experiments in vitro showed PEO could significantly inhibit the proliferation and migration of MGC-803 cells, and it also could arrest the cell cycle in the G2/M phase, decrease the mitochondrial membrane potential, and induce apoptosis. Finally, the effects of PEO on genes expression on MGC-803 cells were analyzed by RNA sequencing, and results showed that after treatment with PEO, 100 genes were up-regulated, and 57 genes were down-regulated. According to the KEGG pathway and GSEA, FAT4, STK3, LATS2, YAP1, and AJUBA were down-regulated, which were related to HIPPO signaling pathway. Real-time PCR and western blot further confirmed the results of RNA sequencing. These results indicated that PEO may exert anti-tumor activity via the HIPPO/YAP signaling pathway. The anti-tumor mechanism of this oil can be further studied, which is important for the development of anti-tumor drugs.

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Evaluations on Deodorization Effect and Anti-oral Microbial Activity of Essential Oil from Pinus koraiensis

Korean Journal of Plant Resources ◽

10.7732/kjpr.2014.27.1.001 ◽

2014 ◽

Vol 27 (1) ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Hyun Jung Hwang ◽

Jung-Sik Yu ◽

Ha Yeon Lee ◽

Dong-Joo Kwon ◽

Woong Han ◽

...

Keyword(s):

Essential Oil ◽

Microbial Activity ◽

Pinus Koraiensis

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Chemical analysis and in vitro antimicrobial effects and mechanism of action of Trachyspermum copticum essential oil against Escherichia coli

Asian Pacific Journal of Tropical Medicine ◽

10.1016/j.apjtm.2017.07.006 ◽

2017 ◽

Vol 10 (7) ◽

pp. 663-669 ◽

Cited By ~ 7

Author(s):

Wei Huang ◽

Jian-Qing Wang ◽

Hai-Yan Song ◽

Qian Zhang ◽

Guang-Fa Liu

Keyword(s):

Escherichia Coli ◽

Chemical Analysis ◽

Essential Oil ◽

Mechanism Of Action ◽

Antimicrobial Effects ◽

Trachyspermum Copticum

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Antimicrobial effects of edible nano-composite based on bean pod shell gum, nano-TiO2, and Mentha pulegium essential oil

Journal of Applied Biology & Biotechnology ◽

10.7324/jabb.2019.70612 ◽

2019 ◽

Vol 7 (6) ◽

pp. 75-78

Keyword(s):

Essential Oil ◽

Mentha Pulegium ◽

Nano Tio2 ◽

Nano Composite ◽

Antimicrobial Effects

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Possible Tracheal Relaxant and Antimicrobial Effects of the Essential Oil of Ethiopian Thyme Species (Thymus serrulatus Hochst. ex Benth.): A Multiple Mechanistic Approach

Frontiers in Pharmacology ◽

10.3389/fphar.2021.615228 ◽

2021 ◽

Vol 12 ◽

Author(s):

Najeeb Ur Rehman ◽

Mohd Nazam Ansari ◽

Tesfay Haile ◽

Aman Karim ◽

Khalil Y Abujheisha ◽

...

Keyword(s):

Essential Oil ◽

Guinea Pig ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Response Curves ◽

Relaxant Effect ◽

Antimicrobial Effects ◽

Low Concentrations ◽

Pde Inhibition ◽

The Right

The genus Thymus is traditionally used for the treatment of hyperactive airways complaints. The purpose of the current study is to investigate the potential tracheal relaxant effect and possible mechanism(s) of the essential oil of Thymus serrulatus (TS Oil) in isolated guinea pig tracheal tissues. The essential oil was obtained from the fresh erial parts of Thymus serrulatus, and its phyto-components were identified by GC-MS analysis. Guinea pig tracheal preparations were used for testing the tracheal relaxant effect of TS Oil with the determination of the mechanism(s) involved in this relaxation. GC-MS findings reveal that terpenes, fragrance constituents, saponins, and higher fatty acids are present in TS Oil. In isolated guinea pig trachea, TS Oil inhibited carbachol (CCh, 1 µM) and K+ (80 mM)-induced contractions in a pattern similar to that of dicyclomine. TS Oil, at 0.3 mg/ml, shifted parallel CCh-curves towards the right, followed by a non-parallel shift at higher concentration (1 mg/ml), thus suppressing maximum response in the same manner as produced by dicyclomine. Pretreatment of tissues with TS Oil (1 and 3 mg/ml) also produced a rightward shift of Ca++ concentration-response curves (CRCs) in the same manner as caused by verapamil. Further, TS Oil at low concentrations (0.3 and 1 mg/ml) shifted isoprenaline-induced inhibitory CRCs towards the left and increased cAMP levels in isolated tracheal homogenates similar to papaverine, a phosphodiesterase (PDE) inhibitor. In the antimicrobial assay performed by the agar well diffusion method, TS Oil was found most active against Candida albicans and Staphylococcus aureus where the zone of inhibition measured was 28 mm. Additionally, there was little difference between standard strains of gram-positive and gram-negative bacteria. However, methicillin-resistant S. aureus (MRSA) showed a small zone of inhibition as compared to standard strains (22 mm). From these results, it can be concluded that the essential oil of T. serrulatus has the potential to produce antimicrobial effects while causing tracheal relaxation mediated possibly by anticholinergic effects, Ca++ channel blockade, and PDE inhibition whereas additional mechanism(s) cannot be ruled out.

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Antimicrobial Effect of Zataria Essential Oil on the Skin Bacteria in Wistar Rats

Complementary Medicine Journal ◽

10.32598/cmja.10.1.945.1 ◽

2020 ◽

Vol 10 (1) ◽

pp. 56-67

Author(s):

Soheila Faramarz Isfahanian ◽

◽

Maryam Sadrnia ◽

Sima Nasri ◽

Hamid Sobhanian ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Essential Oil ◽

Staphylococcus Epidermidis ◽

Wistar Rats ◽

Antimicrobial Effect ◽

Bacterial Strains ◽

Antimicrobial Effects ◽

Skin Bacteria ◽

Allergy Test

Objective: Zataria is one of the native plants of Iran which is widely used for the treatment of diseases among Iranians. In this study, we investigated the antimicrobial effects of Zataria essential oil on the skin bacteria in rats. Methods: Bacterial strains were isolated from the skin of 6 wistar rats and the antimicrobial effects of Zataria essential oil were evaluated by disk diffusion and microbroth dilution methods. In-vivo tests were performed to evaluate the antimicrobial effect of the essential oil by microbial culture as well as allergy tests on the skin of experimental rats compared to controls. Results: Three bacterial strains were isolated from the skin of rats identified as Staphylococcus aureus, Corynebacterium and Staphylococcus epidermidis. Minimum Growth Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the two strains of Staphylococcus aureus and Corynebacterium were obtained 0.39 and 0.78 mg/ml, while for Staphylococcus epidermidis, they were 0.195 and 0.39 mg/ml, respectively. In-vivo test results showed the antibacterial effect of the essential oil on the skin bacteria and no inflammatory effects were observed under the allergy test. Conclusion: Zataria essential oil has antimicrobial effects on the skin infections in lower concentrations. The use of this essential oil as an antiseptic and preservative in cosmetics is recommended instead of chemical preservatives that generally have skin side effects.

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